Restriction map for pF1KSDA0654
 (Linear) (Six-base) MAPSORT of: KIAA0654.seq  Check: 6492  from: 1  to: 3582



KIAA0654.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AccI GT'mk_AC

Cuts at:      0    3018    3582 
   Size:       3018     564
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337    1399    1801    3184    3582 
   Size:        337    1062     402    1383     398

  Fragments arranged by size: 

                1383    1062     402     398     337
 
AfeI AGC'GCT

Cuts at:      0     931    1330    3582 
   Size:        931     399    2252

  Fragments arranged by size: 

                2252     931     399
 
AflIII A'CryG_T

Cuts at:      0    2756    3582 
   Size:       2756     826
 
AleI CACnn'nnGTG

Cuts at:      0    2527    3582 
   Size:       2527    1055
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     247     677    2680    3582 
   Size:        247     430    2003     902

  Fragments arranged by size: 

                2003     902     430     247
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288    1080    1092    1803    1955    2838    3297    3582 
   Size:        288     792      12     711     152     883     459     285

  Fragments arranged by size: 

                 883     792     711     459     288     285     152      12
            
 
ApaI G_GGCC'C

Cuts at:      0      61    1248    2522    3582 
   Size:         61    1187    1274    1060

  Fragments arranged by size: 

                1274    1187    1060      61
 
ApoI r'AATT_y

Cuts at:      0    1179    3169    3307    3582 
   Size:       1179    1990     138     275

  Fragments arranged by size: 

                1990    1179     275     138
 
AvaI C'yCGr_G

Cuts at:      0     426     550    1114    2806    3545    3582 
   Size:        426     124     564    1692     739      37

  Fragments arranged by size: 

                1692     739     564     426     124      37
 
BamHI G'GATC_C

Cuts at:      0    1590    3582 
   Size:       1590    1992
 
BanI G'GyrC_C

Cuts at:      0     811    1560    1579    2037    2062    2245    2602    3582 
   Size:        811     749      19     458      25     183     357     980

  Fragments arranged by size: 

                 980     811     749     458     357     183      25      19
            
 
BanII G_rGCy'C

Cuts at:      0      49      61     188    1248    1547    1658    1693    2106 
   Size:         49      12     127    1060     299     111      35     413

Cuts at:   2106    2522    2975    3483    3490    3500    3582 
   Size:        416     453     508       7      10      82

  Fragments arranged by size: 

                1060     508     453     416     413     299     127     111
                  82      49      35      12      10       7
 
BbvCI CC'TCA_GC

Cuts at:      0    2721    3582 
   Size:       2721     861
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44    2492    2584    2611    3582 
   Size:         44    2448      92      27     971

  Fragments arranged by size: 

                2448     971      92      44      27
 
BlpI GC'TnA_GC

Cuts at:      0     220    1312    3582 
   Size:        220    1092    2270

  Fragments arranged by size: 

                2270    1092     220
 
Bme1580I G_kGCm'C

Cuts at:      0      61     814    1248    1539    1584    2065    2248    2522 
   Size:         61     753     434     291      45     481     183     274

Cuts at:   2522    3210    3582 
   Size:        688     372

  Fragments arranged by size: 

                 753     688     481     434     372     291     274     183
                  61      45
 
BmgBI CAC'GTC

Cuts at:      0     959    1794    3111    3582 
   Size:        959     835    1317     471

  Fragments arranged by size: 

                1317     959     835     471
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     414    1772    3582 
   Size:        414    1358    1810

  Fragments arranged by size: 

                1810    1358     414
 
BmtI G_CTAG'C

Cuts at:      0    1004    2416    3582 
   Size:       1004    1412    1166

  Fragments arranged by size: 

                1412    1166    1004
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     219    1395    1674    2721    3582 
   Size:        219    1176     279    1047     861

  Fragments arranged by size: 

                1176    1047     861     279     219
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     148     310     379     553     733     745     817 
   Size:        103      45     162      69     174     180      12      72

Cuts at:    817     943    1006    1225    1387    1500    1561    1849    1921 
   Size:        126      63     219     162     113      61     288      72

Cuts at:   1921    2120    2494    2560    3091    3319    3582 
   Size:        199     374      66     531     228     263

  Fragments arranged by size: 

                 531     374     288     263     228     219     199     180
                 174     162     162     126     113     103      72      72
                  69      66      63      61      45      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     937    1384    2721    3582 
   Size:        937     447    1337     861

  Fragments arranged by size: 

                1337     937     861     447
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856    1330    2191    2659    2971    3582 
   Size:        856     474     861     468     312     611

  Fragments arranged by size: 

                 861     856     611     474     468     312
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     441    1099    1526    2709    2803    3220    3582 
   Size:        441     658     427    1183      94     417     362

  Fragments arranged by size: 

                1183     658     441     427     417     362      94
 
BsaBI GATnn'nnATC

Cuts at:      0    1477    2650    3582 
   Size:       1477    1173     932

  Fragments arranged by size: 

                1477    1173     932
 
BsaHI Gr'CG_yC

Cuts at:      0     971    1067    3582 
   Size:        971      96    2515

  Fragments arranged by size: 

                2515     971      96
 
BsaWI w'CCGG_w

Cuts at:      0    3059    3566    3582 
   Size:       3059     507      16

  Fragments arranged by size: 

                3059     507      16
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     790     820     943     973     979    1000    1009    1030 
   Size:        790      30     123      30       6      21       9      21

Cuts at:   1030    2767    2797    3307    3337    3582 
   Size:       1737      30     510      30     245

  Fragments arranged by size: 

                1737     790     510     245     123      30      30      30
                  30      21      21       9       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     429     568     571     730     814     817     919    1201 
   Size:        429     139       3     159      84       3     102     282

Cuts at:   1201    1384    1405    1666    1750    1753    1879    1909    2491 
   Size:        183      21     261      84       3     126      30     582

Cuts at:   2491    2724    2788    3582 
   Size:        233      64     794

  Fragments arranged by size: 

                 794     582     429     282     261     233     183     159
                 139     126     102      84      84      64      30      21
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      54     828     944    1211    2008    2825    2887    3156 
   Size:         54     774     116     267     797     817      62     269

Cuts at:   3156    3582 
   Size:        426

  Fragments arranged by size: 

                 817     797     774     426     269     267     116      62
                  54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1816    2245    2650    3582 
   Size:        949     867     429     405     932

  Fragments arranged by size: 

                 949     932     867     429     405
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     458     485     551    1547    2106    2975    3582 
   Size:        188     270      27      66     996     559     869     607

  Fragments arranged by size: 

                 996     869     607     559     270     188      66      27
            
 
BsmI GAATG_Cn'

Cuts at:      0    3282    3582 
   Size:       3282     300
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     124     965    1288    2311    3101    3582 
   Size:        124     841     323    1023     790     481

  Fragments arranged by size: 

                1023     841     790     481     323     124
 
Bsp1286I G_dGCh'C

Cuts at:      0      49      61     188     458     485     551     814    1248 
   Size:         49      12     127     270      27      66     263     434

Cuts at:   1248    1539    1547    1584    1658    1693    2065    2106    2248 
   Size:        291       8      37      74      35     372      41     142

Cuts at:   2248    2522    2975    3210    3483    3490    3500    3582 
   Size:        274     453     235     273       7      10      82

  Fragments arranged by size: 

                 453     434     372     291     274     273     270     263
                 235     142     127      82      74      66      49      41
                  37      35      27      12      10       8       7
 
BspEI T'CCGG_A

Cuts at:      0    3566    3582 
   Size:       3566      16
 
BsrBI CCG'CTC

Cuts at:      0     516     729     909    1191    1464    1674    2159    2600 
   Size:        516     213     180     282     273     210     485     441

Cuts at:   2600    3075    3132    3194    3377    3582 
   Size:        475      57      62     183     205

  Fragments arranged by size: 

                 516     485     475     441     282     273     213     210
                 205     183     180      62      57
 
BsrFI r'CCGG_y

Cuts at:      0      38     695    2121    3582 
   Size:         38     657    1426    1461

  Fragments arranged by size: 

                1461    1426     657      38
 
BssHII G'CGCG_C

Cuts at:      0     735    3582 
   Size:        735    2847
 
BssSI C'ACGA_G

Cuts at:      0     193     379    2108    2770    2860    3582 
   Size:        193     186    1729     662      90     722

  Fragments arranged by size: 

                1729     722     662     193     186      90
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1080    1092    1955    3582 
   Size:       1080      12     863    1627

  Fragments arranged by size: 

                1627    1080     863      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     672    3535    3582 
   Size:        672    2863      47

  Fragments arranged by size: 

                2863     672      47
 
BstYI r'GATC_y

Cuts at:      0    1590    2633    2834    3101    3263    3582 
   Size:       1590    1043     201     267     162     319

  Fragments arranged by size: 

                1590    1043     319     267     201     162
 
Bsu36I CC'TnA_GG

Cuts at:      0    2087    3327    3582 
   Size:       2087    1240     255

  Fragments arranged by size: 

                2087    1240     255
 
BtgI C'CryG_G

Cuts at:      0     401    2230    2525    3582 
   Size:        401    1829     295    1057

  Fragments arranged by size: 

                1829    1057     401     295
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     210    3582 
   Size:        210    3372
 
BtsI GCAGTG_nn'

Cuts at:      0     388     551    1539    1614    2194    3208    3582 
   Size:        388     163     988      75     580    1014     374

  Fragments arranged by size: 

                1014     988     580     388     374     163      75
 
DraIII CAC_nnn'GTG

Cuts at:      0    3012    3582 
   Size:       3012     570
 
EaeI y'GGCC_r

Cuts at:      0      36     189    1049    1451    1805    2016    2123    2393 
   Size:         36     153     860     402     354     211     107     270

Cuts at:   2393    2573    2612    2792    3582 
   Size:        180      39     180     790

  Fragments arranged by size: 

                 860     790     402     354     270     211     180     180
                 153     107      39      36
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     481     623    1025    1030    1214    2275    2364 
   Size:        296     185     142     402       5     184    1061      89

Cuts at:   2364    3582 
   Size:       1218

  Fragments arranged by size: 

                1218    1061     402     296     185     184     142      89
                   5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     337    3405    3582 
   Size:        337    3068     177

  Fragments arranged by size: 

                3068     337     177
 
EcoICRI GAG'CTC

Cuts at:      0     186    1545    2104    2973    3582 
   Size:        186    1359     559     869     609

  Fragments arranged by size: 

                1359     869     609     559     186
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     148     310     337     379     553     733     745 
   Size:        103      45     162      27      42     174     180      12

Cuts at:    745     817     943    1006    1225    1387    1399    1500    1561 
   Size:         72     126      63     219     162      12     101      61

Cuts at:   1561    1801    1849    1921    2120    2494    2560    3091    3184 
   Size:        240      48      72     199     374      66     531      93

Cuts at:   3184    3319    3582 
   Size:        135     263

  Fragments arranged by size: 

                 531     374     263     240     219     199     180     174
                 162     162     135     126     103     101      93      72
                  72      66      63      61      48      45      42      27
                  12      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     874    1387    1515    2476    3582 
   Size:        874     513     128     961    1106

  Fragments arranged by size: 

                1106     961     874     513     128
 
EcoO109I rG'GnC_Cy

Cuts at:      0     492     676     720    2207    2224    2338    2493    3066 
   Size:        492     184      44    1487      17     114     155     573

Cuts at:   3066    3411    3582 
   Size:        345     171

  Fragments arranged by size: 

                1487     573     492     345     184     171     155     114
                  44      17
 
EcoRI G'AATT_C

Cuts at:      0    3307    3582 
   Size:       3307     275
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     492    3582 
   Size:        492    3090
 
FseI GG_CCGG'CC

Cuts at:      0    2125    3582 
   Size:       2125    1457
 
FspI TGC'GCA

Cuts at:      0     394    3582 
   Size:        394    3188
 
HaeII r_GCGC'y

Cuts at:      0      55      90     123     216     414     532     765     883 
   Size:         55      35      33      93     198     118     233     118

Cuts at:    883     933     944    1153    1332    1366    3362    3582 
   Size:         50      11     209     179      34    1996     220

  Fragments arranged by size: 

                1996     233     220     209     198     179     118     118
                  93      55      50      35      34      33      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     943     975    1756    1788    2767    2799    3582 
   Size:        943      32     781      32     979      32     783

  Fragments arranged by size: 

                 979     943     783     781      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1411    3582 
   Size:       1411    2171
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     716    1040    1346    3266    3582 
   Size:        716     324     306    1920     316

  Fragments arranged by size: 

                1920     716     324     316     306
 
MscI TGG'CCA

Cuts at:      0    1807    2018    2395    2614    3582 
   Size:       1807     211     377     219     968

  Fragments arranged by size: 

                1807     968     377     219     211
 
MslI CAynn'nnrTG

Cuts at:      0     400    2527    3582 
   Size:        400    2127    1055

  Fragments arranged by size: 

                2127    1055     400
 
MspA1I CmG'CkG

Cuts at:      0     171     285     603     753     795     891     948    1047 
   Size:        171     114     318     150      42      96      57      99

Cuts at:   1047    1131    1203    1215    1242    1254    1488    1634    1827 
   Size:         84      72      12      27      12     234     146     193

Cuts at:   1827    1955    2664    2825    3439    3475    3582 
   Size:        128     709     161     614      36     107

  Fragments arranged by size: 

                 709     614     318     234     193     171     161     150
                 146     128     114     107      99      96      84      72
                  57      42      36      27      12      12
 
NaeI GCC'GGC

Cuts at:      0      40    2123    3582 
   Size:         40    2083    1459

  Fragments arranged by size: 

                2083    1459      40
 
NdeI CA'TA_TG

Cuts at:      0    2844    3582 
   Size:       2844     738
 
NgoMIV G'CCGG_C

Cuts at:      0      38    2121    3582 
   Size:         38    2083    1461

  Fragments arranged by size: 

                2083    1461      38
 
NheI G'CTAG_C

Cuts at:      0    1000    2412    3582 
   Size:       1000    1412    1170

  Fragments arranged by size: 

                1412    1170    1000
 
NspI r_CATG'y

Cuts at:      0    2559    3582 
   Size:       2559    1023
 
PasI CC'CwG_GG

Cuts at:      0    1711    3483    3582 
   Size:       1711    1772      99

  Fragments arranged by size: 

                1772    1711      99
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1667    1711    2190    2700    3582 
   Size:       1667      44     479     510     882

  Fragments arranged by size: 

                1667     882     510     479      44
 
PfoI T'CCnGG_A

Cuts at:      0     347     509    2471    2693    3401    3582 
   Size:        347     162    1962     222     708     181

  Fragments arranged by size: 

                1962     708     347     222     181     162
 
PpuMI rG'GwC_Cy

Cuts at:      0     676    2207    2224    2338    3066    3411    3582 
   Size:        676    1531      17     114     728     345     171

  Fragments arranged by size: 

                1531     728     676     345     171     114      17
 
PspOMI G'GGCC_C

Cuts at:      0      57    1244    2518    3582 
   Size:         57    1187    1274    1064

  Fragments arranged by size: 

                1274    1187    1064      57
 
PspXI vC'TCGA_Gb

Cuts at:      0     550    3582 
   Size:        550    3032
 
PstI C_TGCA'G

Cuts at:      0     212     863    1085    1097    1241    2201    3263    3474 
   Size:        212     651     222      12     144     960    1062     211

Cuts at:   3474    3518    3582 
   Size:         44      64

  Fragments arranged by size: 

                1062     960     651     222     212     211     144      64
                  44      12
 
PvuII CAG'CTG

Cuts at:      0     171     285     603     753     795    1047    1131    1203 
   Size:        171     114     318     150      42     252      84      72

Cuts at:   1203    1215    1488    1827    1955    2825    3439    3582 
   Size:         12     273     339     128     870     614     143

  Fragments arranged by size: 

                 870     614     339     318     273     252     171     150
                 143     128     114      84      72      42      12
 
RsrII CG'GwC_CG

Cuts at:      0    3564    3582 
   Size:       3564      18
 
SacI G_AGCT'C

Cuts at:      0     188    1547    2106    2975    3582 
   Size:        188    1359     559     869     607

  Fragments arranged by size: 

                1359     869     607     559     188
 
SanDI GG'GwC_CC

Cuts at:      0    2207    2224    2338    3582 
   Size:       2207      17     114    1244

  Fragments arranged by size: 

                2207    1244     114      17
 
SapI GCTCTTCn'nnn_

Cuts at:      0     623    1030    2275    2364    3582 
   Size:        623     407    1245      89    1218

  Fragments arranged by size: 

                1245    1218     623     407      89
 
SfcI C'TryA_G

Cuts at:      0     161     208     859    1081    1093    1237    2197    3259 
   Size:        161      47     651     222      12     144     960    1062

Cuts at:   3259    3470    3514    3582 
   Size:        211      44      68

  Fragments arranged by size: 

                1062     960     651     222     211     161     144      68
                  47      44      12
 
SmaI CCC'GGG

Cuts at:      0     428    3547    3582 
   Size:        428    3119      35

  Fragments arranged by size: 

                3119     428      35
 
SmlI C'TyrA_G

Cuts at:      0     550     871    1345    2170    2674    2986    3582 
   Size:        550     321     474     825     504     312     596

  Fragments arranged by size: 

                 825     596     550     504     474     321     312
 
SphI G_CATG'C

Cuts at:      0    2559    3582 
   Size:       2559    1023
 
StuI AGG'CCT

Cuts at:      0    2188    2482    3582 
   Size:       2188     294    1100

  Fragments arranged by size: 

                2188    1100     294
 
StyI C'CwwG_G

Cuts at:      0    1661    2189    2813    3582 
   Size:       1661     528     624     769

  Fragments arranged by size: 

                1661     769     624     528
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1578    2163    2459    2538    2856    3135    3552    3582 
   Size:       1578     585     296      79     318     279     417      30

  Fragments arranged by size: 

                1578     585     417     318     296     279      79      30
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1691    1723    3582 
   Size:       1691      32    1859

  Fragments arranged by size: 

                1859    1691      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     427     981    1923    2403    2697    3582 
   Size:        427     554     942     480     294     885

  Fragments arranged by size: 

                 942     885     554     480     427     294
 
XhoI C'TCGA_G

Cuts at:      0     550    3582 
   Size:        550    3032
 
XmaI C'CCGG_G

Cuts at:      0     426    3545    3582 
   Size:        426    3119      37

  Fragments arranged by size: 

                3119     426      37
 
XmnI GAAnn'nnTTC

Cuts at:      0    3307    3397    3582 
   Size:       3307      90     185

  Fragments arranged by size: 

                3307     185      90
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII    BbvCI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    HaeII    Hin4I  HindIII     MmeI
     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV     NheI     NspI
     PasI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII
    RsrII     SacI    SanDI     SapI     SfcI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII    BsaAI    BsiEI    BsiWI
    BspHI    BspMI    BsrDI    BsrGI    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI    EcoRV    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NcoI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI     PvuI    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
     TatI  Tth111I     XbaI     ZraI
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