Restriction map for
pF1KSDA0685
(Circular) (Six-base) MAPSORT of: pF1KSDA0685.seq Check: 6504 from: 1 to: 5920
pF1KSDA0685 5920 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2104 2104
Size: 5920
AatII G_ACGT'C
Cuts at: 671 671
Size: 5920
AccI GT'mk_AC
Cuts at: 2956 2956
Size: 5920
Acc65I G'GTAC_C
Cuts at: 2934 2934
Size: 5920
AclI AA'CG_TT
Cuts at: 5760 5760
Size: 5920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1081 2012 2406 3743 4175 4924 5192 901
Size: 180 931 394 1337 432 749 268 1629
Fragments arranged by size:
1629 1337 931 749 432 394 268 180
AfeI AGC'GCT
Cuts at: 608 848 608
Size: 240 5680
AflIII A'CryG_T
Cuts at: 887 2450 3270 4376 5353 887
Size: 1563 820 1106 977 1454
Fragments arranged by size:
1563 1454 1106 977 820
AgeI A'CCGG_T
Cuts at: 4308 4308
Size: 5920
AleI CACnn'nnGTG
Cuts at: 1450 1450
Size: 5920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 770 826 770
Size: 56 5864
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 139
Size: 32 5888
AlwNI CAG_nnn'CTG
Cuts at: 255 879 1005 1080 1596 3223 4792 255
Size: 624 126 75 516 1627 1569 1383
Fragments arranged by size:
1627 1569 1383 624 516 126 75
ApaI G_GGCC'C
Cuts at: 1609 2348 2816 1609
Size: 739 468 4713
Fragments arranged by size:
4713 739 468
ApaLI G'TGCA_C
Cuts at: 4690 4690
Size: 5920
ApoI r'AATT_y
Cuts at: 2922 5273 5529 2922
Size: 2351 256 3313
Fragments arranged by size:
3313 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5920
AvaI C'yCGr_G
Cuts at: 726 1988 2131 2713 2938 726
Size: 1262 143 582 225 3708
Fragments arranged by size:
3708 1262 582 225 143
AvrII C'CTAG_G
Cuts at: 2808 2808
Size: 5920
BamHI G'GATC_C
Cuts at: 315 1360 1490 2943 315
Size: 1045 130 1453 3292
Fragments arranged by size:
3292 1453 1045 130
BanI G'GyrC_C
Cuts at: 598 1162 2116 2176 2272 2934 3348 3596 3631
Size: 564 954 60 96 662 414 248 35
Cuts at: 3631 598
Size: 2887
Fragments arranged by size:
2887 954 662 564 414 248 96 60
35
BanII G_rGCy'C
Cuts at: 659 1139 1220 1609 1646 2019 2348 2509 2816
Size: 480 81 389 37 373 329 161 307
Cuts at: 2816 2932 3962 659
Size: 116 1030 2617
Fragments arranged by size:
2617 1030 480 389 373 329 307 161
116 81 37
BbeI G_GCGC'C
Cuts at: 1166 2180 3352 3600 1166
Size: 1014 1172 248 3486
Fragments arranged by size:
3486 1172 1014 248
BbvCI CC'TCA_GC
Cuts at: 603 2277 2387 603
Size: 1674 110 4136
Fragments arranged by size:
4136 1674 110
BciVI GTATCCnnnnn_n'
Cuts at: 3816 4579 5920 3816
Size: 763 1341 3816
Fragments arranged by size:
3816 1341 763
BclI T'GATC_A
Cuts at: 3438 3438
Size: 5920
BfrBI ATG'CAT
Cuts at: 1307 1307
Size: 5920
BglI GCCn_nnn'nGGC
Cuts at: 654 2125 2848 654
Size: 1471 723 3726
Fragments arranged by size:
3726 1471 723
BglII A'GATC_T
Cuts at: 3433 5472 3433
Size: 2039 3881
BlpI GC'TnA_GC
Cuts at: 433 3030 433
Size: 2597 3323
Bme1580I G_kGCm'C
Cuts at: 1609 2121 2348 2711 2816 3543 3636 4694 1609
Size: 512 227 363 105 727 93 1058 2835
Fragments arranged by size:
2835 1058 727 512 363 227 105 93
BmgBI CAC'GTC
Cuts at: 155 1546 2247 155
Size: 1391 701 3828
Fragments arranged by size:
3828 1391 701
BmrI ACTGGGnnnn_n'
Cuts at: 3130 3211 3546 5692 3130
Size: 81 335 2146 3358
Fragments arranged by size:
3358 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2055 2181 4285 2055
Size: 126 2104 3690
Fragments arranged by size:
3690 2104 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 871 1150 1429 1516 1618 2267 844
Size: 27 279 279 87 102 649 4497
Fragments arranged by size:
4497 649 279 279 102 87 27
Bpu10I CC'TnA_GC
Cuts at: 603 2277 2387 603
Size: 1674 110 4136
Fragments arranged by size:
4136 1674 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3094 3973 4467 4765 3094
Size: 879 494 298 4249
Fragments arranged by size:
4249 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 788 893 5624 788
Size: 105 4731 1084
Fragments arranged by size:
4731 1084 105
BsaAI yAC'GTr
Cuts at: 2910 3271 2910
Size: 361 5559
BsaBI GATnn'nnATC
Cuts at: 6 3432 5471 6
Size: 3426 2039 455
Fragments arranged by size:
3426 2039 455
BsaHI Gr'CG_yC
Cuts at: 668 1163 2177 3349 3597 4301 5797 668
Size: 495 1014 1172 248 704 1496 791
Fragments arranged by size:
1496 1172 1014 791 704 495 248
BsaWI w'CCGG_w
Cuts at: 902 1400 2361 3107 3628 4308 4582 4729 5140
Size: 498 961 746 521 680 274 147 411
Cuts at: 5140 902
Size: 1682
Fragments arranged by size:
1682 961 746 680 521 498 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2265 2295 2479 2509 2265
Size: 30 184 30 5676
Fragments arranged by size:
5676 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 349 829 1147 1354 1699 1810 2302 2487
Size: 45 480 318 207 345 111 492 185
Cuts at: 2487 2626 304
Size: 139 3598
Fragments arranged by size:
3598 492 480 345 318 207 185 139
111 45
BseYI C'CCAG_C
Cuts at: 786 1201 1418 1667 1885 2297 2694 2772 2838
Size: 415 217 249 218 412 397 78 66
Cuts at: 2838 3343 4101 4680 5176 786
Size: 505 758 579 496 1530
Fragments arranged by size:
1530 758 579 505 496 415 412 397
249 218 217 78 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 658 1171 1504 1609 1633 2373 2771 291
Size: 367 513 333 105 24 740 398 3440
Fragments arranged by size:
3440 740 513 398 367 333 105 24
BsiEI CG_ry'CG
Cuts at: 104 408 1427 2574 2621 3460 3506 4716 104
Size: 304 1019 1147 47 839 46 1210 1308
Fragments arranged by size:
1308 1210 1147 1019 839 304 47 46
BsiHKAI G_wGCw'C
Cuts at: 1220 2932 3710 3900 4694 1220
Size: 1712 778 190 794 2446
Fragments arranged by size:
2446 1712 794 778 190
BsmI GAATG_Cn'
Cuts at: 1152 2380 1152
Size: 1228 4692
BsmBI CGTCTCn'nnnn_
Cuts at: 1536 1681 1693 1536
Size: 145 12 5763
Fragments arranged by size:
5763 145 12
Bsp1286I G_dGCh'C
Cuts at: 659 1139 1220 1609 1646 2019 2121 2348 2509
Size: 480 81 389 37 373 102 227 161
Cuts at: 2509 2711 2816 2932 3543 3636 3710 3900 3962
Size: 202 105 116 611 93 74 190 62
Cuts at: 3962 4694 659
Size: 732 1885
Fragments arranged by size:
1885 732 611 480 389 373 227 202
190 161 116 105 102 93 81 74
62 37
BspEI T'CCGG_A
Cuts at: 5140 5140
Size: 5920
BspHI T'CATG_A
Cuts at: 5096 5096
Size: 5920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 626 2104 2968 3182 3484 3865 626
Size: 1478 864 214 302 381 2681
Fragments arranged by size:
2681 1478 864 381 302 214
BsrBI CCG'CTC
Cuts at: 50 837 1602 4210 4264 5750 5915 50
Size: 787 765 2608 54 1486 165 55
Fragments arranged by size:
2608 1486 787 765 165 55 54
BsrDI GCAATG_nn'
Cuts at: 518 3830 518
Size: 3312 2608
BsrFI r'CCGG_y
Cuts at: 1223 1422 2124 2645 3916 4097 4308 1223
Size: 199 702 521 1271 181 211 2835
Fragments arranged by size:
2835 1271 702 521 211 199 181
BssSI C'ACGA_G
Cuts at: 4189 4549 4189
Size: 360 5560
BstAPI GCAn_nnn'nTGC
Cuts at: 2542 3295 2542
Size: 753 5167
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5920
BstEII G'GTnAC_C
Cuts at: 1452 1452
Size: 5920
BstXI CCAn_nnnn'nTGG
Cuts at: 1584 1770 2444 1584
Size: 186 674 5060
Fragments arranged by size:
5060 674 186
BstYI r'GATC_y
Cuts at: 1 315 1360 1490 1745 2943 3410 3433 3768
Size: 314 1045 130 255 1198 467 23 335
Cuts at: 3768 4014 5028 5114 5126 5472 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1198 1045 1014 467 449 346 335 314
255 246 130 86 23 12
BtgI C'CryG_G
Cuts at: 1659 2546 2699 2795 2843 1659
Size: 887 153 96 48 4736
Fragments arranged by size:
4736 887 153 96 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3868 4049 4212 3868
Size: 181 163 5576
Fragments arranged by size:
5576 181 163
BtsI GCAGTG_nn'
Cuts at: 286 1180 1920 2198 3161 286
Size: 894 740 278 963 3045
Fragments arranged by size:
3045 963 894 740 278
DraI TTT'AAA
Cuts at: 2917 2917
Size: 5920
DrdI GACnn_nn'nnGTC
Cuts at: 3624 4484 3624
Size: 860 5060
EaeI y'GGCC_r
Cuts at: 1424 1662 1997 2571 2600 2726 2792 2849 3503
Size: 238 335 574 29 126 66 57 654
Cuts at: 3503 3677 4068 4095 5442 1424
Size: 174 391 27 1347 1902
Fragments arranged by size:
1902 1347 654 574 391 335 238 174
126 66 57 29 27
EagI C'GGCC_G
Cuts at: 1424 2571 3503 1424
Size: 1147 932 3841
Fragments arranged by size:
3841 1147 932
EarI CTCTTCn'nnn_
Cuts at: 683 2787 3941 4151 683
Size: 2104 1154 210 2452
Fragments arranged by size:
2452 2104 1154 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 308 4126 4438 4584 5730 308
Size: 3818 312 146 1146 498
Fragments arranged by size:
3818 1146 498 312 146
EcoICRI GAG'CTC
Cuts at: 1218 2930 1218
Size: 1712 4208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 871 901 1081 1150 1429 1516 1618 2012
Size: 27 30 180 69 279 87 102 394
Cuts at: 2012 2267 2406 3743 4175 4924 5192 844
Size: 255 139 1337 432 749 268 1572
Fragments arranged by size:
1572 1337 749 432 394 279 268 255
180 139 102 87 69 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2111 2111
Size: 5920
EcoO109I rG'GnC_Cy
Cuts at: 1605 1606 2344 2407 2469 2566 2580 2812 3057
Size: 1 738 63 62 97 14 232 245
Cuts at: 3057 1605
Size: 4468
Fragments arranged by size:
4468 738 245 232 97 63 62 14
1
EcoRI G'AATT_C
Cuts at: 2922 2922
Size: 5920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 159 363 2100 3937 159
Size: 204 1737 1837 2142
Fragments arranged by size:
2142 1837 1737 204
FspI TGC'GCA
Cuts at: 3699 3699
Size: 5920
HaeII r_GCGC'y
Cuts at: 610 850 1166 2180 3352 3600 4624 5603 610
Size: 240 316 1014 1172 248 1024 979 927
Fragments arranged by size:
1172 1024 1014 979 927 316 248 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 733 765 2479 2511 2550 2582 3760 3790 3792
Size: 32 1714 32 39 32 1178 30 2
Cuts at: 3792 3822 733
Size: 30 2831
Fragments arranged by size:
2831 1714 1178 39 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 165 2957 165
Size: 2792 3128
KasI G'GCGC_C
Cuts at: 1162 2176 3348 3596 1162
Size: 1014 1172 248 3486
Fragments arranged by size:
3486 1172 1014 248
KpnI G_GTAC'C
Cuts at: 2938 2938
Size: 5920
MluI A'CGCG_T
Cuts at: 5353 5353
Size: 5920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2343 4591 4775 2343
Size: 2248 184 3488
Fragments arranged by size:
3488 2248 184
MscI TGG'CCA
Cuts at: 1999 2602 2728 2851 3679 1999
Size: 603 126 123 828 4240
Fragments arranged by size:
4240 828 603 126 123
MslI CAynn'nnrTG
Cuts at: 1123 1450 2099 3907 4034 1123
Size: 327 649 1808 127 3009
Fragments arranged by size:
3009 1808 649 327 127
MspA1I CmG'CkG
Cuts at: 638 810 1358 1531 1667 2269 2589 3029 3343
Size: 172 548 173 136 602 320 440 314
Cuts at: 3343 3703 4718 4963 638
Size: 360 1015 245 1595
Fragments arranged by size:
1595 1015 602 548 440 360 320 314
245 173 172 136
NaeI GCC'GGC
Cuts at: 1225 1424 2647 4099 1225
Size: 199 1223 1452 3046
Fragments arranged by size:
3046 1452 1223 199
NarI GG'CG_CC
Cuts at: 1163 2177 3349 3597 1163
Size: 1014 1172 248 3486
Fragments arranged by size:
3486 1172 1014 248
NcoI C'CATG_G
Cuts at: 2546 2843 2546
Size: 297 5623
NgoMIV G'CCGG_C
Cuts at: 1223 1422 2645 4097 1223
Size: 199 1223 1452 3046
Fragments arranged by size:
3046 1452 1223 199
NruI TCG'CGA
Cuts at: 4281 4281
Size: 5920
NsiI A_TGCA'T
Cuts at: 1309 1309
Size: 5920
NspI r_CATG'y
Cuts at: 190 891 2971 3471 4380 190
Size: 701 2080 500 909 1730
Fragments arranged by size:
2080 1730 909 701 500
PasI CC'CwG_GG
Cuts at: 1140 2856 1140
Size: 1716 4204
PciI A'CATG_T
Cuts at: 887 4376 887
Size: 3489 2431
PflMI CCAn_nnn'nTGG
Cuts at: 131 2059 2261 131
Size: 1928 202 3790
Fragments arranged by size:
3790 1928 202
PfoI T'CCnGG_A
Cuts at: 1493 1493
Size: 5920
PmeI GTTT'AAAC
Cuts at: 2917 2917
Size: 5920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5059 5091 5059
Size: 32 5888
PpuMI rG'GwC_Cy
Cuts at: 2566 2566
Size: 5920
PspOMI G'GGCC_C
Cuts at: 1605 2344 2812 1605
Size: 739 468 4713
Fragments arranged by size:
4713 739 468
PstI C_TGCA'G
Cuts at: 200 1915 2965 3650 200
Size: 1715 1050 685 2470
Fragments arranged by size:
2470 1715 1050 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5920
PvuII CAG'CTG
Cuts at: 638 810 2269 3343 3703 638
Size: 172 1459 1074 360 2855
Fragments arranged by size:
2855 1459 1074 360 172
RsrII CG'GwC_CG
Cuts at: 4113 4113
Size: 5920
SacI G_AGCT'C
Cuts at: 1220 2932 1220
Size: 1712 4208
SalI G'TCGA_C
Cuts at: 2955 2955
Size: 5920
SanDI GG'GwC_CC
Cuts at: 2566 2566
Size: 5920
SapI GCTCTTCn'nnn_
Cuts at: 3941 4151 3941
Size: 210 5710
SbfI CC_TGCA'GG
Cuts at: 2965 2965
Size: 5920
ScaI AGT'ACT
Cuts at: 5437 5437
Size: 5920
SfcI C'TryA_G
Cuts at: 33 196 1911 2961 3646 4641 4832 33
Size: 163 1715 1050 685 995 191 1121
Fragments arranged by size:
1715 1121 1050 995 685 191 163
SfoI GGC'GCC
Cuts at: 1164 2178 3350 3598 1164
Size: 1014 1172 248 3486
Fragments arranged by size:
3486 1172 1014 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5920
SmaI CCC'GGG
Cuts at: 1990 2133 2940 1990
Size: 143 807 4970
Fragments arranged by size:
4970 807 143
SmlI C'TyrA_G
Cuts at: 3073 3988 4482 4744 3073
Size: 915 494 262 4249
Fragments arranged by size:
4249 915 494 262
SnaBI TAC'GTA
Cuts at: 2910 2910
Size: 5920
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5920
SphI G_CATG'C
Cuts at: 2971 3471 2971
Size: 500 5420
StuI AGG'CCT
Cuts at: 1501 1906 1501
Size: 405 5515
StyI C'CwwG_G
Cuts at: 2403 2546 2808 2843 3052 3406 2403
Size: 143 262 35 209 354 4917
Fragments arranged by size:
4917 354 262 209 143 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1215 3501 3634 4093 4304 1215
Size: 2286 133 459 211 2831
Fragments arranged by size:
2831 2286 459 211 133
TatI w'GTAC_w
Cuts at: 1265 5435 1265
Size: 4170 1750
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 253 285 300 332 2265 2297 2439 2471 5437
Size: 32 15 32 1933 32 142 32 2966
Cuts at: 5437 5469 253
Size: 32 704
Fragments arranged by size:
2966 1933 704 142 32 32 32 32
32 15
Tth111I GACn'n_nGTC
Cuts at: 3715 3715
Size: 5920
XbaI T'CTAG_A
Cuts at: 2949 2949
Size: 5920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1125 2518 3413 1125
Size: 1393 895 3632
Fragments arranged by size:
3632 1393 895
XmaI C'CCGG_G
Cuts at: 1988 2131 2938 1988
Size: 143 807 4970
Fragments arranged by size:
4970 807 143
XmnI GAAnn'nnTTC
Cuts at: 1066 2419 1066
Size: 1353 4567
ZraI GAC'GTC
Cuts at: 669 669
Size: 5920
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI BaeI BbsI BcgI BmtI BsiWI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SspI SwaI XhoI