Restriction map for pF1KSDA0636
 (Linear) (Six-base) MAPSORT of: KIAA0636.seq  Check: 6025  from: 1  to: 1611



KIAA0636.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1530    1611 
   Size:       1530      81
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416    1365    1611 
   Size:        416     949     246

  Fragments arranged by size: 

                 949     416     246
 
AhdI GACnn_n'nnGTC

Cuts at:      0     915    1611 
   Size:        915     696
 
AleI CACnn'nnGTG

Cuts at:      0      22    1611 
   Size:         22    1589
 
ApoI r'AATT_y

Cuts at:      0     226     490     736     786     870    1078    1611 
   Size:        226     264     246      50      84     208     533

  Fragments arranged by size: 

                 533     264     246     226     208      84      50
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1147    1611 
   Size:       1147     464
 
BclI T'GATC_A

Cuts at:      0    1289    1611 
   Size:       1289     322
 
BfrBI ATG'CAT

Cuts at:      0     746    1611 
   Size:        746     865
 
BglI GCCn_nnn'nGGC

Cuts at:      0     950    1611 
   Size:        950     661
 
BglII A'GATC_T

Cuts at:      0     584    1611 
   Size:        584    1027
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       3    1611 
   Size:          3    1608
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     702     905    1317    1611 
   Size:        702     203     412     294

  Fragments arranged by size: 

                 702     412     294     203
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     923    1611 
   Size:        923     688
 
BsaAI yAC'GTr

Cuts at:      0    1209    1611 
   Size:       1209     402
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1046    1611 
   Size:       1046     565
 
BseYI C'CCAG_C

Cuts at:      0    1535    1587    1611 
   Size:       1535      52      24

  Fragments arranged by size: 

                1535      52      24
 
BsmI GAATG_Cn'

Cuts at:      0     748     835    1494    1611 
   Size:        748      87     659     117

  Fragments arranged by size: 

                 748     659     117      87
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     372    1056    1530    1611 
   Size:        372     684     474      81

  Fragments arranged by size: 

                 684     474     372      81
 
BsrBI CCG'CTC

Cuts at:      0    1424    1611 
   Size:       1424     187
 
BsrDI GCAATG_nn'

Cuts at:      0    1122    1611 
   Size:       1122     489
 
BstEII G'GTnAC_C

Cuts at:      0     907    1611 
   Size:        907     704
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      54     447     633    1611 
   Size:         54     393     186     978

  Fragments arranged by size: 

                 978     393     186      54
 
BstYI r'GATC_y

Cuts at:      0     459     584    1611 
   Size:        459     125    1027

  Fragments arranged by size: 

                1027     459     125
 
BtgI C'CryG_G

Cuts at:      0    1388    1611 
   Size:       1388     223
 
EaeI y'GGCC_r

Cuts at:      0    1211    1442    1611 
   Size:       1211     231     169

  Fragments arranged by size: 

                1211     231     169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416     702     905    1317    1365    1611 
   Size:        416     286     203     412      48     246

  Fragments arranged by size: 

                 416     412     286     246     203      48
 
EcoRI G'AATT_C

Cuts at:      0     490     786    1611 
   Size:        490     296     825

  Fragments arranged by size: 

                 825     490     296
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     177    1522    1611 
   Size:        177    1345      89

  Fragments arranged by size: 

                1345     177      89
 
HaeII r_GCGC'y

Cuts at:      0      31    1046    1388    1611 
   Size:         31    1015     342     223

  Fragments arranged by size: 

                1015     342     223      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     901     933    1041    1073    1236    1268 
   Size:         55      32     814      32     108      32     163      32

Cuts at:   1268    1442    1474    1611 
   Size:        174      32     137

  Fragments arranged by size: 

                 814     174     163     137     108      55      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     118    1611 
   Size:        118    1493
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1341    1347    1611 
   Size:       1341       6     264

  Fragments arranged by size: 

                1341     264       6
 
MscI TGG'CCA

Cuts at:      0    1213    1444    1611 
   Size:       1213     231     167

  Fragments arranged by size: 

                1213     231     167
 
MslI CAynn'nnrTG

Cuts at:      0      22     631    1611 
   Size:         22     609     980

  Fragments arranged by size: 

                 980     609      22
 
MspA1I CmG'CkG

Cuts at:      0     394     867    1611 
   Size:        394     473     744

  Fragments arranged by size: 

                 744     473     394
 
NcoI C'CATG_G

Cuts at:      0    1388    1611 
   Size:       1388     223
 
NsiI A_TGCA'T

Cuts at:      0     748    1611 
   Size:        748     863
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1033    1542    1611 
   Size:       1033     509      69

  Fragments arranged by size: 

                1033     509      69
 
PmlI CAC'GTG

Cuts at:      0    1209    1611 
   Size:       1209     402
 
PstI C_TGCA'G

Cuts at:      0     369    1230    1611 
   Size:        369     861     381

  Fragments arranged by size: 

                 861     381     369
 
PvuII CAG'CTG

Cuts at:      0     394     867    1611 
   Size:        394     473     744

  Fragments arranged by size: 

                 744     473     394
 
SbfI CC_TGCA'GG

Cuts at:      0     369    1611 
   Size:        369    1242
 
SfcI C'TryA_G

Cuts at:      0     365    1226    1319    1611 
   Size:        365     861      93     292

  Fragments arranged by size: 

                 861     365     292      93
 
SspI AAT'ATT

Cuts at:      0    1258    1611 
   Size:       1258     353
 
StuI AGG'CCT

Cuts at:      0     576    1611 
   Size:        576    1035
 
StyI C'CwwG_G

Cuts at:      0     303     913    1125    1388    1611 
   Size:        303     610     212     263     223

  Fragments arranged by size: 

                 610     303     263     223     212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1136    1611 
   Size:       1136     475
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1395    1611 
   Size:       1395     216
 
XmnI GAAnn'nnTTC

Cuts at:      0     212    1611 
   Size:        212    1399
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AleI     ApoI     BbsI     BclI    BfrBI
     BglI    BglII     BmrI     BpmI     BsaI    BsaAI    BseRI    BseYI
     BsmI    BspMI    BsrBI    BsrDI   BstEII    BstXI    BstYI     BtgI
     EaeI  Eco57MI    EcoRI     FalI    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NcoI     NsiI    PflMI     PmlI     PstI
    PvuII     SbfI     SfcI     SspI     StuI     StyI    TaqII     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com