Restriction map for pF1KSDA0841
 (Linear) (Six-base) MAPSORT of: KIAA0841.seq  Check: 3575  from: 1  to: 1899



KIAA0841.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1008    1899 
   Size:       1008     891
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     955    1732    1899 
   Size:        955     777     167

  Fragments arranged by size: 

                 955     777     167
 
AfeI AGC'GCT

Cuts at:      0    1484    1899 
   Size:       1484     415
 
AflIII A'CryG_T

Cuts at:      0     993    1899 
   Size:        993     906
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     767    1402    1899 
   Size:        767     635     497

  Fragments arranged by size: 

                 767     635     497
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     447     594     681     858     972    1023    1092 
   Size:        291     156     147      87     177     114      51      69

Cuts at:   1092    1122    1653    1710    1743    1833    1890    1899 
   Size:         30     531      57      33      90      57       9

  Fragments arranged by size: 

                 531     291     177     156     147     114      90      87
                  69      57      57      51      33      30       9
 
ApaI G_GGCC'C

Cuts at:      0      78     393     588    1881    1899 
   Size:         78     315     195    1293      18

  Fragments arranged by size: 

                1293     315     195      78      18
 
ApaLI G'TGCA_C

Cuts at:      0     148    1899 
   Size:        148    1751
 
AvaI C'yCGr_G

Cuts at:      0      71    1251    1899 
   Size:         71    1180     648

  Fragments arranged by size: 

                1180     648      71
 
BamHI G'GATC_C

Cuts at:      0     511    1003    1154    1899 
   Size:        511     492     151     745

  Fragments arranged by size: 

                 745     511     492     151
 
BanI G'GyrC_C

Cuts at:      0      57     464    1166    1308    1899 
   Size:         57     407     702     142     591

  Fragments arranged by size: 

                 702     591     407     142      57
 
BanII G_rGCy'C

Cuts at:      0      78     378     393     545     588     688    1064    1252 
   Size:         78     300      15     152      43     100     376     188

Cuts at:   1252    1471    1823    1881    1899 
   Size:        219     352      58      18

  Fragments arranged by size: 

                 376     352     300     219     188     152     100      78
                  58      43      18      15
 
BbeI G_GCGC'C

Cuts at:      0     468    1170    1899 
   Size:        468     702     729

  Fragments arranged by size: 

                 729     702     468
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     417    1624    1899 
   Size:        417    1207     275

  Fragments arranged by size: 

                1207     417     275
 
BbvCI CC'TCA_GC

Cuts at:      0    1126    1899 
   Size:       1126     773
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     554     588    1899 
   Size:        554      34    1311

  Fragments arranged by size: 

                1311     554      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     968    1848    1876    1899 
   Size:        968     880      28      23

  Fragments arranged by size: 

                 968     880      28      23
 
BlpI GC'TnA_GC

Cuts at:      0     682    1486    1899 
   Size:        682     804     413

  Fragments arranged by size: 

                 804     682     413
 
Bme1580I G_kGCm'C

Cuts at:      0      62      78     152     393     588    1381    1881    1899 
   Size:         62      16      74     241     195     793     500      18

  Fragments arranged by size: 

                 793     500     241     195      74      62      18      16
            
 
BmgBI CAC'GTC

Cuts at:      0     346     521     726    1899 
   Size:        346     175     205    1173

  Fragments arranged by size: 

                1173     346     205     175
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      37     582    1039    1839    1899 
   Size:         37     545     457     800      60

  Fragments arranged by size: 

                 800     545     457      60      37
 
BmtI G_CTAG'C

Cuts at:      0      10    1267    1899 
   Size:         10    1257     632

  Fragments arranged by size: 

                1257     632      10
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     397    1899 
   Size:        397    1502
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     559     831     949    1360    1455    1493    1741 
   Size:        313     246     272     118     411      95      38     248

Cuts at:   1741    1765    1899 
   Size:         24     134

  Fragments arranged by size: 

                 411     313     272     248     246     134     118      95
                  38      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     320    1126    1757    1899 
   Size:        320     806     631     142

  Fragments arranged by size: 

                 806     631     320     142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341    1048    1899 
   Size:        341     707     851

  Fragments arranged by size: 

                 851     707     341
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     918    1177    1899 
   Size:        918     259     722

  Fragments arranged by size: 

                 918     722     259
 
BsaHI Gr'CG_yC

Cuts at:      0     465    1167    1899 
   Size:        465     702     732

  Fragments arranged by size: 

                 732     702     465
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     370     373     994    1195    1357    1831    1899 
   Size:        370       3     621     201     162     474      68

  Fragments arranged by size: 

                 621     474     370     201     162      68       3
 
BseYI C'CCAG_C

Cuts at:      0      35     365     397     917     956    1105    1497    1598 
   Size:         35     330      32     520      39     149     392     101

Cuts at:   1598    1859    1899 
   Size:        261      40

  Fragments arranged by size: 

                 520     392     330     261     149     101      40      39
                  35      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     558     804    1057    1144    1432    1899 
   Size:        558     246     253      87     288     467

  Fragments arranged by size: 

                 558     467     288     253     246      87
 
BsiEI CG_ry'CG

Cuts at:      0      44    1899 
   Size:         44    1855
 
BsiHKAI G_wGCw'C

Cuts at:      0     152     378     688     746     928    1064    1823    1899 
   Size:        152     226     310      58     182     136     759      76

  Fragments arranged by size: 

                 759     310     226     182     152     136      76      58
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     688     984    1899 
   Size:        688     296     915

  Fragments arranged by size: 

                 915     688     296
 
Bsp1286I G_dGCh'C

Cuts at:      0      62      78     152     378     393     545     588     688 
   Size:         62      16      74     226      15     152      43     100

Cuts at:    688     746     928    1064    1252    1381    1471    1823    1881 
   Size:         58     182     136     188     129      90     352      58

Cuts at:   1881    1899 
   Size:         18

  Fragments arranged by size: 

                 352     226     188     182     152     136     129     100
                  90      74      62      58      58      43      18      16
                  15
 
BspHI T'CATG_A

Cuts at:      0     645    1899 
   Size:        645    1254
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1008    1899 
   Size:       1008     891
 
BsrBI CCG'CTC

Cuts at:      0     759     942    1899 
   Size:        759     183     957

  Fragments arranged by size: 

                 957     759     183
 
BsrFI r'CCGG_y

Cuts at:      0    1536    1899 
   Size:       1536     363
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     147     447    1122    1899 
   Size:        147     300     675     777

  Fragments arranged by size: 

                 777     675     300     147
 
BstYI r'GATC_y

Cuts at:      0     173     432     511    1003    1154    1602    1661    1899 
   Size:        173     259      79     492     151     448      59     238

  Fragments arranged by size: 

                 492     448     259     238     173     151      79      59
            
 
BtgI C'CryG_G

Cuts at:      0      62     332     545     866    1421    1899 
   Size:         62     270     213     321     555     478

  Fragments arranged by size: 

                 555     478     321     270     213      62
 
BtsI GCAGTG_nn'

Cuts at:      0     682     792    1310    1899 
   Size:        682     110     518     589

  Fragments arranged by size: 

                 682     589     518     110
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     861    1899 
   Size:        861    1038
 
EaeI y'GGCC_r

Cuts at:      0     198     329    1223    1899 
   Size:        198     131     894     676

  Fragments arranged by size: 

                 894     676     198     131
 
EarI CTCTTCn'nnn_

Cuts at:      0      41    1556    1708    1899 
   Size:         41    1515     152     191

  Fragments arranged by size: 

                1515     191     152      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     909    1189    1899 
   Size:        909     280     710

  Fragments arranged by size: 

                 909     710     280
 
EcoICRI GAG'CTC

Cuts at:      0     376     686    1062    1821    1899 
   Size:        376     310     376     759      78

  Fragments arranged by size: 

                 759     376     376     310      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     559     831     949     955    1360    1455    1493 
   Size:        313     246     272     118       6     405      95      38

Cuts at:   1493    1732    1741    1765    1899 
   Size:        239       9      24     134

  Fragments arranged by size: 

                 405     313     272     246     239     134     118      95
                  38      24       9       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     934     976    1702    1813    1899 
   Size:        934      42     726     111      86

  Fragments arranged by size: 

                 934     726     111      86      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     828     969    1068    1560    1830    1877    1878 
   Size:         75     753     141      99     492     270      47       1

Cuts at:   1878    1899 
   Size:         21

  Fragments arranged by size: 

                 753     492     270     141      99      75      47      21
                   1
 
EcoRV GAT'ATC

Cuts at:      0     815    1899 
   Size:        815    1084
 
FspI TGC'GCA

Cuts at:      0      94    1899 
   Size:         94    1805
 
HaeII r_GCGC'y

Cuts at:      0     468     963    1044    1170    1486    1899 
   Size:        468     495      81     126     316     413

  Fragments arranged by size: 

                 495     468     413     316     126      81
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     760     792    1899 
   Size:        760      32    1107

  Fragments arranged by size: 

                1107     760      32
 
KasI G'GCGC_C

Cuts at:      0     464    1166    1899 
   Size:        464     702     733

  Fragments arranged by size: 

                 733     702     464
 
MluI A'CGCG_T

Cuts at:      0     993    1899 
   Size:        993     906
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     205    1899 
   Size:        205    1694
 
MscI TGG'CCA

Cuts at:      0     200    1899 
   Size:        200    1699
 
MslI CAynn'nnrTG

Cuts at:      0     331    1899 
   Size:        331    1568
 
MspA1I CmG'CkG

Cuts at:      0     444     921    1105    1152    1173    1446    1584    1899 
   Size:        444     477     184      47      21     273     138     315

  Fragments arranged by size: 

                 477     444     315     273     184     138      47      21
            
 
NaeI GCC'GGC

Cuts at:      0    1538    1899 
   Size:       1538     361
 
NarI GG'CG_CC

Cuts at:      0     465    1167    1899 
   Size:        465     702     732

  Fragments arranged by size: 

                 732     702     465
 
NcoI C'CATG_G

Cuts at:      0     332     866    1899 
   Size:        332     534    1033

  Fragments arranged by size: 

                1033     534     332
 
NgoMIV G'CCGG_C

Cuts at:      0    1536    1899 
   Size:       1536     363
 
NheI G'CTAG_C

Cuts at:      0       6    1263    1899 
   Size:          6    1257     636

  Fragments arranged by size: 

                1257     636       6
 
NspI r_CATG'y

Cuts at:      0     148    1899 
   Size:        148    1751
 
PasI CC'CwG_GG

Cuts at:      0    1872    1899 
   Size:       1872      27
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     291     729     972    1176    1497    1899 
   Size:        291     438     243     204     321     402

  Fragments arranged by size: 

                 438     402     321     291     243     204
 
PfoI T'CCnGG_A

Cuts at:      0     272     881    1899 
   Size:        272     609    1018

  Fragments arranged by size: 

                1018     609     272
 
PpuMI rG'GwC_Cy

Cuts at:      0    1560    1899 
   Size:       1560     339
 
PspOMI G'GGCC_C

Cuts at:      0      74     389     584    1877    1899 
   Size:         74     315     195    1293      22

  Fragments arranged by size: 

                1293     315     195      74      22
 
PstI C_TGCA'G

Cuts at:      0     452     473     599     756    1097    1577    1661    1766 
   Size:        452      21     126     157     341     480      84     105

Cuts at:   1766    1886    1899 
   Size:        120      13

  Fragments arranged by size: 

                 480     452     341     157     126     120     105      84
                  21      13
 
PvuII CAG'CTG

Cuts at:      0    1105    1173    1446    1899 
   Size:       1105      68     273     453

  Fragments arranged by size: 

                1105     453     273      68
 
SacI G_AGCT'C

Cuts at:      0     378     688    1064    1823    1899 
   Size:        378     310     376     759      76

  Fragments arranged by size: 

                 759     378     376     310      76
 
SbfI CC_TGCA'GG

Cuts at:      0     473    1577    1899 
   Size:        473    1104     322

  Fragments arranged by size: 

                1104     473     322
 
SfcI C'TryA_G

Cuts at:      0     448     469     595     752    1093    1135    1573    1657 
   Size:        448      21     126     157     341      42     438      84

Cuts at:   1657    1762    1882    1899 
   Size:        105     120      17

  Fragments arranged by size: 

                 448     438     341     157     126     120     105      84
                  42      21      17
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1876    1899 
   Size:       1876      23
 
SfoI GGC'GCC

Cuts at:      0     466    1168    1899 
   Size:        466     702     731

  Fragments arranged by size: 

                 731     702     466
 
SmaI CCC'GGG

Cuts at:      0      73    1253    1899 
   Size:         73    1180     646

  Fragments arranged by size: 

                1180     646      73
 
SmlI C'TyrA_G

Cuts at:      0     356    1063    1899 
   Size:        356     707     836

  Fragments arranged by size: 

                 836     707     356
 
SrfI GCCC'GGGC

Cuts at:      0      73    1899 
   Size:         73    1826
 
StuI AGG'CCT

Cuts at:      0    1519    1637    1899 
   Size:       1519     118     262

  Fragments arranged by size: 

                1519     262     118
 
StyI C'CwwG_G

Cuts at:      0     332     737     866    1462    1899 
   Size:        332     405     129     596     437

  Fragments arranged by size: 

                 596     437     405     332     129
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     379     764     892    1660    1899 
   Size:        379     385     128     768     239

  Fragments arranged by size: 

                 768     385     379     239     128
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     888     920    1441    1473    1899 
   Size:        888      32     521      32     426

  Fragments arranged by size: 

                 888     521     426      32      32
 
XmaI C'CCGG_G

Cuts at:      0      71    1251    1899 
   Size:         71    1180     648

  Fragments arranged by size: 

                1180     648      71
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AlfI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BstAPI
    BstYI     BtgI     BtsI     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FspI    HaeII    Hin4I     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI     SbfI     SfcI     SfiI     SfoI     SmaI
     SmlI     SrfI     StuI     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BciVI
     BclI    BfrBI    BglII    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI
     BsmI    BspEI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI    EcoRI     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     NdeI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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