Restriction map for pF1KSDA0644
 (Linear) (Six-base) MAPSORT of: KIAA0644.seq  Check: 8924  from: 1  to: 2433



KIAA0644.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     761    1335    2433 
   Size:        761     574    1098

  Fragments arranged by size: 

                1098     761     574
 
AccI GT'mk_AC

Cuts at:      0    1910    2078    2433 
   Size:       1910     168     355

  Fragments arranged by size: 

                1910     355     168
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1129    2433 
   Size:       1129    1304
 
AfeI AGC'GCT

Cuts at:      0      88    1114    1440    2433 
   Size:         88    1026     326     993

  Fragments arranged by size: 

                1026     993     326      88
 
AflIII A'CryG_T

Cuts at:      0    2219    2433 
   Size:       2219     214
 
AleI CACnn'nnGTG

Cuts at:      0    2260    2308    2433 
   Size:       2260      48     125

  Fragments arranged by size: 

                2260     125      48
 
AlwNI CAG_nnn'CTG

Cuts at:      0     753    1224    2091    2433 
   Size:        753     471     867     342

  Fragments arranged by size: 

                 867     753     471     342
 
ApaI G_GGCC'C

Cuts at:      0    1465    1510    2202    2433 
   Size:       1465      45     692     231

  Fragments arranged by size: 

                1465     692     231      45
 
ApaLI G'TGCA_C

Cuts at:      0     120    2433 
   Size:        120    2313
 
ApoI r'AATT_y

Cuts at:      0    2341    2433 
   Size:       2341      92
 
AvaI C'yCGr_G

Cuts at:      0     369     730    1464    1562    2021    2277    2433 
   Size:        369     361     734      98     459     256     156

  Fragments arranged by size: 

                 734     459     369     361     256     156      98
 
BamHI G'GATC_C

Cuts at:      0     569    1234    1245    2433 
   Size:        569     665      11    1188

  Fragments arranged by size: 

                1188     665     569      11
 
BanI G'GyrC_C

Cuts at:      0    2254    2433 
   Size:       2254     179
 
BanII G_rGCy'C

Cuts at:      0      74     137     172     989    1465    1510    1589    2202 
   Size:         74      63      35     817     476      45      79     613

Cuts at:   2202    2433 
   Size:        231

  Fragments arranged by size: 

                 817     613     476     231      79      74      63      45
                  35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     513    1149    2433 
   Size:        513     636    1284

  Fragments arranged by size: 

                1284     636     513
 
BbvCI CC'TCA_GC

Cuts at:      0    1257    2433 
   Size:       1257    1176
 
BglI GCCn_nnn'nGGC

Cuts at:      0     200     502     971    2072    2143    2433 
   Size:        200     302     469    1101      71     290

  Fragments arranged by size: 

                1101     469     302     290     200      71
 
BlpI GC'TnA_GC

Cuts at:      0     865     988    1009    2433 
   Size:        865     123      21    1424

  Fragments arranged by size: 

                1424     865     123      21
 
Bme1580I G_kGCm'C

Cuts at:      0      82     124     149     376    1465    1510    1669    2202 
   Size:         82      42      25     227    1089      45     159     533

Cuts at:   2202    2257    2433 
   Size:         55     176

  Fragments arranged by size: 

                1089     533     227     176     159      82      55      45
                  42      25
 
BmgBI CAC'GTC

Cuts at:      0     180     760    2433 
   Size:        180     580    1673

  Fragments arranged by size: 

                1673     580     180
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1686    2433 
   Size:       1686     747
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     333    2433 
   Size:        333    2100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     577     871     910     928    1351    1456    2433 
   Size:        481      96     294      39      18     423     105     977

  Fragments arranged by size: 

                 977     481     423     294     105      96      39      18
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     436     778     922    1257    2433 
   Size:        436     342     144     335    1176

  Fragments arranged by size: 

                1176     436     342     335     144
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1339    2433 
   Size:       1339    1094
 
BsaHI Gr'CG_yC

Cuts at:      0    1321    2075    2152    2433 
   Size:       1321     754      77     281

  Fragments arranged by size: 

                1321     754     281      77
 
BsaWI w'CCGG_w

Cuts at:      0     566    2052    2433 
   Size:        566    1486     381

  Fragments arranged by size: 

                1486     566     381
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1587    1617    2209    2239    2433 
   Size:       1587      30     592      30     194

  Fragments arranged by size: 

                1587     592     194      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     684    1138    1327    1357    2433 
   Size:        684     454     189      30    1076

  Fragments arranged by size: 

                1076     684     454     189      30
 
BseYI C'CCAG_C

Cuts at:      0     107     599    1044    1390    1480    1544    1808    1842 
   Size:        107     492     445     346      90      64     264      34

Cuts at:   1842    2433 
   Size:        591

  Fragments arranged by size: 

                 591     492     445     346     264     107      90      64
                  34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     883     931    1183    1516    2433 
   Size:        277     606      48     252     333     917

  Fragments arranged by size: 

                 917     606     333     277     252      48
 
BsiEI CG_ry'CG

Cuts at:      0    1304    1840    2355    2433 
   Size:       1304     536     515      78

  Fragments arranged by size: 

                1304     536     515      78
 
BsiHKAI G_wGCw'C

Cuts at:      0     124     185     989    1676    1826    2132    2433 
   Size:        124      61     804     687     150     306     301

  Fragments arranged by size: 

                 804     687     306     301     150     124      61
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     766    2159    2391    2416    2433 
   Size:        766    1393     232      25      17

  Fragments arranged by size: 

                1393     766     232      25      17
 
Bsp1286I G_dGCh'C

Cuts at:      0      74      82     124     137     149     172     185     376 
   Size:         74       8      42      13      12      23      13     191

Cuts at:    376     989    1465    1510    1589    1669    1676    1826    2132 
   Size:        613     476      45      79      80       7     150     306

Cuts at:   2132    2202    2257    2433 
   Size:         70      55     176

  Fragments arranged by size: 

                 613     476     306     191     176     150      80      79
                  74      70      55      45      42      23      13      13
                  12       8       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     275     548     761    1093    1321    1335    1538    1922 
   Size:        275     273     213     332     228      14     203     384

Cuts at:   1922    2433 
   Size:        511

  Fragments arranged by size: 

                 511     384     332     275     273     228     213     203
                  14
 
BsrBI CCG'CTC

Cuts at:      0     287     926    2433 
   Size:        287     639    1507

  Fragments arranged by size: 

                1507     639     287
 
BsrDI GCAATG_nn'

Cuts at:      0     936    2433 
   Size:        936    1497
 
BsrFI r'CCGG_y

Cuts at:      0     974    1094    1142    1627    2257    2294    2433 
   Size:        974     120      48     485     630      37     139

  Fragments arranged by size: 

                 974     630     485     139     120      48      37
 
BssHII G'CGCG_C

Cuts at:      0    1644    1850    1852    2315    2433 
   Size:       1644     206       2     463     118

  Fragments arranged by size: 

                1644     463     206     118       2
 
BssSI C'ACGA_G

Cuts at:      0      31    2433 
   Size:         31    2402
 
BstEII G'GTnAC_C

Cuts at:      0    2043    2433 
   Size:       2043     390
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1422    2433 
   Size:       1422    1011
 
BstYI r'GATC_y

Cuts at:      0     281     569    1062    1234    1245    1569    2433 
   Size:        281     288     493     172      11     324     864

  Fragments arranged by size: 

                 864     493     324     288     281     172      11
 
Bsu36I CC'TnA_GG

Cuts at:      0     809    2433 
   Size:        809    1624
 
BtgI C'CryG_G

Cuts at:      0     441    1298    1502    2249    2433 
   Size:        441     857     204     747     184

  Fragments arranged by size: 

                 857     747     441     204     184
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1604    2433 
   Size:       1604     829
 
BtsI GCAGTG_nn'

Cuts at:      0    1171    2028    2433 
   Size:       1171     857     405

  Fragments arranged by size: 

                1171     857     405
 
DraIII CAC_nnn'GTG

Cuts at:      0     180    2312    2433 
   Size:        180    2132     121

  Fragments arranged by size: 

                2132     180     121
 
EaeI y'GGCC_r

Cuts at:      0      65     665     710     953     963    1301    1837    1881 
   Size:         65     600      45     243      10     338     536      44

Cuts at:   1881    1934    2296    2433 
   Size:         53     362     137

  Fragments arranged by size: 

                 600     536     362     338     243     137      65      53
                  45      44      10
 
EagI C'GGCC_G

Cuts at:      0    1301    1837    2433 
   Size:       1301     536     596

  Fragments arranged by size: 

                1301     596     536
 
EarI CTCTTCn'nnn_

Cuts at:      0     323    2433 
   Size:        323    2110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     930    1849    2202    2256    2344    2433 
   Size:        930     919     353      54      88      89

  Fragments arranged by size: 

                 930     919     353      89      88      54
 
EcoICRI GAG'CTC

Cuts at:      0     987    2433 
   Size:        987    1446
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     577     871     910     928    1129    1351    1456 
   Size:        481      96     294      39      18     201     222     105

Cuts at:   1456    2433 
   Size:        977

  Fragments arranged by size: 

                 977     481     294     222     201     105      96      39
                  18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     355    2053    2433 
   Size:        355    1698     380

  Fragments arranged by size: 

                1698     380     355
 
EcoO109I rG'GnC_Cy

Cuts at:      0     456     831    1461    2198    2199    2433 
   Size:        456     375     630     737       1     234

  Fragments arranged by size: 

                 737     630     456     375     234       1
 
EcoRI G'AATT_C

Cuts at:      0    2341    2433 
   Size:       2341      92
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     609     633    2433 
   Size:        609      24    1800

  Fragments arranged by size: 

                1800     609      24
 
FspI TGC'GCA

Cuts at:      0     391     400     997    2433 
   Size:        391       9     597    1436

  Fragments arranged by size: 

                1436     597     391       9
 
HaeII r_GCGC'y

Cuts at:      0      90     364     508     916    1016    1116    1442    1799 
   Size:         90     274     144     408     100     100     326     357

Cuts at:   1799    2433 
   Size:        634

  Fragments arranged by size: 

                 634     408     357     326     274     144     100     100
                  90
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     273     305    1117    1149    1641    1673    1783    1815 
   Size:        273      32     812      32     492      32     110      32

Cuts at:   1815    2433 
   Size:        618

  Fragments arranged by size: 

                 812     618     492     273     110      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2079    2115    2433 
   Size:       2079      36     318

  Fragments arranged by size: 

                2079     318      36
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     587    1582    1721    1754    2433 
   Size:        587     995     139      33     679

  Fragments arranged by size: 

                 995     679     587     139      33
 
MscI TGG'CCA

Cuts at:      0    1883    2433 
   Size:       1883     550
 
MslI CAynn'nnrTG

Cuts at:      0    2260    2308    2433 
   Size:       2260      48     125

  Fragments arranged by size: 

                2260     125      48
 
MspA1I CmG'CkG

Cuts at:      0      63     387     443     681     873    1224    1273    1504 
   Size:         63     324      56     238     192     351      49     231

Cuts at:   1504    1548    1722    1842    2088    2433 
   Size:         44     174     120     246     345

  Fragments arranged by size: 

                 351     345     324     246     238     231     192     174
                 120      63      56      49      44
 
NaeI GCC'GGC

Cuts at:      0     976    1096    1144    1629    2433 
   Size:        976     120      48     485     804

  Fragments arranged by size: 

                 976     804     485     120      48
 
NcoI C'CATG_G

Cuts at:      0    2249    2433 
   Size:       2249     184
 
NgoMIV G'CCGG_C

Cuts at:      0     974    1094    1142    1627    2433 
   Size:        974     120      48     485     806

  Fragments arranged by size: 

                 974     806     485     120      48
 
NspI r_CATG'y

Cuts at:      0    2223    2433 
   Size:       2223     210
 
PasI CC'CwG_GG

Cuts at:      0     498    2433 
   Size:        498    1935
 
PciI A'CATG_T

Cuts at:      0    2219    2433 
   Size:       2219     214
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243    2433 
   Size:        243    2190
 
PspOMI G'GGCC_C

Cuts at:      0    1461    1506    2198    2433 
   Size:       1461      45     692     235

  Fragments arranged by size: 

                1461     692     235      45
 
PstI C_TGCA'G

Cuts at:      0     272     725    1332    2420    2433 
   Size:        272     453     607    1088      13

  Fragments arranged by size: 

                1088     607     453     272      13
 
PvuII CAG'CTG

Cuts at:      0     873    1224    2088    2433 
   Size:        873     351     864     345

  Fragments arranged by size: 

                 873     864     351     345
 
SacI G_AGCT'C

Cuts at:      0     989    2433 
   Size:        989    1444
 
SacII CC_GC'GG

Cuts at:      0     444    1505    2433 
   Size:        444    1061     928

  Fragments arranged by size: 

                1061     928     444
 
SalI G'TCGA_C

Cuts at:      0    2077    2433 
   Size:       2077     356
 
SapI GCTCTTCn'nnn_

Cuts at:      0     323    2433 
   Size:        323    2110
 
SbfI CC_TGCA'GG

Cuts at:      0    1332    2433 
   Size:       1332    1101
 
SexAI A'CCwGG_T

Cuts at:      0    1970    2433 
   Size:       1970     463
 
SfcI C'TryA_G

Cuts at:      0     189     268     643     721    1328    2416    2433 
   Size:        189      79     375      78     607    1088      17

  Fragments arranged by size: 

                1088     607     375     189      79      78      17
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     971    2143    2433 
   Size:        971    1172     290

  Fragments arranged by size: 

                1172     971     290
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1627    2257    2433 
   Size:       1627     630     176

  Fragments arranged by size: 

                1627     630     176
 
SmaI CCC'GGG

Cuts at:      0     371    1466    2023    2433 
   Size:        371    1095     557     410

  Fragments arranged by size: 

                1095     557     410     371
 
SrfI GCCC'GGGC

Cuts at:      0    1466    2433 
   Size:       1466     967
 
StuI AGG'CCT

Cuts at:      0     814    1106    2433 
   Size:        814     292    1327

  Fragments arranged by size: 

                1327     814     292
 
StyI C'CwwG_G

Cuts at:      0     530    2096    2138    2249    2433 
   Size:        530    1566      42     111     184

  Fragments arranged by size: 

                1566     530     184     111      42
 
TatI w'GTAC_w

Cuts at:      0     272    2222    2433 
   Size:        272    1950     211

  Fragments arranged by size: 

                1950     272     211
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2209    2241    2433 
   Size:       2209      32     192

  Fragments arranged by size: 

                2209     192      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     517    2433 
   Size:        517    1916
 
XbaI T'CTAG_A

Cuts at:      0    1065    2433 
   Size:       1065    1368
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1699    2433 
   Size:       1699     734
 
XmaI C'CCGG_G

Cuts at:      0     369    1464    2021    2433 
   Size:        369    1095     557     412

  Fragments arranged by size: 

                1095     557     412     369
 
XmnI GAAnn'nnTTC

Cuts at:      0     592    2433 
   Size:        592    1841
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI   PspOMI     PstI    PvuII     SacI    SacII     SalI
     SapI     SbfI    SexAI     SfcI     SfiI    SgrAI     SmaI     SrfI
     StuI     StyI     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII     BmtI    BpuEI    BsaAI    BsaBI    BsiWI
     BsmI    BspEI    BspHI    BsrGI   BstAPI    BstBI  BstZ17I     ClaI
    CspCI     DraI     DrdI    EcoRV     FseI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SanDI     ScaI
     SfoI     SgfI     SmlI    SnaBI     SpeI     SphI     SspI     SwaI
    TaqII     XhoI     ZraI
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