Restriction map for pF1KSDA0776
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BglI        
BlnI        BlpI        BmyI        Bpu1102I    BsaBI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmBI       Bsp1286I    BspDI       BspEI       BspHI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        Eam1105I    Eco47III    EcoO109I    EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
StyI        SwaI        Tth111I     Van91I      XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         1789  GTAGAC               GT^[AC][GT]AC
AcyI            7  GACGCC               G[GA]^CG[CT]C
AflIII        210  ACATGT               A^C[GA][CT]GT
AocI         1480  CCTGAGG              CC^T[ACGT]AGG
AseI          256  ATTAAT               AT^TAAT
AsnI          256  ATTAAT               AT^TAAT
Asp700I       136  GAAGTAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1540  CTCGAG               C^[CT]CG[GA]G
AvaII         264  GGACC                G^G[AT]CC
BciVI         566  GTATCC               GTATCC(6/5)
BcnI          593  CCCGG                CC^[GC]GG
BglII        1197  AGATCT               A^GATCT
BsaAI         563  CACGTA               [CT]AC^GT[GA]
BsaHI           7  GACGCC               G[GA]^CG[CT]C
BsgI         2056  CTGCAC               GTGCAG(16/14)
BsiEI          35  CGGCCG               CG[GA][CT]^CG
BsmI         1042  GCATTC               GAATGC(1/-1)
BsoBI        1540  CTCGAG               C^[CT]CG[GA]G
BspLU11I      210  ACATGT               A^CATGT
BspMI        1029  GCAGGT               ACCTGC(4/8)
BsrBI          85  GAGCGG               CCGCTC(-3/-3)
BstEII        408  GGTCACC              G^GT[ACGT]ACC
BstPI         408  GGTCACC              G^GT[ACGT]ACC
BstXI        2106  CCATGCACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1197  AGATCT               [GA]^GATC[CT]
Bsu36I       1480  CCTGAGG              CC^T[ACGT]AGG
CfoI           52  GCGC                 GCG^C
Cfr13I        264  GGACC                G^G[ACGT]CC
DsaI          570  CCGTGG               C^C[GA][CT]GG
EaeI           35  CGGCCG               [CT]^GGCC[GA]
EagI           35  CGGCCG               C^GGCCG
EclXI          35  CGGCCG               C^GGCCG
Eco52I         35  CGGCCG               C^GGCCG
Eco81I       1480  CCTGAGG              CC^T[ACGT]AGG
EcoNI         776  CCTTTTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       408  GGTCACC              G^GT[ACGT]ACC
EcoRI         609  GAATTC               G^AATTC
EcoRV        1738  GATATC               GAT^ATC
FauI           48  GCGGG                CCCGC(4/6)
HapII         594  CCGG                 C^CGG
HhaI           52  GCGC                 GCG^C
Hin1I           7  GACGCC               G[GA]^CG[CT]C
HinP1I         52  GCGC                 G^CGC
HpaII         594  CCGG                 C^CGG
MflI         1197  AGATCT               [GA]^GATC[CT]
MspI          594  CCGG                 C^CGG
NciI          593  CCCGG                CC^[GC]GG
PaeR7I       1540  CTCGAG               C^TCGAG
PshBI         256  ATTAAT               AT^TAAT
PvuII         602  CAGCTG               CAG^CTG
Sau96I        264  GGACC                G^G[ACGT]CC
StuI         1055  AGGCCT               AGG^CCT
XbaI          798  TCTAGA               T^CTAGA
XhoI         1540  CTCGAG               C^TCGAG
XhoII        1197  AGATCT               [GA]^GATC[CT]
XmnI          136  GAAGTAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AlwNI         833  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1987  CAGATACTG           
BpmI          173  CTCCAG               CTGGAG(16/14)
              747  CTCCAG              
BsiYI          68  CCACGCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              776  CCTTTTTCAGG         
BslI           68  CCACGCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              776  CCTTTTTCAGG         
BsmAI         711  GAGAC                GTCTC(1/5)
             1967  GAGAC               
Bsp1407I     1114  TGTACA               T^GTACA
             1640  TGTACA              
BsrDI        2136  CATTGC               GCAATG(2/0)
             1313  GCAATG              
BsrGI        1114  TGTACA               T^GTACA
             1640  TGTACA              
HgaI            7  GACGC                GACGC(5/10)
              683  GACGC               
HindIII       539  AAGCTT               A^AGCTT
             1940  AAGCTT              
MaeII         564  ACGT                 A^CGT
             1551  ACGT                
MspA1I         46  CAGCGG               C[AC]G^C[GT]G
              602  CAGCTG              
PstI         1799  CTGCAG               CTGCA^G
             1931  CTGCAG              
SmlI          661  CTTGAG               C^T[CT][GA]AG
             1540  CTCGAG              
SphI         2099  GCATGC               GCATG^C
             2172  GCATGC              
SspBI        1114  TGTACA               T^GTACA
             1640  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbsI        BbvI        BfaI        BpuAI       
BsaJI       BseRI       BsmFI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NspI        
RsaI        Sau3AI      ScaI        ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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