Restriction map for pF1KSDA0798
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfaI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       FauI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI         1493  GCGG                 CCGC(-3/-1)
AlwI          267  GGATC                GGATC(4/5)
AlwNI          67  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           75  GGGCCC               GGGCC^C
BanII          75  GGGCCC               G[GA]GC[CT]^C
BbsI          805  GTCTTC               GAAGAC(2/6)
BcnI           99  CCGGG                CC^[GC]GG
BglII         116  AGATCT               A^GATCT
BmyI           75  GGGCCC               G[AGT]GC[ACT]^C
BpuAI         805  GTCTTC               GAAGAC(2/6)
BsaAI         613  CACGTA               [CT]AC^GT[GA]
BsaBI         253  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI         651  GTCTC                GTCTC(1/5)
Bsp1286I       75  GGGCCC               G[AGT]GC[ACT]^C
BspMI         128  ACCTGC               ACCTGC(4/8)
BstYI         116  AGATCT               [GA]^GATC[CT]
DdeI          653  CTCAG                C^T[ACGT]AG
DraII          75  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          196  CCATGG               C^C[GA][CT]GG
EcoO109I       75  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         758  GAATTC               G^AATTC
EcoT22I       323  ATGCAT               ATGCA^T
HaeIII         76  GGCC                 GG^CC
HapII          99  CCGG                 C^CGG
HincII        430  GTCAAC               GT[CT]^[GA]AC
HindII        430  GTCAAC               GT[CT]^[GA]AC
HpaII          99  CCGG                 C^CGG
MaeII         614  ACGT                 A^CGT
MamI          253  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          116  AGATCT               [GA]^GATC[CT]
MspI           99  CCGG                 C^CGG
MwoI          890  GCTTCCCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           99  CCGGG                CC^[GC]GG
NcoI          196  CCATGG               C^CATGG
NlaIV          75  GGGCCC               GG[ACGT]^[ACGT]CC
NsiI          323  ATGCAT               ATGCA^T
PleI         1407  GAGTC                GAGTC(4/5)
TfiI          227  GAATC                G^A[AT]TC
XhoII         116  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BbvI           66  GCAGC                GCAGC(8/12)
              526  GCAGC               
BsgI         1458  GTGCAG               GTGCAG(16/14)
             1626  GTGCAG              
Cfr13I         75  GGGCC                G^G[ACGT]CC
               76  GGCCC               
EcoT14I       196  CCATGG               C^C[AT][AT]GG
             1736  CCAAGG              
Fnu4HI         66  GCAGC                GC^[ACGT]GC
              526  GCAGC               
HinfI         227  GAATC                G^A[ACGT]TC
             1407  GAGTC               
ItaI           66  GCAGC                GC^[ACGT]GC
              526  GCAGC               
MslI         1115  CATATATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1283  CATATCTATG          
PstI          262  CTGCAG               CTGCA^G
              524  CTGCAG              
Sau96I         75  GGGCC                G^G[ACGT]CC
               76  GGCCC               
SphI          989  GCATGC               GCATG^C
             1325  GCATGC              
StyI          196  CCATGG               C^C[AT][AT]GG
             1736  CCAAGG              
TaqI          225  TCGA                 T^CGA
             1166  TCGA                
TseI           66  GCAGC                G^C[AT]GC
              526  GCAGC               
TthHB8I       225  TCGA                 T^CGA
             1166  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        Asp700I     
BclI        BpmI        BsaJI       BseRI       Bsp1407I    
BspHI       BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       DpnII       EarI        
EcoRII      FbaI        FokI        HphI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        NdeI        NdeII       NlaIII      NspI        
RcaI        RsaI        SapI        Sau3AI      ScrFI       
SfaNI       SfcI        SspBI       Tru9I       Tsp509I     
TspRI       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com