Restriction map for pF1KSDA0685
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       BbrPI       
BbsI        BcgI        BciVI       BclI        BfrI        
BglII       BpuAI       BsaAI       BsaBI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoRI       
EcoRV       FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tsp509I     
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AatII         541  GACGTC               GACGT^C
AspHI        1090  GAGCTC               G[AT]GC[AT]^C
AvrII        2682  CCTAGG               C^CTAGG
BfaI         2683  CTAG                 C^TAG
BlnI         2682  CCTAGG               C^CTAGG
BlpI          306  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      306  GCTGAGC              GC^T[ACGT]AGC
BsiHKAI      1090  GAGCTC               G[AT]GC[AT]^C
BspLU11I      761  ACATGT               A^CATGT
BsrDI         393  CATTGC               GCAATG(2/0)
BstAPI       2410  GCAGCGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1326  GGTGACC              G^GT[ACGT]ACC
BstPI        1326  GGTGACC              G^GT[ACGT]ACC
CelII         306  GCTGAGC              GC^T[ACGT]AGC
EcoNI        1981  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1326  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1179  ATGCAT               ATGCA^T
HincII         37  GTTGAC               GT[CT]^[GA]AC
HindII         37  GTTGAC               GT[CT]^[GA]AC
MaeI         2683  CTAG                 C^TAG
NsiI         1179  ATGCAT               ATGCA^T
PpuMI        2439  GGGTCCC              [GA]G^G[AT]CC[CT]
SacI         1090  GAGCTC               GAGCT^C


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII        1455  CGCG                 CG^CG
             2544  CGCG                
AflIII        761  ACATGT               A^C[GA][CT]GT
             2324  ACGTGT              
Aor51HI       480  AGCGCT               AGC^GCT
              720  AGCGCT              
Asp700I       936  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2289  GAAAGCCTTC          
BbeI         1036  GGCGCC               GGCGC^C
             2050  GGCGCC              
BsaI          668  GAGACC               GGTCTC(1/5)
              773  GAGACC              
BsmI         1020  GAATGC               GAATGC(1/-1)
             2248  GAATGC              
BspMI         491  ACCTGC               ACCTGC(4/8)
             1987  GCAGGT              
BsrBI         709  CCGCTC               CCGCTC(-3/-3)
             1474  GAGCGG              
BstUI        1455  CGCG                 CG^CG
             2544  CGCG                
EagI         1298  CGGCCG               C^GGCCG
             2445  CGGCCG              
EarI         2655  CTCTTC               CTCTTC(1/4)
              562  GAAGAG              
EclXI        1298  CGGCCG               C^GGCCG
             2445  CGGCCG              
Eco47III      480  AGCGCT               AGC^GCT
              720  AGCGCT              
Eco52I       1298  CGGCCG               C^GGCCG
             2445  CGGCCG              
KasI         1036  GGCGCC               G^GCGCC
             2050  GGCGCC              
Ksp632I      2655  CTCTTC               CTCTTC(1/4)
              562  GAAGAG              
MvnI         1455  CGCG                 CG^CG
             2544  CGCG                
NarI         1036  GGCGCC               GG^CGCC
             2050  GGCGCC              
NcoI         2420  CCATGG               C^CATGG
             2717  CCATGG              
NspI           60  GCATGT               [GA]CATG^[CT]
              761  ACATGT              
PflMI        1927  CCAGAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2129  CCAGTGTGTGG         
PstI           70  CTGCAG               CTGCA^G
             1785  CTGCAG              
SfcI           70  CTGCAG               C^T[GA][CT]AG
             1785  CTGCAG              
SfoI         1036  GGCGCC               GGC^GCC
             2050  GGCGCC              
SmaI         1862  CCCGGG               CCC^GGG
             2005  CCCGGG              
StuI         1373  AGGCCT               AGG^CCT
             1778  AGGCCT              
TaqI          923  TCGA                 T^CGA
             1739  TCGA                
TthHB8I       923  TCGA                 T^CGA
             1739  TCGA                
Van91I       1927  CCAGAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2129  CCAGTGTGTGG         
XcmI          992  CCATTGGTGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2385  CCACAGCGAGGATGG     
XmaCI        1862  CCCGGG               C^CCGGG
             2005  CCCGGG              
XmaI         1862  CCCGGG               C^CCGGG
             2005  CCCGGG              
XmnI          936  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2289  GAAAGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       ApaI        AvaI        AvaII       BalI        
BamHI       BanI        BanII       BbvCI       BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        Tru9I       TseI        
Tsp45I      TspRI       XhoII       
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