Restriction map for pF1KSDA0785
 (Linear) (Six-base) MAPSORT of: KIAA0785.seq  Check: 1309  from: 1  to: 2082



KIAA0785.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1206    1464    1920    2007    2082 
   Size:       1206     258     456      87      75

  Fragments arranged by size: 

                1206     456     258      87      75
 
AatII G_ACGT'C

Cuts at:      0     851    2082 
   Size:        851    1231
 
Acc65I G'GTAC_C

Cuts at:      0    1244    2082 
   Size:       1244     838
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     310     478     574     718    2082 
   Size:         58     252     168      96     144    1364

  Fragments arranged by size: 

                1364     252     168     144      96      58
 
AflIII A'CryG_T

Cuts at:      0     596    1397    2082 
   Size:        596     801     685

  Fragments arranged by size: 

                 801     685     596
 
AleI CACnn'nnGTG

Cuts at:      0     745    2082 
   Size:        745    1337
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1087    1119    2005    2037    2082 
   Size:       1087      32     886      32      45

  Fragments arranged by size: 

                1087     886      45      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     375    2082 
   Size:        375    1707
 
ApaLI G'TGCA_C

Cuts at:      0     856    2082 
   Size:        856    1226
 
ApoI r'AATT_y

Cuts at:      0     220    2082 
   Size:        220    1862
 
AvaI C'yCGr_G

Cuts at:      0     214     295     313    1387    1570    1975    2082 
   Size:        214      81      18    1074     183     405     107

  Fragments arranged by size: 

                1074     405     214     183     107      81      18
 
BamHI G'GATC_C

Cuts at:      0    1867    2082 
   Size:       1867     215
 
BanI G'GyrC_C

Cuts at:      0     661     711    1244    1566    2082 
   Size:        661      50     533     322     516

  Fragments arranged by size: 

                 661     533     516     322      50
 
BanII G_rGCy'C

Cuts at:      0      25     440     688    1199    1331    2082 
   Size:         25     415     248     511     132     751

  Fragments arranged by size: 

                 751     511     415     248     132      25
 
BbeI G_GCGC'C

Cuts at:      0     665    2082 
   Size:        665    1417
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     466     706     753     783    1760    2082 
   Size:        466     240      47      30     977     322

  Fragments arranged by size: 

                 977     466     322     240      47      30
 
BbvCI CC'TCA_GC

Cuts at:      0    1547    2082 
   Size:       1547     535
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     804     838    2082 
   Size:        804      34    1244

  Fragments arranged by size: 

                1244     804      34
 
BfrBI ATG'CAT

Cuts at:      0     113    2082 
   Size:        113    1969
 
BglI GCCn_nnn'nGGC

Cuts at:      0     337    1664    2082 
   Size:        337    1327     418

  Fragments arranged by size: 

                1327     418     337
 
BglII A'GATC_T

Cuts at:      0    1556    1649    2082 
   Size:       1556      93     433

  Fragments arranged by size: 

                1556     433      93
 
BlpI GC'TnA_GC

Cuts at:      0    1330    1714    2082 
   Size:       1330     384     368

  Fragments arranged by size: 

                1330     384     368
 
Bme1580I G_kGCm'C

Cuts at:      0     716     860    1571    2082 
   Size:        716     144     711     511

  Fragments arranged by size: 

                 716     711     511     144
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1335    2082 
   Size:       1335     747
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     220    1177    1224    2082 
   Size:         37     183     957      47     858

  Fragments arranged by size: 

                 957     858     183      47      37
 
Bpu10I CC'TnA_GC

Cuts at:      0    1547    1853    2082 
   Size:       1547     306     229

  Fragments arranged by size: 

                1547     306     229
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1075    1747    2082 
   Size:       1075     672     335

  Fragments arranged by size: 

                1075     672     335
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     727    1306    2082 
   Size:        727     579     776

  Fragments arranged by size: 

                 776     727     579
 
BsaAI yAC'GTr

Cuts at:      0    1920    2082 
   Size:       1920     162
 
BsaHI Gr'CG_yC

Cuts at:      0     662     848    1384    2039    2082 
   Size:        662     186     536     655      43

  Fragments arranged by size: 

                 662     655     536     186      43
 
BsaWI w'CCGG_w

Cuts at:      0     169    2082 
   Size:        169    1913
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     406     436    1036    1066    1087    1117    2005    2035 
   Size:        406      30     600      30      21      30     888      30

Cuts at:   2035    2082 
   Size:         47

  Fragments arranged by size: 

                 888     600     406      47      30      30      30      30
                  21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     618    1588    1723    2082 
   Size:        618     970     135     359

  Fragments arranged by size: 

                 970     618     359     135
 
BseYI C'CCAG_C

Cuts at:      0     305     390     417     487     921    2082 
   Size:        305      85      27      70     434    1161

  Fragments arranged by size: 

                1161     434     305      85      70      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1261    2082 
   Size:       1261     821
 
BsiEI CG_ry'CG

Cuts at:      0     383     467    1543    2082 
   Size:        383      84    1076     539

  Fragments arranged by size: 

                1076     539     383      84
 
BsiHKAI G_wGCw'C

Cuts at:      0      25     752     838     860    1069    1199    1331    2082 
   Size:         25     727      86      22     209     130     132     751

  Fragments arranged by size: 

                 751     727     209     132     130      86      25      22
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1056    1375    1864    2082 
   Size:       1056     319     489     218

  Fragments arranged by size: 

                1056     489     319     218
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     440     688     716     752     838     860    1069 
   Size:         25     415     248      28      36      86      22     209

Cuts at:   1069    1199    1331    1571    2082 
   Size:        130     132     240     511

  Fragments arranged by size: 

                 511     415     248     240     209     132     130      86
                  36      28      25      22
 
BspEI T'CCGG_A

Cuts at:      0     169    2082 
   Size:        169    1913
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1206    1464    1920    2007    2082 
   Size:       1206     258     456      87      75

  Fragments arranged by size: 

                1206     456     258      87      75
 
BsrBI CCG'CTC

Cuts at:      0    1467    2082 
   Size:       1467     615
 
BsrFI r'CCGG_y

Cuts at:      0     340     889    1538    1542    1901    2082 
   Size:        340     549     649       4     359     181

  Fragments arranged by size: 

                 649     549     359     340     181       4
 
BsrGI T'GTAC_A

Cuts at:      0     986    2082 
   Size:        986    1096
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1260    1422    2082 
   Size:       1260     162     660

  Fragments arranged by size: 

                1260     660     162
 
BstYI r'GATC_y

Cuts at:      0    1556    1649    1867    2082 
   Size:       1556      93     218     215

  Fragments arranged by size: 

                1556     218     215      93
 
BtgI C'CryG_G

Cuts at:      0     345     680     977    2082 
   Size:        345     335     297    1105

  Fragments arranged by size: 

                1105     345     335     297
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1095    1335    2082 
   Size:       1095     240     747

  Fragments arranged by size: 

                1095     747     240
 
BtsI GCAGTG_nn'

Cuts at:      0     127     860    1026    2082 
   Size:        127     733     166    1056

  Fragments arranged by size: 

                1056     733     166     127
 
DraIII CAC_nnn'GTG

Cuts at:      0     578    2082 
   Size:        578    1504
 
EaeI y'GGCC_r

Cuts at:      0     329     342     380     464     563     638     980    1073 
   Size:        329      13      38      84      99      75     342      93

Cuts at:   1073    1220    1337    1412    1540    1643    1835    2082 
   Size:        147     117      75     128     103     192     247

  Fragments arranged by size: 

                 342     329     247     192     147     128     117     103
                  99      93      84      75      75      38      13
 
EagI C'GGCC_G

Cuts at:      0     380     464    1540    2082 
   Size:        380      84    1076     542

  Fragments arranged by size: 

                1076     542     380      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     713    1490    1679    1795    1867    2082 
   Size:        713     777     189     116      72     215

  Fragments arranged by size: 

                 777     713     215     189     116      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     745    1446    1860    1984    2082 
   Size:        745     701     414     124      98

  Fragments arranged by size: 

                 745     701     414     124      98
 
EcoICRI GAG'CTC

Cuts at:      0      23    1197    1329    2082 
   Size:         23    1174     132     753

  Fragments arranged by size: 

                1174     753     132      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37      58     220     310     478     574     718    1177 
   Size:         37      21     162      90     168      96     144     459

Cuts at:   1177    1224    2082 
   Size:         47     858

  Fragments arranged by size: 

                 858     459     168     162     144      96      90      47
                  37      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2008    2082 
   Size:       2008      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1506    2001    2082 
   Size:       1506     495      81

  Fragments arranged by size: 

                1506     495      81
 
EcoRI G'AATT_C

Cuts at:      0     220    2082 
   Size:        220    1862
 
FseI GG_CCGG'CC

Cuts at:      0     344    2082 
   Size:        344    1738
 
HaeII r_GCGC'y

Cuts at:      0     665     785     931    1089    1892    2082 
   Size:        665     120     146     158     803     190

  Fragments arranged by size: 

                 803     665     190     158     146     120
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     597     629    1216    1248    2082 
   Size:        597      32     587      32     834

  Fragments arranged by size: 

                 834     597     587      32      32
 
KasI G'GCGC_C

Cuts at:      0     661    2082 
   Size:        661    1421
 
KpnI G_GTAC'C

Cuts at:      0    1248    2082 
   Size:       1248     834
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    2082 
   Size:        206    1876
 
MscI TGG'CCA

Cuts at:      0     982    1075    1339    1414    2082 
   Size:        982      93     264      75     668

  Fragments arranged by size: 

                 982     668     264      93      75
 
MslI CAynn'nnrTG

Cuts at:      0     148     595     745     988    1036    1258    2082 
   Size:        148     447     150     243      48     222     824

  Fragments arranged by size: 

                 824     447     243     222     150     148      48
 
MspA1I CmG'CkG

Cuts at:      0     692    1272    2045    2082 
   Size:        692     580     773      37

  Fragments arranged by size: 

                 773     692     580      37
 
NaeI GCC'GGC

Cuts at:      0     342     891    1544    2082 
   Size:        342     549     653     538

  Fragments arranged by size: 

                 653     549     538     342
 
NarI GG'CG_CC

Cuts at:      0     662    2082 
   Size:        662    1420
 
NcoI C'CATG_G

Cuts at:      0     680    2082 
   Size:        680    1402
 
NgoMIV G'CCGG_C

Cuts at:      0     340     889    1542    2082 
   Size:        340     549     653     540

  Fragments arranged by size: 

                 653     549     540     340
 
NsiI A_TGCA'T

Cuts at:      0     115    2082 
   Size:        115    1967
 
NspI r_CATG'y

Cuts at:      0     600     864     993    1035    1284    1401    1641    1696 
   Size:        600     264     129      42     249     117     240      55

Cuts at:   1696    2082 
   Size:        386

  Fragments arranged by size: 

                 600     386     264     249     240     129     117      55
                  42
 
PciI A'CATG_T

Cuts at:      0     596    1397    2082 
   Size:        596     801     685

  Fragments arranged by size: 

                 801     685     596
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     651    1169    1704    2082 
   Size:        651     518     535     378

  Fragments arranged by size: 

                 651     535     518     378
 
PfoI T'CCnGG_A

Cuts at:      0     539    1421    2082 
   Size:        539     882     661

  Fragments arranged by size: 

                 882     661     539
 
PmlI CAC'GTG

Cuts at:      0    1920    2082 
   Size:       1920     162
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1087    1119    2005    2037    2082 
   Size:       1087      32     886      32      45

  Fragments arranged by size: 

                1087     886      45      32      32
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     610     642    2082 
   Size:        610      32    1440

  Fragments arranged by size: 

                1440     610      32
 
PstI C_TGCA'G

Cuts at:      0     122    1085    1205    1817    2082 
   Size:        122     963     120     612     265

  Fragments arranged by size: 

                 963     612     265     122     120
 
SacI G_AGCT'C

Cuts at:      0      25    1199    1331    2082 
   Size:         25    1174     132     751

  Fragments arranged by size: 

                1174     751     132      25
 
ScaI AGT'ACT

Cuts at:      0     961    1822    2082 
   Size:        961     861     260

  Fragments arranged by size: 

                 961     861     260
 
SexAI A'CCwGG_T

Cuts at:      0     574    2082 
   Size:        574    1508
 
SfcI C'TryA_G

Cuts at:      0     118    1081    1201    1813    2082 
   Size:        118     963     120     612     269

  Fragments arranged by size: 

                 963     612     269     120     118
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     337    2082 
   Size:        337    1745
 
SfoI GGC'GCC

Cuts at:      0     663    2082 
   Size:        663    1419
 
SmaI CCC'GGG

Cuts at:      0     315    2082 
   Size:        315    1767
 
SmlI C'TyrA_G

Cuts at:      0    1090    1762    2082 
   Size:       1090     672     320

  Fragments arranged by size: 

                1090     672     320
 
SphI G_CATG'C

Cuts at:      0    1035    1696    2082 
   Size:       1035     661     386

  Fragments arranged by size: 

                1035     661     386
 
SrfI GCCC'GGGC

Cuts at:      0     315    2082 
   Size:        315    1767
 
StuI AGG'CCT

Cuts at:      0     904    2082 
   Size:        904    1178
 
StyI C'CwwG_G

Cuts at:      0     509     680     785    1322    1340    1355    1502    1805 
   Size:        509     171     105     537      18      15     147     303

Cuts at:   1805    2027    2082 
   Size:        222      55

  Fragments arranged by size: 

                 537     509     303     222     171     147     105      55
                  18      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1330    2082 
   Size:       1330     752
 
TatI w'GTAC_w

Cuts at:      0     497     959     986    1820    2082 
   Size:        497     462      27     834     262

  Fragments arranged by size: 

                 834     497     462     262      27
 
Tth111I GACn'n_nGTC

Cuts at:      0    1519    2082 
   Size:       1519     563
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1185    1257    1509    1738    2082 
   Size:       1185      72     252     229     344

  Fragments arranged by size: 

                1185     344     252     229      72
 
XmaI C'CCGG_G

Cuts at:      0     313    2082 
   Size:        313    1769
 
XmnI GAAnn'nnTTC

Cuts at:      0      82     703    2082 
   Size:         82     621    1379

  Fragments arranged by size: 

                1379     621      82
 
ZraI GAC'GTC

Cuts at:      0     849    2082 
   Size:        849    1233
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AleI     AloI    AlwNI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrFI    BsrGI    BstXI    BstYI     BtgI    BtgZI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FseI    HaeII    Hin4I     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PciI    PflMI     PfoI     PmlI     PpiI     PsrI
     PstI     SacI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI
     SmlI     SphI     SrfI     StuI     StyI    TaqII     TatI  Tth111I
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AhdI     AlfI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BciVI     BclI    BmgBI
     BmrI     BmtI    BsaBI    BsiWI     BsmI    BspHI    BsrDI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
     DraI     DrdI    EcoRV     FalI     FspI    FspAI   HincII  HindIII
     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI     PacI
     PasI     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     SgfI
    SgrAI    SnaBI     SpeI     SspI     SwaI     TstI     XbaI     XhoI
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