Restriction map for pF1KSDA0785
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AccI        AclI        AflII       AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        AspEI       AviII       AvrII       BciVI       
BclI        BfaI        BfrI        BlnI        BsaBI       
BsiWI       BsmI        BspDI       BspHI       BsrDI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRV       FbaI        FspI        
HincII      HindII      HindIII     HpaI        KspI        
MaeI        MamI        MfeI        MluI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tru9I       
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         847  GACGTC               GACGT^C
Acc65I       1244  GGTACC               G^GTACC
AccIII        169  TCCGGA               T^CCGGA
AcsI          220  GAATTC               [GA]^AATT[CT]
Alw44I        856  GTGCAC               G^TGCAC
AlwNI         370  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         856  GTGCAC               G^TGCAC
ApoI          220  GAATTC               [GA]^AATT[CT]
Asp718I      1244  GGTACC               G^GTACC
AspI         1516  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1867  GGATCC               G^GATCC
BbeI          661  GGCGCC               GGCGC^C
BbrPI        1918  CACGTG               CAC^GTG
BbvCI        1546  GCTGAGG              CCTCAGC(-5/-2)
BcgI          815  GCAAGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI        1918  CACGTG               [CT]AC^GT[GA]
BsaWI         169  TCCGGA               [AT]^CCGG[AT]
BseAI         169  TCCGGA               T^CCGGA
BsgI         1276  CTGCAC               GTGCAG(16/14)
Bsp1407I      986  TGTACA               T^GTACA
BspEI         169  TCCGGA               T^CCGGA
BsrBI        1465  GAGCGG               CCGCTC(-3/-3)
BsrGI         986  TGTACA               T^GTACA
DraIII        573  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        2004  CCTGGACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         220  GAATTC               G^AATTC
EcoT22I       111  ATGCAT               ATGCA^T
FseI          339  GGCCGGCC             GGCCGG^CC
KasI          661  GGCGCC               G^GCGCC
KpnI         1244  GGTACC               GGTAC^C
MroI          169  TCCGGA               T^CCGGA
NarI          661  GGCGCC               GG^CGCC
NcoI          680  CCATGG               C^CATGG
NsiI          111  ATGCAT               ATGCA^T
PflFI        1516  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1918  CACGTG               CAC^GTG
PmlI         1918  CACGTG               CAC^GTG
SexAI         574  ACCTGGT              A^CC[AT]GGT
SfiI          330  GGCCGTCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          661  GGCGCC               GGC^GCC
SmaI          313  CCCGGG               CCC^GGG
SspBI         986  TGTACA               T^GTACA
StuI          902  AGGCCT               AGG^CCT
Tsp509I       221  AATT                 ^AATT
Tth111I      1516  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         313  CCCGGG               C^CCGGG
XmaI          313  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII        596  ACATGT               A^C[GA][CT]GT
             1397  ACATGT              
Asp700I        78  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              699  GAAGACCTTC          
BglI          331  GCCGTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1658  GCCAGACAGGC         
BglII        1556  AGATCT               A^GATCT
             1649  AGATCT              
BlpI         1329  GCTCAGC              GC^T[ACGT]AGC
             1713  GCTGAGC             
Bpu1102I     1329  GCTCAGC              GC^T[ACGT]AGC
             1713  GCTGAGC             
BsaI          733  GAGACC               GGTCTC(1/5)
             1312  GAGACC              
BspLU11I      596  ACATGT               A^CATGT
             1397  ACATGT              
BsrI          951  ACTGG                ACTGG(1/-1)
             1823  ACTGG               
BstXI        1253  CCATCAGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1415  CCACCTTCCTGG        
CelII        1329  GCTCAGC              GC^T[ACGT]AGC
             1713  GCTGAGC             
DraII        1505  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             2000  GGGGCCT             
EcoO109I     1505  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             2000  GGGGCCT             
MaeIII        631  GTCAC                ^GT[ACGT]AC
             1831  GTGAC               
PleI         1318  GACTC                GAGTC(4/5)
              298  GAGTC               
ScaI          959  AGTACT               AGT^ACT
             1820  AGTACT              
SmlI         1090  CTCAAG               C^T[CT][GA]AG
             1762  CTCAAG              
SphI         1031  GCATGC               GCATG^C
             1692  GCATGC              
TfiI          612  GAATC                G^A[AT]TC
             1482  GAATC               
Tsp45I        631  GTCAC                ^GT[GC]AC
             1831  GTGAC               
XmnI           78  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              699  GAAGACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        TspRI       TthHB8I     
Van91I      XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com