Restriction map for pF1KSDA0780
 (Linear) (Six-base) MAPSORT of: KIAA0780.seq  Check: 7543  from: 1  to: 3141



KIAA0780.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1305    2817    3141 
   Size:       1305    1512     324

  Fragments arranged by size: 

                1512    1305     324
 
AccI GT'mk_AC

Cuts at:      0    1132    1471    3141 
   Size:       1132     339    1670

  Fragments arranged by size: 

                1670    1132     339
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39    1082    1268    2815    3141 
   Size:         39    1043     186    1547     326

  Fragments arranged by size: 

                1547    1043     326     186      39
 
AflII C'TTAA_G

Cuts at:      0    1365    2293    3141 
   Size:       1365     928     848

  Fragments arranged by size: 

                1365     928     848
 
AflIII A'CryG_T

Cuts at:      0    2891    3141 
   Size:       2891     250
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2593    3141 
   Size:       2593     548
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2178    3141 
   Size:       2178     963
 
ApaI G_GGCC'C

Cuts at:      0    2322    2806    3141 
   Size:       2322     484     335

  Fragments arranged by size: 

                2322     484     335
 
ApaLI G'TGCA_C

Cuts at:      0     921    3141 
   Size:        921    2220
 
ApoI r'AATT_y

Cuts at:      0     802     873     973    1326    3033    3141 
   Size:        802      71     100     353    1707     108

  Fragments arranged by size: 

                1707     802     353     108     100      71
 
AseI AT'TA_AT

Cuts at:      0     413     596    3095    3141 
   Size:        413     183    2499      46

  Fragments arranged by size: 

                2499     413     183      46
 
AvaI C'yCGr_G

Cuts at:      0    1489    3141 
   Size:       1489    1652
 
BamHI G'GATC_C

Cuts at:      0    2917    3141 
   Size:       2917     224
 
BanI G'GyrC_C

Cuts at:      0     390    1712    1770    3141 
   Size:        390    1322      58    1371

  Fragments arranged by size: 

                1371    1322     390      58
 
BanII G_rGCy'C

Cuts at:      0     123     620    2322    2806    3141 
   Size:        123     497    1702     484     335

  Fragments arranged by size: 

                1702     497     484     335     123
 
BbeI G_GCGC'C

Cuts at:      0    1716    1774    3141 
   Size:       1716      58    1367

  Fragments arranged by size: 

                1716    1367      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     556     581    1391    3141 
   Size:        556      25     810    1750

  Fragments arranged by size: 

                1750     810     556      25
 
BbvCI CC'TCA_GC

Cuts at:      0    1264    2813    3141 
   Size:       1264    1549     328

  Fragments arranged by size: 

                1549    1264     328
 
BclI T'GATC_A

Cuts at:      0     809    1029    3141 
   Size:        809     220    2112

  Fragments arranged by size: 

                2112     809     220
 
BfrBI ATG'CAT

Cuts at:      0     712    1417    2429    3141 
   Size:        712     705    1012     712

  Fragments arranged by size: 

                1012     712     712     705
 
BglII A'GATC_T

Cuts at:      0    1741    2207    3141 
   Size:       1741     466     934

  Fragments arranged by size: 

                1741     934     466
 
BlpI GC'TnA_GC

Cuts at:      0    1889    3141 
   Size:       1889    1252
 
Bme1580I G_kGCm'C

Cuts at:      0     925    2232    2322    2523    2806    3141 
   Size:        925    1307      90     201     283     335

  Fragments arranged by size: 

                1307     925     335     283     201      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1637    1667    3141 
   Size:       1637      30    1474

  Fragments arranged by size: 

                1637    1474      30
 
BmtI G_CTAG'C

Cuts at:      0    1307    3141 
   Size:       1307    1834
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1557    3141 
   Size:       1557    1584
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     818    1031    1718    2483    3141 
   Size:        818     213     687     765     658

  Fragments arranged by size: 

                 818     765     687     658     213
 
Bpu10I CC'TnA_GC

Cuts at:      0    1264    2813    3141 
   Size:       1264    1549     328

  Fragments arranged by size: 

                1549    1264     328
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     659    1213    3141 
   Size:        659     554    1928

  Fragments arranged by size: 

                1928     659     554
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2463    3141 
   Size:       2463     678
 
BsaHI Gr'CG_yC

Cuts at:      0    1713    1771    3141 
   Size:       1713      58    1370

  Fragments arranged by size: 

                1713    1370      58
 
BsaWI w'CCGG_w

Cuts at:      0     642    3141 
   Size:        642    2499
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     349     379    2810    2840    3141 
   Size:        349      30    2431      30     301

  Fragments arranged by size: 

                2431     349     301      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85    1098    1101    1825    2297    3141 
   Size:         85    1013       3     724     472     844

  Fragments arranged by size: 

                1013     844     724     472      85       3
 
BseYI C'CCAG_C

Cuts at:      0      34    1632    2530    2799    3065    3141 
   Size:         34    1598     898     269     266      76

  Fragments arranged by size: 

                1598     898     269     266      76      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     881    1926    3141 
   Size:        425     456    1045    1215

  Fragments arranged by size: 

                1215    1045     456     425
 
BsiEI CG_ry'CG

Cuts at:      0    2490    3141 
   Size:       2490     651
 
BsiHKAI G_wGCw'C

Cuts at:      0     925    2292    3141 
   Size:        925    1367     849

  Fragments arranged by size: 

                1367     925     849
 
BsiWI C'GTAC_G

Cuts at:      0    2166    3141 
   Size:       2166     975
 
BsmI GAATG_Cn'

Cuts at:      0    2266    3141 
   Size:       2266     875
 
Bsp1286I G_dGCh'C

Cuts at:      0     123     620     925    2232    2292    2322    2523    2806 
   Size:        123     497     305    1307      60      30     201     283

Cuts at:   2806    3141 
   Size:        335

  Fragments arranged by size: 

                1307     497     335     305     283     201     123      60
                  30
 
BspEI T'CCGG_A

Cuts at:      0     642    3141 
   Size:        642    2499
 
BspHI T'CATG_A

Cuts at:      0     806    2202    3141 
   Size:        806    1396     939

  Fragments arranged by size: 

                1396     939     806
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1305    2817    3031    3141 
   Size:       1305    1512     214     110

  Fragments arranged by size: 

                1512    1305     214     110
 
BsrDI GCAATG_nn'

Cuts at:      0    1986    2936    3141 
   Size:       1986     950     205

  Fragments arranged by size: 

                1986     950     205
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2435    3141 
   Size:       2435     706
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     796    1609    2530    3141 
   Size:        796     813     921     611

  Fragments arranged by size: 

                 921     813     796     611
 
BstYI r'GATC_y

Cuts at:      0    1741    2207    2917    3141 
   Size:       1741     466     710     224

  Fragments arranged by size: 

                1741     710     466     224
 
Bsu36I CC'TnA_GG

Cuts at:      0     155    1148    1793    2768    3141 
   Size:        155     993     645     975     373

  Fragments arranged by size: 

                 993     975     645     373     155
 
BtgI C'CryG_G

Cuts at:      0      65    1602    2639    3141 
   Size:         65    1537    1037     502

  Fragments arranged by size: 

                1537    1037     502      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     292    2693    3141 
   Size:        292    2401     448

  Fragments arranged by size: 

                2401     448     292
 
BtsI GCAGTG_nn'

Cuts at:      0    1262    3141 
   Size:       1262    1879
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2818    2853    3141 
   Size:       2818      35     288

  Fragments arranged by size: 

                2818     288      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1933    3141 
   Size:       1933    1208
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1231    3141 
   Size:       1231    1910
 
EaeI y'GGCC_r

Cuts at:      0       8     305    1519    1913    3141 
   Size:          8     297    1214     394    1228

  Fragments arranged by size: 

                1228    1214     394     297       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     485    1157    1286    1577    2117    2929    2957    3141 
   Size:        485     672     129     291     540     812      28     184

  Fragments arranged by size: 

                 812     672     540     485     291     184     129      28
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     818    1031    1082    1268    1718    2483    2815 
   Size:         39     779     213      51     186     450     765     332

Cuts at:   2815    3141 
   Size:        326

  Fragments arranged by size: 

                 779     765     450     332     326     213     186      51
                  39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1140    2122    3141 
   Size:       1140     982    1019

  Fragments arranged by size: 

                1140    1019     982
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1197    3020    3141 
   Size:       1197    1823     121

  Fragments arranged by size: 

                1823    1197     121
 
EcoRI G'AATT_C

Cuts at:      0     802    3033    3141 
   Size:        802    2231     108

  Fragments arranged by size: 

                2231     802     108
 
EcoRV GAT'ATC

Cuts at:      0     960     991    2775    3141 
   Size:        960      31    1784     366

  Fragments arranged by size: 

                1784     960     366      31
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1448    3141 
   Size:       1448    1693
 
HaeII r_GCGC'y

Cuts at:      0    1716    1774    3141 
   Size:       1716      58    1367

  Fragments arranged by size: 

                1716    1367      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     607     639    1156    1188    1786    1818    2456    2488 
   Size:        607      32     517      32     598      32     638      32

Cuts at:   2488    2579    2611    2728    2760    2810    2842    2930    2962 
   Size:         91      32     117      32      50      32      88      32

Cuts at:   2962    3141 
   Size:        179

  Fragments arranged by size: 

                 638     607     598     517     179     117      91      88
                  50      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1571    2598    3141 
   Size:       1571    1027     543

  Fragments arranged by size: 

                1571    1027     543
 
HindIII A'AGCT_T

Cuts at:      0    1113    1409    3141 
   Size:       1113     296    1732

  Fragments arranged by size: 

                1732    1113     296
 
HpaI GTT'AAC

Cuts at:      0    1571    3141 
   Size:       1571    1570
 
KasI G'GCGC_C

Cuts at:      0    1712    1770    3141 
   Size:       1712      58    1371

  Fragments arranged by size: 

                1712    1371      58
 
MscI TGG'CCA

Cuts at:      0     307    1521    1915    3141 
   Size:        307    1214     394    1226

  Fragments arranged by size: 

                1226    1214     394     307
 
MslI CAynn'nnrTG

Cuts at:      0    1607    2638    3141 
   Size:       1607    1031     503

  Fragments arranged by size: 

                1607    1031     503
 
MspA1I CmG'CkG

Cuts at:      0      38     303    1361    1636    1888    3141 
   Size:         38     265    1058     275     252    1253

  Fragments arranged by size: 

                1253    1058     275     265     252      38
 
NarI GG'CG_CC

Cuts at:      0    1713    1771    3141 
   Size:       1713      58    1370

  Fragments arranged by size: 

                1713    1370      58
 
NcoI C'CATG_G

Cuts at:      0      65    1602    3141 
   Size:         65    1537    1539

  Fragments arranged by size: 

                1539    1537      65
 
NdeI CA'TA_TG

Cuts at:      0     822    3141 
   Size:        822    2319
 
NheI G'CTAG_C

Cuts at:      0    1303    3141 
   Size:       1303    1838
 
NsiI A_TGCA'T

Cuts at:      0     714    1419    2431    3141 
   Size:        714     705    1012     710

  Fragments arranged by size: 

                1012     714     710     705
 
NspI r_CATG'y

Cuts at:      0     546    2582    2895    3141 
   Size:        546    2036     313     246

  Fragments arranged by size: 

                2036     546     313     246
 
PciI A'CATG_T

Cuts at:      0    2891    3141 
   Size:       2891     250
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     626    3141 
   Size:        626    2515
 
PfoI T'CCnGG_A

Cuts at:      0      77    3141 
   Size:         77    3064
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1684    1716    3141 
   Size:       1684      32    1425

  Fragments arranged by size: 

                1684    1425      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1197    3020    3141 
   Size:       1197    1823     121

  Fragments arranged by size: 

                1823    1197     121
 
PspOMI G'GGCC_C

Cuts at:      0    2318    2802    3141 
   Size:       2318     484     339

  Fragments arranged by size: 

                2318     484     339
 
PstI C_TGCA'G

Cuts at:      0    1082    2440    3141 
   Size:       1082    1358     701

  Fragments arranged by size: 

                1358    1082     701
 
PvuII CAG'CTG

Cuts at:      0      38     303    1361    1888    3141 
   Size:         38     265    1058     527    1253

  Fragments arranged by size: 

                1253    1058     527     265      38
 
ScaI AGT'ACT

Cuts at:      0     367    3141 
   Size:        367    2774
 
SexAI A'CCwGG_T

Cuts at:      0    2897    3141 
   Size:       2897     244
 
SfcI C'TryA_G

Cuts at:      0     588    1078    2436    3141 
   Size:        588     490    1358     705

  Fragments arranged by size: 

                1358     705     588     490
 
SfoI GGC'GCC

Cuts at:      0    1714    1772    3141 
   Size:       1714      58    1369

  Fragments arranged by size: 

                1714    1369      58
 
SmlI C'TyrA_G

Cuts at:      0     674    1228    1365    2293    3141 
   Size:        674     554     137     928     848

  Fragments arranged by size: 

                 928     848     674     554     137
 
SspI AAT'ATT

Cuts at:      0     322    2098    2869    2883    3141 
   Size:        322    1776     771      14     258

  Fragments arranged by size: 

                1776     771     322     258      14
 
StuI AGG'CCT

Cuts at:      0    1146    1731    3141 
   Size:       1146     585    1410

  Fragments arranged by size: 

                1410    1146     585
 
StyI C'CwwG_G

Cuts at:      0      65     699    1602    2615    3141 
   Size:         65     634     903    1013     526

  Fragments arranged by size: 

                1013     903     634     526      65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2121    3141 
   Size:       2121    1020
 
TatI w'GTAC_w

Cuts at:      0     257     325     365    1369    3141 
   Size:        257      68      40    1004    1772

  Fragments arranged by size: 

                1772    1004     257      68      40
 
Tth111I GACn'n_nGTC

Cuts at:      0    1213    1869    3141 
   Size:       1213     656    1272

  Fragments arranged by size: 

                1272    1213     656
 
XmnI GAAnn'nnTTC

Cuts at:      0     292    3141 
   Size:        292    2849
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AhdI     AlfI     ApaI
    ApaLI     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NsiI     NspI     PciI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     ScaI    SexAI
     SfcI     SfoI     SmlI     SspI     StuI     StyI    TaqII     TatI
  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AleI     AloI    AlwNI
     AscI    AsiSI    AvrII     BaeI     BcgI    BciVI     BglI    BmgBI
    BsaAI    BsaBI    BsmBI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I     ClaI     DraI     EagI     EciI  EcoICRI
     FseI     FspI    FspAI     KpnI     MfeI     MluI     MmeI     NaeI
   NgoMIV     NotI     NruI     PacI     PasI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI
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