Restriction map for pF1KSDA0694
 (Linear) (Six-base) MAPSORT of: KIAA0694.seq  Check: 7721  from: 1  to: 1779



KIAA0694.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AclI AA'CG_TT

Cuts at:      0    1755    1779 
   Size:       1755      24
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     968    1198    1728    1779 
   Size:        311     657     230     530      51

  Fragments arranged by size: 

                 657     530     311     230      51
 
AfeI AGC'GCT

Cuts at:      0     625    1779 
   Size:        625    1154
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1576    1608    1779 
   Size:       1576      32     171

  Fragments arranged by size: 

                1576     171      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     289    1779 
   Size:        289    1490
 
ApaI G_GGCC'C

Cuts at:      0     887    1779 
   Size:        887     892
 
ApoI r'AATT_y

Cuts at:      0     315     340     573    1465    1779 
   Size:        315      25     233     892     314

  Fragments arranged by size: 

                 892     315     314     233      25
 
AvaI C'yCGr_G

Cuts at:      0     142     413    1779 
   Size:        142     271    1366

  Fragments arranged by size: 

                1366     271     142
 
AvrII C'CTAG_G

Cuts at:      0     133    1779 
   Size:        133    1646
 
BamHI G'GATC_C

Cuts at:      0     842    1779 
   Size:        842     937
 
BanI G'GyrC_C

Cuts at:      0    1564    1779 
   Size:       1564     215
 
BanII G_rGCy'C

Cuts at:      0      65     887     914    1779 
   Size:         65     822      27     865

  Fragments arranged by size: 

                 865     822      65      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     500    1779 
   Size:        500    1279
 
BclI T'GATC_A

Cuts at:      0    1145    1184    1779 
   Size:       1145      39     595

  Fragments arranged by size: 

                1145     595      39
 
BfrBI ATG'CAT

Cuts at:      0     713    1779 
   Size:        713    1066
 
BglI GCCn_nnn'nGGC

Cuts at:      0      94    1779 
   Size:         94    1685
 
BglII A'GATC_T

Cuts at:      0     210    1122    1779 
   Size:        210     912     657

  Fragments arranged by size: 

                 912     657     210
 
Bme1580I G_kGCm'C

Cuts at:      0     887    1779 
   Size:        887     892
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     221     348    1779 
   Size:        221     127    1431

  Fragments arranged by size: 

                1431     221     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413    1779 
   Size:        413    1366
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      36    1779 
   Size:         36    1743
 
BsaWI w'CCGG_w

Cuts at:      0     191    1779 
   Size:        191    1588
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1395    1425    1576    1606    1779 
   Size:       1395      30     151      30     173

  Fragments arranged by size: 

                1395     173     151      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     539     912     915    1779 
   Size:        539     373       3     864

  Fragments arranged by size: 

                 864     539     373       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     763    1779 
   Size:        763    1016
 
BsiEI CG_ry'CG

Cuts at:      0      59    1779 
   Size:         59    1720
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     305     907     914    1779 
   Size:         65     240     602       7     865

  Fragments arranged by size: 

                 865     602     240      65       7
 
BsmI GAATG_Cn'

Cuts at:      0     860     964    1779 
   Size:        860     104     815

  Fragments arranged by size: 

                 860     815     104
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     305     887     907     914    1779 
   Size:         65     240     582      20       7     865

  Fragments arranged by size: 

                 865     582     240      65      20       7
 
BspHI T'CATG_A

Cuts at:      0    1181    1779 
   Size:       1181     598
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      11    1779 
   Size:         11    1768
 
BsrBI CCG'CTC

Cuts at:      0      12     529    1779 
   Size:         12     517    1250

  Fragments arranged by size: 

                1250     517      12
 
BsrDI GCAATG_nn'

Cuts at:      0    1553    1779 
   Size:       1553     226
 
BsrFI r'CCGG_y

Cuts at:      0       4     107    1779 
   Size:          4     103    1672

  Fragments arranged by size: 

                1672     103       4
 
BsrGI T'GTAC_A

Cuts at:      0     730    1779 
   Size:        730    1049
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1009    1564    1779 
   Size:       1009     555     215

  Fragments arranged by size: 

                1009     555     215
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1457    1779 
   Size:       1457     322
 
BstYI r'GATC_y

Cuts at:      0     210     842    1086    1122    1332    1779 
   Size:        210     632     244      36     210     447

  Fragments arranged by size: 

                 632     447     244     210     210      36
 
Bsu36I CC'TnA_GG

Cuts at:      0     878    1779 
   Size:        878     901
 
BtgI C'CryG_G

Cuts at:      0      89    1779 
   Size:         89    1690
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     501     536    1779 
   Size:        501      35    1243

  Fragments arranged by size: 

                1243     501      35
 
EaeI y'GGCC_r

Cuts at:      0       2      56    1523    1779 
   Size:          2      54    1467     256

  Fragments arranged by size: 

                1467     256      54       2
 
EagI C'GGCC_G

Cuts at:      0      56    1779 
   Size:         56    1723
 
EarI CTCTTCn'nnn_

Cuts at:      0     209    1779 
   Size:        209    1570
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      72    1779 
   Size:         72    1707
 
EcoICRI GAG'CTC

Cuts at:      0      63     912    1779 
   Size:         63     849     867

  Fragments arranged by size: 

                 867     849      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     413     968    1198    1728    1779 
   Size:        311     102     555     230     530      51

  Fragments arranged by size: 

                 555     530     311     230     102      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     883     884    1779 
   Size:        883       1     895

  Fragments arranged by size: 

                 895     883       1
 
EcoRV GAT'ATC

Cuts at:      0     264    1779 
   Size:        264    1515
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     645    1779 
   Size:        645    1134
 
HaeII r_GCGC'y

Cuts at:      0      77     627    1779 
   Size:         77     550    1152

  Fragments arranged by size: 

                1152     550      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     676     708    1056    1088    1346    1378    1779 
   Size:        676      32     348      32     258      32     401

  Fragments arranged by size: 

                 676     401     348     258      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1692    1779 
   Size:       1692      87
 
HindIII A'AGCT_T

Cuts at:      0     436    1740    1779 
   Size:        436    1304      39

  Fragments arranged by size: 

                1304     436      39
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1619    1649    1779 
   Size:       1619      30     130

  Fragments arranged by size: 

                1619     130      30
 
MscI TGG'CCA

Cuts at:      0    1525    1779 
   Size:       1525     254
 
MslI CAynn'nnrTG

Cuts at:      0     470    1779 
   Size:        470    1309
 
MspA1I CmG'CkG

Cuts at:      0      91     120     636     814    1779 
   Size:         91      29     516     178     965

  Fragments arranged by size: 

                 965     516     178      91      29
 
NaeI GCC'GGC

Cuts at:      0     109    1779 
   Size:        109    1670
 
NgoMIV G'CCGG_C

Cuts at:      0     107    1779 
   Size:        107    1672
 
NsiI A_TGCA'T

Cuts at:      0     715    1779 
   Size:        715    1064
 
NspI r_CATG'y

Cuts at:      0     364    1779 
   Size:        364    1415
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1576    1608    1779 
   Size:       1576      32     171

  Fragments arranged by size: 

                1576     171      32
 
PspOMI G'GGCC_C

Cuts at:      0     883    1779 
   Size:        883     896
 
PstI C_TGCA'G

Cuts at:      0    1642    1779 
   Size:       1642     137
 
PvuII CAG'CTG

Cuts at:      0     636     814    1779 
   Size:        636     178     965

  Fragments arranged by size: 

                 965     636     178
 
SacI G_AGCT'C

Cuts at:      0      65     914    1779 
   Size:         65     849     865

  Fragments arranged by size: 

                 865     849      65
 
SacII CC_GC'GG

Cuts at:      0      92    1779 
   Size:         92    1687
 
SfcI C'TryA_G

Cuts at:      0    1638    1779 
   Size:       1638     141
 
SmlI C'TyrA_G

Cuts at:      0     142    1779 
   Size:        142    1637
 
SspI AAT'ATT

Cuts at:      0     388    1257    1779 
   Size:        388     869     522

  Fragments arranged by size: 

                 869     522     388
 
StyI C'CwwG_G

Cuts at:      0     133     512     891    1779 
   Size:        133     379     379     888

  Fragments arranged by size: 

                 888     379     379     133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     599    1779 
   Size:        599    1180
 
TatI w'GTAC_w

Cuts at:      0     275     730    1779 
   Size:        275     455    1049

  Fragments arranged by size: 

                1049     455     275
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     493     525    1395    1427    1779 
   Size:        493      32     870      32     352

  Fragments arranged by size: 

                 870     493     352      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1083    1779 
   Size:       1083     696
 
XhoI C'TCGA_G

Cuts at:      0     142    1779 
   Size:        142    1637
 

 Enzymes that do cut:

     AclI     AcuI     AfeI     AloI    AlwNI     ApaI     ApoI     AvaI
    AvrII    BamHI     BanI    BanII     BbsI     BclI    BfrBI     BglI
    BglII Bme1580I     BmrI     BpmI     BsaI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    CspCI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PpiI   PspOMI
     PstI    PvuII     SacI    SacII     SfcI     SmlI     SspI     StyI
    TaqII     TatI     TstI     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I    AflII   AflIII     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI     AseI    AsiSI     BaeI     BbeI
    BbvCI     BcgI    BciVI     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BseYI    BsiWI    BsmBI    BspEI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI    EcoNI    EcoRI     FseI     FspI    FspAI
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI  Tth111I     XcmI     XmaI     XmnI     ZraI
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