Restriction map for pF1KSDA0696
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BseAI       BseRI       BsiWI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FbaI        FseI        HaeII       HgaI        Hin1I       
HindIII     KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflIII       1479  ACGTGT               A^C[GA][CT]GT
AhdI          241  GACCAGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         241  GACCAGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1427  CTCGAG               C^[CT]CG[GA]G
BalI         1080  TGGCCA               TGG^CCA
BanI         1297  GGTGCC               G^G[CT][GA]CC
BcnI         1086  CCGGG                CC^[GC]GG
BsaWI        1346  TCCGGT               [AT]^CCGG[AT]
BsgI          434  GTGCAG               GTGCAG(16/14)
BsiEI        1049  CGACCG               CG[GA][CT]^CG
BsmBI         174  CGTCTC               CGTCTC(1/5)
BsmI          482  GAATGC               GAATGC(1/-1)
BsoBI        1427  CTCGAG               C^[CT]CG[GA]G
BssSI        1167  CACGAG               CACGAG(-5/-1)
BstAPI        117  GCAGAGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        999  GGTGACC              G^GT[ACGT]ACC
BstPI         999  GGTGACC              G^GT[ACGT]ACC
DraI          574  TTTAAA               TTT^AAA
DraII        1375  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1443  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1080  TGGCCA               [CT]^GGCC[GA]
Eam1105I      241  GACCAGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1375  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       999  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1341  ATGCAT               ATGCA^T
HincII        825  GTTAAC               GT[CT]^[GA]AC
HindII        825  GTTAAC               GT[CT]^[GA]AC
HpaI          825  GTTAAC               GTT^AAC
MaeII        1479  ACGT                 A^CGT
MluNI        1080  TGGCCA               TGG^CCA
MscI         1080  TGGCCA               TGG^CCA
MspA1I        346  CAGCGG               C[AC]G^C[GT]G
NciI         1086  CCGGG                CC^[GC]GG
NsiI         1341  ATGCAT               ATGCA^T
PaeR7I       1427  CTCGAG               C^TCGAG
SacI           37  GAGCTC               GAGCT^C
SapI          887  GCTCTTC              GCTCTTC(1/4)
SexAI          83  ACCTGGT              A^CC[AT]GGT
SmlI         1427  CTCGAG               C^T[CT][GA]AG
SphI          122  GCATGC               GCATG^C
SspI          233  AATATT               AAT^ATT
XbaI         1621  TCTAGA               T^CTAGA
XhoI         1427  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          727  GTCTAC               GT^[AC][GT]AC
             1105  GTAGAC              
AlwNI         565  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              839  CAGGCTCTG           
Asp700I       397  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1180  GAATTTGTTC          
AviII         100  TGCGCA               TGC^GCA
             1454  TGCGCA              
BsaI         1585  GAGACC               GGTCTC(1/5)
              425  GGTCTC              
BsaJI          55  CCAAGG               C^C[ACGT][ACGT]GG
             1010  CCAAGG              
BsrBI         705  CCGCTC               CCGCTC(-3/-3)
             1017  CCGCTC              
EarI          888  CTCTTC               CTCTTC(1/4)
              551  GAAGAG              
EcoT14I        55  CCAAGG               C^C[AT][AT]GG
             1010  CCAAGG              
FauI          348  GCGGG                CCCGC(4/6)
             1209  GCGGG               
FspI          100  TGCGCA               TGC^GCA
             1454  TGCGCA              
HapII        1086  CCGG                 C^CGG
             1347  CCGG                
HpaII        1086  CCGG                 C^CGG
             1347  CCGG                
Ksp632I       888  CTCTTC               CTCTTC(1/4)
              551  GAAGAG              
MspI         1086  CCGG                 C^CGG
             1347  CCGG                
NspI           95  GCATGT               [GA]CATG^[CT]
              122  GCATGC              
PleI           10  GACTC                GAGTC(4/5)
             1310  GAGTC               
PstI          115  CTGCAG               CTGCA^G
              856  CTGCAG              
StyI           55  CCAAGG               C^C[AT][AT]GG
             1010  CCAAGG              
TaqI           35  TCGA                 T^CGA
             1428  TCGA                
Tsp45I       1000  GTGAC                ^GT[GC]AC
             1142  GTGAC               
TthHB8I        35  TCGA                 T^CGA
             1428  TCGA                
XcmI           55  CCAAGGTCTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              144  CCAGATAAGTAATGG     
XmnI          397  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1180  GAATTTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbvI        
BfaI        BmyI        BpmI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       
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