Restriction map for pF1KSDA0741
 (Linear) (Six-base) MAPSORT of: KIAA0741.seq  Check: 5885  from: 1  to: 3660



KIAA0741.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1972    3660 
   Size:       1972    1688
 
Acc65I G'GTAC_C

Cuts at:      0    2460    3660 
   Size:       2460    1200
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     905    1613    3047    3658    3660 
   Size:        905     708    1434     611       2

  Fragments arranged by size: 

                1434     905     708     611       2
 
AflIII A'CryG_T

Cuts at:      0    1962    3660 
   Size:       1962    1698
 
AleI CACnn'nnGTG

Cuts at:      0    2449    3372    3660 
   Size:       2449     923     288

  Fragments arranged by size: 

                2449     923     288
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1550    1582    3660 
   Size:       1550      32    2078

  Fragments arranged by size: 

                2078    1550      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1771    3660 
   Size:       1771    1889
 
ApaI G_GGCC'C

Cuts at:      0    2687    3660 
   Size:       2687     973
 
ApoI r'AATT_y

Cuts at:      0     268     540     574     823    1937    2058    2356    2389 
   Size:        268     272      34     249    1114     121     298      33

Cuts at:   2389    2986    3164    3247    3390    3660 
   Size:        597     178      83     143     270

  Fragments arranged by size: 

                1114     597     298     272     270     268     249     178
                 143     121      83      34      33
 
AseI AT'TA_AT

Cuts at:      0    2033    3660 
   Size:       2033    1627
 
AvaI C'yCGr_G

Cuts at:      0    2598    3311    3660 
   Size:       2598     713     349

  Fragments arranged by size: 

                2598     713     349
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     854     887    3660 
   Size:        854      33    2773

  Fragments arranged by size: 

                2773     854      33
 
BanI G'GyrC_C

Cuts at:      0    2460    2608    3660 
   Size:       2460     148    1052

  Fragments arranged by size: 

                2460    1052     148
 
BanII G_rGCy'C

Cuts at:      0    1765    1893    2204    2687    3660 
   Size:       1765     128     311     483     973

  Fragments arranged by size: 

                1765     973     483     311     128
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      35    1735    1936    3525    3660 
   Size:         35    1700     201    1589     135

  Fragments arranged by size: 

                1700    1589     201     135      35
 
BclI T'GATC_A

Cuts at:      0    2630    3660 
   Size:       2630    1030
 
BfrBI ATG'CAT

Cuts at:      0    2190    3660 
   Size:       2190    1470
 
BlpI GC'TnA_GC

Cuts at:      0    1046    3660 
   Size:       1046    2614
 
Bme1580I G_kGCm'C

Cuts at:      0    2611    2687    3011    3660 
   Size:       2611      76     324     649

  Fragments arranged by size: 

                2611     649     324      76
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2971    3028    3660 
   Size:       2971      57     632

  Fragments arranged by size: 

                2971     632      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     887    2693    2833    3660 
   Size:        887    1806     140     827

  Fragments arranged by size: 

                1806     887     827     140
 
Bpu10I CC'TnA_GC

Cuts at:      0    1283    2783    3660 
   Size:       1283    1500     877

  Fragments arranged by size: 

                1500    1283     877
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    3660 
   Size:        191    3469
 
BsaBI GATnn'nnATC

Cuts at:      0    1790    3283    3660 
   Size:       1790    1493     377

  Fragments arranged by size: 

                1790    1493     377
 
BsaHI Gr'CG_yC

Cuts at:      0     659    3660 
   Size:        659    3001
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1446    1476    2930    2960    3660 
   Size:       1446      30    1454      30     700

  Fragments arranged by size: 

                1454    1446     700      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     508    1552    1612    1615    1618    1621    1663    1672 
   Size:        508    1044      60       3       3       3      42       9

Cuts at:   1672    2377    2711    3660 
   Size:        705     334     949

  Fragments arranged by size: 

                1044     949     705     508     334      60      42       9
                   3       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2456    3194    3660 
   Size:       2456     738     466

  Fragments arranged by size: 

                2456     738     466
 
BsiHKAI G_wGCw'C

Cuts at:      0    1765    2574    3660 
   Size:       1765     809    1086

  Fragments arranged by size: 

                1765    1086     809
 
BsmI GAATG_Cn'

Cuts at:      0    2096    2415    3660 
   Size:       2096     319    1245

  Fragments arranged by size: 

                2096    1245     319
 
Bsp1286I G_dGCh'C

Cuts at:      0    1765    1893    2204    2574    2611    2687    3011    3660 
   Size:       1765     128     311     370      37      76     324     649

  Fragments arranged by size: 

                1765     649     370     324     311     128      76      37
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1972    3337    3660 
   Size:       1972    1365     323

  Fragments arranged by size: 

                1972    1365     323
 
BsrDI GCAATG_nn'

Cuts at:      0    2162    3660 
   Size:       2162    1498
 
BsrFI r'CCGG_y

Cuts at:      0    3560    3660 
   Size:       3560     100
 
BsrGI T'GTAC_A

Cuts at:      0    1965    3660 
   Size:       1965    1695
 
BstYI r'GATC_y

Cuts at:      0    2436    3315    3660 
   Size:       2436     879     345

  Fragments arranged by size: 

                2436     879     345
 
BtsI GCAGTG_nn'

Cuts at:      0     245    1683    3660 
   Size:        245    1438    1977

  Fragments arranged by size: 

                1977    1438     245
 
DraI TTT'AAA

Cuts at:      0    2327    3660 
   Size:       2327    1333
 
DraIII CAC_nnn'GTG

Cuts at:      0    2557    3660 
   Size:       2557    1103
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2214    3660 
   Size:       2214    1446
 
EaeI y'GGCC_r

Cuts at:      0    1526    3660 
   Size:       1526    2134
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     764     881    1069    1076    1127    1318    1445 
   Size:        257     507     117     188       7      51     191     127

Cuts at:   1445    1589    1610    1640    1649    2555    3604    3660 
   Size:        144      21      30       9     906    1049      56

  Fragments arranged by size: 

                1049     906     507     257     191     188     144     127
                 117      56      51      30      21       9       7
 
EcoICRI GAG'CTC

Cuts at:      0    1763    3660 
   Size:       1763    1897
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     887     905    1613    2693    2833    3047    3658    3660 
   Size:        887      18     708    1080     140     214     611       2

  Fragments arranged by size: 

                1080     887     708     611     214     140      18       2
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     625    1003    2683    3660 
   Size:        625     378    1680     977

  Fragments arranged by size: 

                1680     977     625     378
 
EcoRI G'AATT_C

Cuts at:      0     268    3660 
   Size:        268    3392
 
EcoRV GAT'ATC

Cuts at:      0     171    3660 
   Size:        171    3489
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     171    1234    1282    3660 
   Size:        171    1063      48    2378

  Fragments arranged by size: 

                2378    1063     171      48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     349     381     646     678    1446    1478    2645    2677 
   Size:        349      32     265      32     768      32    1167      32

Cuts at:   2677    2930    2962    3660 
   Size:        253      32     698

  Fragments arranged by size: 

                1167     768     698     349     265     253      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2522    3399    3660 
   Size:       2522     877     261

  Fragments arranged by size: 

                2522     877     261
 
HindIII A'AGCT_T

Cuts at:      0    1048    3660 
   Size:       1048    2612
 
HpaI GTT'AAC

Cuts at:      0    2522    3660 
   Size:       2522    1138
 
KpnI G_GTAC'C

Cuts at:      0    2464    3660 
   Size:       2464    1196
 
MfeI C'AATT_G

Cuts at:      0    2210    3660 
   Size:       2210    1450
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3053    3660 
   Size:       3053     607
 
MscI TGG'CCA

Cuts at:      0    1528    3660 
   Size:       1528    2132
 
MslI CAynn'nnrTG

Cuts at:      0    1961    2449    2480    3372    3660 
   Size:       1961     488      31     892     288

  Fragments arranged by size: 

                1961     892     488     288      31
 
MspA1I CmG'CkG

Cuts at:      0     910    3660 
   Size:        910    2750
 
NaeI GCC'GGC

Cuts at:      0    3562    3660 
   Size:       3562      98
 
NgoMIV G'CCGG_C

Cuts at:      0    3560    3660 
   Size:       3560     100
 
NsiI A_TGCA'T

Cuts at:      0    2192    3660 
   Size:       2192    1468
 
NspI r_CATG'y

Cuts at:      0    1918    1966    3660 
   Size:       1918      48    1694

  Fragments arranged by size: 

                1918    1694      48
 
PciI A'CATG_T

Cuts at:      0    1962    3660 
   Size:       1962    1698
 
PfoI T'CCnGG_A

Cuts at:      0    2085    2670    3660 
   Size:       2085     585     990

  Fragments arranged by size: 

                2085     990     585
 
PpuMI rG'GwC_Cy

Cuts at:      0     625    1003    3660 
   Size:        625     378    2657

  Fragments arranged by size: 

                2657     625     378
 
PsiI TTA'TAA

Cuts at:      0    2852    3660 
   Size:       2852     808
 
PspOMI G'GGCC_C

Cuts at:      0    2683    3660 
   Size:       2683     977
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1421    1453    3660 
   Size:       1421      32    2207

  Fragments arranged by size: 

                2207    1421      32
 
PstI C_TGCA'G

Cuts at:      0      72     915    3660 
   Size:         72     843    2745

  Fragments arranged by size: 

                2745     843      72
 
PvuII CAG'CTG

Cuts at:      0     910    3660 
   Size:        910    2750
 
SacI G_AGCT'C

Cuts at:      0    1765    3660 
   Size:       1765    1895
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2555    3660 
   Size:       2555    1105
 
SfcI C'TryA_G

Cuts at:      0      68     911    1415    2615    3533    3660 
   Size:         68     843     504    1200     918     127

  Fragments arranged by size: 

                1200     918     843     504     127      68
 
SmaI CCC'GGG

Cuts at:      0    2600    3660 
   Size:       2600    1060
 
SmlI C'TyrA_G

Cuts at:      0     206    3311    3660 
   Size:        206    3105     349

  Fragments arranged by size: 

                3105     349     206
 
StuI AGG'CCT

Cuts at:      0    2708    3660 
   Size:       2708     952
 
StyI C'CwwG_G

Cuts at:      0      38      62    3660 
   Size:         38      24    3598

  Fragments arranged by size: 

                3598      38      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3384    3519    3660 
   Size:       3384     135     141

  Fragments arranged by size: 

                3384     141     135
 
TatI w'GTAC_w

Cuts at:      0     398    1965    3296    3660 
   Size:        398    1567    1331     364

  Fragments arranged by size: 

                1567    1331     398     364
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2001    2033    3660 
   Size:       2001      32    1627

  Fragments arranged by size: 

                2001    1627      32
 
XbaI T'CTAG_A

Cuts at:      0     586    1941    3660 
   Size:        586    1355    1719

  Fragments arranged by size: 

                1719    1355     586
 
XhoI C'TCGA_G

Cuts at:      0    3311    3660 
   Size:       3311     349
 
XmaI C'CCGG_G

Cuts at:      0    2598    3660 
   Size:       2598    1062
 
XmnI GAAnn'nnTTC

Cuts at:      0     540    3660 
   Size:        540    3120
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AloI    AlwNI     ApaI
     ApoI     AseI     AvaI     BaeI     BanI    BanII     BbsI     BclI
    BfrBI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI    BstYI     BtsI     DraI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PciI     PfoI
    PpuMI     PsiI   PspOMI     PsrI     PstI    PvuII     SacI     SapI
     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AlfI
    ApaLI     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI     BcgI
    BciVI     BglI    BglII    BmgBI     BmtI     BplI     BsaI    BsaAI
    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     EagI     EciI    EcoNI     FseI
     FspI    FspAI    HaeII     KasI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI    PshAI    PspXI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XcmI     ZraI
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