Restriction map for pF1KSDA0722
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflIII      
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AvrII       BamHI       BbrPI       BciVI       BclI        
BglII       BlnI        BsaAI       BsaBI       BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        Eam1105I    
EcoRI       EcoRV       EcoT22I     FbaI        HincII      
HindII      HindIII     HpaI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspI        StuI        SwaI        TfiI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        509  GGTACC               G^GTACC
AflII        2107  CTTAAG               C^TTAAG
Aor51HI      2873  AGCGCT               AGC^GCT
Asp718I       509  GGTACC               G^GTACC
AspI         1066  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1469  TGGCCA               TGG^CCA
BfaI         1936  CTAG                 C^TAG
BfrI         2107  CTTAAG               C^TTAAG
BsaWI        1973  ACCGGA               [AT]^CCGG[AT]
BsmI         2909  GCATTC               GAATGC(1/-1)
BsrDI        2442  CATTGC               GCAATG(2/0)
BstEII       1096  GGTGACC              G^GT[ACGT]ACC
BstPI        1096  GGTGACC              G^GT[ACGT]ACC
Eco47III     2873  AGCGCT               AGC^GCT
EcoO65I      1096  GGTGACC              G^GT[ACGT]ACC
FseI         1495  GGCCGGCC             GGCCGG^CC
KpnI          509  GGTACC               GGTAC^C
KspI           12  CCGCGG               CCGC^GG
MaeI         1936  CTAG                 C^TAG
MluNI        1469  TGGCCA               TGG^CCA
MscI         1469  TGGCCA               TGG^CCA
NspI         3056  ACATGC               [GA]CATG^[CT]
PflFI        1066  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         243  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         711  GACGTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII          12  CCGCGG               CCGC^GG
SbfI         2157  CCTGCAGG             CCTGCA^GG
ScaI          278  AGTACT               AGT^ACT
Sse8387I     2157  CCTGCAGG             CCTGCA^GG
Tsp509I       256  AATT                 ^AATT
Tth111I      1066  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        243  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          262  GTCTAC               GT^[AC][GT]AC
              631  GTCTAC              
AlwI         2526  GATCC                GGATC(4/5)
             2760  GATCC               
AocI         1241  CCTCAGG              CC^T[ACGT]AGG
             2491  CCTGAGG             
AviII         320  TGCGCA               TGC^GCA
             2807  TGCGCA              
BbvCI        2186  CCTCAGC              CCTCAGC(-5/-2)
             1108  GCTGAGG             
BcgI         2436  CGACCCCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              797  GCATGGACTTCG        
Bsp1407I      557  TGTACA               T^GTACA
             2825  TGTACA              
BsrGI         557  TGTACA               T^GTACA
             2825  TGTACA              
BstXI         554  CCATGTACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1403  CCAGCCCCCTGG        
BstYI        2525  AGATCC               [GA]^GATC[CT]
             2759  AGATCC              
Bsu36I       1241  CCTCAGG              CC^T[ACGT]AGG
             2491  CCTGAGG             
DrdI         1063  GACGACTTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1684  GACTTGCACGTC        
Eco81I       1241  CCTCAGG              CC^T[ACGT]AGG
             2491  CCTGAGG             
FspI          320  TGCGCA               TGC^GCA
             2807  TGCGCA              
MflI         2525  AGATCC               [GA]^GATC[CT]
             2759  AGATCC              
MseI          143  TTAA                 T^TAA
             2108  TTAA                
NcoI         2010  CCATGG               C^CATGG
             2171  CCATGG              
SacI         1158  GAGCTC               GAGCT^C
             2401  GAGCTC              
SapI          348  GCTCTTC              GCTCTTC(1/4)
             2348  GCTCTTC             
SfiI         1246  GGCCGGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2059  GGCCCCTTTGGCC       
SmlI         1517  CTCAAG               C^T[CT][GA]AG
             2107  CTTAAG              
SspBI         557  TGTACA               T^GTACA
             2825  TGTACA              
Tru9I         143  TTAA                 T^TAA
             2108  TTAA                
XhoII        2525  AGATCC               [GA]^GATC[CT]
             2759  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SfoI        SmaI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        XmaCI       XmaI        
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