Restriction map for pF1KSDA0757
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvaII       AviII       AvrII       BbrPI       BcgI        
BclI        BfrI        BglII       BlnI        BsaBI       
BsaWI       BseAI       BsiWI       BsmI        BspEI       
BspLU11I    BsrDI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      Cfr10I      CpoI        DraI        DrdI        
Eam1105I    Eco81I      EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MslI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        976  GGTACC               G^GTACC
AccI          369  GTCGAC               GT^[AC][GT]AC
ApaI          345  GGGCCC               GGGCC^C
Asp700I       315  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       976  GGTACC               G^GTACC
AvaI          750  CTCGAG               C^[CT]CG[GA]G
BamHI         165  GGATCC               G^GATCC
BbsI          818  GTCTTC               GAAGAC(2/6)
BbvCI        1449  CCTCAGC              CCTCAGC(-5/-2)
BglI         1270  GCCCACGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         818  GTCTTC               GAAGAC(2/6)
BsaAI          55  TACGTG               [CT]AC^GT[GA]
BsmBI         277  GAGACG               CGTCTC(1/5)
BsmFI         851  GGGAC                GGGAC(10/14)
BsoBI         750  CTCGAG               C^[CT]CG[GA]G
BspDI         176  ATCGAT               AT^CGAT
BspHI        1257  TCATGA               T^CATGA
BspMI        1411  ACCTGC               ACCTGC(4/8)
BsrBI         706  GAGCGG               CCGCTC(-3/-3)
BstXI          11  CCAGTTCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          176  ATCGAT               AT^CGAT
EagI         1060  CGGCCG               C^GGCCG
EclXI        1060  CGGCCG               C^GGCCG
Eco52I       1060  CGGCCG               C^GGCCG
EcoNI         153  CCTGCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         319  GAATTC               G^AATTC
HincII        369  GTCGAC               GT[CT]^[GA]AC
HindII        369  GTCGAC               GT[CT]^[GA]AC
KpnI          976  GGTACC               GGTAC^C
NcoI          899  CCATGG               C^CATGG
NspI          269  GCATGC               [GA]CATG^[CT]
PaeR7I        750  CTCGAG               C^TCGAG
PstI         1167  CTGCAG               CTGCA^G
PvuII        1516  CAGCTG               CAG^CTG
RcaI         1257  TCATGA               T^CATGA
SalI          369  GTCGAC               G^TCGAC
SfcI         1167  CTGCAG               C^T[GA][CT]AG
SphI          269  GCATGC               GCATG^C
TfiI          179  GATTC                G^A[AT]TC
XcmI          671  CCATGTCACGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          750  CTCGAG               C^TCGAG
XmnI          315  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AcsI           28  AAATTC               [GA]^AATT[CT]
              319  GAATTC              
AlwNI         774  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1016  CAGACTCTG           
ApoI           28  AAATTC               [GA]^AATT[CT]
              319  GAATTC              
BalI          503  TGGCCA               TGG^CCA
              902  TGGCCA              
BbeI          259  GGCGCC               GGCGC^C
             1399  GGCGCC              
BciVI          21  GGATAC               GTATCC(6/5)
               81  GGATAC              
BfaI          239  CTAG                 C^TAG
              529  CTAG                
BlpI          147  GCTCAGC              GC^T[ACGT]AGC
              228  GCTCAGC             
Bpu1102I      147  GCTCAGC              GC^T[ACGT]AGC
              228  GCTCAGC             
BsaI          463  GAGACC               GGTCTC(1/5)
              483  GGTCTC              
BseRI         234  CTCCTC               GAGGAG(10/8)
             1173  CTCCTC              
BsiEI        1060  CGGCCG               CG[GA][CT]^CG
             1320  CGACCG              
Bsp1407I     1010  TGTACA               T^GTACA
             1553  TGTACA              
BsrGI        1010  TGTACA               T^GTACA
             1553  TGTACA              
BstYI         165  GGATCC               [GA]^GATC[CT]
             1005  GGATCT              
CelII         147  GCTCAGC              GC^T[ACGT]AGC
              228  GCTCAGC             
DraII         345  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1313  AGGGCCT             
DraIII        157  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1416  CACCCAGTG           
EarI          295  CTCTTC               CTCTTC(1/4)
              382  GAAGAG              
EcoO109I      345  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1313  AGGGCCT             
EcoT14I       716  CCAAGG               C^C[AT][AT]GG
              899  CCATGG              
HgaI          625  GACGC                GACGC(5/10)
             1441  GACGC               
HphI          474  GGTGA                GGTGA(8/7)
             1205  TCACC               
KasI          259  GGCGCC               G^GCGCC
             1399  GGCGCC              
Ksp632I       295  CTCTTC               CTCTTC(1/4)
              382  GAAGAG              
MaeI          239  CTAG                 C^TAG
              529  CTAG                
MaeII          56  ACGT                 A^CGT
             1001  ACGT                
MflI          165  GGATCC               [GA]^GATC[CT]
             1005  GGATCT              
MluNI         503  TGGCCA               TGG^CCA
              902  TGGCCA              
MscI          503  TGGCCA               TGG^CCA
              902  TGGCCA              
NarI          259  GGCGCC               GG^CGCC
             1399  GGCGCC              
SfoI          259  GGCGCC               GGC^GCC
             1399  GGCGCC              
SmlI          750  CTCGAG               C^T[CT][GA]AG
              859  CTCAAG              
SspBI        1010  TGTACA               T^GTACA
             1553  TGTACA              
StuI          806  AGGCCT               AGG^CCT
             1247  AGGCCT              
StyI          716  CCAAGG               C^C[AT][AT]GG
              899  CCATGG              
Tsp509I        29  AATT                 ^AATT
              320  AATT                
XhoII         165  GGATCC               [GA]^GATC[CT]
             1005  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        Aor51HI     AspHI       BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
Eco47III    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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