Restriction map for pF1KSDA0639
##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       Acc65I      AgeI        Alw44I      ApaLI       
AscI        Asp718I     AviII       AvrII       BlnI        
BsaBI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      BstAPI      BstBI       
ClaI        CpoI        DrdI        FseI        FspI        
KpnI        MamI        MluI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshAI       PvuI        RcaI        RsrII       
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
StuI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AclI         2031  AACGTT               AA^CGTT
AflII        2001  CTTAAG               C^TTAAG
AflIII       2923  ACACGT               A^C[GA][CT]GT
Aor51HI      1987  AGCGCT               AGC^GCT
AseI          363  ATTAAT               AT^TAAT
AsnI          363  ATTAAT               AT^TAAT
Asp700I       466  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1674  GGATCC               G^GATCC
BfrI         2001  CTTAAG               C^TTAAG
BssSI        4879  CTCGTG               CACGAG(-5/-1)
Bst1107I     3149  GTATAC               GTA^TAC
BstZ17I      3149  GTATAC               GTA^TAC
Eco47III     1987  AGCGCT               AGC^GCT
EcoRV        1438  GATATC               GAT^ATC
HindIII      3334  AAGCTT               A^AGCTT
KspI         3686  CCGCGG               CCGC^GG
NaeI         5031  GCCGGC               GCC^GGC
NgoMIV       5031  GCCGGC               G^CCGGC
PshBI         363  ATTAAT               AT^TAAT
Psp1406I     2031  AACGTT               AA^CGTT
SacI         5200  GAGCTC               GAGCT^C
SacII        3686  CCGCGG               CCGC^GG
SalI          547  GTCGAC               G^TCGAC
SphI         4982  GCATGC               GCATG^C
SspI         3919  AATATT               AAT^ATT
XmnI          466  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AspHI        1900  GAGCAC               G[AT]GC[AT]^C
             5200  GAGCTC              
AspI         4728  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5083  GACGAGGTC           
BalI         2405  TGGCCA               TGG^CCA
             5423  TGGCCA              
BbrPI        2500  CACGTG               CAC^GTG
             5184  CACGTG              
BciVI          32  GGATAC               GTATCC(6/5)
             4240  GTATCC              
BclI          174  TGATCA               T^GATCA
             2772  TGATCA              
BglII        4179  AGATCT               A^GATCT
             4580  AGATCT              
BsiHKAI      1900  GAGCAC               G[AT]GC[AT]^C
             5200  GAGCTC              
BsmBI        4439  CGTCTC               CGTCTC(1/5)
             4716  CGTCTC              
BsrBI        3187  CCGCTC               CCGCTC(-3/-3)
             3733  GAGCGG              
DraI          430  TTTAAA               TTT^AAA
             1398  TTTAAA              
DraIII       1066  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2656  CACTTTGTG           
EcoRI        1460  GAATTC               G^AATTC
             2968  GAATTC              
EcoT22I       287  ATGCAT               ATGCA^T
             3290  ATGCAT              
FbaI          174  TGATCA               T^GATCA
             2772  TGATCA              
MfeI         2843  CAATTG               C^AATTG
             3325  CAATTG              
MluNI        2405  TGGCCA               TGG^CCA
             5423  TGGCCA              
MscI         2405  TGGCCA               TGG^CCA
             5423  TGGCCA              
MunI         2843  CAATTG               C^AATTG
             3325  CAATTG              
NcoI          974  CCATGG               C^CATGG
             1581  CCATGG              
NsiI          287  ATGCAT               ATGCA^T
             3290  ATGCAT              
PaeR7I       2419  CTCGAG               C^TCGAG
             4909  CTCGAG              
PflFI        4728  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5083  GACGAGGTC           
PmaCI        2500  CACGTG               CAC^GTG
             5184  CACGTG              
PmlI         2500  CACGTG               CAC^GTG
             5184  CACGTG              
ScaI         3311  AGTACT               AGT^ACT
             5249  AGTACT              
Tth111I      4728  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5083  GACGAGGTC           
XhoI         2419  CTCGAG               C^TCGAG
             4909  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  151 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AhdI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspEI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspEI       BspMI       
BsrDI       BsrFI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaI        HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MroI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        
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