Restriction map for pF1KSDA0851
 (Linear) (Six-base) MAPSORT of: KIAA0851.seq  Check: 3023  from: 1  to: 1761



KIAA0851.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AccI GT'mk_AC

Cuts at:      0     177    1761 
   Size:        177    1584
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     134    1207    1219    1761 
   Size:         46      88    1073      12     542

  Fragments arranged by size: 

                1073     542      88      46      12
 
AhdI GACnn_n'nnGTC

Cuts at:      0     105    1761 
   Size:        105    1656
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     977    1761 
   Size:        977     784
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1135    1167    1441    1473    1761 
   Size:       1135      32     274      32     288

  Fragments arranged by size: 

                1135     288     274      32      32
 
ApaLI G'TGCA_C

Cuts at:      0     730    1761 
   Size:        730    1031
 
ApoI r'AATT_y

Cuts at:      0      49     247     454    1285    1558    1761 
   Size:         49     198     207     831     273     203

  Fragments arranged by size: 

                 831     273     207     203     198      49
 
AseI AT'TA_AT

Cuts at:      0     602    1761 
   Size:        602    1159
 
AvaI C'yCGr_G

Cuts at:      0     633     767    1761 
   Size:        633     134     994

  Fragments arranged by size: 

                 994     633     134
 
BanI G'GyrC_C

Cuts at:      0    1357    1761 
   Size:       1357     404
 
BanII G_rGCy'C

Cuts at:      0     937    1761 
   Size:        937     824
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     310     968    1372    1761 
   Size:        310     658     404     389

  Fragments arranged by size: 

                 658     404     389     310
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1145    1179    1761 
   Size:       1145      34     582

  Fragments arranged by size: 

                1145     582      34
 
BfrBI ATG'CAT

Cuts at:      0     588    1761 
   Size:        588    1173
 
Bme1580I G_kGCm'C

Cuts at:      0     187     734    1761 
   Size:        187     547    1027

  Fragments arranged by size: 

                1027     547     187
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     190    1761 
   Size:        190    1571
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793     970    1761 
   Size:        793     177     791

  Fragments arranged by size: 

                 793     791     177
 
BsaBI GATnn'nnATC

Cuts at:      0     628    1761 
   Size:        628    1133
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     630     660    1761 
   Size:        630      30    1101

  Fragments arranged by size: 

                1101     630      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     651     654    1761 
   Size:        651       3    1107

  Fragments arranged by size: 

                1107     651       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1761 
   Size:        515    1246
 
BsiHKAI G_wGCw'C

Cuts at:      0     734    1661    1761 
   Size:        734     927     100

  Fragments arranged by size: 

                 927     734     100
 
BsmI GAATG_Cn'

Cuts at:      0     588    1761 
   Size:        588    1173
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     734     937    1661    1761 
   Size:        187     547     203     724     100

  Fragments arranged by size: 

                 724     547     203     187     100
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     193    1761 
   Size:        193    1568
 
BsrDI GCAATG_nn'

Cuts at:      0    1004    1761 
   Size:       1004     757
 
BsrGI T'GTAC_A

Cuts at:      0     759    1761 
   Size:        759    1002
 
BstEII G'GTnAC_C

Cuts at:      0     117    1761 
   Size:        117    1644
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1553    1761 
   Size:       1553     208
 
BstZ17I GTA'TAC

Cuts at:      0     178    1761 
   Size:        178    1583
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     373    1761 
   Size:        373    1388
 
BtsI GCAGTG_nn'

Cuts at:      0     820    1761 
   Size:        820     941
 
EaeI y'GGCC_r

Cuts at:      0    1122    1761 
   Size:       1122     639
 
EarI CTCTTCn'nnn_

Cuts at:      0    1666    1761 
   Size:       1666      95
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     134    1207    1219    1761 
   Size:         46      88    1073      12     542

  Fragments arranged by size: 

                1073     542      88      46      12
 
EcoRI G'AATT_C

Cuts at:      0     454    1761 
   Size:        454    1307
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1389    1470    1761 
   Size:       1389      81     291

  Fragments arranged by size: 

                1389     291      81
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     791     823    1032    1064    1761 
   Size:         91      32     668      32     209      32     697

  Fragments arranged by size: 

                 697     668     209      91      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0      62     749    1267    1761 
   Size:         62     687     518     494

  Fragments arranged by size: 

                 687     518     494      62
 
MfeI C'AATT_G

Cuts at:      0    1161    1761 
   Size:       1161     600
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1761 
   Size:        464    1297
 
MscI TGG'CCA

Cuts at:      0    1124    1761 
   Size:       1124     637
 
MslI CAynn'nnrTG

Cuts at:      0     737    1761 
   Size:        737    1024
 
MspA1I CmG'CkG

Cuts at:      0      24     154     432     492    1761 
   Size:         24     130     278      60    1269

  Fragments arranged by size: 

                1269     278     130      60      24
 
NdeI CA'TA_TG

Cuts at:      0     408    1761 
   Size:        408    1353
 
NsiI A_TGCA'T

Cuts at:      0     590    1761 
   Size:        590    1171
 
NspI r_CATG'y

Cuts at:      0    1258    1761 
   Size:       1258     503
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     172     198    1131    1761 
   Size:        172      26     933     630

  Fragments arranged by size: 

                 933     630     172      26
 
PsiI TTA'TAA

Cuts at:      0     299     910    1411    1445    1761 
   Size:        299     611     501      34     316

  Fragments arranged by size: 

                 611     501     316     299      34
 
PspXI vC'TCGA_Gb

Cuts at:      0     767    1761 
   Size:        767     994
 
PvuII CAG'CTG

Cuts at:      0      24     154    1761 
   Size:         24     130    1607

  Fragments arranged by size: 

                1607     130      24
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1666    1761 
   Size:       1666      95
 
SmlI C'TyrA_G

Cuts at:      0     633     767     808     949    1761 
   Size:        633     134      41     141     812

  Fragments arranged by size: 

                 812     633     141     134      41
 
SpeI A'CTAG_T

Cuts at:      0     445    1761 
   Size:        445    1316
 
SspI AAT'ATT

Cuts at:      0     170    1761 
   Size:        170    1591
 
StyI C'CwwG_G

Cuts at:      0     980    1546    1761 
   Size:        980     566     215

  Fragments arranged by size: 

                 980     566     215
 
TatI w'GTAC_w

Cuts at:      0     759     812    1761 
   Size:        759      53     949

  Fragments arranged by size: 

                 949     759      53
 
XbaI T'CTAG_A

Cuts at:      0    1176    1761 
   Size:       1176     585
 
XhoI C'TCGA_G

Cuts at:      0     633     767    1761 
   Size:        633     134     994

  Fragments arranged by size: 

                 994     633     134
 
XmnI GAAnn'nnTTC

Cuts at:      0     684    1503    1761 
   Size:        684     819     258

  Fragments arranged by size: 

                 819     684     258
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AlfI     AloI    ApaLI     ApoI     AseI
     AvaI     BanI    BanII     BbsI     BcgI    BfrBI Bme1580I     BmrI
    BpuEI    BsaBI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BsrGI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     NdeI     NsiI     NspI    PflMI
     PsiI    PspXI    PvuII     SapI     SmlI     SpeI     SspI     StyI
     TatI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI    AlwNI     ApaI     AscI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XcmI     XmaI     ZraI
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