Restriction map for pF1KSDA0757
 (Linear) (Six-base) MAPSORT of: KIAA0757.seq  Check: 7168  from: 1  to: 1560



KIAA0757.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1420    1560 
   Size:       1420     140
 
AccI GT'mk_AC

Cuts at:      0     370    1560 
   Size:        370    1190
 
Acc65I G'GTAC_C

Cuts at:      0     976    1560 
   Size:        976     584
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     823    1560 
   Size:        249     574     737

  Fragments arranged by size: 

                 737     574     249
 
AfeI AGC'GCT

Cuts at:      0    1111    1189    1455    1560 
   Size:       1111      78     266     105

  Fragments arranged by size: 

                1111     266     105      78
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29    1513    1545    1560 
   Size:         29    1484      32      15

  Fragments arranged by size: 

                1484      32      29      15
 
AlwNI CAG_nnn'CTG

Cuts at:      0     779    1021    1560 
   Size:        779     242     539

  Fragments arranged by size: 

                 779     539     242
 
ApaI G_GGCC'C

Cuts at:      0     349    1560 
   Size:        349    1211
 
ApoI r'AATT_y

Cuts at:      0      28     319    1560 
   Size:         28     291    1241

  Fragments arranged by size: 

                1241     291      28
 
AvaI C'yCGr_G

Cuts at:      0     750    1560 
   Size:        750     810
 
BamHI G'GATC_C

Cuts at:      0     165    1560 
   Size:        165    1395
 
BanI G'GyrC_C

Cuts at:      0     259     976    1399    1560 
   Size:        259     717     423     161

  Fragments arranged by size: 

                 717     423     259     161
 
BanII G_rGCy'C

Cuts at:      0     217     252     349     458     497    1560 
   Size:        217      35      97     109      39    1063

  Fragments arranged by size: 

                1063     217     109      97      39      35
 
BbeI G_GCGC'C

Cuts at:      0     263    1403    1560 
   Size:        263    1140     157

  Fragments arranged by size: 

                1140     263     157
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     811    1560 
   Size:        811     749
 
BbvCI CC'TCA_GC

Cuts at:      0    1450    1560 
   Size:       1450     110
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      15      75    1560 
   Size:         15      60    1485

  Fragments arranged by size: 

                1485      60      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1276    1560 
   Size:       1276     284
 
BlpI GC'TnA_GC

Cuts at:      0     148     229    1560 
   Size:        148      81    1331

  Fragments arranged by size: 

                1331     148      81
 
Bme1580I G_kGCm'C

Cuts at:      0     349     436    1560 
   Size:        349      87    1124

  Fragments arranged by size: 

                1124     349      87
 
BmgBI CAC'GTC

Cuts at:      0    1002    1560 
   Size:       1002     558
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       5     789    1413    1560 
   Size:          5     784     624     147

  Fragments arranged by size: 

                 784     624     147       5
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     703    1560 
   Size:        703     857
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     454     673    1199    1560 
   Size:        295     159     219     526     361

  Fragments arranged by size: 

                 526     361     295     219     159
 
Bpu10I CC'TnA_GC

Cuts at:      0     403     425    1450    1560 
   Size:        403      22    1025     110

  Fragments arranged by size: 

                1025     403     110      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844    1560 
   Size:        844     716
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     457     489    1560 
   Size:        457      32    1071

  Fragments arranged by size: 

                1071     457      32
 
BsaAI yAC'GTr

Cuts at:      0      57    1560 
   Size:         57    1503
 
BsaHI Gr'CG_yC

Cuts at:      0     260     626    1400    1442    1560 
   Size:        260     366     774      42     118

  Fragments arranged by size: 

                 774     366     260     118      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1197    1227    1324    1354    1560 
   Size:       1197      30      97      30     206

  Fragments arranged by size: 

                1197     206      97      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     225    1164    1560 
   Size:        225     939     396

  Fragments arranged by size: 

                 939     396     225
 
BseYI C'CCAG_C

Cuts at:      0     429     949    1228    1240    1535    1560 
   Size:        429     520     279      12     295      25

  Fragments arranged by size: 

                 520     429     295     279      25      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     139     238     620    1398    1477    1560 
   Size:         79      60      99     382     778      79      83

  Fragments arranged by size: 

                 778     382      99      83      79      79      60
 
BsiEI CG_ry'CG

Cuts at:      0    1063    1323    1560 
   Size:       1063     260     237

  Fragments arranged by size: 

                1063     260     237
 
BsiHKAI G_wGCw'C

Cuts at:      0     217     458     497     659    1560 
   Size:        217     241      39     162     901

  Fragments arranged by size: 

                 901     241     217     162      39
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     271    1560 
   Size:        271    1289
 
Bsp1286I G_dGCh'C

Cuts at:      0     217     252     349     436     458     497     659    1560 
   Size:        217      35      97      87      22      39     162     901

  Fragments arranged by size: 

                 901     217     162      97      87      39      35      22
            
 
BspHI T'CATG_A

Cuts at:      0    1257    1560 
   Size:       1257     303
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1420    1560 
   Size:       1420     140
 
BsrBI CCG'CTC

Cuts at:      0     708    1560 
   Size:        708     852
 
BsrGI T'GTAC_A

Cuts at:      0    1010    1553    1560 
   Size:       1010     543       7

  Fragments arranged by size: 

                1010     543       7
 
BssSI C'ACGA_G

Cuts at:      0     457     677    1549    1560 
   Size:        457     220     872      11

  Fragments arranged by size: 

                 872     457     220      11
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      18    1560 
   Size:         18    1542
 
BstYI r'GATC_y

Cuts at:      0     165    1005    1560 
   Size:        165     840     555

  Fragments arranged by size: 

                 840     555     165
 
BtgI C'CryG_G

Cuts at:      0     307     899     915    1272    1560 
   Size:        307     592      16     357     288

  Fragments arranged by size: 

                 592     357     307     288      16
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     128    1560 
   Size:        128    1432
 
BtsI GCAGTG_nn'

Cuts at:      0     434     603    1560 
   Size:        434     169     957

  Fragments arranged by size: 

                 957     434     169
 
ClaI AT'CG_AT

Cuts at:      0     177    1560 
   Size:        177    1383
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     827     862    1560 
   Size:        827      35     698

  Fragments arranged by size: 

                 827     698      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     162    1421    1560 
   Size:        162    1259     139

  Fragments arranged by size: 

                1259     162     139
 
EaeI y'GGCC_r

Cuts at:      0     503     698     713     902    1060    1560 
   Size:        503     195      15     189     158     500

  Fragments arranged by size: 

                 503     500     195     189     158      15
 
EagI C'GGCC_G

Cuts at:      0    1060    1560 
   Size:       1060     500
 
EarI CTCTTCn'nnn_

Cuts at:      0     301     377    1560 
   Size:        301      76    1183

  Fragments arranged by size: 

                1183     301      76
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     865    1122    1560 
   Size:        865     257     438

  Fragments arranged by size: 

                 865     438     257
 
EcoICRI GAG'CTC

Cuts at:      0     215     456     495    1560 
   Size:        215     241      39    1065

  Fragments arranged by size: 

                1065     241     215      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     295     454     673     823    1199    1560 
   Size:        249      46     159     219     150     376     361

  Fragments arranged by size: 

                 376     361     249     219     159     150      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     157    1560 
   Size:        157    1403
 
EcoO109I rG'GnC_Cy

Cuts at:      0     346    1314    1560 
   Size:        346     968     246

  Fragments arranged by size: 

                 968     346     246
 
EcoRI G'AATT_C

Cuts at:      0     319    1560 
   Size:        319    1241
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      57    1560 
   Size:         57    1503
 
HaeII r_GCGC'y

Cuts at:      0     263    1113    1191    1235    1403    1457    1560 
   Size:        263     850      78      44     168      54     103

  Fragments arranged by size: 

                 850     263     168     103      78      54      44
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1078    1110    1560 
   Size:       1078      32     450

  Fragments arranged by size: 

                1078     450      32
 
HincII GTy'rAC

Cuts at:      0     371    1560 
   Size:        371    1189
 
KasI G'GCGC_C

Cuts at:      0     259    1399    1560 
   Size:        259    1140     161

  Fragments arranged by size: 

                1140     259     161
 
KpnI G_GTAC'C

Cuts at:      0     980    1560 
   Size:        980     580
 
MscI TGG'CCA

Cuts at:      0     505     904    1560 
   Size:        505     399     656

  Fragments arranged by size: 

                 656     505     399
 
MspA1I CmG'CkG

Cuts at:      0     102    1376    1518    1560 
   Size:        102    1274     142      42

  Fragments arranged by size: 

                1274     142     102      42
 
NarI GG'CG_CC

Cuts at:      0     260    1400    1560 
   Size:        260    1140     160

  Fragments arranged by size: 

                1140     260     160
 
NcoI C'CATG_G

Cuts at:      0     899    1560 
   Size:        899     661
 
NspI r_CATG'y

Cuts at:      0     273    1560 
   Size:        273    1287
 
PasI CC'CwG_GG

Cuts at:      0    1028    1560 
   Size:       1028     532
 
PspOMI G'GGCC_C

Cuts at:      0     345    1560 
   Size:        345    1215
 
PspXI vC'TCGA_Gb

Cuts at:      0     750    1560 
   Size:        750     810
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      46      78    1560 
   Size:         46      32    1482

  Fragments arranged by size: 

                1482      46      32
 
PstI C_TGCA'G

Cuts at:      0    1171    1560 
   Size:       1171     389
 
PvuII CAG'CTG

Cuts at:      0    1518    1560 
   Size:       1518      42
 
SacI G_AGCT'C

Cuts at:      0     217     458     497    1560 
   Size:        217     241      39    1063

  Fragments arranged by size: 

                1063     241     217      39
 
SalI G'TCGA_C

Cuts at:      0     369    1560 
   Size:        369    1191
 
SfcI C'TryA_G

Cuts at:      0    1167    1560 
   Size:       1167     393
 
SfoI GGC'GCC

Cuts at:      0     261    1401    1560 
   Size:        261    1140     159

  Fragments arranged by size: 

                1140     261     159
 
SmlI C'TyrA_G

Cuts at:      0     750     859    1560 
   Size:        750     109     701

  Fragments arranged by size: 

                 750     701     109
 
SphI G_CATG'C

Cuts at:      0     273    1560 
   Size:        273    1287
 
StuI AGG'CCT

Cuts at:      0     808    1249    1560 
   Size:        808     441     311

  Fragments arranged by size: 

                 808     441     311
 
StyI C'CwwG_G

Cuts at:      0     716     899    1560 
   Size:        716     183     661

  Fragments arranged by size: 

                 716     661     183
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1432    1549    1560 
   Size:       1432     117      11

  Fragments arranged by size: 

                1432     117      11
 
TatI w'GTAC_w

Cuts at:      0    1010    1553    1560 
   Size:       1010     543       7

  Fragments arranged by size: 

                1010     543       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1197    1229    1324    1330    1356    1362    1560 
   Size:       1197      32      95       6      26       6     198

  Fragments arranged by size: 

                1197     198      95      32      26       6       6
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     678    1560 
   Size:        678     882
 
XhoI C'TCGA_G

Cuts at:      0     750    1560 
   Size:        750     810
 
XmnI GAAnn'nnTTC

Cuts at:      0     319    1560 
   Size:        319    1241
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AloI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrGI    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     KasI
     KpnI     MscI   MspA1I     NarI     NcoI     NspI     PasI   PspOMI
    PspXI     PsrI     PstI    PvuII     SacI     SalI     SfcI     SfoI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AleI     AlfI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI     BclI
    BfrBI    BglII     BmtI    BsaBI    BsaWI    BsiWI     BsmI    BspEI
    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     DraI     DrdI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     MfeI     MluI     MmeI     MslI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI     PvuI    RsrII    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI
     XmaI     ZraI
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