Restriction map for pF1KSDA0652
 (Linear) (Six-base) MAPSORT of: KIAA0652.seq  Check: 7095  from: 1  to: 1551



KIAA0652.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AccI GT'mk_AC

Cuts at:      0     589    1551 
   Size:        589     962
 
Acc65I G'GTAC_C

Cuts at:      0     927     958    1551 
   Size:        927      31     593

  Fragments arranged by size: 

                 927     593      31
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    1487    1551 
   Size:        394    1093      64

  Fragments arranged by size: 

                1093     394      64
 
AflII C'TTAA_G

Cuts at:      0     256    1551 
   Size:        256    1295
 
AleI CACnn'nnGTG

Cuts at:      0     535    1551 
   Size:        535    1016
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1255    1551 
   Size:       1255     296
 
ApoI r'AATT_y

Cuts at:      0      52    1200    1551 
   Size:         52    1148     351

  Fragments arranged by size: 

                1148     351      52
 
BanI G'GyrC_C

Cuts at:      0     529     541     927     958    1551 
   Size:        529      12     386      31     593

  Fragments arranged by size: 

                 593     529     386      31      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     743    1475    1551 
   Size:        743     732      76

  Fragments arranged by size: 

                 743     732      76
 
BbvCI CC'TCA_GC

Cuts at:      0    1421    1551 
   Size:       1421     130
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1510    1544    1551 
   Size:       1510      34       7

  Fragments arranged by size: 

                1510      34       7
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1035    1209    1551 
   Size:       1035     174     342

  Fragments arranged by size: 

                1035     342     174
 
BclI T'GATC_A

Cuts at:      0     636    1551 
   Size:        636     915
 
BlpI GC'TnA_GC

Cuts at:      0    1426    1551 
   Size:       1426     125
 
Bme1580I G_kGCm'C

Cuts at:      0     532     544    1551 
   Size:        532      12    1007

  Fragments arranged by size: 

                1007     532      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     734    1014    1223    1231    1252    1278    1551 
   Size:        405     329     280     209       8      21      26     273

  Fragments arranged by size: 

                 405     329     280     273     209      26      21       8
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     489    1421    1551 
   Size:        489     932     130

  Fragments arranged by size: 

                 932     489     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     728     777    1551 
   Size:         49     679      49     774

  Fragments arranged by size: 

                 774     679      49      49
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1093    1551 
   Size:       1093     458
 
BsaBI GATnn'nnATC

Cuts at:      0    1220    1551 
   Size:       1220     331
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1245    1275    1551 
   Size:       1245      30     276

  Fragments arranged by size: 

                1245     276      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     657    1253    1551 
   Size:        657     596     298

  Fragments arranged by size: 

                 657     596     298
 
BseYI C'CCAG_C

Cuts at:      0     661     785    1551 
   Size:        661     124     766

  Fragments arranged by size: 

                 766     661     124
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     975    1264    1551 
   Size:        975     289     287

  Fragments arranged by size: 

                 975     289     287
 
BsiHKAI G_wGCw'C

Cuts at:      0    1455    1551 
   Size:       1455      96
 
BsmI GAATG_Cn'

Cuts at:      0     580    1551 
   Size:        580     971
 
Bsp1286I G_dGCh'C

Cuts at:      0     532     544    1455    1551 
   Size:        532      12     911      96

  Fragments arranged by size: 

                 911     532      96      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     997    1551 
   Size:        997     554
 
BsrDI GCAATG_nn'

Cuts at:      0     364    1551 
   Size:        364    1187
 
BsrGI T'GTAC_A

Cuts at:      0     350    1551 
   Size:        350    1201
 
BstEII G'GTnAC_C

Cuts at:      0    1152    1551 
   Size:       1152     399
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     243     285    1203    1551 
   Size:        243      42     918     348

  Fragments arranged by size: 

                 918     348     243      42
 
BtgI C'CryG_G

Cuts at:      0     278     954    1460    1551 
   Size:        278     676     506      91

  Fragments arranged by size: 

                 676     506     278      91
 
BtsI GCAGTG_nn'

Cuts at:      0    1071    1314    1551 
   Size:       1071     243     237

  Fragments arranged by size: 

                1071     243     237
 
DraI TTT'AAA

Cuts at:      0    1347    1551 
   Size:       1347     204
 
DraIII CAC_nnn'GTG

Cuts at:      0     645    1551 
   Size:        645     906
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     405     734    1014    1223    1231    1252    1278 
   Size:        394      11     329     280     209       8      21      26

Cuts at:   1278    1487    1551 
   Size:        209      64

  Fragments arranged by size: 

                 394     329     280     209     209      64      26      21
                  11       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     608    1391    1551 
   Size:         33     575     783     160

  Fragments arranged by size: 

                 783     575     160      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      99     131    1243    1245    1275    1277    1383    1415 
   Size:         99      32    1112       2      30       2     106      32

Cuts at:   1415    1462    1494    1551 
   Size:         47      32      57

  Fragments arranged by size: 

                1112     106      99      57      47      32      32      32
                  30       2       2
 
HincII GTy'rAC

Cuts at:      0    1134    1551 
   Size:       1134     417
 
KpnI G_GTAC'C

Cuts at:      0     931     962    1551 
   Size:        931      31     589

  Fragments arranged by size: 

                 931     589      31
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     813    1551 
   Size:        157     656     738

  Fragments arranged by size: 

                 738     656     157
 
MslI CAynn'nnrTG

Cuts at:      0     241     440     535    1286    1551 
   Size:        241     199      95     751     265

  Fragments arranged by size: 

                 751     265     241     199      95
 
MspA1I CmG'CkG

Cuts at:      0     216     480     841     900    1298    1425    1551 
   Size:        216     264     361      59     398     127     126

  Fragments arranged by size: 

                 398     361     264     216     127     126      59
 
NcoI C'CATG_G

Cuts at:      0     278     954    1460    1551 
   Size:        278     676     506      91

  Fragments arranged by size: 

                 676     506     278      91
 
PasI CC'CwG_GG

Cuts at:      0     825    1551 
   Size:        825     726
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     903    1551 
   Size:        903     648
 
PfoI T'CCnGG_A

Cuts at:      0     421    1551 
   Size:        421    1130
 
PpuMI rG'GwC_Cy

Cuts at:      0      33     608    1391    1551 
   Size:         33     575     783     160

  Fragments arranged by size: 

                 783     575     160      33
 
PstI C_TGCA'G

Cuts at:      0     225    1550    1551 
   Size:        225    1325       1

  Fragments arranged by size: 

                1325     225       1
 
PvuII CAG'CTG

Cuts at:      0     216     480     841     900    1425    1551 
   Size:        216     264     361      59     525     126

  Fragments arranged by size: 

                 525     361     264     216     126      59
 
SexAI A'CCwGG_T

Cuts at:      0    1148    1551 
   Size:       1148     403
 
SfcI C'TryA_G

Cuts at:      0     221     411     731    1546    1551 
   Size:        221     190     320     815       5

  Fragments arranged by size: 

                 815     320     221     190       5
 
SmlI C'TyrA_G

Cuts at:      0      64     256     743     792    1551 
   Size:         64     192     487      49     759

  Fragments arranged by size: 

                 759     487     192      64      49
 
StyI C'CwwG_G

Cuts at:      0     278     954    1460    1551 
   Size:        278     676     506      91

  Fragments arranged by size: 

                 676     506     278      91
 
TatI w'GTAC_w

Cuts at:      0     350    1551 
   Size:        350    1201
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     280     312     906     938    1551 
   Size:        280      32     594      32     613

  Fragments arranged by size: 

                 613     594     280      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      94     952    1551 
   Size:         94     858     599

  Fragments arranged by size: 

                 858     599      94
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII     AleI    AlwNI     ApoI     BanI
     BbsI    BbvCI     BcgI    BciVI     BclI     BlpI Bme1580I     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrGI   BstEII    BstXI
     BtgI     BtsI     DraI   DraIII  Eco57MI EcoO109I    Hin4I   HincII
     KpnI     MmeI     MslI   MspA1I     NcoI     PasI    PflMI     PfoI
    PpuMI     PstI    PvuII    SexAI     SfcI     SmlI     StyI     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI   AflIII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI    BfrBI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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