Restriction map for pF1KSDA0652
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BcnI        BglI        BglII       BlnI        
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstYI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        HpaII       
KasI        Ksp632I     KspI        MaeII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspI        MunI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TaqI        
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          588  GTCTAC               GT^[AC][GT]AC
AccII        1519  CGCG                 CG^CG
AflII         256  CTTAAG               C^TTAAG
AlwNI        1250  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI        1451  GAGCAC               G[AT]GC[AT]^C
BbvCI        1420  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1521  CGAGTTTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          636  TGATCA               T^GATCA
BfaI          395  CTAG                 C^TAG
BfrI          256  CTTAAG               C^TTAAG
BlpI         1425  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1425  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1216  GATACCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1099  GAGACC               GGTCTC(1/5)
BsiHKAI      1451  GAGCAC               G[AT]GC[AT]^C
BsmI          580  GCATTC               GAATGC(1/-1)
Bsp1407I      350  TGTACA               T^GTACA
BspMI         988  ACCTGC               ACCTGC(4/8)
BsrDI         365  CATTGC               GCAATG(2/0)
BsrGI         350  TGTACA               T^GTACA
BstEII       1152  GGTGACC              G^GT[ACGT]ACC
BstPI        1152  GGTGACC              G^GT[ACGT]ACC
BstUI        1519  CGCG                 CG^CG
CelII        1425  GCTGAGC              GC^T[ACGT]AGC
DraI         1345  TTTAAA               TTT^AAA
DraIII        640  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1152  GGTGACC              G^GT[ACGT]ACC
FauI         1298  GCGGG                CCCGC(4/6)
FbaI          636  TGATCA               T^GATCA
HaeIII       1077  GGCC                 GG^CC
HincII       1132  GTCAAC               GT[CT]^[GA]AC
HindII       1132  GTCAAC               GT[CT]^[GA]AC
MaeI          395  CTAG                 C^TAG
MamI         1216  GATACCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1519  CGCG                 CG^CG
PflMI         897  CCAGCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI        1148  ACCAGGT              A^CC[AT]GGT
SfaNI        1528  GATGC                GCATC(5/9)
SspBI         350  TGTACA               T^GTACA
Van91I        897  CCAGCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Acc65I        927  GGTACC               G^GTACC
              958  GGTACC              
AciI         1518  CCGC                 CCGC(-3/-1)
             1298  GCGG                
AcsI           52  AAATTT               [GA]^AATT[CT]
             1200  GAATTT              
AlwI         1222  GATCC                GGATC(4/5)
              835  GGATC               
ApoI           52  AAATTT               [GA]^AATT[CT]
             1200  GAATTT              
Asp718I       927  GGTACC               G^GTACC
              958  GGTACC              
BbsI          736  GAAGAC               GAAGAC(2/6)
             1468  GAAGAC              
BciVI        1041  GGATAC               GTATCC(6/5)
             1215  GGATAC              
BpuAI         736  GAAGAC               GAAGAC(2/6)
             1468  GAAGAC              
BseRI         666  CTCCTC               GAGGAG(10/8)
             1262  CTCCTC              
BsgI          990  CTGCAC               GTGCAG(16/14)
             1279  CTGCAC              
BstF5I        171  CATCC                GGATG(2/0)
              782  CATCC               
FokI          171  CATCC                GGATG(9/13)
              782  CATCC               
KpnI          927  GGTACC               GGTAC^C
              958  GGTACC              
PleI          739  GACTC                GAGTC(4/5)
             1477  GACTC               
PstI          221  CTGCAG               CTGCA^G
             1546  CTGCAG              
Tsp45I        400  GTGAC                ^GT[GC]AC
             1153  GTGAC               
XcmI           87  CCAGGCTCGGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              945  CCAGCCTGTCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AfaI        AluI        AvaII       BanI        BbvI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       BstXI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HinfI       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       
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