Restriction map for pF1KSDA0776
 (Linear) (Six-base) MAPSORT of: KIAA0776.seq  Check: 765  from: 1  to: 2382



KIAA0776.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1020    2382 
   Size:       1020    1362
 
AccI GT'mk_AC

Cuts at:      0    1790    2382 
   Size:       1790     592
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1009    1211    1741    2382 
   Size:       1009     202     530     641

  Fragments arranged by size: 

                1009     641     530     202
 
AflIII A'CryG_T

Cuts at:      0     210    2382 
   Size:        210    2172
 
AlwNI CAG_nnn'CTG

Cuts at:      0     838    1992    2382 
   Size:        838    1154     390

  Fragments arranged by size: 

                1154     838     390
 
ApoI r'AATT_y

Cuts at:      0     609     802    1099    1829    2156    2382 
   Size:        609     193     297     730     327     226

  Fragments arranged by size: 

                 730     609     327     297     226     193
 
AseI AT'TA_AT

Cuts at:      0     257    2382 
   Size:        257    2125
 
AvaI C'yCGr_G

Cuts at:      0    1540    2382 
   Size:       1540     842
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1559    1913    2045    2239    2382 
   Size:       1559     354     132     194     143

  Fragments arranged by size: 

                1559     354     194     143     132
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     577    2382 
   Size:        577    1805
 
BglII A'GATC_T

Cuts at:      0    1197    2382 
   Size:       1197    1185
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2246    2382 
   Size:       2246     136
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     732    2382 
   Size:        158     574    1650

  Fragments arranged by size: 

                1650     574     158
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     682    2382 
   Size:        682    1700
 
BsaAI yAC'GTr

Cuts at:      0     565    2382 
   Size:        565    1817
 
BsaHI Gr'CG_yC

Cuts at:      0       8    2382 
   Size:          8    2374
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     730     760    2382 
   Size:        730      30    1622

  Fragments arranged by size: 

                1622     730      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     536    1313    1316    1498    2382 
   Size:        536     777       3     182     884

  Fragments arranged by size: 

                 884     777     536     182       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2041    2382 
   Size:       2041     341
 
BsiEI CG_ry'CG

Cuts at:      0      38    2382 
   Size:         38    2344
 
BsmI GAATG_Cn'

Cuts at:      0    1042    2382 
   Size:       1042    1340
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1020    2382 
   Size:       1020    1362
 
BsrBI CCG'CTC

Cuts at:      0      87    2382 
   Size:         87    2295
 
BsrDI GCAATG_nn'

Cuts at:      0    1320    2135    2382 
   Size:       1320     815     247

  Fragments arranged by size: 

                1320     815     247
 
BsrGI T'GTAC_A

Cuts at:      0    1114    1640    2382 
   Size:       1114     526     742

  Fragments arranged by size: 

                1114     742     526
 
BstEII G'GTnAC_C

Cuts at:      0     408    2382 
   Size:        408    1974
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2113    2382 
   Size:       2113     269
 
BstYI r'GATC_y

Cuts at:      0    1197    2382 
   Size:       1197    1185
 
Bsu36I CC'TnA_GG

Cuts at:      0    1481    2382 
   Size:       1481     901
 
BtgI C'CryG_G

Cuts at:      0     570    2382 
   Size:        570    1812
 
BtsI GCAGTG_nn'

Cuts at:      0    1290    2382 
   Size:       1290    1092
 
EaeI y'GGCC_r

Cuts at:      0      35    2382 
   Size:         35    2347
 
EagI C'GGCC_G

Cuts at:      0      35    2382 
   Size:         35    2347
 
EarI CTCTTCn'nnn_

Cuts at:      0      11     368    2372    2382 
   Size:         11     357    2004      10

  Fragments arranged by size: 

                2004     357      11      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    2382 
   Size:         19    2363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     732    1009    1211    1741    2382 
   Size:        158     574     277     202     530     641

  Fragments arranged by size: 

                 641     574     530     277     202     158
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     780    2382 
   Size:        780    1602
 
EcoRI G'AATT_C

Cuts at:      0     609    2382 
   Size:        609    1773
 
EcoRV GAT'ATC

Cuts at:      0    1740    2382 
   Size:       1740     642
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1882    2382 
   Size:       1882     500
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     730     762     799     812     831     844    1366    1398 
   Size:        730      32      37      13      19      13     522      32

Cuts at:   1398    2382 
   Size:        984

  Fragments arranged by size: 

                 984     730     522      37      32      32      19      13
                  13
 
HindIII A'AGCT_T

Cuts at:      0     539    1940    2382 
   Size:        539    1401     442

  Fragments arranged by size: 

                1401     539     442
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2022    2382 
   Size:       2022     360
 
MspA1I CmG'CkG

Cuts at:      0      48     604    2382 
   Size:         48     556    1778

  Fragments arranged by size: 

                1778     556      48
 
NspI r_CATG'y

Cuts at:      0     214     319    2103    2176    2382 
   Size:        214     105    1784      73     206

  Fragments arranged by size: 

                1784     214     206     105      73
 
PciI A'CATG_T

Cuts at:      0     210    2382 
   Size:        210    2172
 
PfoI T'CCnGG_A

Cuts at:      0     748    1433    2382 
   Size:        748     685     949

  Fragments arranged by size: 

                 949     748     685
 
PsiI TTA'TAA

Cuts at:      0    1105    2382 
   Size:       1105    1277
 
PstI C_TGCA'G

Cuts at:      0    1803    1935    2382 
   Size:       1803     132     447

  Fragments arranged by size: 

                1803     447     132
 
PvuII CAG'CTG

Cuts at:      0     604    2382 
   Size:        604    1778
 
ScaI AGT'ACT

Cuts at:      0     762    1510    2298    2382 
   Size:        762     748     788      84

  Fragments arranged by size: 

                 788     762     748      84
 
SfcI C'TryA_G

Cuts at:      0     599    1064    1799    1931    2382 
   Size:        599     465     735     132     451

  Fragments arranged by size: 

                 735     599     465     451     132
 
SmlI C'TyrA_G

Cuts at:      0     661    1540    2382 
   Size:        661     879     842

  Fragments arranged by size: 

                 879     842     661
 
SphI G_CATG'C

Cuts at:      0    2103    2176    2382 
   Size:       2103      73     206

  Fragments arranged by size: 

                2103     206      73
 
StuI AGG'CCT

Cuts at:      0    1057    2382 
   Size:       1057    1325
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     756    2382 
   Size:        756    1626
 
TatI w'GTAC_w

Cuts at:      0     760    1114    1231    1325    1508    1640    1732    2296 
   Size:        760     354     117      94     183     132      92     564

Cuts at:   2296    2382 
   Size:         86

  Fragments arranged by size: 

                 760     564     354     183     132     117      94      92
                  86
 
XbaI T'CTAG_A

Cuts at:      0     798    2382 
   Size:        798    1584
 
XhoI C'TCGA_G

Cuts at:      0    1540    2382 
   Size:       1540     842
 
XmnI GAAnn'nnTTC

Cuts at:      0     140    2382 
   Size:        140    2242
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI
     BbsI    BciVI    BglII     BmrI     BpmI    BpuEI    BsaAI    BsaHI
    BsaXI    BseRI     BsgI    BsiEI     BsmI    BspMI    BsrBI    BsrDI
    BsrGI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
    Hin4I  HindIII     MmeI   MspA1I     NspI     PciI     PfoI     PsiI
     PstI    PvuII     ScaI     SfcI     SmlI     SphI     StuI    TaqII
     TatI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaBI    BsaWI    BseYI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BsrFI   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI EcoO109I     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StyI     SwaI     TstI  Tth111I     XcmI     XmaI     ZraI
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