Restriction map for pF1KSDA0685
 (Linear) (Six-base) MAPSORT of: KIAA0685.seq  Check: 5215  from: 1  to: 2781



KIAA0685.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1978    2781 
   Size:       1978     803
 
AatII G_ACGT'C

Cuts at:      0     545    2781 
   Size:        545    2236
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     775     955    1886    2280    2781 
   Size:        775     180     931     394     501

  Fragments arranged by size: 

                 931     775     501     394     180
 
AfeI AGC'GCT

Cuts at:      0     482     722    2781 
   Size:        482     240    2059

  Fragments arranged by size: 

                2059     482     240
 
AflIII A'CryG_T

Cuts at:      0     761    2324    2781 
   Size:        761    1563     457

  Fragments arranged by size: 

                1563     761     457
 
AleI CACnn'nnGTG

Cuts at:      0    1324    2781 
   Size:       1324    1457
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     644     700    2781 
   Size:        644      56    2081

  Fragments arranged by size: 

                2081     644      56
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      13      45    2781 
   Size:         13      32    2736

  Fragments arranged by size: 

                2736      32      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     753     879     954    1470    2781 
   Size:        129     624     126      75     516    1311

  Fragments arranged by size: 

                1311     624     516     129     126      75
 
ApaI G_GGCC'C

Cuts at:      0    1483    2222    2690    2781 
   Size:       1483     739     468      91

  Fragments arranged by size: 

                1483     739     468      91
 
AvaI C'yCGr_G

Cuts at:      0     600    1862    2005    2587    2781 
   Size:        600    1262     143     582     194

  Fragments arranged by size: 

                1262     600     582     194     143
 
AvrII C'CTAG_G

Cuts at:      0    2682    2781 
   Size:       2682      99
 
BamHI G'GATC_C

Cuts at:      0     189    1234    1364    2781 
   Size:        189    1045     130    1417

  Fragments arranged by size: 

                1417    1045     189     130
 
BanI G'GyrC_C

Cuts at:      0     472    1036    1990    2050    2146    2781 
   Size:        472     564     954      60      96     635

  Fragments arranged by size: 

                 954     635     564     472      96      60
 
BanII G_rGCy'C

Cuts at:      0     533    1013    1094    1483    1520    1893    2222    2383 
   Size:        533     480      81     389      37     373     329     161

Cuts at:   2383    2690    2781 
   Size:        307      91

  Fragments arranged by size: 

                 533     480     389     373     329     307     161      91
                  81      37
 
BbeI G_GCGC'C

Cuts at:      0    1040    2054    2781 
   Size:       1040    1014     727

  Fragments arranged by size: 

                1040    1014     727
 
BbvCI CC'TCA_GC

Cuts at:      0     477    2151    2261    2781 
   Size:        477    1674     110     520

  Fragments arranged by size: 

                1674     520     477     110
 
BfrBI ATG'CAT

Cuts at:      0    1181    2781 
   Size:       1181    1600
 
BglI GCCn_nnn'nGGC

Cuts at:      0     528    1999    2722    2781 
   Size:        528    1471     723      59

  Fragments arranged by size: 

                1471     723     528      59
 
BlpI GC'TnA_GC

Cuts at:      0     307    2781 
   Size:        307    2474
 
Bme1580I G_kGCm'C

Cuts at:      0    1483    1995    2222    2585    2690    2781 
   Size:       1483     512     227     363     105      91

  Fragments arranged by size: 

                1483     512     363     227     105      91
 
BmgBI CAC'GTC

Cuts at:      0      29    1420    2121    2781 
   Size:         29    1391     701     660

  Fragments arranged by size: 

                1391     701     660      29
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1929    2055    2781 
   Size:       1929     126     726

  Fragments arranged by size: 

                1929     726     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718     745    1024    1303    1390    1492    2141    2781 
   Size:        718      27     279     279      87     102     649     640

  Fragments arranged by size: 

                 718     649     640     279     279     102      87      27
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     477    2151    2261    2781 
   Size:        477    1674     110     520

  Fragments arranged by size: 

                1674     520     477     110
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     662     767    2781 
   Size:        662     105    2014

  Fragments arranged by size: 

                2014     662     105
 
BsaHI Gr'CG_yC

Cuts at:      0     542    1037    2051    2781 
   Size:        542     495    1014     730

  Fragments arranged by size: 

                1014     730     542     495
 
BsaWI w'CCGG_w

Cuts at:      0     776    1274    2235    2781 
   Size:        776     498     961     546

  Fragments arranged by size: 

                 961     776     546     498
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2139    2169    2353    2383    2781 
   Size:       2139      30     184      30     398

  Fragments arranged by size: 

                2139     398     184      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     178     223     703    1021    1228    1573    1684    2176 
   Size:        178      45     480     318     207     345     111     492

Cuts at:   2176    2361    2500    2781 
   Size:        185     139     281

  Fragments arranged by size: 

                 492     480     345     318     281     207     185     178
                 139     111      45
 
BseYI C'CCAG_C

Cuts at:      0     660    1075    1292    1541    1759    2171    2568    2646 
   Size:        660     415     217     249     218     412     397      78

Cuts at:   2646    2712    2781 
   Size:         66      69

  Fragments arranged by size: 

                 660     415     412     397     249     218     217      78
                  69      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     532    1045    1378    1483    1507    2247    2645 
   Size:        165     367     513     333     105      24     740     398

Cuts at:   2645    2781 
   Size:        136

  Fragments arranged by size: 

                 740     513     398     367     333     165     136     105
                  24
 
BsiEI CG_ry'CG

Cuts at:      0     282    1301    2448    2495    2781 
   Size:        282    1019    1147      47     286

  Fragments arranged by size: 

                1147    1019     286     282      47
 
BsiHKAI G_wGCw'C

Cuts at:      0    1094    2781 
   Size:       1094    1687
 
BsmI GAATG_Cn'

Cuts at:      0    1026    2254    2781 
   Size:       1026    1228     527

  Fragments arranged by size: 

                1228    1026     527
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1410    1555    1567    2781 
   Size:       1410     145      12    1214

  Fragments arranged by size: 

                1410    1214     145      12
 
Bsp1286I G_dGCh'C

Cuts at:      0     533    1013    1094    1483    1520    1893    1995    2222 
   Size:        533     480      81     389      37     373     102     227

Cuts at:   2222    2383    2585    2690    2781 
   Size:        161     202     105      91

  Fragments arranged by size: 

                 533     480     389     373     227     202     161     105
                 102      91      81      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     500    1978    2781 
   Size:        500    1478     803

  Fragments arranged by size: 

                1478     803     500
 
BsrBI CCG'CTC

Cuts at:      0     711    1476    2781 
   Size:        711     765    1305

  Fragments arranged by size: 

                1305     765     711
 
BsrDI GCAATG_nn'

Cuts at:      0     392    2781 
   Size:        392    2389
 
BsrFI r'CCGG_y

Cuts at:      0    1097    1296    1998    2519    2781 
   Size:       1097     199     702     521     262

  Fragments arranged by size: 

                1097     702     521     262     199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2416    2781 
   Size:       2416     365
 
BstEII G'GTnAC_C

Cuts at:      0    1326    2781 
   Size:       1326    1455
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1458    1644    2318    2781 
   Size:       1458     186     674     463

  Fragments arranged by size: 

                1458     674     463     186
 
BstYI r'GATC_y

Cuts at:      0     189    1234    1364    1619    2781 
   Size:        189    1045     130     255    1162

  Fragments arranged by size: 

                1162    1045     255     189     130
 
BtgI C'CryG_G

Cuts at:      0    1533    2420    2573    2669    2717    2781 
   Size:       1533     887     153      96      48      64

  Fragments arranged by size: 

                1533     887     153      96      64      48
 
BtsI GCAGTG_nn'

Cuts at:      0     160    1054    1794    2072    2781 
   Size:        160     894     740     278     709

  Fragments arranged by size: 

                 894     740     709     278     160
 
EaeI y'GGCC_r

Cuts at:      0    1298    1536    1871    2445    2474    2600    2666    2723 
   Size:       1298     238     335     574      29     126      66      57

Cuts at:   2723    2781 
   Size:         58

  Fragments arranged by size: 

                1298     574     335     238     126      66      58      57
                  29
 
EagI C'GGCC_G

Cuts at:      0    1298    2445    2781 
   Size:       1298    1147     336

  Fragments arranged by size: 

                1298    1147     336
 
EarI CTCTTCn'nnn_

Cuts at:      0     557    2661    2781 
   Size:        557    2104     120

  Fragments arranged by size: 

                2104     557     120
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     182    2781 
   Size:        182    2599
 
EcoICRI GAG'CTC

Cuts at:      0    1092    2781 
   Size:       1092    1689
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718     745     775     955    1024    1303    1390    1492 
   Size:        718      27      30     180      69     279      87     102

Cuts at:   1492    1886    2141    2280    2781 
   Size:        394     255     139     501

  Fragments arranged by size: 

                 718     501     394     279     255     180     139     102
                  87      69      30      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1985    2781 
   Size:       1985     796
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1479    1480    2218    2281    2343    2440    2454    2686 
   Size:       1479       1     738      63      62      97      14     232

Cuts at:   2686    2781 
   Size:         95

  Fragments arranged by size: 

                1479     738     232      97      95      63      62      14
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33     237    1974    2781 
   Size:         33     204    1737     807

  Fragments arranged by size: 

                1737     807     204      33
 
HaeII r_GCGC'y

Cuts at:      0     484     724    1040    2054    2781 
   Size:        484     240     316    1014     727

  Fragments arranged by size: 

                1014     727     484     316     240
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     607     639    2353    2385    2424    2456    2781 
   Size:        607      32    1714      32      39      32     325

  Fragments arranged by size: 

                1714     607     325      39      32      32      32
 
HincII GTy'rAC

Cuts at:      0      39    2781 
   Size:         39    2742
 
KasI G'GCGC_C

Cuts at:      0    1036    2050    2781 
   Size:       1036    1014     731

  Fragments arranged by size: 

                1036    1014     731
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2217    2781 
   Size:       2217     564
 
MscI TGG'CCA

Cuts at:      0    1873    2476    2602    2725    2781 
   Size:       1873     603     126     123      56

  Fragments arranged by size: 

                1873     603     126     123      56
 
MslI CAynn'nnrTG

Cuts at:      0     997    1324    1973    2781 
   Size:        997     327     649     808

  Fragments arranged by size: 

                 997     808     649     327
 
MspA1I CmG'CkG

Cuts at:      0     512     684    1232    1405    1541    2143    2463    2781 
   Size:        512     172     548     173     136     602     320     318

  Fragments arranged by size: 

                 602     548     512     320     318     173     172     136
            
 
NaeI GCC'GGC

Cuts at:      0    1099    1298    2521    2781 
   Size:       1099     199    1223     260

  Fragments arranged by size: 

                1223    1099     260     199
 
NarI GG'CG_CC

Cuts at:      0    1037    2051    2781 
   Size:       1037    1014     730

  Fragments arranged by size: 

                1037    1014     730
 
NcoI C'CATG_G

Cuts at:      0    2420    2717    2781 
   Size:       2420     297      64

  Fragments arranged by size: 

                2420     297      64
 
NgoMIV G'CCGG_C

Cuts at:      0    1097    1296    2519    2781 
   Size:       1097     199    1223     262

  Fragments arranged by size: 

                1223    1097     262     199
 
NsiI A_TGCA'T

Cuts at:      0    1183    2781 
   Size:       1183    1598
 
NspI r_CATG'y

Cuts at:      0      64     765    2781 
   Size:         64     701    2016

  Fragments arranged by size: 

                2016     701      64
 
PasI CC'CwG_GG

Cuts at:      0    1014    2730    2781 
   Size:       1014    1716      51

  Fragments arranged by size: 

                1716    1014      51
 
PciI A'CATG_T

Cuts at:      0     761    2781 
   Size:        761    2020
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1933    2135    2781 
   Size:       1933     202     646

  Fragments arranged by size: 

                1933     646     202
 
PfoI T'CCnGG_A

Cuts at:      0    1367    2781 
   Size:       1367    1414
 
PpuMI rG'GwC_Cy

Cuts at:      0    2440    2781 
   Size:       2440     341
 
PspOMI G'GGCC_C

Cuts at:      0    1479    2218    2686    2781 
   Size:       1479     739     468      95

  Fragments arranged by size: 

                1479     739     468      95
 
PstI C_TGCA'G

Cuts at:      0      74    1789    2781 
   Size:         74    1715     992

  Fragments arranged by size: 

                1715     992      74
 
PvuII CAG'CTG

Cuts at:      0     512     684    2143    2781 
   Size:        512     172    1459     638

  Fragments arranged by size: 

                1459     638     512     172
 
SacI G_AGCT'C

Cuts at:      0    1094    2781 
   Size:       1094    1687
 
SanDI GG'GwC_CC

Cuts at:      0    2440    2781 
   Size:       2440     341
 
SfcI C'TryA_G

Cuts at:      0      70    1785    2781 
   Size:         70    1715     996

  Fragments arranged by size: 

                1715     996      70
 
SfoI GGC'GCC

Cuts at:      0    1038    2052    2781 
   Size:       1038    1014     729

  Fragments arranged by size: 

                1038    1014     729
 
SmaI CCC'GGG

Cuts at:      0    1864    2007    2781 
   Size:       1864     143     774

  Fragments arranged by size: 

                1864     774     143
 
StuI AGG'CCT

Cuts at:      0    1375    1780    2781 
   Size:       1375     405    1001

  Fragments arranged by size: 

                1375    1001     405
 
StyI C'CwwG_G

Cuts at:      0    2277    2420    2682    2717    2781 
   Size:       2277     143     262      35      64

  Fragments arranged by size: 

                2277     262     143      64      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1089    2781 
   Size:       1089    1692
 
TatI w'GTAC_w

Cuts at:      0    1139    2781 
   Size:       1139    1642
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     127     159     174     206    2139    2171    2313    2345 
   Size:        127      32      15      32    1933      32     142      32

Cuts at:   2345    2781 
   Size:        436

  Fragments arranged by size: 

                1933     436     142     127      32      32      32      32
                  15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     999    2392    2781 
   Size:        999    1393     389

  Fragments arranged by size: 

                1393     999     389
 
XmaI C'CCGG_G

Cuts at:      0    1862    2005    2781 
   Size:       1862     143     776

  Fragments arranged by size: 

                1862     776     143
 
XmnI GAAnn'nnTTC

Cuts at:      0     940    2293    2781 
   Size:        940    1353     488

  Fragments arranged by size: 

                1353     940     488
 
ZraI GAC'GTC

Cuts at:      0     543    2781 
   Size:        543    2238
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
    BbvCI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI
   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI     PciI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI
     SfcI     SfoI     SmaI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI    ApaLI     ApoI
     AscI     AseI    AsiSI     BaeI     BbsI     BcgI    BciVI     BclI
    BglII     BmrI     BmtI    BpuEI    BsaAI    BsaBI    BsiWI    BspEI
    BspHI    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     PacI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
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