Restriction map for pF1KSDA0724
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AccIII      AclI        AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
AviII       BamHI       BbeI        BbrPI       BsaWI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BsrDI       BssHII      BssSI       BstBI       
BstEII      BstPI       ClaI        CpoI        DraI        
DraIII      EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoT22I     FseI        FspI        
HindIII     HpaI        KasI        KspI        MluI        
MroI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII        1441  GACGTC               GACGT^C
Acc65I        210  GGTACC               G^GTACC
AccII        2794  CGCG                 CG^CG
AcsI         1262  GAATTT               [GA]^AATT[CT]
AcyI         1441  GACGTC               G[GA]^CG[CT]C
AflII        1819  CTTAAG               C^TTAAG
AhdI         2243  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         2344  GGGCCC               GGGCC^C
ApoI         1262  GAATTT               [GA]^AATT[CT]
Asp700I      2832  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       210  GGTACC               G^GTACC
AspEI        2243  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1519  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          959  CCCGGG               C^[CT]CG[GA]G
AvrII        1611  CCTAGG               C^CTAGG
BcgI         2784  GCAGATCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2769  GGATAC               GTATCC(6/5)
BfrI         1819  CTTAAG               C^TTAAG
BglI          382  GCCTCACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1611  CCTAGG               C^CTAGG
BsaAI         823  TACGTG               [CT]AC^GT[GA]
BsaBI        1491  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1441  GACGTC               G[GA]^CG[CT]C
BsiEI        2583  CGGTCG               CG[GA][CT]^CG
BsmI          792  GAATGC               GAATGC(1/-1)
BsoBI         959  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     2201  TGTACA               T^GTACA
BsrBI           4  GAGCGG               CCGCTC(-3/-3)
BsrFI        1157  ACCGGC               [GA]^CCGG[CT]
BsrGI        2201  TGTACA               T^GTACA
Bst1107I     1141  GTATAC               GTA^TAC
BstUI        2794  CGCG                 CG^CG
BstZ17I      1141  GTATAC               GTA^TAC
Cfr10I       1157  ACCGGC               [GA]^CCGG[CT]
DrdI         1564  GACCACCCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2243  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2388  CCTGGCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2659  GATATC               GAT^ATC
Hin1I        1441  GACGTC               G[GA]^CG[CT]C
KpnI          210  GGTACC               GGTAC^C
MamI         1491  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2288  CAATTG               C^AATTG
MunI         2288  CAATTG               C^AATTG
MvnI         2794  CGCG                 CG^CG
NruI         2793  TCGCGA               TCG^CGA
PflFI        1519  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2016  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1954  GACATCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI          224  AGTACT               AGT^ACT
SexAI        2730  ACCTGGT              A^CC[AT]GGT
SmaI          959  CCCGGG               CCC^GGG
SphI         1605  GCATGC               GCATG^C
SspBI        2201  TGTACA               T^GTACA
Tth111I      1519  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         959  CCCGGG               C^CCGGG
XmaI          959  CCCGGG               C^CCGGG
XmnI         2832  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1141  GTATAC               GT^[AC][GT]AC
             1165  GTCTAC              
AocI          520  CCTGAGG              CC^T[ACGT]AGG
             2485  CCTGAGG             
BbvCI        2751  CCTCAGC              CCTCAGC(-5/-2)
              454  GCTGAGG             
BclI         1577  TGATCA               T^GATCA
             1974  TGATCA              
BfaI          490  CTAG                 C^TAG
             1612  CTAG                
BglII         254  AGATCT               A^GATCT
             1658  AGATCT              
BlpI         1326  GCTCAGC              GC^T[ACGT]AGC
             2073  GCTGAGC             
Bpu1102I     1326  GCTCAGC              GC^T[ACGT]AGC
             2073  GCTGAGC             
BstAPI        189  GCAGCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              882  GCAGGCAGTGC         
Bsu36I        520  CCTGAGG              CC^T[ACGT]AGG
             2485  CCTGAGG             
CelII        1326  GCTCAGC              GC^T[ACGT]AGC
             2073  GCTGAGC             
Eco81I        520  CCTGAGG              CC^T[ACGT]AGG
             2485  CCTGAGG             
FbaI         1577  TGATCA               T^GATCA
             1974  TGATCA              
HgaI         2142  GACGC                GACGC(5/10)
              654  GCGTC               
HincII       1009  GTCAAC               GT[CT]^[GA]AC
             1444  GTCAAC              
HindII       1009  GTCAAC               GT[CT]^[GA]AC
             1444  GTCAAC              
MaeI          490  CTAG                 C^TAG
             1612  CTAG                
MseI         1820  TTAA                 T^TAA
             2138  TTAA                
SacI          760  GAGCTC               GAGCT^C
             1541  GAGCTC              
TaqI          767  TCGA                 T^CGA
             2241  TCGA                
TfiI         1229  GATTC                G^A[AT]TC
             2371  GATTC               
Tru9I        1820  TTAA                 T^TAA
             2138  TTAA                
Tsp509I      1263  AATT                 ^AATT
             2289  AATT                
TthHB8I       767  TCGA                 T^CGA
             2241  TCGA                
XcmI          575  CCAGCCTGGCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2021  CCAACCCCGTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        PvuII       RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TseI        Tsp45I      
TspRI       Van91I      XhoII       
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