Restriction map for pF1KSDA0723
 (Linear) (Six-base) MAPSORT of: KIAA0723.seq  Check: 2191  from: 1  to: 2541



KIAA0723.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AccI GT'mk_AC

Cuts at:      0    1042    2541 
   Size:       1042    1499
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470    1460    1982    2102    2541 
   Size:        470     990     522     120     439

  Fragments arranged by size: 

                 990     522     470     439     120
 
AflIII A'CryG_T

Cuts at:      0    1485    2541 
   Size:       1485    1056
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2233    2541 
   Size:       2233     308
 
ApaLI G'TGCA_C

Cuts at:      0     212    2541 
   Size:        212    2329
 
ApoI r'AATT_y

Cuts at:      0     408     733    1061    2494    2541 
   Size:        408     325     328    1433      47

  Fragments arranged by size: 

                1433     408     328     325      47
 
AseI AT'TA_AT

Cuts at:      0    1301    2541 
   Size:       1301    1240
 
AvrII C'CTAG_G

Cuts at:      0     520    2541 
   Size:        520    2021
 
BanII G_rGCy'C

Cuts at:      0     207    2541 
   Size:        207    2334
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     212     842    1855    2541 
   Size:        212     630    1013     686

  Fragments arranged by size: 

                1013     686     630     212
 
BclI T'GATC_A

Cuts at:      0    1464    2169    2541 
   Size:       1464     705     372

  Fragments arranged by size: 

                1464     705     372
 
BglII A'GATC_T

Cuts at:      0     487     787    1783    2541 
   Size:        487     300     996     758

  Fragments arranged by size: 

                 996     758     487     300
 
BlpI GC'TnA_GC

Cuts at:      0    1550    2541 
   Size:       1550     991
 
Bme1580I G_kGCm'C

Cuts at:      0     216    2541 
   Size:        216    2325
 
BmgBI CAC'GTC

Cuts at:      0     763    1486    2541 
   Size:        763     723    1055

  Fragments arranged by size: 

                1055     763     723
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      93     586    2541 
   Size:         93     493    1955

  Fragments arranged by size: 

                1955     493      93
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     298     830    2541 
   Size:        298     532    1711

  Fragments arranged by size: 

                1711     532     298
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1778    2541 
   Size:       1778     763
 
Bpu10I CC'TnA_GC

Cuts at:      0    1340    2541 
   Size:       1340    1201
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     486     608    1251    1525    2541 
   Size:        486     122     643     274    1016

  Fragments arranged by size: 

                1016     643     486     274     122
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     706    2026    2541 
   Size:        706    1320     515

  Fragments arranged by size: 

                1320     706     515
 
BsaWI w'CCGG_w

Cuts at:      0    1891    2541 
   Size:       1891     650
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     456     806    2541 
   Size:        456     350    1735

  Fragments arranged by size: 

                1735     456     350
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131    2541 
   Size:        131    2410
 
BsiHKAI G_wGCw'C

Cuts at:      0     207     216     880    2541 
   Size:        207       9     664    1661

  Fragments arranged by size: 

                1661     664     207       9
 
BsmI GAATG_Cn'

Cuts at:      0    1512    2541 
   Size:       1512    1029
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2021    2541 
   Size:       2021     520
 
Bsp1286I G_dGCh'C

Cuts at:      0     207     216     880    2541 
   Size:        207       9     664    1661

  Fragments arranged by size: 

                1661     664     207       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1154    2541 
   Size:       1154    1387
 
BsrDI GCAATG_nn'

Cuts at:      0    1640    1646    2457    2541 
   Size:       1640       6     811      84

  Fragments arranged by size: 

                1640     811      84       6
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     154    2541 
   Size:        154    2387
 
BstYI r'GATC_y

Cuts at:      0     487     787    1783    2541 
   Size:        487     300     996     758

  Fragments arranged by size: 

                 996     758     487     300
 
BtgI C'CryG_G

Cuts at:      0     303     609     843    2541 
   Size:        303     306     234    1698

  Fragments arranged by size: 

                1698     306     303     234
 
BtsI GCAGTG_nn'

Cuts at:      0    1355    1999    2541 
   Size:       1355     644     542

  Fragments arranged by size: 

                1355     644     542
 
EaeI y'GGCC_r

Cuts at:      0     335    2541 
   Size:        335    2206
 
EarI CTCTTCn'nnn_

Cuts at:      0     549    1877    2541 
   Size:        549    1328     664

  Fragments arranged by size: 

                1328     664     549
 
EcoICRI GAG'CTC

Cuts at:      0     205    2541 
   Size:        205    2336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470    1460    1778    1982    2102    2541 
   Size:        470     990     318     204     120     439

  Fragments arranged by size: 

                 990     470     439     318     204     120
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2393    2541 
   Size:       2393     148
 
EcoO109I rG'GnC_Cy

Cuts at:      0     457    1069    1153    2541 
   Size:        457     612      84    1388

  Fragments arranged by size: 

                1388     612     457      84
 
EcoRI G'AATT_C

Cuts at:      0    1061    2541 
   Size:       1061    1480
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1619    2541 
   Size:       1619     922
 
HaeII r_GCGC'y

Cuts at:      0     815    2541 
   Size:        815    1726
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     366     398     472     504    1581    1613    1810    1842 
   Size:        366      32      74      32    1077      32     197      32

Cuts at:   1842    2541 
   Size:        699

  Fragments arranged by size: 

                1077     699     366     197      74      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     922    1647    1698    2541 
   Size:        922     725      51     843

  Fragments arranged by size: 

                 922     843     725      51
 
HindIII A'AGCT_T

Cuts at:      0    1543    2137    2541 
   Size:       1543     594     404

  Fragments arranged by size: 

                1543     594     404
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1089    2171    2541 
   Size:       1089    1082     370

  Fragments arranged by size: 

                1089    1082     370
 
MscI TGG'CCA

Cuts at:      0     337    2541 
   Size:        337    2204
 
MslI CAynn'nnrTG

Cuts at:      0      53     929    1582    2541 
   Size:         53     876     653     959

  Fragments arranged by size: 

                 959     876     653      53
 
MspA1I CmG'CkG

Cuts at:      0    1251    2541 
   Size:       1251    1290
 
NcoI C'CATG_G

Cuts at:      0     609     843    2541 
   Size:        609     234    1698

  Fragments arranged by size: 

                1698     609     234
 
NspI r_CATG'y

Cuts at:      0    1167    2541 
   Size:       1167    1374
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1108    2541 
   Size:       1108    1433
 
PpuMI rG'GwC_Cy

Cuts at:      0    1069    1153    2541 
   Size:       1069      84    1388

  Fragments arranged by size: 

                1388    1069      84
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2400    2432    2541 
   Size:       2400      32     109

  Fragments arranged by size: 

                2400     109      32
 
PvuII CAG'CTG

Cuts at:      0    1251    2541 
   Size:       1251    1290
 
SacI G_AGCT'C

Cuts at:      0     207    2541 
   Size:        207    2334
 
ScaI AGT'ACT

Cuts at:      0     231    2541 
   Size:        231    2310
 
SexAI A'CCwGG_T

Cuts at:      0    2226    2541 
   Size:       2226     315
 
SfcI C'TryA_G

Cuts at:      0     427    2541 
   Size:        427    2114
 
SmlI C'TyrA_G

Cuts at:      0     465     623    1230    1540    2541 
   Size:        465     158     607     310    1001

  Fragments arranged by size: 

                1001     607     465     310     158
 
SspI AAT'ATT

Cuts at:      0     831    2541 
   Size:        831    1710
 
StuI AGG'CCT

Cuts at:      0      80    2541 
   Size:         80    2461
 
StyI C'CwwG_G

Cuts at:      0     520     609     843    2541 
   Size:        520      89     234    1698

  Fragments arranged by size: 

                1698     520     234      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1006    1117    2050    2541 
   Size:       1006     111     933     491

  Fragments arranged by size: 

                1006     933     491     111
 
TatI w'GTAC_w

Cuts at:      0     229    2541 
   Size:        229    2312
 
Tth111I GACn'n_nGTC

Cuts at:      0     764    2541 
   Size:        764    1777
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     330    2541 
   Size:        330    2211
 
XmnI GAAnn'nnTTC

Cuts at:      0     180     618    2115    2541 
   Size:        180     438    1497     426

  Fragments arranged by size: 

                1497     438     426     180
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI    ApaLI     ApoI     AseI    AvrII
    BanII     BbsI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI   BstAPI    BstYI
     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NcoI     NspI    PflMI    PpuMI     PsrI    PvuII
     SacI     ScaI    SexAI     SfcI     SmlI     SspI     StuI     StyI
    TaqII     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI    AsiSI     AvaI     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BmtI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI    BsiWI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoRV     FseI     FspI    FspAI
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SwaI     TstI     XbaI     XhoI     XmaI     ZraI
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