Restriction map for pF1KSDA0875
 (Linear) (Six-base) MAPSORT of: KIAA0875.seq  Check: 5130  from: 1  to: 1863



KIAA0875.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     181     727    1564    1863 
   Size:        181     546     837     299

  Fragments arranged by size: 

                 837     546     299     181
 
AccI GT'mk_AC

Cuts at:      0      17    1863 
   Size:         17    1846
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     375     541     838    1863 
   Size:        375     166     297    1025

  Fragments arranged by size: 

                1025     375     297     166
 
AflII C'TTAA_G

Cuts at:      0     544    1863 
   Size:        544    1319
 
AhdI GACnn_n'nnGTC

Cuts at:      0     576    1863 
   Size:        576    1287
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     931    1130    1863 
   Size:        931     199     733

  Fragments arranged by size: 

                 931     733     199
 
AlwNI CAG_nnn'CTG

Cuts at:      0     173     783    1221    1863 
   Size:        173     610     438     642

  Fragments arranged by size: 

                 642     610     438     173
 
ApoI r'AATT_y

Cuts at:      0     503    1863 
   Size:        503    1360
 
AvaI C'yCGr_G

Cuts at:      0    1362    1863 
   Size:       1362     501
 
BamHI G'GATC_C

Cuts at:      0    1577    1863 
   Size:       1577     286
 
BanI G'GyrC_C

Cuts at:      0      36     995    1863 
   Size:         36     959     868

  Fragments arranged by size: 

                 959     868      36
 
BanII G_rGCy'C

Cuts at:      0    1499    1863 
   Size:       1499     364
 
BbvCI CC'TCA_GC

Cuts at:      0      79    1863 
   Size:         79    1784
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     460    1799    1863 
   Size:        460    1339      64

  Fragments arranged by size: 

                1339     460      64
 
BfrBI ATG'CAT

Cuts at:      0     870    1863 
   Size:        870     993
 
BglI GCCn_nnn'nGGC

Cuts at:      0     733    1863 
   Size:        733    1130
 
BglII A'GATC_T

Cuts at:      0     530    1797    1863 
   Size:        530    1267      66

  Fragments arranged by size: 

                1267     530      66
 
BmgBI CAC'GTC

Cuts at:      0    1722    1863 
   Size:       1722     141
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1668    1863 
   Size:       1668     195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     150     754     976    1324    1798    1822    1863 
   Size:        133      17     604     222     348     474      24      41

  Fragments arranged by size: 

                 604     474     348     222     133      41      24      17
            
 
Bpu10I CC'TnA_GC

Cuts at:      0      79    1863 
   Size:         79    1784
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1685    1863 
   Size:       1685     178
 
BsaAI yAC'GTr

Cuts at:      0    1110    1863 
   Size:       1110     753
 
BsaBI GATnn'nnATC

Cuts at:      0    1241    1863 
   Size:       1241     622
 
BsaWI w'CCGG_w

Cuts at:      0    1580    1784    1863 
   Size:       1580     204      79

  Fragments arranged by size: 

                1580     204      79
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1771    1801    1863 
   Size:       1771      30      62

  Fragments arranged by size: 

                1771      62      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67    1267    1270    1444    1863 
   Size:         67    1200       3     174     419

  Fragments arranged by size: 

                1200     419     174      67       3
 
BseYI C'CCAG_C

Cuts at:      0     316     718    1544    1863 
   Size:        316     402     826     319

  Fragments arranged by size: 

                 826     402     319     316
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1288    1414    1843    1863 
   Size:       1288     126     429      20

  Fragments arranged by size: 

                1288     429     126      20
 
BsiEI CG_ry'CG

Cuts at:      0     146     159    1863 
   Size:        146      13    1704

  Fragments arranged by size: 

                1704     146      13
 
BsiHKAI G_wGCw'C

Cuts at:      0     374    1412    1863 
   Size:        374    1038     451

  Fragments arranged by size: 

                1038     451     374
 
BsmI GAATG_Cn'

Cuts at:      0    1090    1863 
   Size:       1090     773
 
Bsp1286I G_dGCh'C

Cuts at:      0     374    1412    1499    1863 
   Size:        374    1038      87     364

  Fragments arranged by size: 

                1038     374     364      87
 
BspEI T'CCGG_A

Cuts at:      0    1580    1863 
   Size:       1580     283
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     104     181     524     727    1564    1863 
   Size:        104      77     343     203     837     299

  Fragments arranged by size: 

                 837     343     299     203     104      77
 
BsrDI GCAATG_nn'

Cuts at:      0    1256    1863 
   Size:       1256     607
 
BsrFI r'CCGG_y

Cuts at:      0      39     176    1863 
   Size:         39     137    1687

  Fragments arranged by size: 

                1687     137      39
 
BssSI C'ACGA_G

Cuts at:      0    1570    1863 
   Size:       1570     293
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     756    1863 
   Size:        756    1107
 
BstYI r'GATC_y

Cuts at:      0     530    1236    1577    1781    1797    1863 
   Size:        530     706     341     204      16      66

  Fragments arranged by size: 

                 706     530     341     204      66      16
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      40    1863 
   Size:         40    1823
 
EaeI y'GGCC_r

Cuts at:      0     143     156    1101    1315    1611    1863 
   Size:        143      13     945     214     296     252

  Fragments arranged by size: 

                 945     296     252     214     143      13
 
EagI C'GGCC_G

Cuts at:      0     143     156    1863 
   Size:        143      13    1707

  Fragments arranged by size: 

                1707     143      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     287    1863 
   Size:        287    1576
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      64    1863 
   Size:         64    1799
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     150     375     541     754     838     976    1324 
   Size:        133      17     225     166     213      84     138     348

Cuts at:   1324    1798    1822    1863 
   Size:        474      24      41

  Fragments arranged by size: 

                 474     348     225     213     166     138     133      84
                  41      24      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1548    1863 
   Size:       1548     315
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     378     491    1309    1863 
   Size:        378     113     818     554

  Fragments arranged by size: 

                 818     554     378     113
 
HaeII r_GCGC'y

Cuts at:      0      46    1863 
   Size:         46    1817
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     562     594     918     950    1773    1805    1863 
   Size:        562      32     324      32     823      32      58

  Fragments arranged by size: 

                 823     562     324      58      32      32      32
 
HincII GTy'rAC

Cuts at:      0      18      93     966    1863 
   Size:         18      75     873     897

  Fragments arranged by size: 

                 897     873      75      18
 
HindIII A'AGCT_T

Cuts at:      0     473    1863 
   Size:        473    1390
 
MfeI C'AATT_G

Cuts at:      0     282     929    1863 
   Size:        282     647     934

  Fragments arranged by size: 

                 934     647     282
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     653    1863 
   Size:        269     384    1210

  Fragments arranged by size: 

                1210     384     269
 
MscI TGG'CCA

Cuts at:      0    1317    1863 
   Size:       1317     546
 
MslI CAynn'nnrTG

Cuts at:      0     754    1475    1558    1863 
   Size:        754     721      83     305

  Fragments arranged by size: 

                 754     721     305      83
 
MspA1I CmG'CkG

Cuts at:      0      83     206    1071    1863 
   Size:         83     123     865     792

  Fragments arranged by size: 

                 865     792     123      83
 
NaeI GCC'GGC

Cuts at:      0      41     178    1863 
   Size:         41     137    1685

  Fragments arranged by size: 

                1685     137      41
 
NgoMIV G'CCGG_C

Cuts at:      0      39     176    1863 
   Size:         39     137    1687

  Fragments arranged by size: 

                1687     137      39
 
NsiI A_TGCA'T

Cuts at:      0     872    1863 
   Size:        872     991
 
NspI r_CATG'y

Cuts at:      0     912    1863 
   Size:        912     951
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     783    1863 
   Size:        783    1080
 
PmlI CAC'GTG

Cuts at:      0    1110    1863 
   Size:       1110     753
 
PpuMI rG'GwC_Cy

Cuts at:      0    1548    1863 
   Size:       1548     315
 
PstI C_TGCA'G

Cuts at:      0    1119    1863 
   Size:       1119     744
 
PvuII CAG'CTG

Cuts at:      0      83    1071    1863 
   Size:         83     988     792

  Fragments arranged by size: 

                 988     792      83
 
SalI G'TCGA_C

Cuts at:      0      16    1863 
   Size:         16    1847
 
SanDI GG'GwC_CC

Cuts at:      0    1548    1863 
   Size:       1548     315
 
ScaI AGT'ACT

Cuts at:      0     613    1093    1863 
   Size:        613     480     770

  Fragments arranged by size: 

                 770     613     480
 
SexAI A'CCwGG_T

Cuts at:      0    1262    1388    1863 
   Size:       1262     126     475

  Fragments arranged by size: 

                1262     475     126
 
SfcI C'TryA_G

Cuts at:      0     261    1115    1863 
   Size:        261     854     748

  Fragments arranged by size: 

                 854     748     261
 
SmaI CCC'GGG

Cuts at:      0    1364    1863 
   Size:       1364     499
 
SmlI C'TyrA_G

Cuts at:      0     544    1700    1863 
   Size:        544    1156     163

  Fragments arranged by size: 

                1156     544     163
 
SspI AAT'ATT

Cuts at:      0    1830    1863 
   Size:       1830      33
 
StuI AGG'CCT

Cuts at:      0     550    1863 
   Size:        550    1313
 
StyI C'CwwG_G

Cuts at:      0     999    1863 
   Size:        999     864
 
TatI w'GTAC_w

Cuts at:      0     116     611    1091    1863 
   Size:        116     495     480     772

  Fragments arranged by size: 

                 772     495     480     116
 
Tth111I GACn'n_nGTC

Cuts at:      0     277    1863 
   Size:        277    1586
 
XbaI T'CTAG_A

Cuts at:      0    1404    1863 
   Size:       1404     459
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1560    1863 
   Size:       1560     303
 
XmaI C'CCGG_G

Cuts at:      0    1362    1863 
   Size:       1362     501
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AhdI     AlfI    AlwNI     ApoI
     AvaI    BamHI     BanI    BanII    BbvCI    BciVI    BfrBI     BglI
    BglII    BmgBI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BssSI    BstXI    BstYI
    BtgZI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     NsiI     NspI    PflMI     PmlI    PpuMI
     PstI    PvuII     SalI    SanDI     ScaI    SexAI     SfcI     SmaI
     SmlI     SspI     StuI     StyI     TatI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BbsI     BcgI     BclI     BlpI Bme1580I     BmrI     BmtI     BsaI
    BsaHI    BsiWI    BsmBI    BspHI    BsrBI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MluI     NarI     NcoI
     NdeI     NheI     NotI     NruI     PacI     PasI     PciI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SapI     SbfI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TstI
     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com