Restriction map for pF1KSDA0886
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       Asp700I     
Asp718I     AspEI       AspI        AviII       BamHI       
BbrPI       BbvCI       BcgI        BfrI        BglI        
BglII       BlpI        Bpu1102I    BsaAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspMI       BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    EcoO65I     EcoRV       EcoT22I     
FseI        FspI        HgaI        HpaI        KpnI        
MluI        NcoI        NheI        NotI        NruI        
NsiI        NspV        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PvuI        PvuII       RcaI        RsrII       SbfI        
SexAI       SfiI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          342  GTCGAC               GT^[AC][GT]AC
AccIII       2136  TCCGGA               T^CCGGA
AclI         2571  AACGTT               AA^CGTT
AflIII       1387  ACATGT               A^C[GA][CT]GT
AscI          223  GGCGCGCC             GG^CGCGCC
AseI         2047  ATTAAT               AT^TAAT
AsnI         2047  ATTAAT               AT^TAAT
AvrII         955  CCTAGG               C^CTAGG
BalI         2873  TGGCCA               TGG^CCA
BciVI        2680  GTATCC               GTATCC(6/5)
BlnI          955  CCTAGG               C^CTAGG
BsaWI        2136  TCCGGA               [AT]^CCGG[AT]
BseAI        2136  TCCGGA               T^CCGGA
BsgI         3300  CTGCAC               GTGCAG(16/14)
BspEI        2136  TCCGGA               T^CCGGA
BspLU11I     1387  ACATGT               A^CATGT
BssHII        224  GCGCGC               G^CGCGC
BstAPI        928  GCAGAATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1452  AGATCC               [GA]^GATC[CT]
DsaI          191  CCGCGG               C^C[GA][CT]GG
EcoRI        1860  GAATTC               G^AATTC
KspI          191  CCGCGG               CCGC^GG
MfeI         2591  CAATTG               C^AATTG
MflI         1452  AGATCC               [GA]^GATC[CT]
MluNI        2873  TGGCCA               TGG^CCA
MroI         2136  TCCGGA               T^CCGGA
MscI         2873  TGGCCA               TGG^CCA
MunI         2591  CAATTG               C^AATTG
NdeI         1973  CATATG               CA^TATG
PacI         2101  TTAATTAA             TTAAT^TAA
PshBI        2047  ATTAAT               AT^TAAT
Psp1406I     2571  AACGTT               AA^CGTT
SacI         2500  GAGCTC               GAGCT^C
SacII         191  CCGCGG               CCGC^GG
SalI          342  GTCGAC               G^TCGAC
ScaI         2506  AGTACT               AGT^ACT
SmaI          269  CCCGGG               CCC^GGG
SspI         2044  AATATT               AAT^ATT
StuI         1644  AGGCCT               AGG^CCT
XhoII        1452  AGATCC               [GA]^GATC[CT]
XmaCI         269  CCCGGG               C^CCGGG
XmaI          269  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcsI         1860  GAATTC               [GA]^AATT[CT]
             2480  AAATTC              
AcyI          264  GGCGCC               G[GA]^CG[CT]C
             3320  GGCGCC              
AocI          400  CCTGAGG              CC^T[ACGT]AGG
             2377  CCTGAGG             
ApoI         1860  GAATTC               [GA]^AATT[CT]
             2480  AAATTC              
BbeI          264  GGCGCC               GGCGC^C
             3320  GGCGCC              
BclI         2187  TGATCA               T^GATCA
             2621  TGATCA              
BsaBI        2969  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3487  GATCATTATC          
BsaHI         264  GGCGCC               G[GA]^CG[CT]C
             3320  GGCGCC              
BsmFI         273  GGGAC                GGGAC(10/14)
              323  GGGAC               
BsrBI         287  CCGCTC               CCGCTC(-3/-3)
              307  GAGCGG              
Bsu36I        400  CCTGAGG              CC^T[ACGT]AGG
             2377  CCTGAGG             
DraII         273  GGGACCC              [GA]G^G[ACGT]CC[CT]
              323  GGGACCC             
Eco81I        400  CCTGAGG              CC^T[ACGT]AGG
             2377  CCTGAGG             
EcoNI        1441  CCTTTGTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2377  CCTGAGGGAGG         
EcoO109I      273  GGGACCC              [GA]G^G[ACGT]CC[CT]
              323  GGGACCC             
FbaI         2187  TGATCA               T^GATCA
             2621  TGATCA              
HaeII         264  GGCGCC               [GA]GCGC^[CT]
             3320  GGCGCC              
Hin1I         264  GGCGCC               G[GA]^CG[CT]C
             3320  GGCGCC              
KasI          264  GGCGCC               G^GCGCC
             3320  GGCGCC              
MaeII        2264  ACGT                 A^CGT
             2572  ACGT                
MamI         2969  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3487  GATCATTATC          
MslI         1839  CATTGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2686  CACAAAAGTG          
MspA1I        191  CCGCGG               C[AC]G^C[GT]G
              364  CCGCTG              
NarI          264  GGCGCC               GG^CGCC
             3320  GGCGCC              
PpuMI         273  GGGACCC              [GA]G^G[AT]CC[CT]
              323  GGGACCC             
SapI          571  GCTCTTC              GCTCTTC(1/4)
             2056  GCTCTTC             
SfoI          264  GGCGCC               GGC^GCC
             3320  GGCGCC              
SgrAI         219  CGCCGGCG             C[GA]^CCGG[CT]G
              260  CGCCGGCG            
SmlI         1285  CTTGAG               C^T[CT][GA]AG
             2879  CTCAAG              
TaqI          343  TCGA                 T^CGA
             1235  TCGA                
TthHB8I       343  TCGA                 T^CGA
             1235  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsoBI       Bsp1286I    BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       EaeI        EagI        EarI        EclXI       
Eco52I      EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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