Restriction map for pF1KSDA0636
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BlnI        BlpI        BmyI        Bpu1102I    BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRV       FauI        FseI        
FspI        HapII       HgaI        Hin1I       HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MluI        
MroI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        SspBI       SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI         1422  CCGC                 CCGC(-3/-1)
AfaI          299  GTAC                 GT^AC
AhdI          910  GACCCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          459  GGATC                GGATC(4/5)
Asp700I       208  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         910  GACCCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII        1177  GGACC                G^G[AT]CC
BbrPI        1207  CACGTG               CAC^GTG
BbsI         1154  GTCTTC               GAAGAC(2/6)
BclI         1289  TGATCA               T^GATCA
BglI          944  GCCCCAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         584  AGATCT               A^GATCT
BpuAI        1154  GTCTTC               GAAGAC(2/6)
BsaAI        1207  CACGTG               [CT]AC^GT[GA]
BsaI          917  GGTCTC               GGTCTC(1/5)
BseRI        1055  CTCCTC               GAGGAG(10/8)
BsmFI         987  GGGAC                GGGAC(10/14)
BsrBI        1422  CCGCTC               CCGCTC(-3/-3)
BsrDI        1115  GCAATG               GCAATG(2/0)
BstEII        907  GGTGACC              G^GT[ACGT]ACC
BstPI         907  GGTGACC              G^GT[ACGT]ACC
Cfr13I       1177  GGACC                G^G[ACGT]CC
DsaI         1388  CCATGG               C^C[GA][CT]GG
Eam1105I      910  GACCCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       907  GGTGACC              G^GT[ACGT]ACC
EcoT22I       744  ATGCAT               ATGCA^T
FbaI         1289  TGATCA               T^GATCA
HincII        116  GTCAAC               GT[CT]^[GA]AC
HindII        116  GTCAAC               GT[CT]^[GA]AC
MaeII        1208  ACGT                 A^CGT
NcoI         1388  CCATGG               C^CATGG
NlaIV         898  GGCTCC               GG[ACGT]^[ACGT]CC
NsiI          744  ATGCAT               ATGCA^T
PleI          925  GACTC                GAGTC(4/5)
PmaCI        1207  CACGTG               CAC^GTG
PmlI         1207  CACGTG               CAC^GTG
RsaI          299  GTAC                 GT^AC
Sau96I       1177  GGACC                G^G[ACGT]CC
SbfI          364  CCTGCAGG             CCTGCA^GG
SfaNI        1009  GATGC                GCATC(5/9)
Sse8387I      364  CCTGCAGG             CCTGCA^GG
SspI         1256  AATATT               AAT^ATT
StuI          574  AGGCCT               AGG^CCT
XcmI         1388  CCATGGAGTTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          208  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BalI         1211  TGGCCA               TGG^CCA
             1442  TGGCCA              
BsmAI         918  GTCTC                GTCTC(1/5)
             1418  GTCTC               
BstYI         459  GGATCT               [GA]^GATC[CT]
              584  AGATCT              
EaeI         1211  TGGCCA               [CT]^GGCC[GA]
             1442  TGGCCA              
EcoRI         490  GAATTC               G^AATTC
              786  GAATTC              
MboII         397  GAAGA                GAAGA(8/7)
             1155  TCTTC               
MflI          459  GGATCT               [GA]^GATC[CT]
              584  AGATCT              
MluNI        1211  TGGCCA               TGG^CCA
             1442  TGGCCA              
MscI         1211  TGGCCA               TGG^CCA
             1442  TGGCCA              
MslI           18  CACAAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              627  CATTAAGGTG          
MspA1I        392  CAGCTG               C[AC]G^C[GT]G
              865  CAGCTG              
PflMI        1027  CCAGCTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1536  CCAGCAGGTGG         
PstI          365  CTGCAG               CTGCA^G
             1226  CTGCAG              
PvuII         392  CAGCTG               CAG^CTG
              865  CAGCTG              
TaqI          245  TCGA                 T^CGA
              336  TCGA                
TthHB8I       245  TCGA                 T^CGA
              336  TCGA                
Van91I       1027  CCAGCTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1536  CCAGCAGGTGG         
XhoII         459  GGATCT               [GA]^GATC[CT]
              584  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BpmI        
BsaJI       BsiYI       BslI        BsmI        BspMI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
CfoI        DdeI        DpnII       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        MaeIII      
MboI        MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      Sau3AI      ScrFI       SfcI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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