Restriction map for
pF1KSDA0639
(Circular) (Six-base) MAPSORT of: pF1KSDA0639.seq Check: 3031 from: 1 to: 8617
pF1KSDA0639 8617 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 674 1844 3276 5653 674
Size: 1170 1432 2377 3638
Fragments arranged by size:
3638 2377 1432 1170
Acc65I G'GTAC_C
Cuts at: 5631 5631
Size: 8617
AclI AA'CG_TT
Cuts at: 2158 8457 2158
Size: 6299 2318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1417 1728 2861 5364 6440 6872 7621 7889
Size: 462 311 1133 2503 1076 432 749 268
Cuts at: 7889 955
Size: 1683
Fragments arranged by size:
2503 1683 1133 1076 749 462 432 311
268
AfeI AGC'GCT
Cuts at: 2115 2115
Size: 8617
AflII C'TTAA_G
Cuts at: 2127 2127
Size: 8617
AflIII A'CryG_T
Cuts at: 3049 5967 7073 8050 3049
Size: 2918 1106 977 3616
Fragments arranged by size:
3616 2918 1106 977
AgeI A'CCGG_T
Cuts at: 7005 7005
Size: 8617
AhdI GACnn_n'nnGTC
Cuts at: 670 1617 3053 670
Size: 947 1436 6234
Fragments arranged by size:
6234 1436 947
AleI CACnn'nnGTG
Cuts at: 3850 3850
Size: 8617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4618 4618
Size: 8617
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4251 4283 5575 5607 4251
Size: 32 1292 32 7261
Fragments arranged by size:
7261 1292 32 32
AlwNI CAG_nnn'CTG
Cuts at: 757 919 2775 2807 3516 3616 3703 4123 5184
Size: 162 1856 32 709 100 87 420 1061
Cuts at: 5184 5920 7489 757
Size: 736 1569 1885
Fragments arranged by size:
1885 1856 1569 1061 736 709 420 162
100 87 32
ApaI G_GGCC'C
Cuts at: 3594 4666 4695 4796 4876 5443 3594
Size: 1072 29 101 80 567 6768
Fragments arranged by size:
6768 1072 567 101 80 29
ApaLI G'TGCA_C
Cuts at: 7387 7387
Size: 8617
ApoI r'AATT_y
Cuts at: 581 1027 1554 1586 3094 5619 7970 8226 581
Size: 446 527 32 1508 2525 2351 256 972
Fragments arranged by size:
2525 2351 1508 972 527 446 256 32
AseI AT'TA_AT
Cuts at: 20 490 20
Size: 470 8147
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8617
AvaI C'yCGr_G
Cuts at: 272 1078 2545 4933 5035 5136 5163 5482 5508
Size: 806 1467 2388 102 101 27 319 26
Cuts at: 5508 5635 272
Size: 127 3254
Fragments arranged by size:
3254 2388 1467 806 319 127 102 101
27 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3596 3629 3596
Size: 33 8584
BamHI G'GATC_C
Cuts at: 1800 5640 1800
Size: 3840 4777
BanI G'GyrC_C
Cuts at: 4938 5046 5167 5254 5476 5631 6045 6293 6328
Size: 108 121 87 222 155 414 248 35
Cuts at: 6328 4938
Size: 7227
Fragments arranged by size:
7227 414 248 222 155 121 108 87
35
BanII G_rGCy'C
Cuts at: 3324 3594 4666 4695 4796 4876 4934 4988 5014
Size: 270 1072 29 101 80 58 54 26
Cuts at: 5014 5330 5443 5515 5629 6659 3324
Size: 316 113 72 114 1030 5282
Fragments arranged by size:
5282 1072 1030 316 270 114 113 101
80 72 58 54 29 26
BbeI G_GCGC'C
Cuts at: 4942 5050 5171 5258 6049 6297 4942
Size: 108 121 87 791 248 7262
Fragments arranged by size:
7262 791 248 121 108 87
BbsI GAAGACnn'nnnn_
Cuts at: 916 1430 2532 2939 3049 3365 916
Size: 514 1102 407 110 316 6168
Fragments arranged by size:
6168 1102 514 407 316 110
BbvCI CC'TCA_GC
Cuts at: 2141 2182 2237 3830 2141
Size: 41 55 1593 6928
Fragments arranged by size:
6928 1593 55 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4702 4736 5134 5168 5298 5332 4702
Size: 34 398 34 130 34 7987
Fragments arranged by size:
7987 398 130 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 152 4377 6513 7276 8617 152
Size: 4225 2136 763 1341 152
Fragments arranged by size:
4225 2136 1341 763 152
BclI T'GATC_A
Cuts at: 300 2898 6135 300
Size: 2598 3237 2782
Fragments arranged by size:
3237 2782 2598
BfrBI ATG'CAT
Cuts at: 415 3418 415
Size: 3003 5614
BglI GCCn_nnn'nGGC
Cuts at: 1105 4924 5485 1105
Size: 3819 561 4237
Fragments arranged by size:
4237 3819 561
BglII A'GATC_T
Cuts at: 4305 4706 6130 8169 4305
Size: 401 1424 2039 4753
Fragments arranged by size:
4753 2039 1424 401
BlpI GC'TnA_GC
Cuts at: 2117 3835 3895 4649 5727 2117
Size: 1718 60 754 1078 5007
Fragments arranged by size:
5007 1718 1078 754 60
Bme1580I G_kGCm'C
Cuts at: 472 3594 3847 4592 4666 4695 4796 4876 5311
Size: 3122 253 745 74 29 101 80 435
Cuts at: 5311 5443 5481 6240 6333 7391 472
Size: 132 38 759 93 1058 1698
Fragments arranged by size:
3122 1698 1058 759 745 435 253 132
101 93 80 74 38 29
BmgBI CAC'GTC
Cuts at: 1619 4671 1619
Size: 3052 5565
BmrI ACTGGGnnnn_n'
Cuts at: 2452 2768 3604 3737 5827 5908 6243 8389 2452
Size: 316 836 133 2090 81 335 2146 2680
Fragments arranged by size:
2680 2146 2090 836 335 316 133 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5112 6982 5112
Size: 1870 6747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 1280 1671 1842 2023 2274 3538 4424 872
Size: 408 391 171 181 251 1264 886 5065
Fragments arranged by size:
5065 1264 886 408 391 251 181 171
Bpu10I CC'TnA_GC
Cuts at: 2141 2182 2237 3830 2141
Size: 41 55 1593 6928
Fragments arranged by size:
6928 1593 55 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 4504 5791 6670 7164 7462 1432
Size: 3072 1287 879 494 298 2587
Fragments arranged by size:
3072 2587 1287 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 596 2411 2700 2704 3331 8321 596
Size: 1815 289 4 627 4990 892
Fragments arranged by size:
4990 1815 892 627 289 4
BsaAI yAC'GTr
Cuts at: 2628 3052 5283 5312 5607 5968 2628
Size: 424 2231 29 295 361 5277
Fragments arranged by size:
5277 2231 424 361 295 29
BsaBI GATnn'nnATC
Cuts at: 6 6129 8168 6
Size: 6123 2039 455
Fragments arranged by size:
6123 2039 455
BsaHI Gr'CG_yC
Cuts at: 4865 4939 5047 5168 5255 6046 6294 6998 8494
Size: 74 108 121 87 791 248 704 1496
Cuts at: 8494 4865
Size: 4988
Fragments arranged by size:
4988 1496 791 704 248 121 108 87
74
BsaWI w'CCGG_w
Cuts at: 2045 4776 5069 5585 5804 6325 7005 7279 7426
Size: 2731 293 516 219 521 680 274 147
Cuts at: 7426 7837 2045
Size: 411 2825
Fragments arranged by size:
2825 2731 680 521 516 411 293 274
219 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 845 875 1837 1867 2839 2869 3511 3541 3962
Size: 30 962 30 972 30 642 30 421
Cuts at: 3962 3992 4251 4281 4758 4788 4828 4858 845
Size: 30 259 30 477 30 40 30 4604
Fragments arranged by size:
4604 972 962 642 477 421 259 40
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 790 1375 1396 1634 1845 2158 2254 2844 2847
Size: 585 21 238 211 313 96 590 3
Cuts at: 2847 3556 3757 4272 4507 4657 4996 790
Size: 709 201 515 235 150 339 4411
Fragments arranged by size:
4411 709 590 585 515 339 313 238
235 211 201 150 96 21 3
BseYI C'CCAG_C
Cuts at: 1494 2110 3594 4116 4798 4997 5116 5416 5472
Size: 616 1484 522 682 199 119 300 56
Cuts at: 5472 6040 6798 7377 7873 1494
Size: 568 758 579 496 2238
Fragments arranged by size:
2238 1484 758 682 616 579 568 522
496 300 199 119 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1982 2608 3259 4624 4699 1982
Size: 626 651 1365 75 5900
Fragments arranged by size:
5900 1365 651 626 75
BsiEI CG_ry'CG
Cuts at: 104 3957 4564 4970 5019 6157 6203 7413 104
Size: 3853 607 406 49 1138 46 1210 1308
Fragments arranged by size:
3853 1308 1210 1138 607 406 49 46
BsiHKAI G_wGCw'C
Cuts at: 2030 5330 5629 6407 6597 7391 2030
Size: 3300 299 778 190 794 3256
Fragments arranged by size:
3300 3256 794 778 299 190
BsmI GAATG_Cn'
Cuts at: 399 1491 5157 399
Size: 1092 3666 3859
Fragments arranged by size:
3859 3666 1092
BsmBI CGTCTCn'nnnn_
Cuts at: 4571 4848 4571
Size: 277 8340
Bsp1286I G_dGCh'C
Cuts at: 472 2030 3324 3594 3847 4592 4666 4695 4796
Size: 1558 1294 270 253 745 74 29 101
Cuts at: 4796 4876 4934 4988 5014 5311 5330 5443 5481
Size: 80 58 54 26 297 19 113 38
Cuts at: 5481 5515 5629 6240 6333 6407 6597 6659 7391
Size: 34 114 611 93 74 190 62 732
Cuts at: 7391 472
Size: 1698
Fragments arranged by size:
1698 1558 1294 745 732 611 297 270
253 190 114 113 101 93 80 74
74 62 58 54 38 34 29 26
19
BspEI T'CCGG_A
Cuts at: 2045 4776 5069 7837 2045
Size: 2731 293 2768 2825
Fragments arranged by size:
2825 2768 2731 293
BspHI T'CATG_A
Cuts at: 7793 7793
Size: 8617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 2605 4728 5342 5665 5879 6181 6562 337
Size: 2268 2123 614 323 214 302 381 2392
Fragments arranged by size:
2392 2268 2123 614 381 323 302 214
BsrBI CCG'CTC
Cuts at: 50 3315 3861 6907 6961 8447 8612 50
Size: 3265 546 3046 54 1486 165 55
Fragments arranged by size:
3265 3046 1486 546 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1292 1941 2231 2901 2913 6527 1292
Size: 649 290 670 12 3614 3382
Fragments arranged by size:
3614 3382 670 649 290 12
BsrFI r'CCGG_y
Cuts at: 173 1310 2823 4343 5157 6613 6794 7005 173
Size: 1137 1513 1520 814 1456 181 211 1785
Fragments arranged by size:
1785 1520 1513 1456 1137 814 211 181
BssSI C'ACGA_G
Cuts at: 5005 6886 7246 5005
Size: 1881 360 6376
Fragments arranged by size:
6376 1881 360
BstAPI GCAn_nnn'nTGC
Cuts at: 5992 5992
Size: 8617
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8617
BstEII G'GTnAC_C
Cuts at: 1911 4629 4764 1911
Size: 2718 135 5764
Fragments arranged by size:
5764 2718 135
BstXI CCAn_nnnn'nTGG
Cuts at: 204 2211 3090 3602 5526 204
Size: 2007 879 512 1924 3295
Fragments arranged by size:
3295 2007 1924 879 512
BstYI r'GATC_y
Cuts at: 1 1233 1800 4305 4443 4706 5640 6107 6130
Size: 1232 567 2505 138 263 934 467 23
Cuts at: 6130 6465 6711 7725 7811 7823 8169 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
2505 1232 1014 934 567 467 449 346
335 263 246 138 86 23 12
BstZ17I GTA'TAC
Cuts at: 3277 3277
Size: 8617
Bsu36I CC'TnA_GG
Cuts at: 4156 4922 5340 4156
Size: 766 418 7433
Fragments arranged by size:
7433 766 418
BtgI C'CryG_G
Cuts at: 125 1100 1707 3812 3851 4964 5249 5435 125
Size: 975 607 2105 39 1113 285 186 3307
Fragments arranged by size:
3307 2105 1113 975 607 285 186 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2693 4556 6565 6746 6909 2693
Size: 1863 2009 181 163 4401
Fragments arranged by size:
4401 2009 1863 181 163
BtsI GCAGTG_nn'
Cuts at: 513 900 2880 3582 4223 5858 513
Size: 387 1980 702 641 1635 3272
Fragments arranged by size:
3272 1980 1635 702 641 387
DraI TTT'AAA
Cuts at: 558 1526 5614 558
Size: 968 4088 3561
Fragments arranged by size:
4088 3561 968
DraIII CAC_nnn'GTG
Cuts at: 1197 2787 1197
Size: 1590 7027
DrdI GACnn_nn'nnGTC
Cuts at: 6321 7181 6321
Size: 860 7757
EaeI y'GGCC_r
Cuts at: 2531 3954 4561 4967 5549 6200 6374 6765 6792
Size: 1423 607 406 582 651 174 391 27
Cuts at: 6792 8139 2531
Size: 1347 3009
Fragments arranged by size:
3009 1423 1347 651 607 582 406 391
174 27
EagI C'GGCC_G
Cuts at: 3954 4561 4967 6200 3954
Size: 607 406 1233 6371
Fragments arranged by size:
6371 1233 607 406
EarI CTCTTCn'nnn_
Cuts at: 1436 2327 2865 3347 3734 4543 6638 6848 1436
Size: 891 538 482 387 809 2095 210 3205
Fragments arranged by size:
3205 2095 891 809 538 482 387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5505 6823 7135 7281 8427 5505
Size: 1318 312 146 1146 5695
Fragments arranged by size:
5695 1318 1146 312 146
EcoICRI GAG'CTC
Cuts at: 5328 5627 5328
Size: 299 8318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 955 1280 1417 1671 1728 1842 2023 2274
Size: 83 325 137 254 57 114 181 251
Cuts at: 2274 2861 3538 4424 5364 6440 6872 7621 7889
Size: 587 677 886 940 1076 432 749 268
Cuts at: 7889 872
Size: 1600
Fragments arranged by size:
1600 1076 940 886 749 677 587 432
325 268 254 251 181 137 114 83
57
EcoNI CCTnn'n_nnAGG
Cuts at: 2425 3538 5551 2425
Size: 1113 2013 5491
Fragments arranged by size:
5491 2013 1113
EcoO109I rG'GnC_Cy
Cuts at: 664 1209 1923 3590 3591 4663 4691 4692 4793
Size: 545 714 1667 1 1072 28 1 101
Cuts at: 4793 4873 5040 5121 5181 5214 5287 5440 5754
Size: 80 167 81 60 33 73 153 314
Cuts at: 5754 664
Size: 3527
Fragments arranged by size:
3527 1667 1072 714 545 314 167 153
101 81 80 73 60 33 28 1
1
EcoRI G'AATT_C
Cuts at: 1586 3094 5619 1586
Size: 1508 2525 4584
Fragments arranged by size:
4584 2525 1508
EcoRV GAT'ATC
Cuts at: 1566 1566
Size: 8617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3529 4545 5394 6634 3529
Size: 1016 849 1240 5512
Fragments arranged by size:
5512 1240 1016 849
FspI TGC'GCA
Cuts at: 6396 6396
Size: 8617
HaeII r_GCGC'y
Cuts at: 920 2010 2117 4942 5028 5050 5171 5258 5501
Size: 1090 107 2825 86 22 121 87 243
Cuts at: 5501 6049 6297 7321 8300 920
Size: 548 248 1024 979 1237
Fragments arranged by size:
2825 1237 1090 1024 979 548 248 243
121 107 87 86 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 368 400 656 688 703 735 823 855 1603
Size: 32 256 32 15 32 88 32 748
Cuts at: 1603 1635 1837 1869 2820 2839 2852 2871 3039
Size: 32 202 32 951 19 13 19 168
Cuts at: 3039 3071 3206 3238 3658 3690 3981 4013 4179
Size: 32 135 32 420 32 291 32 166
Cuts at: 4179 4211 4292 4324 5001 5033 5323 5355 6457
Size: 32 81 32 677 32 290 32 1102
Cuts at: 6457 6487 6489 6519 368
Size: 30 2 30 2466
Fragments arranged by size:
2466 1102 951 748 677 420 291 290
256 202 168 166 135 88 81 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 19
19 15 13 2
HincII GTy'rAC
Cuts at: 675 1881 2922 3530 4232 5654 675
Size: 1206 1041 608 702 1422 3638
Fragments arranged by size:
3638 1422 1206 1041 702 608
HindIII A'AGCT_T
Cuts at: 3460 3460
Size: 8617
HpaI GTT'AAC
Cuts at: 1881 2922 4232 1881
Size: 1041 1310 6266
Fragments arranged by size:
6266 1310 1041
KasI G'GCGC_C
Cuts at: 4938 5046 5167 5254 6045 6293 4938
Size: 108 121 87 791 248 7262
Fragments arranged by size:
7262 791 248 121 108 87
KpnI G_GTAC'C
Cuts at: 5635 5635
Size: 8617
MfeI C'AATT_G
Cuts at: 2969 3451 2969
Size: 482 8135
MluI A'CGCG_T
Cuts at: 8050 8050
Size: 8617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1593 2387 4841 7288 7472 1593
Size: 794 2454 2447 184 2738
Fragments arranged by size:
2738 2454 2447 794 184
MscI TGG'CCA
Cuts at: 2533 5551 6376 2533
Size: 3018 825 4774
Fragments arranged by size:
4774 3018 825
MslI CAynn'nnrTG
Cuts at: 2527 2906 3850 4600 4627 6604 6731 2527
Size: 379 944 750 27 1977 127 4413
Fragments arranged by size:
4413 1977 944 750 379 127 27
MspA1I CmG'CkG
Cuts at: 475 1126 1269 1836 3814 4762 5542 5726 6040
Size: 651 143 567 1978 948 780 184 314
Cuts at: 6040 6400 7415 7660 475
Size: 360 1015 245 1432
Fragments arranged by size:
1978 1432 1015 948 780 651 567 360
314 245 184 143
NaeI GCC'GGC
Cuts at: 5159 6796 5159
Size: 1637 6980
NarI GG'CG_CC
Cuts at: 4939 5047 5168 5255 6046 6294 4939
Size: 108 121 87 791 248 7262
Fragments arranged by size:
7262 791 248 121 108 87
NcoI C'CATG_G
Cuts at: 125 1100 1707 125
Size: 975 607 7035
Fragments arranged by size:
7035 975 607
NgoMIV G'CCGG_C
Cuts at: 5157 6794 5157
Size: 1637 6980
NruI TCG'CGA
Cuts at: 6978 6978
Size: 8617
NsiI A_TGCA'T
Cuts at: 417 3420 417
Size: 3003 5614
NspI r_CATG'y
Cuts at: 419 3915 5112 5668 6168 7077 419
Size: 3496 1197 556 500 909 1959
Fragments arranged by size:
3496 1959 1197 909 556 500
PasI CC'CwG_GG
Cuts at: 1926 4062 4869 1926
Size: 2136 807 5674
Fragments arranged by size:
5674 2136 807
PciI A'CATG_T
Cuts at: 7073 7073
Size: 8617
PflMI CCAn_nnn'nTGG
Cuts at: 1197 3616 3703 4068 1197
Size: 2419 87 365 5746
Fragments arranged by size:
5746 2419 365 87
PfoI T'CCnGG_A
Cuts at: 1229 1238 1259 1337 3208 3647 4446 5216 1229
Size: 9 21 78 1871 439 799 770 4630
Fragments arranged by size:
4630 1871 799 770 439 78 21 9
PmeI GTTT'AAAC
Cuts at: 5614 5614
Size: 8617
PmlI CAC'GTG
Cuts at: 2628 5312 2628
Size: 2684 5933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3850 3882 4251 4283 5224 5256 7756 7788 3850
Size: 32 369 32 941 32 2500 32 4679
Fragments arranged by size:
4679 2500 941 369 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 664 1209 5121 5214 664
Size: 545 3912 93 4067
Fragments arranged by size:
4067 3912 545 93
PsiI TTA'TAA
Cuts at: 587 587
Size: 8617
PspOMI G'GGCC_C
Cuts at: 3590 4662 4691 4792 4872 5439 3590
Size: 1072 29 101 80 567 6768
Fragments arranged by size:
6768 1072 567 101 80 29
PspXI vC'TCGA_Gb
Cuts at: 2545 5035 2545
Size: 2490 6127
PstI C_TGCA'G
Cuts at: 392 924 1125 5662 6347 392
Size: 532 201 4537 685 2662
Fragments arranged by size:
4537 2662 685 532 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8617
PvuII CAG'CTG
Cuts at: 475 1126 1269 1836 5542 6040 6400 475
Size: 651 143 567 3706 498 360 2692
Fragments arranged by size:
3706 2692 651 567 498 360 143
RsrII CG'GwC_CG
Cuts at: 6810 6810
Size: 8617
SacI G_AGCT'C
Cuts at: 5330 5629 5330
Size: 299 8318
SacII CC_GC'GG
Cuts at: 3815 3815
Size: 8617
SalI G'TCGA_C
Cuts at: 673 5652 673
Size: 4979 3638
SanDI GG'GwC_CC
Cuts at: 5121 5121
Size: 8617
SapI GCTCTTCn'nnn_
Cuts at: 6638 6848 6638
Size: 210 8407
SbfI CC_TGCA'GG
Cuts at: 5662 5662
Size: 8617
ScaI AGT'ACT
Cuts at: 3439 5377 8134 3439
Size: 1938 2757 3922
Fragments arranged by size:
3922 2757 1938
SfcI C'TryA_G
Cuts at: 33 388 920 1121 2275 3143 4172 5658 6343
Size: 355 532 201 1154 868 1029 1486 685
Cuts at: 6343 7338 7529 33
Size: 995 191 1121
Fragments arranged by size:
1486 1154 1121 1029 995 868 685 532
355 201 191
SfoI GGC'GCC
Cuts at: 4940 5048 5169 5256 6047 6295 4940
Size: 108 121 87 791 248 7262
Fragments arranged by size:
7262 791 248 121 108 87
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8617
SmaI CCC'GGG
Cuts at: 274 5138 5165 5637 274
Size: 4864 27 472 3254
Fragments arranged by size:
4864 3254 472 27
SmlI C'TyrA_G
Cuts at: 1411 2127 2545 4519 5035 5770 6685 7179 7441
Size: 716 418 1974 516 735 915 494 262
Cuts at: 7441 1411
Size: 2587
Fragments arranged by size:
2587 1974 915 735 716 516 494 418
262
SnaBI TAC'GTA
Cuts at: 5607 5607
Size: 8617
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8617
SphI G_CATG'C
Cuts at: 5112 5668 6168 5112
Size: 556 500 7561
Fragments arranged by size:
7561 556 500
SspI AAT'ATT
Cuts at: 4047 4047
Size: 8617
StyI C'CwwG_G
Cuts at: 125 372 1100 1707 5552 5749 6103 125
Size: 247 728 607 3845 197 354 2639
Fragments arranged by size:
3845 2639 728 607 354 247 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 964 5464 6198 6331 6790 7001 964
Size: 4500 734 133 459 211 2580
Fragments arranged by size:
4500 2580 734 459 211 133
TatI w'GTAC_w
Cuts at: 1517 3437 5375 8132 1517
Size: 1920 1938 2757 2002
Fragments arranged by size:
2757 2002 1938 1920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 845 877 2427 2459 3962 3994 4758 4790 4828
Size: 32 1550 32 1503 32 764 32 38
Cuts at: 4828 4860 8134 8166 845
Size: 32 3274 32 1296
Fragments arranged by size:
3274 1550 1503 1296 764 38 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4857 5212 6412 4857
Size: 355 1200 7062
Fragments arranged by size:
7062 1200 355
XbaI T'CTAG_A
Cuts at: 5646 5646
Size: 8617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 144 204 1923 6110 144
Size: 60 1719 4187 2651
Fragments arranged by size:
4187 2651 1719 60
XhoI C'TCGA_G
Cuts at: 2545 5035 2545
Size: 2490 6127
XmaI C'CCGG_G
Cuts at: 272 5136 5163 5635 272
Size: 4864 27 472 3254
Fragments arranged by size:
4864 3254 472 27
XmnI GAAnn'nnTTC
Cuts at: 596 596
Size: 8617
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AscI AvrII BmtI BsiWI BsrGI BssHII
ClaI CspCI FseI FspAI NdeI NheI NotI PacI
PshAI PsrI SexAI SfiI SgrAI SrfI StuI SwaI
ZraI