Restriction map for
pF1KSDA0645
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BbeI BcgI BsaBI BsiWI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 1876 TCCGGA T^CCGGA
AclI 2547 AACGTT AA^CGTT
AcyI 1378 GACGCC G[GA]^CG[CT]C
AflII 172 CTTAAG C^TTAAG
BanI 3274 GGCACC G^G[CT][GA]CC
BbrPI 3556 CACGTG CAC^GTG
BfrI 172 CTTAAG C^TTAAG
BglII 1444 AGATCT A^GATCT
BsaAI 3556 CACGTG [CT]AC^GT[GA]
BsaHI 1378 GACGCC G[GA]^CG[CT]C
BsaI 237 GAGACC GGTCTC(1/5)
BsaWI 1876 TCCGGA [AT]^CCGG[AT]
BseAI 1876 TCCGGA T^CCGGA
BsiEI 3705 CGACCG CG[GA][CT]^CG
Bsp1407I 4353 TGTACA T^GTACA
BspEI 1876 TCCGGA T^CCGGA
BsrBI 4531 GAGCGG CCGCTC(-3/-3)
BsrDI 4332 CATTGC GCAATG(2/0)
BsrGI 4353 TGTACA T^GTACA
BssSI 3814 CTCGTG CACGAG(-5/-1)
BstAPI 3600 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4083 GGTGACC G^GT[ACGT]ACC
BstPI 4083 GGTGACC G^GT[ACGT]ACC
DraIII 2554 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4317 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4083 GGTGACC G^GT[ACGT]ACC
EcoRI 2692 GAATTC G^AATTC
Hin1I 1378 GACGCC G[GA]^CG[CT]C
MroI 1876 TCCGGA T^CCGGA
NdeI 3288 CATATG CA^TATG
PaeR7I 1661 CTCGAG C^TCGAG
PmaCI 3556 CACGTG CAC^GTG
PmlI 3556 CACGTG CAC^GTG
Psp1406I 2547 AACGTT AA^CGTT
ScaI 4095 AGTACT AGT^ACT
SexAI 1761 ACCTGGT A^CC[AT]GGT
SphI 4701 GCATGC GCATG^C
SspBI 4353 TGTACA T^GTACA
SspI 4364 AATATT AAT^ATT
StuI 2487 AGGCCT AGG^CCT
XhoI 1661 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 50 enzymes) #
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AccI 16 GTCTAC GT^[AC][GT]AC
268 GTAGAC
AccII 2591 CGCG CG^CG
3528 CGCG
AcsI 697 GAATTT [GA]^AATT[CT]
2692 GAATTC
AflIII 370 ACATGT A^C[GA][CT]GT
4517 ACATGT
AhdI 2032 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACTGGAAGTC
Alw44I 1709 GTGCAC G^TGCAC
3817 GTGCAC
AocI 274 CCTAAGG CC^T[ACGT]AGG
2509 CCTGAGG
ApaI 1421 GGGCCC GGGCC^C
3028 GGGCCC
ApaLI 1709 GTGCAC G^TGCAC
3817 GTGCAC
ApoI 697 GAATTT [GA]^AATT[CT]
2692 GAATTC
AspEI 2032 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACTGGAAGTC
AvrII 1575 CCTAGG C^CTAGG
2113 CCTAGG
BalI 3584 TGGCCA TGG^CCA
3713 TGGCCA
BamHI 1947 GGATCC G^GATCC
3566 GGATCC
BbsI 754 GAAGAC GAAGAC(2/6)
2764 GAAGAC
BbvCI 899 CCTCAGC CCTCAGC(-5/-2)
3086 CCTCAGC
BclI 1004 TGATCA T^GATCA
2579 TGATCA
BglI 3796 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3855 GCCTAGCCGGC
BlnI 1575 CCTAGG C^CTAGG
2113 CCTAGG
BlpI 2085 GCTTAGC GC^T[ACGT]AGC
2460 GCTGAGC
Bpu1102I 2085 GCTTAGC GC^T[ACGT]AGC
2460 GCTGAGC
BpuAI 754 GAAGAC GAAGAC(2/6)
2764 GAAGAC
BsmBI 2389 CGTCTC CGTCTC(1/5)
2676 CGTCTC
BspLU11I 370 ACATGT A^CATGT
4517 ACATGT
BstUI 2591 CGCG CG^CG
3528 CGCG
BstXI 3212 CCACCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3324 CCAGACCTTTGG
Bsu36I 274 CCTAAGG CC^T[ACGT]AGG
2509 CCTGAGG
CelII 2085 GCTTAGC GC^T[ACGT]AGC
2460 GCTGAGC
Eam1105I 2032 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACTGGAAGTC
Eco81I 274 CCTAAGG CC^T[ACGT]AGG
2509 CCTGAGG
EcoT22I 3632 ATGCAT ATGCA^T
4699 ATGCAT
FbaI 1004 TGATCA T^GATCA
2579 TGATCA
HincII 3054 GTCAAC GT[CT]^[GA]AC
3979 GTTGAC
HindII 3054 GTCAAC GT[CT]^[GA]AC
3979 GTTGAC
HindIII 100 AAGCTT A^AGCTT
1825 AAGCTT
MluNI 3584 TGGCCA TGG^CCA
3713 TGGCCA
MscI 3584 TGGCCA TGG^CCA
3713 TGGCCA
MvnI 2591 CGCG CG^CG
3528 CGCG
NaeI 2755 GCCGGC GCC^GGC
3860 GCCGGC
NcoI 3305 CCATGG C^CATGG
4029 CCATGG
NgoMIV 2755 GCCGGC G^CCGGC
3860 GCCGGC
NsiI 3632 ATGCAT ATGCA^T
4699 ATGCAT
PpuMI 2116 AGGACCC [GA]G^G[AT]CC[CT]
2800 AGGACCC
PshAI 648 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3471 GACAGGAGTC
SacI 3168 GAGCTC GAGCT^C
3811 GAGCTC
SmaI 1012 CCCGGG CCC^GGG
1418 CCCGGG
XbaI 664 TCTAGA T^CTAGA
2136 TCTAGA
XcmI 3038 CCACGCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3257 CCACTCCTATGTTGG
XmaCI 1012 CCCGGG C^CCGGG
1418 CCCGGG
XmaI 1012 CCCGGG C^CCGGG
1418 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BanII
BbvI BciVI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI