Restriction map for pF1KSDA0848
 (Linear) (Six-base) MAPSORT of: KIAA0848.seq  Check: 9361  from: 1  to: 2931



KIAA0848.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AccI GT'mk_AC

Cuts at:      0    2169    2931 
   Size:       2169     762
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     402     748    1482    2046    2931 
   Size:        402     346     734     564     885

  Fragments arranged by size: 

                 885     734     564     402     346
 
AflII C'TTAA_G

Cuts at:      0     370    2931 
   Size:        370    2561
 
AflIII A'CryG_T

Cuts at:      0     709    2931 
   Size:        709    2222
 
AleI CACnn'nnGTG

Cuts at:      0    2488    2931 
   Size:       2488     443
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2122    2931 
   Size:       2122     809
 
ApaI G_GGCC'C

Cuts at:      0    1948    2457    2931 
   Size:       1948     509     474

  Fragments arranged by size: 

                1948     509     474
 
ApaLI G'TGCA_C

Cuts at:      0    2743    2931 
   Size:       2743     188
 
ApoI r'AATT_y

Cuts at:      0     258     881    1287    2931 
   Size:        258     623     406    1644

  Fragments arranged by size: 

                1644     623     406     258
 
AseI AT'TA_AT

Cuts at:      0     317    2931 
   Size:        317    2614
 
AvaI C'yCGr_G

Cuts at:      0    2860    2931 
   Size:       2860      71
 
AvrII C'CTAG_G

Cuts at:      0    2373    2931 
   Size:       2373     558
 
BanI G'GyrC_C

Cuts at:      0    1902    2931 
   Size:       1902    1029
 
BanII G_rGCy'C

Cuts at:      0     128     680    1780    1948    2457    2931 
   Size:        128     552    1100     168     509     474

  Fragments arranged by size: 

                1100     552     509     474     168     128
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     408     835    2920    2931 
   Size:        408     427    2085      11

  Fragments arranged by size: 

                2085     427     408      11
 
BfrBI ATG'CAT

Cuts at:      0     334     913    2931 
   Size:        334     579    2018

  Fragments arranged by size: 

                2018     579     334
 
Bme1580I G_kGCm'C

Cuts at:      0    1905    1948    2457    2723    2747    2931 
   Size:       1905      43     509     266      24     184

  Fragments arranged by size: 

                1905     509     266     184      43      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1133    2567    2931 
   Size:       1133    1434     364

  Fragments arranged by size: 

                1434    1133     364
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     664    1880    1901    2171    2929    2931 
   Size:        374     290    1216      21     270     758       2

  Fragments arranged by size: 

                1216     758     374     290     270      21       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     998    2931 
   Size:        998    1933
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     778    2931 
   Size:        778    2153
 
BsaAI yAC'GTr

Cuts at:      0     610    2031    2742    2931 
   Size:        610    1421     711     189

  Fragments arranged by size: 

                1421     711     610     189
 
BsaHI Gr'CG_yC

Cuts at:      0    1565    2043    2931 
   Size:       1565     478     888

  Fragments arranged by size: 

                1565     888     478
 
BsaWI w'CCGG_w

Cuts at:      0    2423    2931 
   Size:       2423     508
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1925    2293    2296    2299    2931 
   Size:       1925     368       3       3     632

  Fragments arranged by size: 

                1925     632     368       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1869    1874    2474    2520    2931 
   Size:       1869       5     600      46     411

  Fragments arranged by size: 

                1869     600     411      46       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1680    1828    2342    2667    2722    2931 
   Size:       1680     148     514     325      55     209

  Fragments arranged by size: 

                1680     514     325     209     148      55
 
BsiHKAI G_wGCw'C

Cuts at:      0     680    2036    2747    2931 
   Size:        680    1356     711     184

  Fragments arranged by size: 

                1356     711     680     184
 
BsmI GAATG_Cn'

Cuts at:      0     650     746     915    1230    1659    2931 
   Size:        650      96     169     315     429    1272

  Fragments arranged by size: 

                1272     650     429     315     169      96
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     680    1780    1905    1948    2036    2457    2723 
   Size:        128     552    1100     125      43      88     421     266

Cuts at:   2723    2747    2931 
   Size:         24     184

  Fragments arranged by size: 

                1100     552     421     266     184     128     125      88
                  43      24
 
BsrDI GCAATG_nn'

Cuts at:      0     334    1076    2116    2931 
   Size:        334     742    1040     815

  Fragments arranged by size: 

                1040     815     742     334
 
BsrGI T'GTAC_A

Cuts at:      0     706     720    2931 
   Size:        706      14    2211

  Fragments arranged by size: 

                2211     706      14
 
BssSI C'ACGA_G

Cuts at:      0    2273    2931 
   Size:       2273     658
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2320    2931 
   Size:       2320     611
 
BstEII G'GTnAC_C

Cuts at:      0    2238    2931 
   Size:       2238     693
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     802    2931 
   Size:        802    2129
 
BstYI r'GATC_y

Cuts at:      0    1302    2931 
   Size:       1302    1629
 
Bsu36I CC'TnA_GG

Cuts at:      0    1600    2931 
   Size:       1600    1331
 
BtgI C'CryG_G

Cuts at:      0     801    1628    2204    2931 
   Size:        801     827     576     727

  Fragments arranged by size: 

                 827     801     727     576
 
BtsI GCAGTG_nn'

Cuts at:      0    2468    2931 
   Size:       2468     463
 
DraI TTT'AAA

Cuts at:      0     240     380     539    1719    2931 
   Size:        240     140     159    1180    1212

  Fragments arranged by size: 

                1212    1180     240     159     140
 
DraIII CAC_nnn'GTG

Cuts at:      0    1127    2931 
   Size:       1127    1804
 
EaeI y'GGCC_r

Cuts at:      0     916    2931 
   Size:        916    2015
 
EarI CTCTTCn'nnn_

Cuts at:      0     724    1387    2812    2931 
   Size:        724     663    1425     119

  Fragments arranged by size: 

                1425     724     663     119
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2165    2569    2931 
   Size:       2165     404     362

  Fragments arranged by size: 

                2165     404     362
 
EcoICRI GAG'CTC

Cuts at:      0     678    2931 
   Size:        678    2253
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     402     664     748    1482    1880    1901    2046 
   Size:        374      28     262      84     734     398      21     145

Cuts at:   2046    2171    2929    2931 
   Size:        125     758       2

  Fragments arranged by size: 

                 758     734     398     374     262     145     125      84
                  28      21       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     100    2624    2931 
   Size:        100    2524     307

  Fragments arranged by size: 

                2524     307     100
 
EcoO109I rG'GnC_Cy

Cuts at:      0     810    1217    1349    1944    1945    2196    2454    2931 
   Size:        810     407     132     595       1     251     258     477

  Fragments arranged by size: 

                 810     595     477     407     258     251     132       1
            
 
EcoRI G'AATT_C

Cuts at:      0     258     881    2931 
   Size:        258     623    2050

  Fragments arranged by size: 

                2050     623     258
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1398    1629    2076    2818    2931 
   Size:       1398     231     447     742     113

  Fragments arranged by size: 

                1398     742     447     231     113
 
FspI TGC'GCA

Cuts at:      0    1807    2931 
   Size:       1807    1124
 
FspAI rTGC'GCAy

Cuts at:      0    1807    2931 
   Size:       1807    1124
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     211     243     375     407    1088    1120    1236    1268 
   Size:        211      32     132      32     681      32     116      32

Cuts at:   1268    2484    2516    2931 
   Size:       1216      32     415

  Fragments arranged by size: 

                1216     681     415     211     132     116      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1170    2170    2931 
   Size:       1170    1000     761

  Fragments arranged by size: 

                1170    1000     761
 
HindIII A'AGCT_T

Cuts at:      0    1046    2931 
   Size:       1046    1885
 
MfeI C'AATT_G

Cuts at:      0      65    2931 
   Size:         65    2866
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     589    2931 
   Size:        589    2342
 
MscI TGG'CCA

Cuts at:      0     918    2931 
   Size:        918    2013
 
MslI CAynn'nnrTG

Cuts at:      0     556     800    1148    1652    1799    2104    2216    2488 
   Size:        556     244     348     504     147     305     112     272

Cuts at:   2488    2931 
   Size:        443

  Fragments arranged by size: 

                 556     504     443     348     305     272     244     147
                 112
 
MspA1I CmG'CkG

Cuts at:      0     684    1858    2931 
   Size:        684    1174    1073

  Fragments arranged by size: 

                1174    1073     684
 
NcoI C'CATG_G

Cuts at:      0     801    2931 
   Size:        801    2130
 
NsiI A_TGCA'T

Cuts at:      0     336     915    2931 
   Size:        336     579    2016

  Fragments arranged by size: 

                2016     579     336
 
NspI r_CATG'y

Cuts at:      0     713    1425    2760    2931 
   Size:        713     712    1335     171

  Fragments arranged by size: 

                1335     713     712     171
 
PciI A'CATG_T

Cuts at:      0     709    2931 
   Size:        709    2222
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2527    2931 
   Size:       2527     404
 
PfoI T'CCnGG_A

Cuts at:      0    1475    1640    2906    2931 
   Size:       1475     165    1266      25

  Fragments arranged by size: 

                1475    1266     165      25
 
PmlI CAC'GTG

Cuts at:      0    2742    2931 
   Size:       2742     189
 
PpuMI rG'GwC_Cy

Cuts at:      0     810    2931 
   Size:        810    2121
 
PspOMI G'GGCC_C

Cuts at:      0    1944    2453    2931 
   Size:       1944     509     478

  Fragments arranged by size: 

                1944     509     478
 
PspXI vC'TCGA_Gb

Cuts at:      0    2860    2931 
   Size:       2860      71
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1447    1479    2931 
   Size:       1447      32    1452

  Fragments arranged by size: 

                1452    1447      32
 
PstI C_TGCA'G

Cuts at:      0     254     464    1494    2016    2931 
   Size:        254     210    1030     522     915

  Fragments arranged by size: 

                1030     915     522     254     210
 
PvuII CAG'CTG

Cuts at:      0     684    1858    2931 
   Size:        684    1174    1073

  Fragments arranged by size: 

                1174    1073     684
 
SacI G_AGCT'C

Cuts at:      0     680    2931 
   Size:        680    2251
 
SalI G'TCGA_C

Cuts at:      0    2168    2931 
   Size:       2168     763
 
SexAI A'CCwGG_T

Cuts at:      0    1706    2931 
   Size:       1706    1225
 
SfcI C'TryA_G

Cuts at:      0     250     460    1435    1490    2012    2931 
   Size:        250     210     975      55     522     919

  Fragments arranged by size: 

                 975     919     522     250     210      55
 
SmlI C'TyrA_G

Cuts at:      0     370    2860    2931 
   Size:        370    2490      71

  Fragments arranged by size: 

                2490     370      71
 
SphI G_CATG'C

Cuts at:      0    2760    2931 
   Size:       2760     171
 
SspI AAT'ATT

Cuts at:      0     204    2931 
   Size:        204    2727
 
StuI AGG'CCT

Cuts at:      0    1433    2018    2931 
   Size:       1433     585     913

  Fragments arranged by size: 

                1433     913     585
 
StyI C'CwwG_G

Cuts at:      0     435     697     801    1024    1155    1213    1298    1687 
   Size:        435     262     104     223     131      58      85     389

Cuts at:   1687    2373    2402    2931 
   Size:        686      29     529

  Fragments arranged by size: 

                 686     529     435     389     262     223     131     104
                  85      58      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1115    2350    2415    2534    2931 
   Size:       1115    1235      65     119     397

  Fragments arranged by size: 

                1235    1115     397     119      65
 
TatI w'GTAC_w

Cuts at:      0     706     720    1454    2222    2931 
   Size:        706      14     734     768     709

  Fragments arranged by size: 

                 768     734     709     706      14
 
Tth111I GACn'n_nGTC

Cuts at:      0    1708    2931 
   Size:       1708    1223
 
XbaI T'CTAG_A

Cuts at:      0     450    2931 
   Size:        450    2481
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2524    2593    2931 
   Size:       2524      69     338

  Fragments arranged by size: 

                2524     338      69
 
XhoI C'TCGA_G

Cuts at:      0    2860    2931 
   Size:       2860      71
 
XmnI GAAnn'nnTTC

Cuts at:      0     746    1208    1612    2801    2931 
   Size:        746     462     404    1189     130

  Fragments arranged by size: 

                1189     746     462     404     130
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AleI     AlfI     ApaI    ApaLI
     ApoI     AseI     AvaI    AvrII     BanI    BanII     BbsI    BfrBI
 Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
     DraI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    FspAI    Hin4I   HincII  HindIII
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
     PstI    PvuII     SacI     SalI    SexAI     SfcI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AloI
    AlwNI     AscI    AsiSI     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BpuEI    BsaBI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BstBI  BstZ17I    BtgZI     ClaI
    CspCI     DrdI     EagI    EcoRV     FseI    HaeII     HpaI     KasI
     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PmeI     PpiI    PshAI     PsiI     PvuI
    RsrII    SacII    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     TstI
     XmaI     ZraI
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