Restriction map for pF1KSDA0797
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BcgI        BglI        BglII       BlnI        BsaHI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsoBI       BspDI       BspEI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       Bsu36I      ClaI        
CpoI        DraIII      DrdI        DsaI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
Hin1I       HpaI        KasI        KpnI        KspI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MvnI        NarI        NcoI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AflII        2721  CTTAAG               C^TTAAG
AflIII        326  ACATGT               A^C[GA][CT]GT
AlwNI        3081  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI         1321  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII        3045  GGGCTC               G[GA]GC[CT]^C
BbvCI        1057  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2915  GTATCC               GTATCC(6/5)
BclI          108  TGATCA               T^GATCA
BfrI         2721  CTTAAG               C^TTAAG
BlpI         1548  GCTGAGC              GC^T[ACGT]AGC
BmyI         3045  GGGCTC               G[AGT]GC[ACT]^C
Bpu1102I     1548  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2239  CACGTA               [CT]AC^GT[GA]
BsaBI        2512  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI           5  CGGCCG               CG[GA][CT]^CG
BsmFI         148  GGGAC                GGGAC(10/14)
BsmI         1160  GCATTC               GAATGC(1/-1)
Bsp1286I     3045  GGGCTC               G[AGT]GC[ACT]^C
Bsp1407I     1783  TGTACA               T^GTACA
BspLU11I      326  ACATGT               A^CATGT
BspMI        2015  ACCTGC               ACCTGC(4/8)
BsrBI          15  GAGCGG               CCGCTC(-3/-3)
BsrFI           7  GCCGGC               [GA]^CCGG[CT]
BsrGI        1783  TGTACA               T^GTACA
Bst1107I     1346  GTATAC               GTA^TAC
BstXI        2423  CCAGTGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1346  GTATAC               GTA^TAC
CelII        1548  GCTGAGC              GC^T[ACGT]AGC
CfoI           26  GCGC                 GCG^C
Cfr10I          7  GCCGGC               [GA]^CCGG[CT]
EaeI            5  CGGCCG               [CT]^GGCC[GA]
EagI            5  CGGCCG               C^GGCCG
EclXI           5  CGGCCG               C^GGCCG
Eco52I          5  CGGCCG               C^GGCCG
EcoT14I       540  CCAAGG               C^C[AT][AT]GG
FbaI          108  TGATCA               T^GATCA
HgaI         2107  GACGC                GACGC(5/10)
HhaI           26  GCGC                 GCG^C
HinP1I         26  GCGC                 G^CGC
HincII       2791  GTCGAC               GT[CT]^[GA]AC
HindII       2791  GTCGAC               GT[CT]^[GA]AC
HindIII        50  AAGCTT               A^AGCTT
MamI         2512  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI            7  GCCGGC               GCC^GGC
NgoMIV          7  GCCGGC               G^CCGGC
PflFI        1321  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1093  CCATCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2012  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         785  GACAGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2859  CTGCAG               CTGCA^G
SalI         2791  GTCGAC               G^TCGAC
SspBI        1783  TGTACA               T^GTACA
StyI          540  CCAAGG               C^C[AT][AT]GG
Tth111I      1321  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1093  CCATCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AseI          514  ATTAAT               AT^TAAT
             1699  ATTAAT              
AsnI          514  ATTAAT               AT^TAAT
             1699  ATTAAT              
Asp700I       841  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3020  GAAGTTTTTC          
BcnI         2234  CCCGG                CC^[GC]GG
             2320  CCCGG               
BfaI           59  CTAG                 C^TAG
             1420  CTAG                
BspHI         111  TCATGA               T^CATGA
             2175  TCATGA              
BsrDI        1193  CATTGC               GCAATG(2/0)
             2494  CATTGC              
Cac8I           7  GCCGGC               GC[ACGT]^[ACGT]GC
             1062  GCCTGC              
DraII        2012  AGGACCT              [GA]G^G[ACGT]CC[CT]
             2940  AGGCCCT             
EcoO109I     2012  AGGACCT              [GA]G^G[ACGT]CC[CT]
             2940  AGGCCCT             
HphI          244  GGTGA                GGTGA(8/7)
             2755  TCACC               
MaeI           59  CTAG                 C^TAG
             1420  CTAG                
MfeI          314  CAATTG               C^AATTG
              851  CAATTG              
MunI          314  CAATTG               C^AATTG
              851  CAATTG              
NciI         2234  CCCGG                CC^[GC]GG
             2320  CCCGG               
NdeI          263  CATATG               CA^TATG
             2559  CATATG              
NspI          326  ACATGT               [GA]CATG^[CT]
             1075  GCATGT              
PshBI         514  ATTAAT               AT^TAAT
             1699  ATTAAT              
RcaI          111  TCATGA               T^CATGA
             2175  TCATGA              
SmlI         1274  CTCAAG               C^T[CT][GA]AG
             2721  CTTAAG              
XmnI          841  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3020  GAAGTTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaII       BbsI        BbvI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       DraI        
EarI        EcoRI       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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