Restriction map for pF1KSDA0817
 (Linear) (Six-base) MAPSORT of: KIAA0817.seq  Check: 5393  from: 1  to: 8334



KIAA0817.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      93     556     978    1122    1665    2419    2426    2728 
   Size:         93     463     422     144     543     754       7     302

Cuts at:   2728    4072    8334 
   Size:       1344    4262

  Fragments arranged by size: 

                4262    1344     754     543     463     422     302     144
                  93       7
 
AatII G_ACGT'C

Cuts at:      0    5580    8334 
   Size:       5580    2754
 
AccI GT'mk_AC

Cuts at:      0    4832    5574    8334 
   Size:       4832     742    2760

  Fragments arranged by size: 

                4832    2760     742
 
Acc65I G'GTAC_C

Cuts at:      0    2750    8334 
   Size:       2750    5584
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     912    1309    1327    1836    2638    5155    5468 
   Size:        351     561     397      18     509     802    2517     313

Cuts at:   5468    5892    8334 
   Size:        424    2442

  Fragments arranged by size: 

                2517    2442     802     561     509     424     397     351
                 313      18
 
AflIII A'CryG_T

Cuts at:      0     917    1545    3611    4859    6286    6961    8334 
   Size:        917     628    2066    1248    1427     675    1373

  Fragments arranged by size: 

                2066    1427    1373    1248     917     675     628
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3758    6824    6986    7164    8334 
   Size:       3758    3066     162     178    1170

  Fragments arranged by size: 

                3758    3066    1170     178     162
 
AleI CACnn'nnGTG

Cuts at:      0    2858    3610    4342    4774    5188    7255    7330    8334 
   Size:       2858     752     732     432     414    2067      75    1004

  Fragments arranged by size: 

                2858    2067    1004     752     732     432     414      75
            
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     370    5784    6079    6192    8334 
   Size:        370    5414     295     113    2142

  Fragments arranged by size: 

                5414    2142     370     295     113
 
AlwNI CAG_nnn'CTG

Cuts at:      0     108    1483    1709    1969    2242    2658    3308    3936 
   Size:        108    1375     226     260     273     416     650     628

Cuts at:   3936    4194    4401    4455    4743    4746    6075    6641    7704 
   Size:        258     207      54     288       3    1329     566    1063

Cuts at:   7704    7824    8334 
   Size:        120     510

  Fragments arranged by size: 

                1375    1329    1063     650     628     566     510     416
                 288     273     260     258     226     207     120     108
                  54       3
 
ApaI G_GGCC'C

Cuts at:      0      75     992    1333    2927    3325    4033    4231    4480 
   Size:         75     917     341    1594     398     708     198     249

Cuts at:   4480    5306    7427    7673    8027    8099    8171    8334 
   Size:        826    2121     246     354      72      72     163

  Fragments arranged by size: 

                2121    1594     917     826     708     398     354     341
                 249     246     198     163      75      72      72
 
ApaLI G'TGCA_C

Cuts at:      0     240    1399    1903    5808    6927    8334 
   Size:        240    1159     504    3905    1119    1407

  Fragments arranged by size: 

                3905    1407    1159    1119     504     240
 
ApoI r'AATT_y

Cuts at:      0    7246    8334 
   Size:       7246    1088
 
AvaI C'yCGr_G

Cuts at:      0      74     426     984    1715    3252    3278    3557    3748 
   Size:         74     352     558     731    1537      26     279     191

Cuts at:   3748    4033    4906    5006    7427    8107    8334 
   Size:        285     873     100    2421     680     227

  Fragments arranged by size: 

                2421    1537     873     731     680     558     352     285
                 279     227     191     100      74      26
 
AvrII C'CTAG_G

Cuts at:      0    1924    3213    8334 
   Size:       1924    1289    5121

  Fragments arranged by size: 

                5121    1924    1289
 
BamHI G'GATC_C

Cuts at:      0     206    3552    7440    8334 
   Size:        206    3346    3888     894

  Fragments arranged by size: 

                3888    3346     894     206
 
BanI G'GyrC_C

Cuts at:      0     117     544     697     963    1632    1653    1736    1885 
   Size:        117     427     153     266     669      21      83     149

Cuts at:   1885    2393    2478    2611    2702    2750    2804    2900    3050 
   Size:        508      85     133      91      48      54      96     150

Cuts at:   3050    3514    4534    4768    5480    6194    6353    7474    7675 
   Size:        464    1020     234     712     714     159    1121     201

Cuts at:   7675    7995    8185    8334 
   Size:        320     190     149

  Fragments arranged by size: 

                1121    1020     714     712     669     508     464     427
                 320     266     234     201     190     159     153     150
                 149     149     133     117      96      91      85      83
                  54      48      21
 
BanII G_rGCy'C

Cuts at:      0      75     959     992    1333    2324    2436    2927    3325 
   Size:         75     884      33     341     991     112     491     398

Cuts at:   3325    3488    3634    4009    4033    4094    4231    4480    4788 
   Size:        163     146     375      24      61     137     249     308

Cuts at:   4788    4913    5144    5306    5536    6082    6503    7193    7427 
   Size:        125     231     162     230     546     421     690     234

Cuts at:   7427    7673    7958    7988    8027    8099    8114    8171    8334 
   Size:        246     285      30      39      72      15      57     163

  Fragments arranged by size: 

                 991     884     690     546     491     421     398     375
                 341     308     285     249     246     234     231     230
                 163     163     162     146     137     125     112      75
                  72      61      57      39      33      30      24      15
            
 
BbeI G_GCGC'C

Cuts at:      0     121     701    6198    7478    7679    8189    8334 
   Size:        121     580    5497    1280     201     510     145

  Fragments arranged by size: 

                5497    1280     580     510     201     145     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1989    5745    6842    7740    7752    8334 
   Size:       1989    3756    1097     898      12     582

  Fragments arranged by size: 

                3756    1989    1097     898     582      12
 
BbvCI CC'TCA_GC

Cuts at:      0    3085    5166    8334 
   Size:       3085    2081    3168

  Fragments arranged by size: 

                3168    3085    2081
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    7594    7628    8334 
   Size:       7594      34     706

  Fragments arranged by size: 

                7594     706      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     431    1846    2597    2743    2998    3110    3386    4398 
   Size:        431    1415     751     146     255     112     276    1012

Cuts at:   4398    6310    6524    6902    7819    7953    8166    8334 
   Size:       1912     214     378     917     134     213     168

  Fragments arranged by size: 

                1912    1415    1012     917     751     431     378     276
                 255     214     213     168     146     134     112
 
BglII A'GATC_T

Cuts at:      0    1958    2300    8334 
   Size:       1958     342    6034

  Fragments arranged by size: 

                6034    1958     342
 
BlpI GC'TnA_GC

Cuts at:      0     522    1484    3811    7686    8334 
   Size:        522     962    2327    3875     648

  Fragments arranged by size: 

                3875    2327     962     648     522
 
Bme1580I G_kGCm'C

Cuts at:      0      75     244     427     992    1206    1333    1403    1637 
   Size:         75     169     183     565     214     127      70     234

Cuts at:   1637    1907    2249    2616    2927    3325    3446    3519    4033 
   Size:        270     342     367     311     398     121      73     514

Cuts at:   4033    4067    4231    4480    4523    5306    5812    6356    6931 
   Size:         34     164     249      43     783     506     544     575

Cuts at:   6931    7018    7427    7673    8027    8099    8171    8334 
   Size:         87     409     246     354      72      72     163

  Fragments arranged by size: 

                 783     575     565     544     514     506     409     398
                 367     354     342     311     270     249     246     234
                 214     183     169     164     163     127     121      87
                  75      73      72      72      70      43      34
 
BmgBI CAC'GTC

Cuts at:      0     598     918    2006    3021    5903    7166    7281    8334 
   Size:        598     320    1088    1015    2882    1263     115    1053

  Fragments arranged by size: 

                2882    1263    1088    1053    1015     598     320     115
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     946     960    1523    2902    3637    3969    4178    6822 
   Size:        946      14     563    1379     735     332     209    2644

Cuts at:   6822    7031    8334 
   Size:        209    1303

  Fragments arranged by size: 

                2644    1379    1303     946     735     563     332     209
                 209      14
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1977    4932    5139    6165    8334 
   Size:       1977    2955     207    1026    2169

  Fragments arranged by size: 

                2955    2169    1977    1026     207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     960    1070    2032    2338    3644    4586    4768    4962 
   Size:        960     110     962     306    1306     942     182     194

Cuts at:   4962    5134    5651    6726    8137    8334 
   Size:        172     517    1075    1411     197

  Fragments arranged by size: 

                1411    1306    1075     962     960     942     517     306
                 197     194     182     172     110
 
Bpu10I CC'TnA_GC

Cuts at:      0    1931    3085    5166    6164    8334 
   Size:       1931    1154    2081     998    2170

  Fragments arranged by size: 

                2170    2081    1931    1154     998
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    7603    8334 
   Size:       7603     731
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4267    4816    5854    7652    8334 
   Size:       4267     549    1038    1798     682

  Fragments arranged by size: 

                4267    1798    1038     682     549
 
BsaAI yAC'GTr

Cuts at:      0     245    1281    3149    4860    7530    8334 
   Size:        245    1036    1868    1711    2670     804

  Fragments arranged by size: 

                2670    1868    1711    1036     804     245
 
BsaHI Gr'CG_yC

Cuts at:      0     118     698    4571    5577    5644    6195    6294    7358 
   Size:        118     580    3873    1006      67     551      99    1064

Cuts at:   7358    7475    7676    8186    8334 
   Size:        117     201     510     148

  Fragments arranged by size: 

                3873    1064    1006     580     551     510     201     148
                 118     117      99      67
 
BsaWI w'CCGG_w

Cuts at:      0     203    5582    8334 
   Size:        203    5379    2752

  Fragments arranged by size: 

                5379    2752     203
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     259     289     306     336     769     799    1060    1090 
   Size:        259      30      17      30     433      30     261      30

Cuts at:   1090    1216    1246    1408    1438    1669    1699    1854    1884 
   Size:        126      30     162      30     231      30     155      30

Cuts at:   1884    2788    2818    5809    5839    5986    6016    7132    7162 
   Size:        904      30    2991      30     147      30    1116      30

Cuts at:   7162    8135    8165    8334 
   Size:        973      30     169

  Fragments arranged by size: 

                2991    1116     973     904     433     261     259     231
                 169     162     155     147     126      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     972    1068    2113    2518    2644    3592    3742    3966 
   Size:        972      96    1045     405     126     948     150     224

Cuts at:   3966    5329    6814    7463    7792    8334 
   Size:       1363    1485     649     329     542

  Fragments arranged by size: 

                1485    1363    1045     972     948     649     542     405
                 329     224     150     126      96
 
BseYI C'CCAG_C

Cuts at:      0      51     197     390     488     634    1585    2084    2111 
   Size:         51     146     193      98     146     951     499      27

Cuts at:   2111    2774    2996    4112    4404    4548    5520    7104    7386 
   Size:        663     222    1116     292     144     972    1584     282

Cuts at:   7386    7435    7479    7647    7951    8080    8092    8257    8334 
   Size:         49      44     168     304     129      12     165      77

  Fragments arranged by size: 

                1584    1116     972     951     663     499     304     292
                 282     222     193     168     165     146     146     144
                 129      98      77      51      49      44      27      12
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     595    1742    2032    2325    2511    3198    3789 
   Size:        343     252    1147     290     293     186     687     591

Cuts at:   3789    3940    4306    4906    5457    5697    5902    6429    7258 
   Size:        151     366     600     551     240     205     527     829

Cuts at:   7258    8212    8334 
   Size:        954     122

  Fragments arranged by size: 

                1147     954     829     687     600     591     551     527
                 366     343     293     290     252     240     205     186
                 151     122
 
BsiEI CG_ry'CG

Cuts at:      0    1602    7218    8334 
   Size:       1602    5616    1116

  Fragments arranged by size: 

                5616    1602    1116
 
BsiHKAI G_wGCw'C

Cuts at:      0     244     316     523     959    1403    1907    2563    3368 
   Size:        244      72     207     436     444     504     656     805

Cuts at:   3368    3747    4913    5812    6165    6931    7610    7802    8334 
   Size:        379    1166     899     353     766     679     192     532

  Fragments arranged by size: 

                1166     899     805     766     679     656     532     504
                 444     436     379     353     244     207     192      72
            
 
BsmI GAATG_Cn'

Cuts at:      0    1732    4063    6559    6583    8334 
   Size:       1732    2331    2496      24    1751

  Fragments arranged by size: 

                2496    2331    1751    1732      24
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2128    5584    6364    7003    8334 
   Size:       2128    3456     780     639    1331

  Fragments arranged by size: 

                3456    2128    1331     780     639
 
Bsp1286I G_dGCh'C

Cuts at:      0      75     244     316     427     523     959     992    1206 
   Size:         75     169      72     111      96     436      33     214

Cuts at:   1206    1333    1403    1637    1907    2249    2324    2436    2563 
   Size:        127      70     234     270     342      75     112     127

Cuts at:   2563    2616    2927    3325    3368    3446    3488    3519    3634 
   Size:         53     311     398      43      78      42      31     115

Cuts at:   3634    3747    4009    4033    4067    4094    4231    4480    4523 
   Size:        113     262      24      34      27     137     249      43

Cuts at:   4523    4788    4913    5144    5306    5536    5812    6082    6165 
   Size:        265     125     231     162     230     276     270      83

Cuts at:   6165    6356    6503    6931    7018    7193    7427    7610    7673 
   Size:        191     147     428      87     175     234     183      63

Cuts at:   7673    7802    7958    7988    8027    8099    8114    8171    8334 
   Size:        129     156      30      39      72      15      57     163

  Fragments arranged by size: 

                 436     428     398     342     311     276     270     270
                 265     262     249     234     234     231     230     214
                 191     183     175     169     163     162     156     147
                 137     129     127     127     125     115     113     112
                 111      96      87      83      78      75      75      72
                  72      70      63      57      53      43      43      42
                  39      34      33      31      30      27      24      15
            
 
BspEI T'CCGG_A

Cuts at:      0    5582    8334 
   Size:       5582    2752
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      93     395     556     617     978     994    1122    1665 
   Size:         93     302     161      61     361      16     128     543

Cuts at:   1665    2419    2426    2728    2839    3076    3208    3239    4072 
   Size:        754       7     302     111     237     132      31     833

Cuts at:   4072    5596    6098    6700    7646    8334 
   Size:       1524     502     602     946     688

  Fragments arranged by size: 

                1524     946     833     754     688     602     543     502
                 361     302     302     237     161     132     128     111
                  93      61      31      16       7
 
BsrBI CCG'CTC

Cuts at:      0    2330    2643    4437    4496    5288    7979    8334 
   Size:       2330     313    1794      59     792    2691     355

  Fragments arranged by size: 

                2691    2330    1794     792     355     313      59
 
BsrDI GCAATG_nn'

Cuts at:      0    1401    2436    2880    4164    4618    5265    5709    6201 
   Size:       1401    1035     444    1284     454     647     444     492

Cuts at:   6201    8334 
   Size:       2133

  Fragments arranged by size: 

                2133    1401    1284    1035     647     492     454     444
                 444
 
BsrFI r'CCGG_y

Cuts at:      0     862    1226    1601    2591    3380    3422    3562    4765 
   Size:        862     364     375     990     789      42     140    1203

Cuts at:   4765    5940    6421    6695    7580    7847    7947    8017    8334 
   Size:       1175     481     274     885     267     100      70     317

  Fragments arranged by size: 

                1203    1175     990     885     862     789     481     375
                 364     317     274     267     140     100      70      42
            
 
BsrGI T'GTAC_A

Cuts at:      0    1691    2787    4415    7368    8334 
   Size:       1691    1096    1628    2953     966

  Fragments arranged by size: 

                2953    1691    1628    1096     966
 
BssSI C'ACGA_G

Cuts at:      0    2854    3949    4837    7603    8230    8334 
   Size:       2854    1095     888    2766     627     104

  Fragments arranged by size: 

                2854    2766    1095     888     627     104
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     108     970    1736    3803    5480    5597    6935    7704 
   Size:        108     862     766    2067    1677     117    1338     769

Cuts at:   7704    8226    8334 
   Size:        522     108

  Fragments arranged by size: 

                2067    1677    1338     862     769     766     522     117
                 108     108
 
BstEII G'GTnAC_C

Cuts at:      0     763    7896    8334 
   Size:        763    7133     438

  Fragments arranged by size: 

                7133     763     438
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      30     634    1365    1806    2227    2386    2860    3124 
   Size:         30     604     731     441     421     159     474     264

Cuts at:   3124    4248    5026    5943    6946    8161    8334 
   Size:       1124     778     917    1003    1215     173

  Fragments arranged by size: 

                1215    1124    1003     917     778     731     604     474
                 441     421     264     173     159      30
 
BstYI r'GATC_y

Cuts at:      0     206    1958    2210    2300    3552    7440    8334 
   Size:        206    1752     252      90    1252    3888     894

  Fragments arranged by size: 

                3888    1752    1252     894     252     206      90
 
Bsu36I CC'TnA_GG

Cuts at:      0     342    1508    4106    6332    8334 
   Size:        342    1166    2598    2226    2002

  Fragments arranged by size: 

                2598    2226    2002    1166     342
 
BtgI C'CryG_G

Cuts at:      0      23     444     540     851    1178    1215    1608    1873 
   Size:         23     421      96     311     327      37     393     265

Cuts at:   1873    2800    2978    3058    3168    3459    4340    4530    4640 
   Size:        927     178      80     110     291     881     190     110

Cuts at:   4640    5186    5484    6396    6431    6548    7295    7489    7510 
   Size:        546     298     912      35     117     747     194      21

Cuts at:   7510    7566    8036    8334 
   Size:         56     470     298

  Fragments arranged by size: 

                 927     912     881     747     546     470     421     393
                 327     311     298     298     291     265     194     190
                 178     117     110     110      96      80      56      37
                  35      23      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2831    4472    6114    8334 
   Size:       2831    1641    1642    2220

  Fragments arranged by size: 

                2831    2220    1642    1641
 
BtsI GCAGTG_nn'

Cuts at:      0     457    1235    3099    3135    3186    3306    3366    3465 
   Size:        457     778    1864      36      51     120      60      99

Cuts at:   3465    3519    4125    4304    5598    5812    5959    6076    6507 
   Size:         54     606     179    1294     214     147     117     431

Cuts at:   6507    6603    6655    7123    7470    7559    8075    8143    8212 
   Size:         96      52     468     347      89     516      68      69

Cuts at:   8212    8271    8334 
   Size:         59      63

  Fragments arranged by size: 

                1864    1294     778     606     516     468     457     431
                 347     214     179     147     120     117      99      96
                  89      69      68      63      60      59      54      52
                  51      36
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3462    3497    6694    6729    8334 
   Size:       3462      35    3197      35    1605

  Fragments arranged by size: 

                3462    3197    1605      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     239     466     651    3460    4367    5480    7795    8334 
   Size:        239     227     185    2809     907    1113    2315     539

  Fragments arranged by size: 

                2809    2315    1113     907     539     239     227     185
            
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1705    7306    7357    7545    7585    7744    8334 
   Size:       1705    5601      51     188      40     159     590

  Fragments arranged by size: 

                5601    1705     590     188     159      51      40
 
EaeI y'GGCC_r

Cuts at:      0      20      44     848    1170    1599    2678    3165    3462 
   Size:         20      24     804     322     429    1079     487     297

Cuts at:   3462    3719    3900    4527    6107    6240    6948    7110    7215 
   Size:        257     181     627    1580     133     708     162     105

Cuts at:   7215    7507    7874    8138    8334 
   Size:        292     367     264     196

  Fragments arranged by size: 

                1580    1079     804     708     627     487     429     367
                 322     297     292     264     257     196     181     162
                 133     105      24      20
 
EagI C'GGCC_G

Cuts at:      0    1599    7215    8334 
   Size:       1599    5616    1119

  Fragments arranged by size: 

                5616    1599    1119
 
EarI CTCTTCn'nnn_

Cuts at:      0     370    1078    1093    2176    2614    7714    7786    8334 
   Size:        370     708      15    1083     438    5100      72     548

  Fragments arranged by size: 

                5100    1083     708     548     438     370      72      15
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     314     777    1087    1163    2444    3248    7255    7926 
   Size:        314     463     310      76    1281     804    4007     671

Cuts at:   7926    7963    8334 
   Size:         37     371

  Fragments arranged by size: 

                4007    1281     804     671     463     371     314     310
                  76      37
 
EcoICRI GAG'CTC

Cuts at:      0     957    4911    8334 
   Size:        957    3954    3423

  Fragments arranged by size: 

                3954    3423     957
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     912     960    1070    1309    1327    1836    2032 
   Size:        351     561      48     110     239      18     509     196

Cuts at:   2032    2338    2638    3644    4586    4768    4962    5134    5155 
   Size:        306     300    1006     942     182     194     172      21

Cuts at:   5155    5468    5651    5892    6726    8137    8334 
   Size:        313     183     241     834    1411     197

  Fragments arranged by size: 

                1411    1006     942     834     561     509     351     313
                 306     300     241     239     197     196     194     183
                 182     172     110      48      21      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      10    2422    4393    7807    8334 
   Size:         10    2412    1971    3414     527

  Fragments arranged by size: 

                3414    2412    1971     527      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71     186     496     663     744     810     988     989 
   Size:         71     115     310     167      81      66     178       1

Cuts at:    989    1329    1719    1772    1879    1984    2239    2462    2535 
   Size:        340     390      53     107     105     255     223      73

Cuts at:   2535    2923    2924    2964    3321    3322    3450    3752    3917 
   Size:        388       1      40     357       1     128     302     165

Cuts at:   3917    3933    4029    4030    4227    4228    4380    4476    4590 
   Size:         16      96       1     197       1     152      96     114

Cuts at:   4590    4629    5302    5303    5468    5489    5708    6136    6302 
   Size:         39     673       1     165      21     219     428     166

Cuts at:   6302    6689    6826    7210    7424    7515    7701    8024    8032 
   Size:        387     137     384     214      91     186     323       8

Cuts at:   8032    8168    8334 
   Size:        136     166

  Fragments arranged by size: 

                 673     428     390     388     387     384     357     340
                 323     310     302     255     223     219     214     197
                 186     178     167     166     166     165     165     152
                 137     136     128     115     114     107     105      96
                  96      91      81      73      71      66      53      40
                  39      21      16       8       1       1       1       1
                   1       1
 
FseI GG_CCGG'CC

Cuts at:      0    1605    8334 
   Size:       1605    6729
 
HaeII r_GCGC'y

Cuts at:      0     121     701    1525    2112    2154    5208    6198    7207 
   Size:        121     580     824     587      42    3054     990    1009

Cuts at:   7207    7478    7679    7848    8189    8334 
   Size:        271     201     169     341     145

  Fragments arranged by size: 

                3054    1009     990     824     587     580     341     271
                 201     169     145     121      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291    1060    1092    1669    1701    3115    3147 
   Size:        259      32     769      32     577      32    1414      32

Cuts at:   3147    3744    3776    6810    6842    6972    7004    7150    7182 
   Size:        597      32    3034      32     130      32     146      32

Cuts at:   7182    8334 
   Size:       1152

  Fragments arranged by size: 

                3034    1414    1152     769     597     577     259     146
                 130      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    5253    5575    8334 
   Size:       5253     322    2759

  Fragments arranged by size: 

                5253    2759     322
 
HindIII A'AGCT_T

Cuts at:      0     335    5450    8334 
   Size:        335    5115    2884

  Fragments arranged by size: 

                5115    2884     335
 
KasI G'GCGC_C

Cuts at:      0     117     697    6194    7474    7675    8185    8334 
   Size:        117     580    5497    1280     201     510     149

  Fragments arranged by size: 

                5497    1280     580     510     201     149     117
 
KpnI G_GTAC'C

Cuts at:      0    2754    8334 
   Size:       2754    5580
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3851    5713    6660    8334 
   Size:       3851    1862     947    1674

  Fragments arranged by size: 

                3851    1862    1674     947
 
MscI TGG'CCA

Cuts at:      0      22    1172    2680    3167    3464    6950    7876    8140 
   Size:         22    1150    1508     487     297    3486     926     264

Cuts at:   8140    8334 
   Size:        194

  Fragments arranged by size: 

                3486    1508    1150     926     487     297     264     194
                  22
 
MslI CAynn'nnrTG

Cuts at:      0     842    2198    2858    3610    4342    4774    5188    5941 
   Size:        842    1356     660     752     732     432     414     753

Cuts at:   5941    6401    7135    7255    7330    8323    8334 
   Size:        460     734     120      75     993      11

  Fragments arranged by size: 

                1356     993     842     753     752     734     732     660
                 460     432     414     120      75      11
 
MspA1I CmG'CkG

Cuts at:      0      99     291     575     694    1115    1598    1802    1943 
   Size:         99     192     284     119     421     483     204     141

Cuts at:   1943    2996    3011    3060    3108    3272    4016    4263    4525 
   Size:       1053      15      49      48     164     744     247     262

Cuts at:   4525    4564    4743    4758    5657    6245    6266    6413    6515 
   Size:         39     179      15     899     588      21     147     102

Cuts at:   6515    6560    6660    6676    7104    7512    7568    7833    8157 
   Size:         45     100      16     428     408      56     265     324

Cuts at:   8157    8334 
   Size:        177

  Fragments arranged by size: 

                1053     899     744     588     483     428     421     408
                 324     284     265     262     247     204     192     179
                 177     164     147     141     119     102     100      99
                  56      49      48      45      39      21      16      15
                  15
 
NaeI GCC'GGC

Cuts at:      0     864    1603    2593    3382    3424    6697    7849    7949 
   Size:        864     739     990     789      42    3273    1152     100

Cuts at:   7949    8334 
   Size:        385

  Fragments arranged by size: 

                3273    1152     990     864     789     739     385     100
                  42
 
NarI GG'CG_CC

Cuts at:      0     118     698    6195    7475    7676    8186    8334 
   Size:        118     580    5497    1280     201     510     148

  Fragments arranged by size: 

                5497    1280     580     510     201     148     118
 
NcoI C'CATG_G

Cuts at:      0      23    1178    1215    3168    4340    5186    5484    6431 
   Size:         23    1155      37    1953    1172     846     298     947

Cuts at:   6431    8036    8334 
   Size:       1605     298

  Fragments arranged by size: 

                1953    1605    1172    1155     947     846     298     298
                  37      23
 
NdeI CA'TA_TG

Cuts at:      0    4515    8334 
   Size:       4515    3819
 
NgoMIV G'CCGG_C

Cuts at:      0     862    1601    2591    3380    3422    6695    7847    7947 
   Size:        862     739     990     789      42    3273    1152     100

Cuts at:   7947    8334 
   Size:        387

  Fragments arranged by size: 

                3273    1152     990     862     789     739     387     100
                  42
 
NspI r_CATG'y

Cuts at:      0    1294    1549    2588    5832    6290    6965    7022    8328 
   Size:       1294     255    1039    3244     458     675      57    1306

Cuts at:   8328    8334 
   Size:          6

  Fragments arranged by size: 

                3244    1306    1294    1039     675     458     255      57
                   6
 
PasI CC'CwG_GG

Cuts at:      0       6      67     561     759    1890    2155    2242    4395 
   Size:          6      61     494     198    1131     265      87    2153

Cuts at:   4395    5388    6153    6471    8027    8334 
   Size:        993     765     318    1556     307

  Fragments arranged by size: 

                2153    1556    1131     993     765     494     318     307
                 265     198      87      61       6
 
PciI A'CATG_T

Cuts at:      0    1545    6286    6961    8334 
   Size:       1545    4741     675    1373

  Fragments arranged by size: 

                4741    1545    1373     675
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     797    1179    3460    4194    4341    4367    4641    5334 
   Size:        797     382    2281     734     147      26     274     693

Cuts at:   5334    5520    8334 
   Size:        186    2814

  Fragments arranged by size: 

                2814    2281     797     734     693     382     274     186
                 147      26
 
PfoI T'CCnGG_A

Cuts at:      0     227    2315    3848    5054    5111    6742    6914    7443 
   Size:        227    2088    1533    1206      57    1631     172     529

Cuts at:   7443    8334 
   Size:        891

  Fragments arranged by size: 

                2088    1631    1533    1206     891     529     227     172
                  57
 
PmlI CAC'GTG

Cuts at:      0    1281    3149    8334 
   Size:       1281    1868    5185

  Fragments arranged by size: 

                5185    1868    1281
 
PpuMI rG'GwC_Cy

Cuts at:      0     496     663     744     810    1719    1772    1879    2462 
   Size:        496     167      81      66     909      53     107     583

Cuts at:   2462    2535    3450    3752    4590    5708    6136    6689    6826 
   Size:         73     915     302     838    1118     428     553     137

Cuts at:   6826    8032    8334 
   Size:       1206     302

  Fragments arranged by size: 

                1206    1118     915     909     838     583     553     496
                 428     302     302     167     137     107      81      73
                  66      53
 
PshAI GACnn'nnGTC

Cuts at:      0     815    8334 
   Size:        815    7519
 
PspOMI G'GGCC_C

Cuts at:      0      71     988    1329    2923    3321    4029    4227    4476 
   Size:         71     917     341    1594     398     708     198     249

Cuts at:   4476    5302    7423    7669    8023    8095    8167    8334 
   Size:        826    2121     246     354      72      72     167

  Fragments arranged by size: 

                2121    1594     917     826     708     398     354     341
                 249     246     198     167      72      72      71
 
PstI C_TGCA'G

Cuts at:      0     515    2150    2650    2849    3295    4083    5593    6068 
   Size:        515    1635     500     199     446     788    1510     475

Cuts at:   6068    6902    7416    8334 
   Size:        834     514     918

  Fragments arranged by size: 

                1635    1510     918     834     788     515     514     500
                 475     446     199
 
PvuII CAG'CTG

Cuts at:      0     694    1802    4263    4743    4758    6413    6676    8157 
   Size:        694    1108    2461     480      15    1655     263    1481

Cuts at:   8157    8334 
   Size:        177

  Fragments arranged by size: 

                2461    1655    1481    1108     694     480     263     177
                  15
 
RsrII CG'GwC_CG

Cuts at:      0    3873    8334 
   Size:       3873    4461
 
SacI G_AGCT'C

Cuts at:      0     959    4913    8334 
   Size:        959    3954    3421

  Fragments arranged by size: 

                3954    3421     959
 
SacII CC_GC'GG

Cuts at:      0    3061    7513    7569    8334 
   Size:       3061    4452      56     765

  Fragments arranged by size: 

                4452    3061     765      56
 
SalI G'TCGA_C

Cuts at:      0    5573    8334 
   Size:       5573    2761
 
SanDI GG'GwC_CC

Cuts at:      0     663    1719    1772    2462    3450    3752    8032    8334 
   Size:        663    1056      53     690     988     302    4280     302

  Fragments arranged by size: 

                4280    1056     988     690     663     302     302      53
            
 
SapI GCTCTTCn'nnn_

Cuts at:      0    7714    8334 
   Size:       7714     620
 
SbfI CC_TGCA'GG

Cuts at:      0     515    6902    8334 
   Size:        515    6387    1432

  Fragments arranged by size: 

                6387    1432     515
 
ScaI AGT'ACT

Cuts at:      0    6784    8236    8334 
   Size:       6784    1452      98

  Fragments arranged by size: 

                6784    1452      98
 
SexAI A'CCwGG_T

Cuts at:      0    5246    5476    8334 
   Size:       5246     230    2858

  Fragments arranged by size: 

                5246    2858     230
 
SfcI C'TryA_G

Cuts at:      0     150     511    2146    2646    2845    2905    3291    3733 
   Size:        150     361    1635     500     199      60     386     442

Cuts at:   3733    4079    5589    6064    6898    7412    8334 
   Size:        346    1510     475     834     514     922

  Fragments arranged by size: 

                1635    1510     922     834     514     500     475     442
                 386     361     346     199     150      60
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1846    6310    8334 
   Size:       1846    4464    2024

  Fragments arranged by size: 

                4464    2024    1846
 
SfoI GGC'GCC

Cuts at:      0     119     699    6196    7476    7677    8187    8334 
   Size:        119     580    5497    1280     201     510     147

  Fragments arranged by size: 

                5497    1280     580     510     201     147     119
 
SmaI CCC'GGG

Cuts at:      0      76     428    1717    3254    3280    3559    3750    4035 
   Size:         76     352    1289    1537      26     279     191     285

Cuts at:   4035    7429    8334 
   Size:       3394     905

  Fragments arranged by size: 

                3394    1537    1289     905     352     285     279     191
                  76      26
 
SmlI C'TyrA_G

Cuts at:      0    7618    8107    8334 
   Size:       7618     489     227

  Fragments arranged by size: 

                7618     489     227
 
SphI G_CATG'C

Cuts at:      0    2588    8334 
   Size:       2588    5746
 
SrfI GCCC'GGGC

Cuts at:      0      76    8334 
   Size:         76    8258
 
StuI AGG'CCT

Cuts at:      0     998    1840    1929    2017    4688    8334 
   Size:        998     842      89      88    2671    3646

  Fragments arranged by size: 

                3646    2671     998     842      89      88
 
StyI C'CwwG_G

Cuts at:      0      23      41    1178    1215    1333    1455    1740    1778 
   Size:         23      18    1137      37     118     122     285      38

Cuts at:   1778    1924    3168    3213    4340    5186    5484    6431    6629 
   Size:        146    1244      45    1127     846     298     947     198

Cuts at:   6629    6764    7892    8036    8334 
   Size:        135    1128     144     298

  Fragments arranged by size: 

                1244    1137    1128    1127     947     846     298     298
                 285     198     146     144     135     122     118      45
                  38      37      23      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     626     751     871    1420    1461    1816    2041    2103 
   Size:        626     125     120     549      41     355     225      62

Cuts at:   2103    2545    2728    2943    4186    4764    6804    8193    8334 
   Size:        442     183     215    1243     578    2040    1389     141

  Fragments arranged by size: 

                2040    1389    1243     626     578     549     442     355
                 225     215     183     141     125     120      62      41
            
 
TatI w'GTAC_w

Cuts at:      0    1691    2787    4415    6782    7368    8234    8334 
   Size:       1691    1096    1628    2367     586     866     100

  Fragments arranged by size: 

                2367    1691    1628    1096     866     586     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     223     255     306     338    1216    1248    1408    1440 
   Size:        223      32      51      32     878      32     160      32

Cuts at:   1440    1854    1886    2788    2820    4980    5012    5577    5609 
   Size:        414      32     902      32    2160      32     565      32

Cuts at:   5609    5809    5841    6910    6942    7132    7164    8048    8080 
   Size:        200      32    1069      32     190      32     884      32

Cuts at:   8080    8135    8167    8334 
   Size:         55      32     167

  Fragments arranged by size: 

                2160    1069     902     884     878     565     414     223
                 200     190     167     160      55      51      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     769    8334 
   Size:        769    7565
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4545    6235    7821    8334 
   Size:       4545    1690    1586     513

  Fragments arranged by size: 

                4545    1690    1586     513
 
XhoI C'TCGA_G

Cuts at:      0    8107    8334 
   Size:       8107     227
 
XmaI C'CCGG_G

Cuts at:      0      74     426    1715    3252    3278    3557    3748    4033 
   Size:         74     352    1289    1537      26     279     191     285

Cuts at:   4033    7427    8334 
   Size:       3394     907

  Fragments arranged by size: 

                3394    1537    1289     907     352     285     279     191
                  74      26
 
XmnI GAAnn'nnTTC

Cuts at:      0    7088    8334 
   Size:       7088    1246
 
ZraI GAC'GTC

Cuts at:      0    5578    8334 
   Size:       5578    2756
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
     PstI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AgeI     AloI     AscI     AseI    AsiSI
     BaeI    BciVI     BclI    BfrBI     BmtI    BsaBI    BsiWI    BspHI
   BssHII    BstBI  BstZ17I     ClaI     DraI    EcoRI    EcoRV     FalI
     FspI    FspAI     HpaI     MfeI     MluI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI
     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI     XbaI
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