Restriction map for
pF1KSDA0662
(Circular) (Six-base) MAPSORT of: pF1KSDA0662.seq Check: 5771 from: 1 to: 6442
pF1KSDA0662 6442 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1262 1262
Size: 6442
AatII G_ACGT'C
Cuts at: 362 572 362
Size: 210 6232
AccI GT'mk_AC
Cuts at: 1022 2687 3478 1022
Size: 1665 791 3986
Fragments arranged by size:
3986 1665 791
Acc65I G'GTAC_C
Cuts at: 899 3456 899
Size: 2557 3885
AclI AA'CG_TT
Cuts at: 6282 6282
Size: 6442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 667 1708 1987 2683 4265 4697 5446 5714
Size: 218 1041 279 696 1582 432 749 268
Cuts at: 5714 449
Size: 1177
Fragments arranged by size:
1582 1177 1041 749 696 432 279 268
218
AfeI AGC'GCT
Cuts at: 973 973
Size: 6442
AflIII A'CryG_T
Cuts at: 2162 3792 4898 5875 2162
Size: 1630 1106 977 2729
Fragments arranged by size:
2729 1630 1106 977
AgeI A'CCGG_T
Cuts at: 4830 4830
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1102 1102
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2674 2674
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 3226 3745 5314 3226
Size: 519 1569 4354
Fragments arranged by size:
4354 1569 519
ApaI G_GGCC'C
Cuts at: 3246 3246
Size: 6442
ApaLI G'TGCA_C
Cuts at: 5212 5212
Size: 6442
ApoI r'AATT_y
Cuts at: 1325 1895 3392 3401 3444 5795 6051 1325
Size: 570 1497 9 43 2351 256 1716
Fragments arranged by size:
2351 1716 1497 570 256 43 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 6442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6442
AvaI C'yCGr_G
Cuts at: 218 342 709 1727 3460 218
Size: 124 367 1018 1733 3200
Fragments arranged by size:
3200 1733 1018 367 124
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2888 2921 2888
Size: 33 6409
BamHI G'GATC_C
Cuts at: 3465 3465
Size: 6442
BanI G'GyrC_C
Cuts at: 135 249 886 899 2611 2865 3049 3229 3456
Size: 114 637 13 1712 254 184 180 227
Cuts at: 3456 3870 4118 4153 135
Size: 414 248 35 2424
Fragments arranged by size:
2424 1712 637 414 254 248 227 184
180 114 35 13
BanII G_rGCy'C
Cuts at: 503 1391 1541 2092 2183 2404 2596 3176 3246
Size: 888 150 551 91 221 192 580 70
Cuts at: 3246 3454 4484 503
Size: 208 1030 2461
Fragments arranged by size:
2461 1030 888 580 551 221 208 192
150 91 70
BbeI G_GCGC'C
Cuts at: 253 890 3874 4122 253
Size: 637 2984 248 2573
Fragments arranged by size:
2984 2573 637 248
BbsI GAAGACnn'nnnn_
Cuts at: 437 928 2783 437
Size: 491 1855 4096
Fragments arranged by size:
4096 1855 491
BciVI GTATCCnnnnn_n'
Cuts at: 4338 5101 6442 4338
Size: 763 1341 4338
Fragments arranged by size:
4338 1341 763
BclI T'GATC_A
Cuts at: 3960 3960
Size: 6442
BfrBI ATG'CAT
Cuts at: 802 802
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 1671 1671
Size: 6442
BglII A'GATC_T
Cuts at: 3955 5994 3955
Size: 2039 4403
BlpI GC'TnA_GC
Cuts at: 3552 3552
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 140 1850 2616 3246 4065 4158 5216 140
Size: 1710 766 630 819 93 1058 1366
Fragments arranged by size:
1710 1366 1058 819 766 630 93
BmgBI CAC'GTC
Cuts at: 435 435
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 1204 3652 3733 4068 6214 1204
Size: 2448 81 335 2146 1432
Fragments arranged by size:
2448 2146 1432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1476 1755 3180 4807 1476
Size: 279 1425 1627 3111
Fragments arranged by size:
3111 1627 1425 279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 1600 2476 2785 1279
Size: 321 876 309 4936
Fragments arranged by size:
4936 876 321 309
Bpu10I CC'TnA_GC
Cuts at: 1661 1661
Size: 6442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 2167 3616 4495 4989 5287 895
Size: 1272 1449 879 494 298 2050
Fragments arranged by size:
2050 1449 1272 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2911 6146 2911
Size: 3235 3207
BsaAI yAC'GTr
Cuts at: 582 906 2163 2567 3432 3793 582
Size: 324 1257 404 865 361 3231
Fragments arranged by size:
3231 1257 865 404 361 324
BsaBI GATnn'nnATC
Cuts at: 6 3954 5993 6
Size: 3948 2039 455
Fragments arranged by size:
3948 2039 455
BsaHI Gr'CG_yC
Cuts at: 250 359 569 887 3871 4119 4823 6319 250
Size: 109 210 318 2984 248 704 1496 373
Fragments arranged by size:
2984 1496 704 373 318 248 210 109
BsaWI w'CCGG_w
Cuts at: 1476 3629 4150 4830 5104 5251 5662 1476
Size: 2153 521 680 274 147 411 2256
Fragments arranged by size:
2256 2153 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1118 1148 1528 1558 1573 1603 3001 3031 1118
Size: 30 380 30 15 30 1398 30 4529
Fragments arranged by size:
4529 1398 380 30 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 1549 1993 2047 2313 2455 2974 496
Size: 1053 444 54 266 142 519 3964
Fragments arranged by size:
3964 1053 519 444 266 142 54
BseYI C'CCAG_C
Cuts at: 468 528 2467 2491 2951 3263 3865 4623 5202
Size: 60 1939 24 460 312 602 758 579
Cuts at: 5202 5698 468
Size: 496 1212
Fragments arranged by size:
1939 1212 758 602 579 496 460 312
60 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 2326 2666 391
Size: 1935 340 4167
Fragments arranged by size:
4167 1935 340
BsiEI CG_ry'CG
Cuts at: 104 1348 1477 2370 2803 3371 3982 4028 5238
Size: 1244 129 893 433 568 611 46 1210
Cuts at: 5238 104
Size: 1308
Fragments arranged by size:
1308 1244 1210 893 611 568 433 129
46
BsiHKAI G_wGCw'C
Cuts at: 488 911 1391 1400 2183 2404 2596 3454 4232
Size: 423 480 9 783 221 192 858 778
Cuts at: 4232 4422 5216 488
Size: 190 794 1714
Fragments arranged by size:
1714 858 794 783 778 480 423 221
192 190 9
BsiWI C'GTAC_G
Cuts at: 3349 3349
Size: 6442
BsmI GAATG_Cn'
Cuts at: 1264 1461 2522 1264
Size: 197 1061 5184
Fragments arranged by size:
5184 1061 197
BsmBI CGTCTCn'nnnn_
Cuts at: 1550 3320 1550
Size: 1770 4672
Bsp1286I G_dGCh'C
Cuts at: 140 488 503 911 1391 1400 1541 1850 2092
Size: 348 15 408 480 9 141 309 242
Cuts at: 2092 2183 2404 2596 2616 3176 3246 3454 4065
Size: 91 221 192 20 560 70 208 611
Cuts at: 4065 4158 4232 4422 4484 5216 140
Size: 93 74 190 62 732 1366
Fragments arranged by size:
1366 732 611 560 480 408 348 309
242 221 208 192 190 141 93 91
74 70 62 20 15 9
BspEI T'CCGG_A
Cuts at: 5662 5662
Size: 6442
BspHI T'CATG_A
Cuts at: 5618 5618
Size: 6442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1262 1772 2432 2543 3490 3704 4006 4387 1262
Size: 510 660 111 947 214 302 381 3317
Fragments arranged by size:
3317 947 660 510 381 302 214 111
BsrBI CCG'CTC
Cuts at: 50 4732 4786 6272 6437 50
Size: 4682 54 1486 165 55
Fragments arranged by size:
4682 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 802 2379 4352 802
Size: 1577 1973 2892
Fragments arranged by size:
2892 1973 1577
BsrFI r'CCGG_y
Cuts at: 155 942 3046 3102 4438 4619 4830 155
Size: 787 2104 56 1336 181 211 1767
Fragments arranged by size:
2104 1767 1336 787 211 181 56
BssHII G'CGCG_C
Cuts at: 2450 2450
Size: 6442
BssSI C'ACGA_G
Cuts at: 4711 5071 4711
Size: 360 6082
BstAPI GCAn_nnn'nTGC
Cuts at: 3817 3817
Size: 6442
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 741 741
Size: 6442
BstYI r'GATC_y
Cuts at: 1 1082 1439 3465 3932 3955 4290 4536 5550
Size: 1081 357 2026 467 23 335 246 1014
Cuts at: 5550 5636 5648 5994 1
Size: 86 12 346 449
Fragments arranged by size:
2026 1081 1014 467 449 357 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1074 1074
Size: 6442
BtgI C'CryG_G
Cuts at: 125 294 2525 2607 3269 125
Size: 169 2231 82 662 3298
Fragments arranged by size:
3298 2231 662 169 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4390 4571 4734 4390
Size: 181 163 6098
Fragments arranged by size:
6098 181 163
BtsI GCAGTG_nn'
Cuts at: 270 2640 3683 270
Size: 2370 1043 3029
Fragments arranged by size:
3029 2370 1043
DraI TTT'AAA
Cuts at: 3439 3439
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 4146 5006 4146
Size: 860 5582
EaeI y'GGCC_r
Cuts at: 790 809 950 1430 2367 2528 2577 3134 3252
Size: 19 141 480 937 161 49 557 118
Cuts at: 3252 4025 4199 4590 4617 5964 790
Size: 773 174 391 27 1347 1268
Fragments arranged by size:
1347 1268 937 773 557 480 391 174
161 141 118 49 27 19
EagI C'GGCC_G
Cuts at: 2367 4025 2367
Size: 1658 4784
EarI CTCTTCn'nnn_
Cuts at: 394 1069 1970 2659 2702 4463 4673 394
Size: 675 901 689 43 1761 210 2163
Fragments arranged by size:
2163 1761 901 689 675 210 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3220 4648 4960 5106 6252 3220
Size: 1428 312 146 1146 3410
Fragments arranged by size:
3410 1428 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1389 2181 2402 2594 3452 1389
Size: 792 221 192 858 4379
Fragments arranged by size:
4379 858 792 221 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 667 1279 1600 1708 1987 2476 2683 2785
Size: 218 612 321 108 279 489 207 102
Cuts at: 2785 4265 4697 5446 5714 449
Size: 1480 432 749 268 1177
Fragments arranged by size:
1480 1177 749 612 489 432 321 279
268 218 207 108 102
EcoNI CCTnn'n_nnAGG
Cuts at: 420 1258 2428 420
Size: 838 1170 4434
Fragments arranged by size:
4434 1170 838
EcoO109I rG'GnC_Cy
Cuts at: 690 1673 2078 2105 3242 3579 690
Size: 983 405 27 1137 337 3553
Fragments arranged by size:
3553 1137 983 405 337 27
EcoRI G'AATT_C
Cuts at: 3444 3444
Size: 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 324 4459 324
Size: 4135 2307
FseI GG_CCGG'CC
Cuts at: 946 946
Size: 6442
FspI TGC'GCA
Cuts at: 4221 4221
Size: 6442
HaeII r_GCGC'y
Cuts at: 253 355 890 975 3874 4122 5146 6125 253
Size: 102 535 85 2899 248 1024 979 570
Fragments arranged by size:
2899 1024 979 570 535 248 102 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 915 947 1118 1150 1528 1560 1573 1605 2149
Size: 32 171 32 378 32 13 32 544
Cuts at: 2149 2181 2308 2340 4282 4312 4314 4344 915
Size: 32 127 32 1942 30 2 30 3013
Fragments arranged by size:
3013 1942 544 378 171 127 32 32
32 32 32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 1023 2688 3479 1023
Size: 1665 791 3986
Fragments arranged by size:
3986 1665 791
KasI G'GCGC_C
Cuts at: 249 886 3870 4118 249
Size: 637 2984 248 2573
Fragments arranged by size:
2984 2573 637 248
KpnI G_GTAC'C
Cuts at: 903 3460 903
Size: 2557 3885
MluI A'CGCG_T
Cuts at: 5875 5875
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1497 2069 2147 2228 2570 5113 5297 1497
Size: 572 78 81 342 2543 184 2642
Fragments arranged by size:
2642 2543 572 342 184 81 78
MscI TGG'CCA
Cuts at: 792 811 1432 2530 2579 4201 792
Size: 19 621 1098 49 1622 3033
Fragments arranged by size:
3033 1622 1098 621 49 19
MslI CAynn'nnrTG
Cuts at: 727 1102 2723 4429 4556 727
Size: 375 1621 1706 127 2613
Fragments arranged by size:
2613 1706 1621 375 127
MspA1I CmG'CkG
Cuts at: 231 1296 2410 3226 3334 3551 3865 4225 5240
Size: 1065 1114 816 108 217 314 360 1015
Cuts at: 5240 5485 231
Size: 245 1188
Fragments arranged by size:
1188 1114 1065 1015 816 360 314 245
217 108
NaeI GCC'GGC
Cuts at: 157 944 3048 4621 157
Size: 787 2104 1573 1978
Fragments arranged by size:
2104 1978 1573 787
NarI GG'CG_CC
Cuts at: 250 887 3871 4119 250
Size: 637 2984 248 2573
Fragments arranged by size:
2984 2573 637 248
NcoI C'CATG_G
Cuts at: 125 294 2525 2607 3269 125
Size: 169 2231 82 662 3298
Fragments arranged by size:
3298 2231 662 169 82
NdeI CA'TA_TG
Cuts at: 1170 1170
Size: 6442
NgoMIV G'CCGG_C
Cuts at: 155 942 3046 4619 155
Size: 787 2104 1573 1978
Fragments arranged by size:
2104 1978 1573 787
NruI TCG'CGA
Cuts at: 4803 4803
Size: 6442
NsiI A_TGCA'T
Cuts at: 804 804
Size: 6442
NspI r_CATG'y
Cuts at: 1872 3493 3993 4902 1872
Size: 1621 500 909 3412
Fragments arranged by size:
3412 1621 909 500
PasI CC'CwG_GG
Cuts at: 422 3277 422
Size: 2855 3587
PciI A'CATG_T
Cuts at: 4898 4898
Size: 6442
PflMI CCAn_nnn'nTGG
Cuts at: 3270 3270
Size: 6442
PfoI T'CCnGG_A
Cuts at: 1391 1577 1391
Size: 186 6256
PmeI GTTT'AAAC
Cuts at: 3439 3439
Size: 6442
PmlI CAC'GTG
Cuts at: 906 2567 906
Size: 1661 4781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5581 5613 5581
Size: 32 6410
PpuMI rG'GwC_Cy
Cuts at: 690 690
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 613 613
Size: 6442
PspOMI G'GGCC_C
Cuts at: 3242 3242
Size: 6442
PspXI vC'TCGA_Gb
Cuts at: 709 709
Size: 6442
PstI C_TGCA'G
Cuts at: 2432 2540 2561 2940 3487 4172 2432
Size: 108 21 379 547 685 4702
Fragments arranged by size:
4702 685 547 379 108 21
PvuI CG_AT'CG
Cuts at: 104 1348 104
Size: 1244 5198
PvuII CAG'CTG
Cuts at: 1296 2410 3865 4225 1296
Size: 1114 1455 360 3513
Fragments arranged by size:
3513 1455 1114 360
RsrII CG'GwC_CG
Cuts at: 211 4635 211
Size: 4424 2018
SacI G_AGCT'C
Cuts at: 1391 2183 2404 2596 3454 1391
Size: 792 221 192 858 4379
Fragments arranged by size:
4379 858 792 221 192
SalI G'TCGA_C
Cuts at: 1021 2686 3477 1021
Size: 1665 791 3986
Fragments arranged by size:
3986 1665 791
SapI GCTCTTCn'nnn_
Cuts at: 394 2702 4463 4673 394
Size: 2308 1761 210 2163
Fragments arranged by size:
2308 2163 1761 210
SbfI CC_TGCA'GG
Cuts at: 2540 3487 2540
Size: 947 5495
ScaI AGT'ACT
Cuts at: 832 5959 832
Size: 5127 1315
SexAI A'CCwGG_T
Cuts at: 1016 1016
Size: 6442
SfcI C'TryA_G
Cuts at: 33 669 2428 2536 2557 2835 2936 3483 4168
Size: 636 1759 108 21 278 101 547 685
Cuts at: 4168 5163 5354 33
Size: 995 191 1121
Fragments arranged by size:
1759 1121 995 685 636 547 278 191
108 101 21
SfoI GGC'GCC
Cuts at: 251 888 3872 4120 251
Size: 637 2984 248 2573
Fragments arranged by size:
2984 2573 637 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6442
SmaI CCC'GGG
Cuts at: 220 344 1729 3462 220
Size: 124 1385 1733 3200
Fragments arranged by size:
3200 1733 1385 124
SmlI C'TyrA_G
Cuts at: 709 910 2182 3595 4510 5004 5266 709
Size: 201 1272 1413 915 494 262 1885
Fragments arranged by size:
1885 1413 1272 915 494 262 201
SnaBI TAC'GTA
Cuts at: 3432 3432
Size: 6442
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6442
SphI G_CATG'C
Cuts at: 3493 3993 3493
Size: 500 5942
StuI AGG'CCT
Cuts at: 2542 2883 3148 2542
Size: 341 265 5836
Fragments arranged by size:
5836 341 265
StyI C'CwwG_G
Cuts at: 125 294 1314 1669 1781 2066 2525 2585 2607
Size: 169 1020 355 112 285 459 60 22
Cuts at: 2607 3269 3574 3928 125
Size: 662 305 354 2639
Fragments arranged by size:
2639 1020 662 459 355 354 305 285
169 112 60 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 228 1758 4023 4156 4615 4826 228
Size: 1530 2265 133 459 211 1844
Fragments arranged by size:
2265 1844 1530 459 211 133
TatI w'GTAC_w
Cuts at: 143 830 1937 2459 3215 5957 143
Size: 687 1107 522 756 2742 628
Fragments arranged by size:
2742 1107 756 687 628 522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1733 1765 5959 5991 1733
Size: 32 4194 32 2184
Fragments arranged by size:
4194 2184 32 32
Tth111I GACn'n_nGTC
Cuts at: 1018 4237 1018
Size: 3219 3223
XbaI T'CTAG_A
Cuts at: 3471 3471
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 877 1755 1857 3935 877
Size: 878 102 2078 3384
Fragments arranged by size:
3384 2078 878 102
XhoI C'TCGA_G
Cuts at: 709 709
Size: 6442
XmaI C'CCGG_G
Cuts at: 218 342 1727 3460 218
Size: 124 1385 1733 3200
Fragments arranged by size:
3200 1733 1385 124
XmnI GAAnn'nnTTC
Cuts at: 925 2507 925
Size: 1582 4860
ZraI GAC'GTC
Cuts at: 360 570 360
Size: 210 6232
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BbvCI BcgI BmtI
BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV FspAI
HindIII HpaI MfeI NheI NotI PacI PsiI PsrI
SacII SanDI SfiI SgrAI SrfI SspI SwaI