Restriction map for pF1KSDA0677
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AgeI        
AlwNI       Aor51HI     AscI        AseI        AsnI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      BstBI       BstUI       CelII       
ClaI        CpoI        DraI        DraIII      Eco47III    
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        412  GGTACC               G^GTACC
AflII        1060  CTTAAG               C^TTAAG
AflIII       1748  ACATGT               A^C[GA][CT]GT
AhdI         1490  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1630  GTGCAC               G^TGCAC
ApaI         2348  GGGCCC               GGGCC^C
ApaLI        1630  GTGCAC               G^TGCAC
Asp718I       412  GGTACC               G^GTACC
AspEI        1490  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1784  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          349  CTCGAG               C^[CT]CG[GA]G
BalI         1166  TGGCCA               TGG^CCA
BbvCI        1888  GCTGAGG              CCTCAGC(-5/-2)
BclI          803  TGATCA               T^GATCA
BfrI         1060  CTTAAG               C^TTAAG
BglII         941  AGATCT               A^GATCT
BsaWI        2442  TCCGGT               [AT]^CCGG[AT]
BsiEI        2547  CGGCCG               CG[GA][CT]^CG
BsoBI         349  CTCGAG               C^[CT]CG[GA]G
BspLU11I     1748  ACATGT               A^CATGT
Bst1107I     3007  GTATAC               GTA^TAC
BstEII        820  GGTTACC              G^GT[ACGT]ACC
BstPI         820  GGTTACC              G^GT[ACGT]ACC
BstZ17I      3007  GTATAC               GTA^TAC
EagI         2547  CGGCCG               C^GGCCG
Eam1105I     1490  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2547  CGGCCG               C^GGCCG
Eco52I       2547  CGGCCG               C^GGCCG
EcoO65I       820  GGTTACC              G^GT[ACGT]ACC
FbaI          803  TGATCA               T^GATCA
KpnI          412  GGTACC               GGTAC^C
MaeII        2384  ACGT                 A^CGT
MluNI        1166  TGGCCA               TGG^CCA
MscI         1166  TGGCCA               TGG^CCA
NheI          129  GCTAGC               G^CTAGC
PaeR7I        349  CTCGAG               C^TCGAG
PflFI        1784  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2252  GGGTCCC              [GA]G^G[AT]CC[CT]
ScaI          314  AGTACT               AGT^ACT
SexAI        2957  ACCAGGT              A^CC[AT]GGT
SfiI         1983  GGCCCAACAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         1579  ACTAGT               A^CTAGT
SspI          445  AATATT               AAT^ATT
StuI         1901  AGGCCT               AGG^CCT
Tth111I      1784  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3046  TCTAGA               T^CTAGA
XcmI          209  CCATTCAACAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          349  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          861  GTCTAC               GT^[AC][GT]AC
             3007  GTATAC              
AcyI         1490  GACGCC               G[GA]^CG[CT]C
             1784  GACGCC              
AocI         1876  CCTGAGG              CC^T[ACGT]AGG
             2827  CCTGAGG             
BglI         1984  GCCCAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2674  GCCAAGGGGGC         
BpmI         2854  CTCCAG               CTGGAG(16/14)
              791  CTGGAG              
BsaHI        1490  GACGCC               G[GA]^CG[CT]C
             1784  GACGCC              
BspMI         596  ACCTGC               ACCTGC(4/8)
             2638  ACCTGC              
BsrBI         352  GAGCGG               CCGCTC(-3/-3)
             1717  GAGCGG              
BsrDI          90  CATTGC               GCAATG(2/0)
             2427  CATTGC              
BssSI         164  CACGAG               CACGAG(-5/-1)
             1680  CACGAG              
Bsu36I       1876  CCTGAGG              CC^T[ACGT]AGG
             2827  CCTGAGG             
Eco81I       1876  CCTGAGG              CC^T[ACGT]AGG
             2827  CCTGAGG             
EcoNI        2310  CCTAGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2869  CCTGCTGAAGG         
EcoRI         761  GAATTC               G^AATTC
             1966  GAATTC              
HaeII        1696  AGCGCC               [GA]GCGC^[CT]
             2735  GGCGCT              
Hin1I        1490  GACGCC               G[GA]^CG[CT]C
             1784  GACGCC              
HincII       2422  GTCAAC               GT[CT]^[GA]AC
             2788  GTCAAC              
HindII       2422  GTCAAC               GT[CT]^[GA]AC
             2788  GTCAAC              
NaeI          452  GCCGGC               GCC^GGC
              829  GCCGGC              
NgoMIV        452  GCCGGC               G^CCGGC
              829  GCCGGC              
PflMI         388  CCAATCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1081  CCAAGAGCTGG         
Van91I        388  CCAATCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1081  CCAAGAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI        
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