Restriction map for pF1KSDA0846
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BfrI        BglI        BlnI        BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspEI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      Cfr10I      CpoI        DraI        
DraIII      EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KspI        MamI        MluI        MluNI       
MroI        MscI        MspA1I      MvnI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SspBI       
SspI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1194  GGTACC               G^GTACC
AccI         1996  GTAGAC               GT^[AC][GT]AC
AflIII        339  ACACGT               A^C[GA][CT]GT
AhdI         1876  GACTCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I         48  GTGCAC               G^TGCAC
AlwNI        1859  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          48  GTGCAC               G^TGCAC
Asp700I       157  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1194  GGTACC               G^GTACC
AspEI        1876  GACTCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII         454  GAGCCC               G[GA]GC[CT]^C
BbeI         1644  GGCGCC               GGCGC^C
BcgI         1521  CGAACACTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          469  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      469  GCTGAGC              GC^T[ACGT]AGC
BspDI         579  ATCGAT               AT^CGAT
BspHI         230  TCATGA               T^CATGA
BspMI        1073  ACCTGC               ACCTGC(4/8)
BsrDI         200  GCAATG               GCAATG(2/0)
BstAPI        845  GCAATTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         469  GCTGAGC              GC^T[ACGT]AGC
ClaI          579  ATCGAT               AT^CGAT
DraII        1882  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI         1234  GACCCCACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1876  GACTCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1882  GGGTCCC              [GA]G^G[ACGT]CC[CT]
FauI         1531  GCGGG                CCCGC(4/6)
HaeII        1644  GGCGCC               [GA]GCGC^[CT]
HgaI         1176  GACGC                GACGC(5/10)
HincII        320  GTCAAC               GT[CT]^[GA]AC
HindII        320  GTCAAC               GT[CT]^[GA]AC
KasI         1644  GGCGCC               G^GCGCC
KpnI         1194  GGTACC               GGTAC^C
MaeII         341  ACGT                 A^CGT
MfeI         1587  CAATTG               C^AATTG
MunI         1587  CAATTG               C^AATTG
NarI         1644  GGCGCC               GG^CGCC
NcoI          543  CCATGG               C^CATGG
PflMI         615  CCAGTTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1882  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          230  TCATGA               T^CATGA
SapI         1784  GCTCTTC              GCTCTTC(1/4)
SbfI         1625  CCTGCAGG             CCTGCA^GG
SfoI         1644  GGCGCC               GGC^GCC
SmaI         1640  CCCGGG               CCC^GGG
SpeI         1017  ACTAGT               A^CTAGT
SphI         1977  GCATGC               GCATG^C
Sse8387I     1625  CCTGCAGG             CCTGCA^GG
Van91I        615  CCAGTTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1181  CCAACAAGCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1640  CCCGGG               C^CCGGG
XmaI         1640  CCCGGG               C^CCGGG
XmnI          157  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          861  CCGC                 CCGC(-3/-1)
             1531  GCGG                
AcyI         1176  GACGCC               G[GA]^CG[CT]C
             1644  GGCGCC              
AspHI          48  GTGCAC               G[AT]GC[AT]^C
              472  GAGCAC              
AvaI         1640  CCCGGG               C^[CT]CG[GA]G
             1878  CTCGGG              
BanI         1194  GGTACC               G^G[CT][GA]CC
             1644  GGCGCC              
BciVI        1561  GGATAC               GTATCC(6/5)
              412  GTATCC              
BclI         1067  TGATCA               T^GATCA
             1293  TGATCA              
BglII         218  AGATCT               A^GATCT
             1796  AGATCT              
BsaHI        1176  GACGCC               G[GA]^CG[CT]C
             1644  GGCGCC              
BseRI         834  CTCCTC               GAGGAG(10/8)
              961  GAGGAG              
BsiHKAI        48  GTGCAC               G[AT]GC[AT]^C
              472  GAGCAC              
BsmI         1032  GAATGC               GAATGC(1/-1)
              365  GCATTC              
BsoBI        1640  CCCGGG               C^[CT]CG[GA]G
             1878  CTCGGG              
CfoI         1645  GCGC                 GCG^C
             1702  GCGC                
DsaI          543  CCATGG               C^C[GA][CT]GG
             1238  CCACGG              
FbaI         1067  TGATCA               T^GATCA
             1293  TGATCA              
HapII        1641  CCGG                 C^CGG
             2001  CCGG                
HhaI         1645  GCGC                 GCG^C
             1702  GCGC                
Hin1I        1176  GACGCC               G[GA]^CG[CT]C
             1644  GGCGCC              
HinP1I       1645  GCGC                 G^CGC
             1702  GCGC                
HpaII        1641  CCGG                 C^CGG
             2001  CCGG                
MspI         1641  CCGG                 C^CGG
             2001  CCGG                
NspI          170  GCATGT               [GA]CATG^[CT]
             1977  GCATGC              
SfaNI         120  GATGC                GCATC(5/9)
             1221  GATGC               
SmlI         1504  CTCAAG               C^T[CT][GA]AG
             1653  CTTGAG              
StuI          738  AGGCCT               AGG^CCT
              866  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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