Restriction map for pF1KSDA0848
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AgeI        AhdI        AlwNI       Aor51HI     AscI        
Asp718I     AspEI       BamHI       BbeI        BbvCI       
BcgI        BciVI       BclI        BglI        BglII       
BlpI        Bpu1102I    BsaBI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       FauI        
FbaI        FseI        HaeII       HpaI        KasI        
KpnI        KspI        MamI        MluI        MroI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI         2168  GTCGAC               GT^[AC][GT]AC
AflII         370  CTTAAG               C^TTAAG
AflIII        709  ACATGT               A^C[GA][CT]GT
Alw44I       2743  GTGCAC               G^TGCAC
AocI         1599  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2743  GTGCAC               G^TGCAC
AseI          316  ATTAAT               AT^TAAT
AsnI          316  ATTAAT               AT^TAAT
AspI         1705  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2860  CTCGAG               C^[CT]CG[GA]G
AviII        1805  TGCGCA               TGC^GCA
AvrII        2373  CCTAGG               C^CTAGG
BalI          916  TGGCCA               TGG^CCA
BanI         1902  GGCACC               G^G[CT][GA]CC
BbrPI        2740  CACGTG               CAC^GTG
BfrI          370  CTTAAG               C^TTAAG
BlnI         2373  CCTAGG               C^CTAGG
BsaI          784  GAGACC               GGTCTC(1/5)
BsaWI        2423  ACCGGA               [AT]^CCGG[AT]
BsoBI        2860  CTCGAG               C^[CT]CG[GA]G
BspLU11I      709  ACATGT               A^CATGT
BssSI        2273  CTCGTG               CACGAG(-5/-1)
BstAPI       2314  GCAGGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2238  GGTTACC              G^GT[ACGT]ACC
BstPI        2238  GGTTACC              G^GT[ACGT]ACC
BstXI         795  CCACTTCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1302  GGATCT               [GA]^GATC[CT]
Bsu36I       1599  CCTGAGG              CC^T[ACGT]AGG
CfoI         1806  GCGC                 GCG^C
DraIII       1122  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          916  TGGCCA               [CT]^GGCC[GA]
Eco81I       1599  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2238  GGTTACC              G^GT[ACGT]ACC
FspI         1805  TGCGCA               TGC^GCA
HhaI         1806  GCGC                 GCG^C
HinP1I       1806  GCGC                 G^CGC
HindIII      1046  AAGCTT               A^AGCTT
MfeI           65  CAATTG               C^AATTG
MflI         1302  GGATCT               [GA]^GATC[CT]
MluNI         916  TGGCCA               TGG^CCA
MscI          916  TGGCCA               TGG^CCA
MunI           65  CAATTG               C^AATTG
NcoI          801  CCATGG               C^CATGG
PaeR7I       2860  CTCGAG               C^TCGAG
PflFI        1705  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2521  CCAGCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2740  CACGTG               CAC^GTG
PmlI         2740  CACGTG               CAC^GTG
PpuMI         809  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         316  ATTAAT               AT^TAAT
SacI          676  GAGCTC               GAGCT^C
SalI         2168  GTCGAC               G^TCGAC
SexAI        1706  ACCTGGT              A^CC[AT]GGT
SphI         2756  GCATGC               GCATG^C
SspI          202  AATATT               AAT^ATT
Tth111I      1705  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2521  CCAGCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          450  TCTAGA               T^CTAGA
XhoI         2860  CTCGAG               C^TCGAG
XhoII        1302  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcyI         1564  GACGCC               G[GA]^CG[CT]C
             2042  GGCGTC              
ApaI         1944  GGGCCC               GGGCC^C
             2453  GGGCCC              
BsaHI        1564  GACGCC               G[GA]^CG[CT]C
             2042  GGCGTC              
Bsp1407I      706  TGTACA               T^GTACA
              720  TGTACA              
BsrGI         706  TGTACA               T^GTACA
              720  TGTACA              
EcoNI          96  CCTAATAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2620  CCTGGGGGAGG         
EcoRI         258  GAATTC               G^AATTC
              881  GAATTC              
EcoT22I       332  ATGCAT               ATGCA^T
              911  ATGCAT              
HgaI         1564  GACGC                GACGC(5/10)
             2043  GCGTC               
Hin1I        1564  GACGCC               G[GA]^CG[CT]C
             2042  GGCGTC              
HincII       1168  GTCAAC               GT[CT]^[GA]AC
             2168  GTCGAC              
HindII       1168  GTCAAC               GT[CT]^[GA]AC
             2168  GTCGAC              
MspA1I        682  CAGCTG               C[AC]G^C[GT]G
             1856  CAGCTG              
NsiI          332  ATGCAT               ATGCA^T
              911  ATGCAT              
PvuII         682  CAGCTG               CAG^CTG
             1856  CAGCTG              
SmlI          370  CTTAAG               C^T[CT][GA]AG
             2860  CTCGAG              
SspBI         706  TGTACA               T^GTACA
              720  TGTACA              
StuI         1431  AGGCCT               AGG^CCT
             2016  AGGCCT              
XcmI         2517  CCACCCAGCAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2586  CCATGAGAAAAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI        
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