Restriction map for pF1KSDA0851
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AlwNI       
AocI        Aor51HI     ApaI        AscI        Asp718I     
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BssHII      BssSI       BstAPI      BstBI       
BstUI       BstYI       Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRV       
FbaI        FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HincII      HindII      HpaI        
HpaII       KasI        KpnI        KspI        MflI        
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    StuI        SwaI        Tth111I     XcmI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          176  GTATAC               GT^[AC][GT]AC
AhdI          100  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        730  GTGCAC               G^TGCAC
ApaLI         730  GTGCAC               G^TGCAC
AseI          601  ATTAAT               AT^TAAT
AsnI          601  ATTAAT               AT^TAAT
AspEI         100  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1122  TGGCCA               TGG^CCA
BanI         1357  GGTGCC               G^G[CT][GA]CC
BanII         933  GGGCTC               G[GA]GC[CT]^C
BcgI         1156  CGAAGCAATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI         624  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          530  CTGCAC               GTGCAG(16/14)
BsmI          588  GCATTC               GAATGC(1/-1)
Bsp1407I      759  TGTACA               T^GTACA
BspMI         202  GCAGGT               ACCTGC(4/8)
BsrDI        1005  CATTGC               GCAATG(2/0)
BsrGI         759  TGTACA               T^GTACA
Bst1107I      176  GTATAC               GTA^TAC
BstEII        117  GGTTACC              G^GT[ACGT]ACC
BstPI         117  GGTTACC              G^GT[ACGT]ACC
BstXI        1546  CCAAGGGACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       176  GTATAC               GTA^TAC
CfoI          663  GCGC                 GCG^C
EaeI         1122  TGGCCA               [CT]^GGCC[GA]
Eam1105I      100  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1660  CTCTTC               CTCTTC(1/4)
EcoO65I       117  GGTTACC              G^GT[ACGT]ACC
EcoRI         454  GAATTC               G^AATTC
EcoT22I       586  ATGCAT               ATGCA^T
FauI         1348  GCGGG                CCCGC(4/6)
HhaI          663  GCGC                 GCG^C
HinP1I        663  GCGC                 G^CGC
Ksp632I      1660  CTCTTC               CTCTTC(1/4)
MaeII          86  ACGT                 A^CGT
MamI          624  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1161  CAATTG               C^AATTG
MluNI        1122  TGGCCA               TGG^CCA
MscI         1122  TGGCCA               TGG^CCA
MslI          733  CACTACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1161  CAATTG               C^AATTG
NdeI          407  CATATG               CA^TATG
NsiI          586  ATGCAT               ATGCA^T
NspI         1254  GCATGT               [GA]CATG^[CT]
PshBI         601  ATTAAT               AT^TAAT
SapI         1659  GCTCTTC              GCTCTTC(1/4)
SpeI          445  ACTAGT               A^CTAGT
SspBI         759  TGTACA               T^GTACA
SspI          168  AATATT               AAT^ATT
XbaI         1176  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Asp700I       680  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1499  GAAACTATTC          
AspHI         730  GTGCAC               G[AT]GC[AT]^C
             1657  GTGCTC              
AvaI          633  CTCGAG               C^[CT]CG[GA]G
              767  CTCGAG              
AvaII         791  GGTCC                G^G[AT]CC
             1740  GGTCC               
BseRI         636  GAGGAG               GAGGAG(10/8)
              639  GAGGAG              
BsiHKAI       730  GTGCAC               G[AT]GC[AT]^C
             1657  GTGCTC              
BsmAI        1239  GAGAC                GTCTC(1/5)
              470  GTCTC               
BsoBI         633  CTCGAG               C^[CT]CG[GA]G
              767  CTCGAG              
Cac8I        1120  GCTGGC               GC[ACGT]^[ACGT]GC
             1675  GCAAGC              
DdeI          517  CTAAG                C^T[ACGT]AG
             1054  CTAAG               
EcoT14I       980  CCTTGG               C^C[AT][AT]GG
             1546  CCAAGG              
HphI          244  GGTGA                GGTGA(8/7)
             1627  GGTGA               
NlaIV         791  GGTCCC               GG[ACGT]^[ACGT]CC
             1357  GGTGCC              
PaeR7I        633  CTCGAG               C^TCGAG
              767  CTCGAG              
PvuII          22  CAGCTG               CAG^CTG
              152  CAGCTG              
SfaNI         360  GATGC                GCATC(5/9)
             1726  GATGC               
StyI          980  CCTTGG               C^C[AT][AT]GG
             1546  CCAAGG              
Tsp45I        157  GTCAC                ^GT[GC]AC
             1628  GTGAC               
XhoI          633  CTCGAG               C^TCGAG
              767  CTCGAG              
XmnI          680  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1499  GAAACTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HindIII     HinfI       ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
PflMI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     Van91I      
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