Restriction map for pF1KSDA0781
 (Linear) (Six-base) MAPSORT of: KIAA0781.seq  Check: 8637  from: 1  to: 2778



KIAA0781.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371    1722    1789    2155    2380    2778 
   Size:        371    1351      67     366     225     398

  Fragments arranged by size: 

                1351     398     371     366     225      67
 
AfeI AGC'GCT

Cuts at:      0    2559    2778 
   Size:       2559     219
 
AflII C'TTAA_G

Cuts at:      0    1603    2778 
   Size:       1603    1175
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1109    2778 
   Size:       1109    1669
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2132    2164    2778 
   Size:       2132      32     614

  Fragments arranged by size: 

                2132     614      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     900    1210    1326    1692    1812    2022    2037    2133 
   Size:        900     310     116     366     120     210      15      96

Cuts at:   2133    2250    2319    2403    2509    2778 
   Size:        117      69      84     106     269

  Fragments arranged by size: 

                 900     366     310     269     210     120     117     116
                 106      96      84      69      15
 
ApaLI G'TGCA_C

Cuts at:      0     415    2778 
   Size:        415    2363
 
ApoI r'AATT_y

Cuts at:      0     308     364     492    1499    1829    2778 
   Size:        308      56     128    1007     330     949

  Fragments arranged by size: 

                1007     949     330     308     128      56
 
AvaI C'yCGr_G

Cuts at:      0      18    2423    2778 
   Size:         18    2405     355

  Fragments arranged by size: 

                2405     355      18
 
BamHI G'GATC_C

Cuts at:      0    2745    2778 
   Size:       2745      33
 
BanI G'GyrC_C

Cuts at:      0     113    1896    2778 
   Size:        113    1783     882

  Fragments arranged by size: 

                1783     882     113
 
BanII G_rGCy'C

Cuts at:      0     642    1118    1699    2227    2326    2616    2778 
   Size:        642     476     581     528      99     290     162

  Fragments arranged by size: 

                 642     581     528     476     290     162      99
 
BbeI G_GCGC'C

Cuts at:      0    1900    2778 
   Size:       1900     878
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1397    1595    2778 
   Size:       1397     198    1183

  Fragments arranged by size: 

                1397    1183     198
 
BbvCI CC'TCA_GC

Cuts at:      0    1910    2371    2778 
   Size:       1910     461     407

  Fragments arranged by size: 

                1910     461     407
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       9      43    2778 
   Size:          9      34    2735

  Fragments arranged by size: 

                2735      34       9
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2609    2778 
   Size:       2609     169
 
BfrBI ATG'CAT

Cuts at:      0    1131    2778 
   Size:       1131    1647
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1411    1687    2778 
   Size:       1411     276    1091

  Fragments arranged by size: 

                1411    1091     276
 
BlpI GC'TnA_GC

Cuts at:      0    1082    2778 
   Size:       1082    1696
 
Bme1580I G_kGCm'C

Cuts at:      0      73     419    2778 
   Size:         73     346    2359

  Fragments arranged by size: 

                2359     346      73
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1027    1114    2025    2778 
   Size:       1027      87     911     753

  Fragments arranged by size: 

                1027     911     753      87
 
BmtI G_CTAG'C

Cuts at:      0    1931    2778 
   Size:       1931     847
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2358    2691    2778 
   Size:       2358     333      87

  Fragments arranged by size: 

                2358     333      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     621    1387    1712    1900    2035    2044    2134    2353 
   Size:        621     766     325     188     135       9      90     219

Cuts at:   2353    2661    2778 
   Size:        308     117

  Fragments arranged by size: 

                 766     621     325     308     219     188     135     117
                  90       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    1910    2320    2371    2778 
   Size:       1910     410      51     407

  Fragments arranged by size: 

                1910     410     407      51
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    2143    2778 
   Size:        202    1941     635

  Fragments arranged by size: 

                1941     635     202
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253     828    1339    1659    1730    2020    2778 
   Size:        253     575     511     320      71     290     758

  Fragments arranged by size: 

                 758     575     511     320     290     253      71
 
BsaHI Gr'CG_yC

Cuts at:      0    1897    2778 
   Size:       1897     881
 
BsaWI w'CCGG_w

Cuts at:      0     116     701    1875    2416    2778 
   Size:        116     585    1174     541     362

  Fragments arranged by size: 

                1174     585     541     362     116
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1507    1537    2017    2047    2132    2162    2778 
   Size:       1507      30     480      30      85      30     616

  Fragments arranged by size: 

                1507     616     480      85      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1156    1898    2423    2426    2778 
   Size:       1156     742     525       3     352

  Fragments arranged by size: 

                1156     742     525     352       3
 
BseYI C'CCAG_C

Cuts at:      0      75     105    1330    1677    2000    2059    2312    2514 
   Size:         75      30    1225     347     323      59     253     202

Cuts at:   2514    2615    2778 
   Size:        101     163

  Fragments arranged by size: 

                1225     347     323     253     202     163     101      75
                  59      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1870    2778 
   Size:       1870     908
 
BsiHKAI G_wGCw'C

Cuts at:      0     419     642     737    1699    2227    2778 
   Size:        419     223      95     962     528     551

  Fragments arranged by size: 

                 962     551     528     419     223      95
 
BsmI GAATG_Cn'

Cuts at:      0    1131    1231    2778 
   Size:       1131     100    1547

  Fragments arranged by size: 

                1547    1131     100
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1434    1977    2778 
   Size:       1434     543     801

  Fragments arranged by size: 

                1434     801     543
 
Bsp1286I G_dGCh'C

Cuts at:      0      73     419     642     737    1118    1699    2227    2326 
   Size:         73     346     223      95     381     581     528      99

Cuts at:   2326    2616    2778 
   Size:        290     162

  Fragments arranged by size: 

                 581     528     381     346     290     223     162      99
                  95      73
 
BspEI T'CCGG_A

Cuts at:      0     701    2778 
   Size:        701    2077
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     885    2045    2778 
   Size:        885    1160     733

  Fragments arranged by size: 

                1160     885     733
 
BsrBI CCG'CTC

Cuts at:      0    1399    2778 
   Size:       1399    1379
 
BsrDI GCAATG_nn'

Cuts at:      0    1076    2778 
   Size:       1076    1702
 
BsrFI r'CCGG_y

Cuts at:      0      39     335    1899    2778 
   Size:         39     296    1564     879

  Fragments arranged by size: 

                1564     879     296      39
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     900    2133    2778 
   Size:        900    1233     645

  Fragments arranged by size: 

                1233     900     645
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1473    2778 
   Size:       1473    1305
 
BstYI r'GATC_y

Cuts at:      0    2745    2778 
   Size:       2745      33
 
Bsu36I CC'TnA_GG

Cuts at:      0    1940    2778 
   Size:       1940     838
 
BtgI C'CryG_G

Cuts at:      0    2011    2113    2778 
   Size:       2011     102     665

  Fragments arranged by size: 

                2011     665     102
 
BtsI GCAGTG_nn'

Cuts at:      0     179    1324    2778 
   Size:        179    1145    1454

  Fragments arranged by size: 

                1454    1145     179
 
ClaI AT'CG_AT

Cuts at:      0     152    2778 
   Size:        152    2626
 
DraI TTT'AAA

Cuts at:      0     501    2778 
   Size:        501    2277
 
EaeI y'GGCC_r

Cuts at:      0     333    1053    2778 
   Size:        333     720    1725

  Fragments arranged by size: 

                1725     720     333
 
EarI CTCTTCn'nnn_

Cuts at:      0     668    1238    2778 
   Size:        668     570    1540

  Fragments arranged by size: 

                1540     668     570
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      25    1237    2778 
   Size:         25    1212    1541

  Fragments arranged by size: 

                1541    1212      25
 
EcoICRI GAG'CTC

Cuts at:      0     640    1697    2225    2778 
   Size:        640    1057     528     553

  Fragments arranged by size: 

                1057     640     553     528
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     621    1387    1712    1722    1789    1900    2035 
   Size:        371     250     766     325      10      67     111     135

Cuts at:   2035    2044    2134    2155    2353    2380    2661    2778 
   Size:          9      90      21     198      27     281     117

  Fragments arranged by size: 

                 766     371     325     281     250     198     135     117
                 111      90      67      27      21      10       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1171    1490    1774    2778 
   Size:       1171     319     284    1004

  Fragments arranged by size: 

                1171    1004     319     284
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1572    1920    2292    2670    2778 
   Size:       1572     348     372     378     108

  Fragments arranged by size: 

                1572     378     372     348     108
 
EcoRI G'AATT_C

Cuts at:      0    1829    2778 
   Size:       1829     949
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     105    2181    2579    2778 
   Size:        105    2076     398     199

  Fragments arranged by size: 

                2076     398     199     105
 
HaeII r_GCGC'y

Cuts at:      0    1008    1900    2561    2778 
   Size:       1008     892     661     217

  Fragments arranged by size: 

                1008     892     661     217
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     827     859    1148    1180    1507    1539    1543    1575 
   Size:        827      32     289      32     327      32       4      32

Cuts at:   1575    2778 
   Size:       1203

  Fragments arranged by size: 

                1203     827     327     289      32      32      32      32
                   4
 
KasI G'GCGC_C

Cuts at:      0    1896    2778 
   Size:       1896     882
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     385    1205    2778 
   Size:        385     820    1573

  Fragments arranged by size: 

                1573     820     385
 
MspA1I CmG'CkG

Cuts at:      0     165     591    1433    1934    2004    2115    2133    2250 
   Size:        165     426     842     501      70     111      18     117

Cuts at:   2250    2778 
   Size:        528

  Fragments arranged by size: 

                 842     528     501     426     165     117     111      70
                  18
 
NaeI GCC'GGC

Cuts at:      0    1901    2778 
   Size:       1901     877
 
NarI GG'CG_CC

Cuts at:      0    1897    2778 
   Size:       1897     881
 
NgoMIV G'CCGG_C

Cuts at:      0    1899    2778 
   Size:       1899     879
 
NheI G'CTAG_C

Cuts at:      0    1927    2778 
   Size:       1927     851
 
NsiI A_TGCA'T

Cuts at:      0    1133    2778 
   Size:       1133    1645
 
PasI CC'CwG_GG

Cuts at:      0    1776    2778 
   Size:       1776    1002
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1210    2778 
   Size:       1210    1568
 
PfoI T'CCnGG_A

Cuts at:      0    1916    2330    2778 
   Size:       1916     414     448

  Fragments arranged by size: 

                1916     448     414
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2132    2164    2778 
   Size:       2132      32     614

  Fragments arranged by size: 

                2132     614      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1572    1920    2778 
   Size:       1572     348     858

  Fragments arranged by size: 

                1572     858     348
 
PstI C_TGCA'G

Cuts at:      0    2042    2138    2192    2778 
   Size:       2042      96      54     586

  Fragments arranged by size: 

                2042     586      96      54
 
PvuII CAG'CTG

Cuts at:      0     165     591    1934    2004    2133    2250    2778 
   Size:        165     426    1343      70     129     117     528

  Fragments arranged by size: 

                1343     528     426     165     129     117      70
 
RsrII CG'GwC_CG

Cuts at:      0      42    2778 
   Size:         42    2736
 
SacI G_AGCT'C

Cuts at:      0     642    1699    2227    2778 
   Size:        642    1057     528     551

  Fragments arranged by size: 

                1057     642     551     528
 
SacII CC_GC'GG

Cuts at:      0    2116    2778 
   Size:       2116     662
 
SfcI C'TryA_G

Cuts at:      0    2038    2134    2188    2452    2778 
   Size:       2038      96      54     264     326

  Fragments arranged by size: 

                2038     326     264      96      54
 
SfoI GGC'GCC

Cuts at:      0    1898    2778 
   Size:       1898     880
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1899    2778 
   Size:       1899     879
 
SmaI CCC'GGG

Cuts at:      0    2425    2778 
   Size:       2425     353
 
SmlI C'TyrA_G

Cuts at:      0     181    1603    2122    2778 
   Size:        181    1422     519     656

  Fragments arranged by size: 

                1422     656     519     181
 
StuI AGG'CCT

Cuts at:      0    1192    2194    2778 
   Size:       1192    1002     584

  Fragments arranged by size: 

                1192    1002     584
 
StyI C'CwwG_G

Cuts at:      0     918    1097    2778 
   Size:        918     179    1681

  Fragments arranged by size: 

                1681     918     179
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     672    1789    2778 
   Size:        672    1117     989

  Fragments arranged by size: 

                1117     989     672
 
TatI w'GTAC_w

Cuts at:      0     201    2778 
   Size:        201    2577
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1231    1263    2403    2435    2778 
   Size:       1231      32    1140      32     343

  Fragments arranged by size: 

                1231    1140     343      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1833    2778 
   Size:       1833     945
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     132    1338    2778 
   Size:        132    1206    1440

  Fragments arranged by size: 

                1440    1206     132
 
XmaI C'CCGG_G

Cuts at:      0    2423    2778 
   Size:       2423     355
 
XmnI GAAnn'nnTTC

Cuts at:      0    1501    2778 
   Size:       1501    1277
 

 Enzymes that do cut:

     AcuI     AfeI    AflII     AlfI     AloI    AlwNI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     ClaI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I     KasI     MmeI
   MspA1I     NaeI     NarI   NgoMIV     NheI     NsiI     PasI    PflMI
     PfoI     PpiI    PpuMI     PstI    PvuII    RsrII     SacI    SacII
     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BclI
    BglII    BmgBI    BsaAI    BsaBI    BsiEI    BsiWI    BspHI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI    CspCI   DraIII
     DrdI     EagI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     MslI     NcoI     NdeI
     NotI     NruI     NspI     PacI     PciI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XhoI     ZraI
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