Restriction map for pF1KSDA0886
 (Linear) (Six-base) MAPSORT of: KIAA0886.seq  Check: 460  from: 1  to: 3576



KIAA0886.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AccI GT'mk_AC

Cuts at:      0     343    3576 
   Size:        343    3233
 
AclI AA'CG_TT

Cuts at:      0    2572    3576 
   Size:       2572    1004
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1067    1445    1661    1910    2993    3257    3376 
   Size:        803     264     378     216     249    1083     264     119

Cuts at:   3376    3429    3576 
   Size:         53     147

  Fragments arranged by size: 

                1083     803     378     264     264     249     216     147
                 119      53
 
AflIII A'CryG_T

Cuts at:      0    1387    3576 
   Size:       1387    2189
 
AleI CACnn'nnGTG

Cuts at:      0    2690    3576 
   Size:       2690     886
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1803    2758    3576 
   Size:       1803     955     818

  Fragments arranged by size: 

                1803     955     818
 
AlwNI CAG_nnn'CTG

Cuts at:      0     934    1414    2185    3576 
   Size:        934     480     771    1391

  Fragments arranged by size: 

                1391     934     771     480
 
ApoI r'AATT_y

Cuts at:      0    1860    2480    3576 
   Size:       1860     620    1096

  Fragments arranged by size: 

                1860    1096     620
 
AscI GG'CGCG_CC

Cuts at:      0     224    3576 
   Size:        224    3352
 
AseI AT'TA_AT

Cuts at:      0    2048    3576 
   Size:       2048    1528
 
AvaI C'yCGr_G

Cuts at:      0      91     269     327     543    3576 
   Size:         91     178      58     216    3033

  Fragments arranged by size: 

                3033     216     178      91      58
 
AvrII C'CTAG_G

Cuts at:      0     955    3576 
   Size:        955    2621
 
BanI G'GyrC_C

Cuts at:      0     264    3064    3320    3394    3576 
   Size:        264    2800     256      74     182

  Fragments arranged by size: 

                2800     264     256     182      74
 
BanII G_rGCy'C

Cuts at:      0      92     334     457     470     541     550    2504    3576 
   Size:         92     242     123      13      71       9    1954    1072

  Fragments arranged by size: 

                1954    1072     242     123      92      71      13       9
            
 
BbeI G_GCGC'C

Cuts at:      0     268    3324    3576 
   Size:        268    3056     252

  Fragments arranged by size: 

                3056     268     252
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      11     146    1073    3052    3576 
   Size:         11     135     927    1979     524

  Fragments arranged by size: 

                1979     927     524     135      11
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2691    3576 
   Size:       2691     885
 
BclI T'GATC_A

Cuts at:      0    2187    2621    3576 
   Size:       2187     434     955

  Fragments arranged by size: 

                2187     955     434
 
Bme1580I G_kGCm'C

Cuts at:      0     206     353    3576 
   Size:        206     147    3223

  Fragments arranged by size: 

                3223     206     147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     193    1337    3576 
   Size:        193    1144    2239

  Fragments arranged by size: 

                2239    1144     193
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     175     184    1244    1637    2840    3051    3071 
   Size:        166       9       9    1060     393    1203     211      20

Cuts at:   3071    3576 
   Size:        505

  Fragments arranged by size: 

                1203    1060     505     393     211     166      20       9
                   9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1306    2864    3576 
   Size:       1306    1558     712

  Fragments arranged by size: 

                1558    1306     712
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      33     553     831    3576 
   Size:         33     520     278    2745

  Fragments arranged by size: 

                2745     520     278      33
 
BsaBI GATnn'nnATC

Cuts at:      0    2973    3491    3576 
   Size:       2973     518      85

  Fragments arranged by size: 

                2973     518      85
 
BsaHI Gr'CG_yC

Cuts at:      0     265    3321    3576 
   Size:        265    3056     255

  Fragments arranged by size: 

                3056     265     255
 
BsaWI w'CCGG_w

Cuts at:      0    2136    3576 
   Size:       2136    1440
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1377    1407    2058    2088    3576 
   Size:       1377      30     651      30    1488

  Fragments arranged by size: 

                1488    1377     651      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      11     115     127     130     139     163     425     531 
   Size:         11     104      12       3       9      24     262     106

Cuts at:    531     594     707    2512    3268    3576 
   Size:         63     113    1805     756     308

  Fragments arranged by size: 

                1805     756     308     262     113     106     104      63
                  24      12      11       9       3
 
BseYI C'CCAG_C

Cuts at:      0    1404    1900    2719    3576 
   Size:       1404     496     819     857

  Fragments arranged by size: 

                1404     857     819     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3285    3576 
   Size:       3285     291
 
BsiEI CG_ry'CG

Cuts at:      0      55     287     347     521    3576 
   Size:         55     232      60     174    3055

  Fragments arranged by size: 

                3055     232     174      60      55
 
BsiHKAI G_wGCw'C

Cuts at:      0    1395    2504    2846    3576 
   Size:       1395    1109     342     730

  Fragments arranged by size: 

                1395    1109     730     342
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     206     334     353     457     470     541     550 
   Size:         92     114     128      19     104      13      71       9

Cuts at:    550    1395    2504    2846    3576 
   Size:        845    1109     342     730

  Fragments arranged by size: 

                1109     845     730     342     128     114     104      92
                  71      19      13       9
 
BspEI T'CCGG_A

Cuts at:      0    2136    3576 
   Size:       2136    1440
 
BsrBI CCG'CTC

Cuts at:      0     289     309    3576 
   Size:        289      20    3267

  Fragments arranged by size: 

                3267     289      20
 
BsrDI GCAATG_nn'

Cuts at:      0    1421    2725    3125    3576 
   Size:       1421    1304     400     451

  Fragments arranged by size: 

                1421    1304     451     400
 
BsrFI r'CCGG_y

Cuts at:      0     220     261     282    3576 
   Size:        220      41      21    3294

  Fragments arranged by size: 

                3294     220      41      21
 
BssHII G'CGCG_C

Cuts at:      0     224    3576 
   Size:        224    3352
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     934    3576 
   Size:        934    2642
 
BstYI r'GATC_y

Cuts at:      0    1452    3576 
   Size:       1452    2124
 
Bsu36I CC'TnA_GG

Cuts at:      0     401    2378    3576 
   Size:        401    1977    1198

  Fragments arranged by size: 

                1977    1198     401
 
BtgI C'CryG_G

Cuts at:      0     191    3576 
   Size:        191    3385
 
BtsI GCAGTG_nn'

Cuts at:      0    1130    3371    3576 
   Size:       1130    2241     205

  Fragments arranged by size: 

                2241    1130     205
 
EaeI y'GGCC_r

Cuts at:      0      52     284     443     518    2873    3576 
   Size:         52     232     159      75    2355     703

  Fragments arranged by size: 

                2355     703     232     159      75      52
 
EagI C'GGCC_G

Cuts at:      0      52     284     518    3576 
   Size:         52     232     234    3058

  Fragments arranged by size: 

                3058     234     232      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     104     116     578     707     986    1796    1973    2063 
   Size:        104      12     462     129     279     810     177      90

Cuts at:   2063    2780    3331    3448    3576 
   Size:        717     551     117     128

  Fragments arranged by size: 

                 810     717     551     462     279     177     129     128
                 117     104      90      12
 
EcoICRI GAG'CTC

Cuts at:      0    2502    3576 
   Size:       2502    1074
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     175     184     803    1067    1244    1445    1637 
   Size:        166       9       9     619     264     177     201     192

Cuts at:   1637    1661    1910    2840    2993    3051    3071    3257    3376 
   Size:         24     249     930     153      58      20     186     119

Cuts at:   3376    3429    3576 
   Size:         53     147

  Fragments arranged by size: 

                 930     619     264     249     201     192     186     177
                 166     153     147     119      58      53      24      20
                   9       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1445    2381    3576 
   Size:       1445     936    1195

  Fragments arranged by size: 

                1445    1195     936
 
EcoO109I rG'GnC_Cy

Cuts at:      0     274     324    3576 
   Size:        274      50    3252

  Fragments arranged by size: 

                3252     274      50
 
EcoRI G'AATT_C

Cuts at:      0    1860    3576 
   Size:       1860    1716
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     808    2862    3576 
   Size:        808    2054     714

  Fragments arranged by size: 

                2054     808     714
 
HaeII r_GCGC'y

Cuts at:      0     268    3324    3576 
   Size:        268    3056     252

  Fragments arranged by size: 

                3056     268     252
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     658     690    1217    1249    1367    1399    2953    2985 
   Size:        658      32     527      32     118      32    1554      32

Cuts at:   2985    3414    3446    3576 
   Size:        429      32     130

  Fragments arranged by size: 

                1554     658     527     429     130     118      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     344     767    2235    3018    3576 
   Size:        344     423    1468     783     558

  Fragments arranged by size: 

                1468     783     558     423     344
 
HindIII A'AGCT_T

Cuts at:      0     812    1919    2116    3576 
   Size:        812    1107     197    1460

  Fragments arranged by size: 

                1460    1107     812     197
 
KasI G'GCGC_C

Cuts at:      0     264    3320    3576 
   Size:        264    3056     256

  Fragments arranged by size: 

                3056     264     256
 
MfeI C'AATT_G

Cuts at:      0    2591    3576 
   Size:       2591     985
 
MscI TGG'CCA

Cuts at:      0    2875    3576 
   Size:       2875     701
 
MslI CAynn'nnrTG

Cuts at:      0    1843    2690    3576 
   Size:       1843     847     886

  Fragments arranged by size: 

                1843     886     847
 
MspA1I CmG'CkG

Cuts at:      0     193     366    3576 
   Size:        193     173    3210

  Fragments arranged by size: 

                3210     193     173
 
NaeI GCC'GGC

Cuts at:      0     222     263     284    3576 
   Size:        222      41      21    3292

  Fragments arranged by size: 

                3292     222      41      21
 
NarI GG'CG_CC

Cuts at:      0     265    3321    3576 
   Size:        265    3056     255

  Fragments arranged by size: 

                3056     265     255
 
NdeI CA'TA_TG

Cuts at:      0    1974    3576 
   Size:       1974    1602
 
NgoMIV G'CCGG_C

Cuts at:      0     220     261     282    3576 
   Size:        220      41      21    3294

  Fragments arranged by size: 

                3294     220      41      21
 
NspI r_CATG'y

Cuts at:      0    1391    1638    1676    2096    3576 
   Size:       1391     247      38     420    1480

  Fragments arranged by size: 

                1480    1391     420     247      38
 
PacI TTA_AT'TAA

Cuts at:      0    2105    3576 
   Size:       2105    1471
 
PciI A'CATG_T

Cuts at:      0    1387    3576 
   Size:       1387    2189
 
PpuMI rG'GwC_Cy

Cuts at:      0     274     324    3576 
   Size:        274      50    3252

  Fragments arranged by size: 

                3252     274      50
 
PstI C_TGCA'G

Cuts at:      0     612    1779    1869    2514    3576 
   Size:        612    1167      90     645    1062

  Fragments arranged by size: 

                1167    1062     645     612      90
 
SacI G_AGCT'C

Cuts at:      0    2504    3576 
   Size:       2504    1072
 
SacII CC_GC'GG

Cuts at:      0     194    3576 
   Size:        194    3382
 
SalI G'TCGA_C

Cuts at:      0     342    3576 
   Size:        342    3234
 
SanDI GG'GwC_CC

Cuts at:      0     274     324    3576 
   Size:        274      50    3252

  Fragments arranged by size: 

                3252     274      50
 
SapI GCTCTTCn'nnn_

Cuts at:      0     578    2063    3576 
   Size:        578    1485    1513

  Fragments arranged by size: 

                1513    1485     578
 
ScaI AGT'ACT

Cuts at:      0    2508    3576 
   Size:       2508    1068
 
SfcI C'TryA_G

Cuts at:      0     608    1040    1775    1865    2510    3576 
   Size:        608     432     735      90     645    1066

  Fragments arranged by size: 

                1066     735     645     608     432      90
 
SfoI GGC'GCC

Cuts at:      0     266    3322    3576 
   Size:        266    3056     254

  Fragments arranged by size: 

                3056     266     254
 
SgrAI Cr'CCGG_yG

Cuts at:      0     220     261    3576 
   Size:        220      41    3315

  Fragments arranged by size: 

                3315     220      41
 
SmaI CCC'GGG

Cuts at:      0     271    3576 
   Size:        271    3305
 
SmlI C'TyrA_G

Cuts at:      0    1285    2879    3576 
   Size:       1285    1594     697

  Fragments arranged by size: 

                1594    1285     697
 
SspI AAT'ATT

Cuts at:      0    2046    3576 
   Size:       2046    1530
 
StuI AGG'CCT

Cuts at:      0    1646    3576 
   Size:       1646    1930
 
StyI C'CwwG_G

Cuts at:      0     753     955    3131    3576 
   Size:        753     202    2176     445

  Fragments arranged by size: 

                2176     753     445     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1489    3227    3576 
   Size:       1489    1738     349

  Fragments arranged by size: 

                1738    1489     349
 
TatI w'GTAC_w

Cuts at:      0    1662    2506    3026    3269    3576 
   Size:       1662     844     520     243     307

  Fragments arranged by size: 

                1662     844     520     307     243
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1377    1409    3576 
   Size:       1377      32    2167

  Fragments arranged by size: 

                2167    1377      32
 
XmaI C'CCGG_G

Cuts at:      0     269    3576 
   Size:        269    3307
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI
     AscI     AseI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BciVI     BclI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII
     KasI     MfeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NspI     PacI     PciI    PpuMI     PstI     SacI    SacII
     SalI    SanDI     SapI     ScaI     SfcI     SfoI    SgrAI     SmaI
     SmlI     SspI     StuI     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AloI
     ApaI    ApaLI    AsiSI     BaeI    BamHI    BbvCI     BcgI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrGI    BssSI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI    EcoRV     FseI     FspI    FspAI     HpaI     KpnI
     MluI     MmeI     NcoI     NheI     NotI     NruI     NsiI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SbfI    SexAI     SfiI
     SgfI    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI
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