Restriction map for pF1KSDA0854
 (Linear) (Six-base) MAPSORT of: KIAA0854.seq  Check: 7138  from: 1  to: 2511



KIAA0854.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AccI GT'mk_AC

Cuts at:      0      80     290    2511 
   Size:         80     210    2221

  Fragments arranged by size: 

                2221     210      80
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442    2511 
   Size:        442    2069
 
AfeI AGC'GCT

Cuts at:      0     940    2511 
   Size:        940    1571
 
AflIII A'CryG_T

Cuts at:      0     329     720    2511 
   Size:        329     391    1791

  Fragments arranged by size: 

                1791     391     329
 
AgeI A'CCGG_T

Cuts at:      0    2309    2511 
   Size:       2309     202
 
AleI CACnn'nnGTG

Cuts at:      0    1066    2511 
   Size:       1066    1445
 
AlwNI CAG_nnn'CTG

Cuts at:      0     923    2500    2511 
   Size:        923    1577      11

  Fragments arranged by size: 

                1577     923      11
 
AvaI C'yCGr_G

Cuts at:      0      70     400     607     622    2511 
   Size:         70     330     207      15    1889

  Fragments arranged by size: 

                1889     330     207      70      15
 
BanI G'GyrC_C

Cuts at:      0     594    1006    1275    1855    2511 
   Size:        594     412     269     580     656

  Fragments arranged by size: 

                 656     594     580     412     269
 
BanII G_rGCy'C

Cuts at:      0     701    1286    1818    2158    2511 
   Size:        701     585     532     340     353

  Fragments arranged by size: 

                 701     585     532     353     340
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1342    1640    2511 
   Size:       1342     298     871

  Fragments arranged by size: 

                1342     871     298
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1498    1532    2511 
   Size:       1498      34     979

  Fragments arranged by size: 

                1498     979      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     807    2511 
   Size:        807    1704
 
BclI T'GATC_A

Cuts at:      0     830    2103    2511 
   Size:        830    1273     408

  Fragments arranged by size: 

                1273     830     408
 
BfrBI ATG'CAT

Cuts at:      0      32    2511 
   Size:         32    2479
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1054    1540    2333    2511 
   Size:       1054     486     793     178

  Fragments arranged by size: 

                1054     793     486     178
 
BlpI GC'TnA_GC

Cuts at:      0    1702    2511 
   Size:       1702     809
 
Bme1580I G_kGCm'C

Cuts at:      0      71     599     767    1860    2511 
   Size:         71     528     168    1093     651

  Fragments arranged by size: 

                1093     651     528     168      71
 
BmgBI CAC'GTC

Cuts at:      0     123     723    2511 
   Size:        123     600    1788

  Fragments arranged by size: 

                1788     600     123
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1044    1854    1944    2511 
   Size:       1044     810      90     567

  Fragments arranged by size: 

                1044     810     567      90
 
BmtI G_CTAG'C

Cuts at:      0       8    2511 
   Size:          8    2503
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     693    1737    2319    2511 
   Size:        693    1044     582     192

  Fragments arranged by size: 

                1044     693     582     192
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1069    1360    2451    2511 
   Size:       1069     291    1091      60

  Fragments arranged by size: 

                1091    1069     291      60
 
Bpu10I CC'TnA_GC

Cuts at:      0    1271    1684    2511 
   Size:       1271     413     827

  Fragments arranged by size: 

                1271     827     413
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     663    1345    2511 
   Size:        663     682    1166

  Fragments arranged by size: 

                1166     682     663
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1212    1687    2511 
   Size:       1212     475     824

  Fragments arranged by size: 

                1212     824     475
 
BsaAI yAC'GTr

Cuts at:      0     330     633    1562    2379    2511 
   Size:        330     303     929     817     132

  Fragments arranged by size: 

                 929     817     330     303     132
 
BsaWI w'CCGG_w

Cuts at:      0    1852    2309    2511 
   Size:       1852     457     202

  Fragments arranged by size: 

                1852     457     202
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     370     400    1834    1864    2511 
   Size:        370      30    1434      30     647

  Fragments arranged by size: 

                1434     647     370      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     625     691     922     994    1944    2371    2413    2511 
   Size:        625      66     231      72     950     427      42      98

  Fragments arranged by size: 

                 950     625     427     231      98      72      66      42
            
 
BseYI C'CCAG_C

Cuts at:      0     935    1879    2511 
   Size:        935     944     632

  Fragments arranged by size: 

                 944     935     632
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356    2511 
   Size:        356    2155
 
BsiEI CG_ry'CG

Cuts at:      0    1557    2393    2511 
   Size:       1557     836     118

  Fragments arranged by size: 

                1557     836     118
 
BsiHKAI G_wGCw'C

Cuts at:      0     701     914    1830    1936    2511 
   Size:        701     213     916     106     575

  Fragments arranged by size: 

                 916     701     575     213     106
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1167    2511 
   Size:       1167    1344
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     599     701     767     914    1286    1818    1830 
   Size:         71     528     102      66     147     372     532      12

Cuts at:   1830    1860    1936    2158    2511 
   Size:         30      76     222     353

  Fragments arranged by size: 

                 532     528     372     353     222     147     102      76
                  71      66      30      12
 
BspHI T'CATG_A

Cuts at:      0     557    2511 
   Size:        557    1954
 
BsrBI CCG'CTC

Cuts at:      0    1311    2511 
   Size:       1311    1200
 
BsrFI r'CCGG_y

Cuts at:      0    1409    2309    2511 
   Size:       1409     900     202

  Fragments arranged by size: 

                1409     900     202
 
BsrGI T'GTAC_A

Cuts at:      0     735    2511 
   Size:        735    1776
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     245    2511 
   Size:        245    2266
 
BstEII G'GTnAC_C

Cuts at:      0    1140    2235    2511 
   Size:       1140    1095     276

  Fragments arranged by size: 

                1140    1095     276
 
BstYI r'GATC_y

Cuts at:      0     533     671    1106    2511 
   Size:        533     138     435    1405

  Fragments arranged by size: 

                1405     533     435     138
 
Bsu36I CC'TnA_GG

Cuts at:      0    1927    2511 
   Size:       1927     584
 
BtgI C'CryG_G

Cuts at:      0    1187    1206    1781    2093    2144    2511 
   Size:       1187      19     575     312      51     367

  Fragments arranged by size: 

                1187     575     367     312      51      19
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1251    1521    2511 
   Size:       1251     270     990

  Fragments arranged by size: 

                1251     990     270
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1149    2511 
   Size:       1149    1362
 
EaeI y'GGCC_r

Cuts at:      0     125    1113    1209    1298    1532    1985    2147    2334 
   Size:        125     988      96      89     234     453     162     187

Cuts at:   2334    2502    2511 
   Size:        168       9

  Fragments arranged by size: 

                 988     453     234     187     168     162     125      96
                  89       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     165     965    1210    2210    2511 
   Size:        165     800     245    1000     301

  Fragments arranged by size: 

                1000     800     301     245     165
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1755    2511 
   Size:       1755     756
 
EcoICRI GAG'CTC

Cuts at:      0     699    2511 
   Size:        699    1812
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442    1069    1360    2451    2511 
   Size:        442     627     291    1091      60

  Fragments arranged by size: 

                1091     627     442     291      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     771    2196    2511 
   Size:        771    1425     315

  Fragments arranged by size: 

                1425     771     315
 
EcoRV GAT'ATC

Cuts at:      0    1474    2511 
   Size:       1474    1037
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1239    1386    2511 
   Size:       1239     147    1125

  Fragments arranged by size: 

                1239    1125     147
 
HaeII r_GCGC'y

Cuts at:      0     942    2511 
   Size:        942    1569
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     175     207     358     370     390     402    1341    1373 
   Size:        175      32     151      12      20      12     939      32

Cuts at:   1373    1834    1866    2395    2427    2511 
   Size:        461      32     529      32      84

  Fragments arranged by size: 

                 939     529     461     175     151      84      32      32
                  32      32      20      12      12
 
HincII GTy'rAC

Cuts at:      0     291    1154    1865    2511 
   Size:        291     863     711     646

  Fragments arranged by size: 

                 863     711     646     291
 
HindIII A'AGCT_T

Cuts at:      0    1744    2511 
   Size:       1744     767
 
HpaI GTT'AAC

Cuts at:      0    1865    2511 
   Size:       1865     646
 
MfeI C'AATT_G

Cuts at:      0    1895    2511 
   Size:       1895     616
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     401    2438    2504    2511 
   Size:        401    2037      66       7

  Fragments arranged by size: 

                2037     401      66       7
 
MscI TGG'CCA

Cuts at:      0     127    1211    1300    1534    2504    2511 
   Size:        127    1084      89     234     970       7

  Fragments arranged by size: 

                1084     970     234     127      89       7
 
MslI CAynn'nnrTG

Cuts at:      0    1036    1066    1420    2511 
   Size:       1036      30     354    1091

  Fragments arranged by size: 

                1091    1036     354      30
 
MspA1I CmG'CkG

Cuts at:      0     667     741     889     959    1148    2511 
   Size:        667      74     148      70     189    1363

  Fragments arranged by size: 

                1363     667     189     148      74      70
 
NcoI C'CATG_G

Cuts at:      0    1206    1781    2093    2144    2511 
   Size:       1206     575     312      51     367

  Fragments arranged by size: 

                1206     575     367     312      51
 
NheI G'CTAG_C

Cuts at:      0       4    2511 
   Size:          4    2507
 
NsiI A_TGCA'T

Cuts at:      0      34    2511 
   Size:         34    2477
 
NspI r_CATG'y

Cuts at:      0     289     297    2511 
   Size:        289       8    2214

  Fragments arranged by size: 

                2214     289       8
 
PacI TTA_AT'TAA

Cuts at:      0     431    2511 
   Size:        431    2080
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1809    2094    2511 
   Size:       1809     285     417

  Fragments arranged by size: 

                1809     417     285
 
PmlI CAC'GTG

Cuts at:      0     633    2511 
   Size:        633    1878
 
PpuMI rG'GwC_Cy

Cuts at:      0     771    2196    2511 
   Size:        771    1425     315

  Fragments arranged by size: 

                1425     771     315
 
PstI C_TGCA'G

Cuts at:      0    2496    2511 
   Size:       2496      15
 
PvuII CAG'CTG

Cuts at:      0     667     741     889     959    2511 
   Size:        667      74     148      70    1552

  Fragments arranged by size: 

                1552     667     148      74      70
 
SacI G_AGCT'C

Cuts at:      0     701    2511 
   Size:        701    1810
 
SalI G'TCGA_C

Cuts at:      0     289    2511 
   Size:        289    2222
 
SfcI C'TryA_G

Cuts at:      0    2492    2511 
   Size:       2492      19
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1540    2511 
   Size:       1540     971
 
SmaI CCC'GGG

Cuts at:      0     402    2511 
   Size:        402    2109
 
SmlI C'TyrA_G

Cuts at:      0     678    1360    2511 
   Size:        678     682    1151

  Fragments arranged by size: 

                1151     682     678
 
SspI AAT'ATT

Cuts at:      0    2188    2511 
   Size:       2188     323
 
StyI C'CwwG_G

Cuts at:      0     128     222     767    1206    1223    1292    1781    2093 
   Size:        128      94     545     439      17      69     489     312

Cuts at:   2093    2144    2511 
   Size:         51     367

  Fragments arranged by size: 

                 545     489     439     367     312     128      94      69
                  51      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     917     949    1305    2511 
   Size:        917      32     356    1206

  Fragments arranged by size: 

                1206     917     356      32
 
TatI w'GTAC_w

Cuts at:      0     735    2511 
   Size:        735    1776
 
Tth111I GACn'n_nGTC

Cuts at:      0    1553    2485    2511 
   Size:       1553     932      26

  Fragments arranged by size: 

                1553     932      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1527    1995    2511 
   Size:       1527     468     516

  Fragments arranged by size: 

                1527     516     468
 
XmaI C'CCGG_G

Cuts at:      0     400    2511 
   Size:        400    2111
 
XmnI GAAnn'nnTTC

Cuts at:      0     274     997    1456    1916    2511 
   Size:        274     723     459     460     595

  Fragments arranged by size: 

                 723     595     460     459     274
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AgeI     AleI    AlwNI     AvaI
     BanI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI    BsrGI
   BstAPI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    HaeII
    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     NheI     NsiI     NspI     PacI    PflMI     PmlI
    PpuMI     PstI    PvuII     SacI     SalI     SfcI     SfiI     SmaI
     SmlI     SspI     StyI    TaqII     TatI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AhdI     AlfI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI    BglII    BsaBI    BsaHI    BsiWI     BsmI
    BspEI    BspMI    BsrDI   BssHII    BssSI    BstBI    BstXI  BstZ17I
     BtsI     ClaI    CspCI     DraI   DraIII     EagI    EcoNI    EcoRI
     FseI     FspI    FspAI     KasI     KpnI     MluI     NaeI     NarI
     NdeI   NgoMIV     NotI     NruI     PasI     PciI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
     XbaI     XhoI     ZraI
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