Restriction map for pF1KSDA0723
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        Asp718I     AspEI       
AvaI        AviII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfrI        BglI        BsaAI       BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FauI        FseI        FspI        
HgaI        Hin1I       HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1041  GTCTAC               GT^[AC][GT]AC
AflIII       1485  ACGTGT               A^C[GA][CT]GT
Alw44I        212  GTGCAC               G^TGCAC
AlwNI        2228  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         212  GTGCAC               G^TGCAC
AseI         1300  ATTAAT               AT^TAAT
AsnI         1300  ATTAAT               AT^TAAT
AspI          761  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         520  CCTAGG               C^CTAGG
BalI          335  TGGCCA               TGG^CCA
BanII         203  GAGCTC               G[GA]GC[CT]^C
BlnI          520  CCTAGG               C^CTAGG
BlpI         1549  GCTGAGC              GC^T[ACGT]AGC
BpmI         1793  CTCCAG               CTGGAG(16/14)
Bpu1102I     1549  GCTGAGC              GC^T[ACGT]AGC
BsaWI        1891  TCCGGT               [AT]^CCGG[AT]
BsgI          146  CTGCAC               GTGCAG(16/14)
BsmBI        2027  GAGACG               CGTCTC(1/5)
BsmI         1512  GCATTC               GAATGC(1/-1)
BspMI        1145  ACCTGC               ACCTGC(4/8)
BstAPI        148  GCACGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1549  GCTGAGC              GC^T[ACGT]AGC
CfoI          812  GCGC                 GCG^C
EaeI          335  TGGCCA               [CT]^GGCC[GA]
EcoNI        2389  CCTAAAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1061  GAATTC               G^AATTC
HaeII         811  GGCGCT               [GA]GCGC^[CT]
HapII        1892  CCGG                 C^CGG
HhaI          812  GCGC                 GCG^C
HinP1I        812  GCGC                 G^CGC
HpaII        1892  CCGG                 C^CGG
MluNI         335  TGGCCA               TGG^CCA
MscI          335  TGGCCA               TGG^CCA
MspA1I       1249  CAGCTG               C[AC]G^C[GT]G
MspI         1892  CCGG                 C^CGG
NspI         1163  ACATGC               [GA]CATG^[CT]
PflFI         761  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1102  CCAGCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1300  ATTAAT               AT^TAAT
PvuII        1249  CAGCTG               CAG^CTG
SacI          203  GAGCTC               GAGCT^C
ScaI          229  AGTACT               AGT^ACT
SexAI        2226  ACCAGGT              A^CC[AT]GGT
SfcI          427  CTACAG               C^T[GA][CT]AG
SspI          829  AATATT               AAT^ATT
StuI           78  AGGCCT               AGG^CCT
Tth111I       761  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1102  CCAGCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          323  CCAGTAACATTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwI         1021  GATCC                GGATC(4/5)
             2257  GATCC               
BclI         1464  TGATCA               T^GATCA
             2169  TGATCA              
BsaI          712  GAGACC               GGTCTC(1/5)
             2032  GAGACC              
BseRI         815  CTCCTC               GAGGAG(10/8)
              441  GAGGAG              
EarI          554  GAAGAG               CTCTTC(1/4)
             1882  GAAGAG              
FbaI         1464  TGATCA               T^GATCA
             2169  TGATCA              
HindIII      1543  AAGCTT               A^AGCTT
             2137  AAGCTT              
Ksp632I       554  GAAGAG               CTCTTC(1/4)
             1882  GAAGAG              
MaeII         762  ACGT                 A^CGT
             1485  ACGT                
NcoI          609  CCATGG               C^CATGG
              843  CCATGG              
PpuMI        1068  GGGACCT              [GA]G^G[AT]CC[CT]
             1152  AGGACCT             
TaqI          951  TCGA                 T^CGA
             2159  TCGA                
Tsp45I         56  GTGAC                ^GT[GC]AC
              941  GTCAC               
TthHB8I       951  TCGA                 T^CGA
             2159  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     AspHI       AvaII       BbsI        BbvI        
BfaI        BglII       BmyI        BpuAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HincII      
HindII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com