Restriction map for pF1KSDA0722
 (Linear) (Six-base) MAPSORT of: KIAA0722.seq  Check: 126  from: 1  to: 3150



KIAA0722.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1262    1299    2790    3150 
   Size:       1262      37    1491     360

  Fragments arranged by size: 

                1491    1262     360      37
 
AccI GT'mk_AC

Cuts at:      0     263     632    3150 
   Size:        263     369    2518

  Fragments arranged by size: 

                2518     369     263
 
Acc65I G'GTAC_C

Cuts at:      0     509    3150 
   Size:        509    2641
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    2608    2731    2925    3150 
   Size:        220    2388     123     194     225

  Fragments arranged by size: 

                2388     225     220     194     123
 
AfeI AGC'GCT

Cuts at:      0    2875    3150 
   Size:       2875     275
 
AflII C'TTAA_G

Cuts at:      0    2107    3150 
   Size:       2107    1043
 
AleI CACnn'nnGTG

Cuts at:      0    1309    3150 
   Size:       1309    1841
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2987    3150 
   Size:       2987     163
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2971    3003    3150 
   Size:       2971      32     147

  Fragments arranged by size: 

                2971     147      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     174     642     684     969    1923    2398    2856    3150 
   Size:        174     468      42     285     954     475     458     294

  Fragments arranged by size: 

                 954     475     468     458     294     285     174      42
            
 
ApaI G_GGCC'C

Cuts at:      0    2029    2062    2305    2332    2378    2620    3150 
   Size:       2029      33     243      27      46     242     530

  Fragments arranged by size: 

                2029     530     243     242      46      33      27
 
AvaI C'yCGr_G

Cuts at:      0     568     731     891    1022    1621    2139    2277    3150 
   Size:        568     163     160     131     599     518     138     873

  Fragments arranged by size: 

                 873     599     568     518     163     160     138     131
            
 
BanI G'GyrC_C

Cuts at:      0      73     370     509     622     654    1113    1279    2233 
   Size:         73     297     139     113      32     459     166     954

Cuts at:   2233    2367    3150 
   Size:        134     783

  Fragments arranged by size: 

                 954     783     459     297     166     139     134     113
                  73      32
 
BanII G_rGCy'C

Cuts at:      0       8    1162    1813    1867    2029    2062    2223    2272 
   Size:          8    1154     651      54     162      33     161      49

Cuts at:   2272    2305    2332    2378    2405    2620    3150 
   Size:         33      27      46      27     215     530

  Fragments arranged by size: 

                1154     651     530     215     162     161      54      49
                  46      33      33      27      27       8
 
BbeI G_GCGC'C

Cuts at:      0      77     374     658    1117    1283    3150 
   Size:         77     297     284     459     166    1867

  Fragments arranged by size: 

                1867     459     297     284     166      77
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81     982    1909    2250    3150 
   Size:         81     901     927     341     900

  Fragments arranged by size: 

                 927     901     900     341      81
 
BbvCI CC'TCA_GC

Cuts at:      0    1109    2187    3150 
   Size:       1109    1078     963

  Fragments arranged by size: 

                1109    1078     963
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     786     820    2425    2459    3150 
   Size:        786      34    1605      34     691

  Fragments arranged by size: 

                1605     786     691      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      17     991    1253    1412    2066    2336    2605    3150 
   Size:         17     974     262     159     654     270     269     545

  Fragments arranged by size: 

                 974     654     545     270     269     262     159      17
            
 
BlpI GC'TnA_GC

Cuts at:      0     328    1451    2668    3150 
   Size:        328    1123    1217     482

  Fragments arranged by size: 

                1217    1123     482     328
 
Bme1580I G_kGCm'C

Cuts at:      0     878    1886    2029    2062    2236    2305    2332    2378 
   Size:        878    1008     143      33     174      69      27      46

Cuts at:   2378    2620    3150 
   Size:        242     530

  Fragments arranged by size: 

                1008     878     530     242     174     143      69      46
                  33      27
 
BmgBI CAC'GTC

Cuts at:      0    1692    2568    3150 
   Size:       1692     876     582

  Fragments arranged by size: 

                1692     876     582
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1112    1229    1291    1350    3150 
   Size:       1112     117      62      59    1800

  Fragments arranged by size: 

                1800    1112     117      62      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     894    1248    1569    2396    2476    2593    3061 
   Size:        499     395     354     321     827      80     117     468

Cuts at:   3061    3150 
   Size:         89

  Fragments arranged by size: 

                 827     499     468     395     354     321     117      89
                  80
 
Bpu10I CC'TnA_GC

Cuts at:      0    1109    1535    2187    2399    2857    3150 
   Size:       1109     426     652     212     458     293

  Fragments arranged by size: 

                1109     652     458     426     293     212
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1502    3150 
   Size:       1502    1648
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      19     730    2491    3150 
   Size:         19     711    1761     659

  Fragments arranged by size: 

                1761     711     659      19
 
BsaHI Gr'CG_yC

Cuts at:      0      74     371     655    1114    1280    1463    1558    1960 
   Size:         74     297     284     459     166     183      95     402

Cuts at:   1960    3150 
   Size:       1190

  Fragments arranged by size: 

                1190     459     402     297     284     183     166      95
                  74
 
BsaWI w'CCGG_w

Cuts at:      0    1973    3150 
   Size:       1973    1177
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     922     952    1191    1221    1497    1527    3150 
   Size:        922      30     239      30     276      30    1623

  Fragments arranged by size: 

                1623     922     276     239      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1505    1602    2509    3150 
   Size:       1505      97     907     641

  Fragments arranged by size: 

                1505     907     641      97
 
BseYI C'CCAG_C

Cuts at:      0     177     584    1647    1769    1909    2227    2391    3150 
   Size:        177     407    1063     122     140     318     164     759

  Fragments arranged by size: 

                1063     759     407     318     177     164     140     122
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     370     997    1170    1333    1601    1645    2194 
   Size:        295      75     627     173     163     268      44     549

Cuts at:   2194    2581    2974    2983    3150 
   Size:        387     393       9     167

  Fragments arranged by size: 

                 627     549     393     387     295     268     173     167
                 163      75      44       9
 
BsiEI CG_ry'CG

Cuts at:      0      12     453    1197    3150 
   Size:         12     441     744    1953

  Fragments arranged by size: 

                1953     744     441      12
 
BsiHKAI G_wGCw'C

Cuts at:      0    1162    1628    2290    2405    2519    3150 
   Size:       1162     466     662     115     114     631

  Fragments arranged by size: 

                1162     662     631     466     115     114
 
BsmI GAATG_Cn'

Cuts at:      0    2909    3150 
   Size:       2909     241
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     878    1162    1628    1813    1867    1886    2029 
   Size:          8     870     284     466     185      54      19     143

Cuts at:   2029    2062    2223    2236    2272    2290    2305    2332    2378 
   Size:         33     161      13      36      18      15      27      46

Cuts at:   2378    2405    2519    2620    3150 
   Size:         27     114     101     530

  Fragments arranged by size: 

                 870     530     466     284     185     161     143     114
                 101      54      46      36      33      27      27      19
                  18      15      13       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     317     692    1262    1299    1349    1379    1583    1811 
   Size:        317     375     570      37      50      30     204     228

Cuts at:   1811    1931    2108    2790    3150 
   Size:        120     177     682     360

  Fragments arranged by size: 

                 682     570     375     360     317     228     204     177
                 120      50      37      30
 
BsrBI CCG'CTC

Cuts at:      0    1539    1553    2282    3114    3150 
   Size:       1539      14     729     832      36

  Fragments arranged by size: 

                1539     832     729      36      14
 
BsrDI GCAATG_nn'

Cuts at:      0    2441    3150 
   Size:       2441     709
 
BsrFI r'CCGG_y

Cuts at:      0     448    1496    2069    2767    3150 
   Size:        448    1048     573     698     383

  Fragments arranged by size: 

                1048     698     573     448     383
 
BsrGI T'GTAC_A

Cuts at:      0     557    2825    3150 
   Size:        557    2268     325

  Fragments arranged by size: 

                2268     557     325
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     318     969    1274    2777    2805    2961    3057    3104 
   Size:        318     651     305    1503      28     156      96      47

Cuts at:   3104    3150 
   Size:         46

  Fragments arranged by size: 

                1503     651     318     305     156      96      47      46
                  28
 
BstEII G'GTnAC_C

Cuts at:      0    1096    3150 
   Size:       1096    2054
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     561    1410    3150 
   Size:        561     849    1740

  Fragments arranged by size: 

                1740     849     561
 
BstYI r'GATC_y

Cuts at:      0    2525    2759    3150 
   Size:       2525     234     391

  Fragments arranged by size: 

                2525     391     234
 
Bsu36I CC'TnA_GG

Cuts at:      0    1242    2492    3150 
   Size:       1242    1250     658

  Fragments arranged by size: 

                1250    1242     658
 
BtgI C'CryG_G

Cuts at:      0      12      29      69    1191    1721    1779    2010    2171 
   Size:         12      17      40    1122     530      58     231     161

Cuts at:   2171    2264    3150 
   Size:         93     886

  Fragments arranged by size: 

                1122     886     530     231     161      93      58      40
                  17      12
 
BtsI GCAGTG_nn'

Cuts at:      0    1158    2921    3150 
   Size:       1158    1763     229

  Fragments arranged by size: 

                1763    1158     229
 
DraIII CAC_nnn'GTG

Cuts at:      0    2367    2952    3095    3150 
   Size:       2367     585     143      55

  Fragments arranged by size: 

                2367     585     143      55
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1069    1690    3150 
   Size:       1069     621    1460

  Fragments arranged by size: 

                1460    1069     621
 
EaeI y'GGCC_r

Cuts at:      0       9      66     299     450     533    1194    1469    1540 
   Size:          9      57     233     151      83     661     275      71

Cuts at:   1540    2038    2067    2648    2717    3150 
   Size:        498      29     581      69     433

  Fragments arranged by size: 

                 661     581     498     433     275     233     151      83
                  71      69      57      29       9
 
EagI C'GGCC_G

Cuts at:      0       9     450    1194    3150 
   Size:          9     441     744    1956

  Fragments arranged by size: 

                1956     744     441       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     355    1053    2355    3150 
   Size:        355     698    1302     795

  Fragments arranged by size: 

                1302     795     698     355
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     619    1687    3150 
   Size:        619    1068    1463

  Fragments arranged by size: 

                1463    1068     619
 
EcoICRI GAG'CTC

Cuts at:      0    1160    2403    3150 
   Size:       1160    1243     747

  Fragments arranged by size: 

                1243    1160     747
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     499     894    1248    1569    2396    2476    2593 
   Size:        220     279     395     354     321     827      80     117

Cuts at:   2593    2608    2731    2925    3061    3150 
   Size:         15     123     194     136      89

  Fragments arranged by size: 

                 827     395     354     321     279     220     194     136
                 123     117      89      80      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     849    1604    1993    2411    2491    3150 
   Size:        849     755     389     418      80     659

  Fragments arranged by size: 

                 849     755     659     418     389      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57     294     681    1595    2002    2026    2049    2058 
   Size:         57     237     387     914     407      24      23       9

Cuts at:   2058    2059    2301    2302    2329    2375    2388    2478    2616 
   Size:          1     242       1      27      46      13      90     138

Cuts at:   2616    2617    2853    3027    3150 
   Size:          1     236     174     123

  Fragments arranged by size: 

                 914     407     387     242     237     236     174     138
                 123      90      57      46      27      24      23      13
                   9       1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     675    2079    3150 
   Size:        675    1404    1071

  Fragments arranged by size: 

                1404    1071     675
 
FseI GG_CCGG'CC

Cuts at:      0    1500    3150 
   Size:       1500    1650
 
FspI TGC'GCA

Cuts at:      0     322    2809    3150 
   Size:        322    2487     341

  Fragments arranged by size: 

                2487     341     322
 
FspAI rTGC'GCAy

Cuts at:      0     322    3150 
   Size:        322    2828
 
HaeII r_GCGC'y

Cuts at:      0      77     374     658    1117    1283    1670    2600    2877 
   Size:         77     297     284     459     166     387     930     277

Cuts at:   2877    3130    3150 
   Size:        253      20

  Fragments arranged by size: 

                 930     459     387     297     284     277     253     166
                  77      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1191    1223    1950    1982    2713    2745    3150 
   Size:       1191      32     727      32     731      32     405

  Fragments arranged by size: 

                1191     731     727     405      32      32      32
 
KasI G'GCGC_C

Cuts at:      0      73     370     654    1113    1279    3150 
   Size:         73     297     284     459     166    1871

  Fragments arranged by size: 

                1871     459     297     284     166      73
 
KpnI G_GTAC'C

Cuts at:      0     513    3150 
   Size:        513    2637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1503    3150 
   Size:        464    1039    1647

  Fragments arranged by size: 

                1647    1039     464
 
MscI TGG'CCA

Cuts at:      0    1471    3150 
   Size:       1471    1679
 
MslI CAynn'nnrTG

Cuts at:      0     559    1309    2314    3150 
   Size:        559     750    1005     836

  Fragments arranged by size: 

                1005     836     750     559
 
MspA1I CmG'CkG

Cuts at:      0      14     750     759     969    1003    2191    2326    2631 
   Size:         14     736       9     210      34    1188     135     305

Cuts at:   2631    2697    2895    3150 
   Size:         66     198     255

  Fragments arranged by size: 

                1188     736     305     255     210     198     135      66
                  34      14       9
 
NaeI GCC'GGC

Cuts at:      0     450    1498    2769    3150 
   Size:        450    1048    1271     381

  Fragments arranged by size: 

                1271    1048     450     381
 
NarI GG'CG_CC

Cuts at:      0      74     371     655    1114    1280    3150 
   Size:         74     297     284     459     166    1870

  Fragments arranged by size: 

                1870     459     297     284     166      74
 
NcoI C'CATG_G

Cuts at:      0    2010    2171    3150 
   Size:       2010     161     979

  Fragments arranged by size: 

                2010     979     161
 
NgoMIV G'CCGG_C

Cuts at:      0     448    1496    2767    3150 
   Size:        448    1048    1271     383

  Fragments arranged by size: 

                1271    1048     448     383
 
NspI r_CATG'y

Cuts at:      0    3060    3150 
   Size:       3060      90
 
PasI CC'CwG_GG

Cuts at:      0     951    1410    1731    2298    3150 
   Size:        951     459     321     567     852

  Fragments arranged by size: 

                 951     852     567     459     321
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     249    3150 
   Size:        249    2901
 
PfoI T'CCnGG_A

Cuts at:      0     242    2412    2570    2882    2900    3038    3150 
   Size:        242    2170     158     312      18     138     112

  Fragments arranged by size: 

                2170     312     242     158     138     112      18
 
PpuMI rG'GwC_Cy

Cuts at:      0      57     294     681    1595    2002    2049    3150 
   Size:         57     237     387     914     407      47    1101

  Fragments arranged by size: 

                1101     914     407     387     237      57      47
 
PshAI GACnn'nnGTC

Cuts at:      0     715    3150 
   Size:        715    2435
 
PspOMI G'GGCC_C

Cuts at:      0    2025    2058    2301    2328    2374    2616    3150 
   Size:       2025      33     243      27      46     242     534

  Fragments arranged by size: 

                2025     534     243     242      46      33      27
 
PstI C_TGCA'G

Cuts at:      0     359     974    1225    1346    1790    1838    2162    2383 
   Size:        359     615     251     121     444      48     324     221

Cuts at:   2383    2428    2636    2873    3053    3150 
   Size:         45     208     237     180      97

  Fragments arranged by size: 

                 615     444     359     324     251     237     221     208
                 180     121      97      48      45
 
PvuII CAG'CTG

Cuts at:      0     759     969    2191    2631    2697    3150 
   Size:        759     210    1222     440      66     453

  Fragments arranged by size: 

                1222     759     453     440     210      66
 
SacI G_AGCT'C

Cuts at:      0    1162    2405    3150 
   Size:       1162    1243     745

  Fragments arranged by size: 

                1243    1162     745
 
SacII CC_GC'GG

Cuts at:      0      15    3150 
   Size:         15    3135
 
SanDI GG'GwC_CC

Cuts at:      0    2002    3150 
   Size:       2002    1148
 
SapI GCTCTTCn'nnn_

Cuts at:      0     355    2355    3150 
   Size:        355    2000     795

  Fragments arranged by size: 

                2000     795     355
 
SbfI CC_TGCA'GG

Cuts at:      0    2162    3150 
   Size:       2162     988
 
ScaI AGT'ACT

Cuts at:      0     280    3150 
   Size:        280    2870
 
SexAI A'CCwGG_T

Cuts at:      0     266    1100    2363    3150 
   Size:        266     834    1263     787

  Fragments arranged by size: 

                1263     834     787     266
 
SfcI C'TryA_G

Cuts at:      0     355     970    1221    1342    1786    1834    2158    2379 
   Size:        355     615     251     121     444      48     324     221

Cuts at:   2379    2424    2632    2869    3049    3150 
   Size:         45     208     237     180     101

  Fragments arranged by size: 

                 615     444     355     324     251     237     221     208
                 180     121     101      48      45
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1253    2066    3150 
   Size:       1253     813    1084

  Fragments arranged by size: 

                1253    1084     813
 
SfoI GGC'GCC

Cuts at:      0      75     372     656    1115    1281    3150 
   Size:         75     297     284     459     166    1869

  Fragments arranged by size: 

                1869     459     297     284     166      75
 
SmaI CCC'GGG

Cuts at:      0     733    1024    2141    3150 
   Size:        733     291    1117    1009

  Fragments arranged by size: 

                1117    1009     733     291
 
SmlI C'TyrA_G

Cuts at:      0    1517    2107    3150 
   Size:       1517     590    1043

  Fragments arranged by size: 

                1517    1043     590
 
StyI C'CwwG_G

Cuts at:      0    1874    2010    2054    2171    3150 
   Size:       1874     136      44     117     979

  Fragments arranged by size: 

                1874     979     136     117      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     837    1361    1365    1369    1477    1830    2228    3150 
   Size:        837     524       4       4     108     353     398     922

  Fragments arranged by size: 

                 922     837     524     398     353     108       4       4
            
 
TatI w'GTAC_w

Cuts at:      0     278     557    2825    3150 
   Size:        278     279    2268     325

  Fragments arranged by size: 

                2268     325     279     278
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     922     954    1497    1529    3150 
   Size:        922      32     543      32    1621

  Fragments arranged by size: 

                1621     922     543      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1069    3150 
   Size:       1069    2081
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     930    1453    1728    2368    3150 
   Size:        930     523     275     640     782

  Fragments arranged by size: 

                 930     782     640     523     275
 
XmaI C'CCGG_G

Cuts at:      0     731    1022    2139    3150 
   Size:        731     291    1117    1011

  Fragments arranged by size: 

                1117    1011     731     291
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII     AleI     AlfI
     AloI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII
    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI   AflIII     AgeI     AhdI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI    BfrBI
    BglII     BmtI     BplI    BsaAI    BsaBI    BsiWI    BsmBI    BspEI
    BspHI   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI    CspCI
     DraI    EcoRI    EcoRV   HincII  HindIII     HpaI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmnI     ZraI
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