Restriction map for pF1KSDA0758
 (Linear) (Six-base) MAPSORT of: KIAA0758.seq  Check: 4449  from: 1  to: 4038



KIAA0758.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1024    3163    4038 
   Size:       1024    2139     875

  Fragments arranged by size: 

                2139    1024     875
 
AatII G_ACGT'C

Cuts at:      0     431    1679    4038 
   Size:        431    1248    2359

  Fragments arranged by size: 

                2359    1248     431
 
AccI GT'mk_AC

Cuts at:      0      85    4038 
   Size:         85    3953
 
AclI AA'CG_TT

Cuts at:      0    2072    4038 
   Size:       2072    1966
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     481    2266    2825    2859    3832    4038 
   Size:        200     281    1785     559      34     973     206

  Fragments arranged by size: 

                1785     973     559     281     206     200      34
 
AleI CACnn'nnGTG

Cuts at:      0    3571    3712    3901    4038 
   Size:       3571     141     189     137

  Fragments arranged by size: 

                3571     189     141     137
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     553     585     595     627    4038 
   Size:        553      32      10      32    3411

  Fragments arranged by size: 

                3411     553      32      32      10
 
ApoI r'AATT_y

Cuts at:      0     629    1112    1650    2361    4038 
   Size:        629     483     538     711    1677

  Fragments arranged by size: 

                1677     711     629     538     483
 
AvaI C'yCGr_G

Cuts at:      0     386    3455    4038 
   Size:        386    3069     583

  Fragments arranged by size: 

                3069     583     386
 
BamHI G'GATC_C

Cuts at:      0     231    2010    4038 
   Size:        231    1779    2028

  Fragments arranged by size: 

                2028    1779     231
 
BanI G'GyrC_C

Cuts at:      0     443    4038 
   Size:        443    3595
 
BanII G_rGCy'C

Cuts at:      0    1963    2215    4038 
   Size:       1963     252    1823

  Fragments arranged by size: 

                1963    1823     252
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      97     391     423     557    2856    3291    3759    3851 
   Size:         97     294      32     134    2299     435     468      92

Cuts at:   3851    4038 
   Size:        187

  Fragments arranged by size: 

                2299     468     435     294     187     134      97      92
                  32
 
BbvCI CC'TCA_GC

Cuts at:      0    3292    4038 
   Size:       3292     746
 
BclI T'GATC_A

Cuts at:      0    2204    3539    4038 
   Size:       2204    1335     499

  Fragments arranged by size: 

                2204    1335     499
 
BfrBI ATG'CAT

Cuts at:      0     220    1475    4038 
   Size:        220    1255    2563

  Fragments arranged by size: 

                2563    1255     220
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2843    3259    3517    4038 
   Size:       2843     416     258     521

  Fragments arranged by size: 

                2843     521     416     258
 
Bme1580I G_kGCm'C

Cuts at:      0     446    1309    4038 
   Size:        446     863    2729

  Fragments arranged by size: 

                2729     863     446
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1296    2127    3328    3504    4038 
   Size:       1296     831    1201     176     534

  Fragments arranged by size: 

                1296    1201     831     534     176
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2234    4038 
   Size:       2234    1804
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1007    2170    4038 
   Size:       1007    1163    1868

  Fragments arranged by size: 

                1868    1163    1007
 
Bpu10I CC'TnA_GC

Cuts at:      0    2195    2729    3292    4038 
   Size:       2195     534     563     746

  Fragments arranged by size: 

                2195     746     563     534
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     742    1270    3093    4038 
   Size:        742     528    1823     945

  Fragments arranged by size: 

                1823     945     742     528
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3709    4038 
   Size:       3709     329
 
BsaAI yAC'GTr

Cuts at:      0    2382    4038 
   Size:       2382    1656
 
BsaHI Gr'CG_yC

Cuts at:      0     428    1676    4038 
   Size:        428    1248    2362

  Fragments arranged by size: 

                2362    1248     428
 
BsaWI w'CCGG_w

Cuts at:      0     614    4038 
   Size:        614    3424
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     553     583     775     805    4038 
   Size:        553      30     192      30    3233

  Fragments arranged by size: 

                3233     553     192      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1572    1792    2155    4038 
   Size:       1572     220     363    1883

  Fragments arranged by size: 

                1883    1572     363     220
 
BseYI C'CCAG_C

Cuts at:      0    2077    3438    3449    3531    4038 
   Size:       2077    1361      11      82     507

  Fragments arranged by size: 

                2077    1361     507      82      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47    2572    4038 
   Size:         47    2525    1466

  Fragments arranged by size: 

                2525    1466      47
 
BsiHKAI G_wGCw'C

Cuts at:      0     361    2387    4038 
   Size:        361    2026    1651

  Fragments arranged by size: 

                2026    1651     361
 
Bsp1286I G_dGCh'C

Cuts at:      0     361     446    1309    1963    2215    2387    4038 
   Size:        361      85     863     654     252     172    1651

  Fragments arranged by size: 

                1651     863     654     361     252     172      85
 
BspEI T'CCGG_A

Cuts at:      0     614    4038 
   Size:        614    3424
 
BspHI T'CATG_A

Cuts at:      0     108     557    4038 
   Size:        108     449    3481

  Fragments arranged by size: 

                3481     449     108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     345    1024    3163    3359    4038 
   Size:        345     679    2139     196     679

  Fragments arranged by size: 

                2139     679     679     345     196
 
BsrDI GCAATG_nn'

Cuts at:      0     447    2455    2643    3386    4038 
   Size:        447    2008     188     743     652

  Fragments arranged by size: 

                2008     743     652     447     188
 
BsrFI r'CCGG_y

Cuts at:      0    2060    4038 
   Size:       2060    1978
 
BsrGI T'GTAC_A

Cuts at:      0     753    4038 
   Size:        753    3285
 
BssSI C'ACGA_G

Cuts at:      0    1554    4038 
   Size:       1554    2484
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2915    4038 
   Size:       2915    1123
 
BstEII G'GTnAC_C

Cuts at:      0    3120    4038 
   Size:       3120     918
 
BstYI r'GATC_y

Cuts at:      0     231    2010    2250    3006    3587    3807    4038 
   Size:        231    1779     240     756     581     220     231

  Fragments arranged by size: 

                1779     756     581     240     231     231     220
 
Bsu36I CC'TnA_GG

Cuts at:      0    3592    4038 
   Size:       3592     446
 
BtgI C'CryG_G

Cuts at:      0    2696    4038 
   Size:       2696    1342
 
BtsI GCAGTG_nn'

Cuts at:      0    1383    1629    1752    2916    4038 
   Size:       1383     246     123    1164    1122

  Fragments arranged by size: 

                1383    1164    1122     246     123
 
DraIII CAC_nnn'GTG

Cuts at:      0      26    4038 
   Size:         26    4012
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     993    4038 
   Size:        993    3045
 
EaeI y'GGCC_r

Cuts at:      0    1409    4038 
   Size:       1409    2629
 
EarI CTCTTCn'nnn_

Cuts at:      0     125    2140    2298    3625    4038 
   Size:        125    2015     158    1327     413

  Fragments arranged by size: 

                2015    1327     413     158     125
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     561    4038 
   Size:        561    3477
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     481    1007    2170    2266    2825    2859    3832 
   Size:        200     281     526    1163      96     559      34     973

Cuts at:   3832    4038 
   Size:        206

  Fragments arranged by size: 

                1163     973     559     526     281     206     200      96
                  34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     496    1605    2728    4038 
   Size:        496    1109    1123    1310

  Fragments arranged by size: 

                1310    1123    1109     496
 
EcoO109I rG'GnC_Cy

Cuts at:      0     584    3510    3666    4038 
   Size:        584    2926     156     372

  Fragments arranged by size: 

                2926     584     372     156
 
EcoRI G'AATT_C

Cuts at:      0    1650    4038 
   Size:       1650    2388
 
EcoRV GAT'ATC

Cuts at:      0    2736    4038 
   Size:       2736    1302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     615    2436    2740    2880    4038 
   Size:        615    1821     304     140    1158

  Fragments arranged by size: 

                1821    1158     615     304     140
 
HaeII r_GCGC'y

Cuts at:      0     915    4038 
   Size:        915    3123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     750     775     782     807    1268    1300    1548    1580 
   Size:        750      25       7      25     461      32     248      32

Cuts at:   1580    2146    2178    2323    2355    3114    3146    4038 
   Size:        566      32     145      32     759      32     892

  Fragments arranged by size: 

                 892     759     750     566     461     248     145      32
                  32      32      32      32      25      25       7
 
HincII GTy'rAC

Cuts at:      0    1845    3878    4038 
   Size:       1845    2033     160

  Fragments arranged by size: 

                2033    1845     160
 
HindIII A'AGCT_T

Cuts at:      0    3824    4038 
   Size:       3824     214
 
HpaI GTT'AAC

Cuts at:      0    1845    4038 
   Size:       1845    2193
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     911    2007    2374    2657    4038 
   Size:        911    1096     367     283    1381

  Fragments arranged by size: 

                1381    1096     911     367     283
 
MscI TGG'CCA

Cuts at:      0    1411    4038 
   Size:       1411    2627
 
MslI CAynn'nnrTG

Cuts at:      0     113     316    1390    1405    2986    3547    3571    3712 
   Size:        113     203    1074      15    1581     561      24     141

Cuts at:   3712    3901    4038 
   Size:        189     137

  Fragments arranged by size: 

                1581    1074     561     203     189     141     137     113
                  24      15
 
MspA1I CmG'CkG

Cuts at:      0     124    1692    4038 
   Size:        124    1568    2346

  Fragments arranged by size: 

                2346    1568     124
 
NcoI C'CATG_G

Cuts at:      0    2696    4038 
   Size:       2696    1342
 
NdeI CA'TA_TG

Cuts at:      0     693    1808    4038 
   Size:        693    1115    2230

  Fragments arranged by size: 

                2230    1115     693
 
NsiI A_TGCA'T

Cuts at:      0     222    1477    4038 
   Size:        222    1255    2561

  Fragments arranged by size: 

                2561    1255     222
 
NspI r_CATG'y

Cuts at:      0    2006    4038 
   Size:       2006    2032
 
PasI CC'CwG_GG

Cuts at:      0     439    3721    3885    4038 
   Size:        439    3282     164     153

  Fragments arranged by size: 

                3282     439     164     153
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     274    1116    1613    3197    4038 
   Size:        274     842     497    1584     841

  Fragments arranged by size: 

                1584     842     841     497     274
 
PfoI T'CCnGG_A

Cuts at:      0     227    1236    2738    3032    3221    4038 
   Size:        227    1009    1502     294     189     817

  Fragments arranged by size: 

                1502    1009     817     294     227     189
 
PmlI CAC'GTG

Cuts at:      0    2382    4038 
   Size:       2382    1656
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     553     585    4038 
   Size:        553      32    3453

  Fragments arranged by size: 

                3453     553      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     584    3666    4038 
   Size:        584    3082     372

  Fragments arranged by size: 

                3082     584     372
 
PshAI GACnn'nnGTC

Cuts at:      0     325    4038 
   Size:        325    3713
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      61      93     737     769    4038 
   Size:         61      32     644      32    3269

  Fragments arranged by size: 

                3269     644      61      32      32
 
PstI C_TGCA'G

Cuts at:      0     334    1177    1273    4038 
   Size:        334     843      96    2765

  Fragments arranged by size: 

                2765     843     334      96
 
PvuII CAG'CTG

Cuts at:      0     124    4038 
   Size:        124    3914
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2298    3625    4038 
   Size:       2298    1327     413

  Fragments arranged by size: 

                2298    1327     413
 
ScaI AGT'ACT

Cuts at:      0    1586    4038 
   Size:       1586    2452
 
SexAI A'CCwGG_T

Cuts at:      0    3196    4038 
   Size:       3196     842
 
SfcI C'TryA_G

Cuts at:      0     330     579    1173    1269    4038 
   Size:        330     249     594      96    2769

  Fragments arranged by size: 

                2769     594     330     249      96
 
SmaI CCC'GGG

Cuts at:      0    3457    4038 
   Size:       3457     581
 
SmlI C'TyrA_G

Cuts at:      0     721    1285    3072    4038 
   Size:        721     564    1787     966

  Fragments arranged by size: 

                1787     966     721     564
 
SspI AAT'ATT

Cuts at:      0     198    1233    4038 
   Size:        198    1035    2805

  Fragments arranged by size: 

                2805    1035     198
 
StuI AGG'CCT

Cuts at:      0    3597    4038 
   Size:       3597     441
 
StyI C'CwwG_G

Cuts at:      0      10    2406    2696    3506    4038 
   Size:         10    2396     290     810     532

  Fragments arranged by size: 

                2396     810     532     290      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     368     610    2600    2903    3417    3463    4038 
   Size:        368     242    1990     303     514      46     575

  Fragments arranged by size: 

                1990     575     514     368     303     242      46
 
TatI w'GTAC_w

Cuts at:      0     753    1584    4038 
   Size:        753     831    2454

  Fragments arranged by size: 

                2454     831     753
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     153     185    4038 
   Size:        153      32    3853

  Fragments arranged by size: 

                3853     153      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     862    2944    4038 
   Size:        862    2082    1094

  Fragments arranged by size: 

                2082    1094     862
 
XbaI T'CTAG_A

Cuts at:      0    2661    4038 
   Size:       2661    1377
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1113    3075    3513    3570    3999    4038 
   Size:       1113    1962     438      57     429      39

  Fragments arranged by size: 

                1962    1113     438     429      57      39
 
XmaI C'CCGG_G

Cuts at:      0    3455    4038 
   Size:       3455     583
 
ZraI GAC'GTC

Cuts at:      0     429    1677    4038 
   Size:        429    1248    2361

  Fragments arranged by size: 

                2361    1248     429
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI     AleI     AloI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BclI    BfrBI
     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NsiI     NspI     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI     PsrI     PstI    PvuII
     SapI     ScaI    SexAI     SfcI     SmaI     SmlI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AlfI    AlwNI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI    BciVI    BglII     BlpI    BmgBI     BmtI    BsaBI    BsiEI
    BsiWI     BsmI    BsmBI    BsrBI   BssHII    BstBI    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI     EagI  EcoICRI     FseI     FspI
    FspAI     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PciI     PmeI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SwaI     XhoI     XmnI
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