Restriction map for pF1KE2502
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AspI        AvrII       BbsI        BcgI        
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsiWI       BsmBI       BsmI        
BspDI       BspHI       BspLU11I    BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
CelII       ClaI        CpoI        DraI        DrdI        
DsaI        Eam1105I    Eco47III    EcoRV       EcoT22I     
FbaI        FseI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NcoI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmeI        
PshAI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SapI        SbfI        ScaI        
SfcI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        TfiI        
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccII        2013  CGCG                 CG^CG
AccIII       1994  TCCGGA               T^CCGGA
AcsI          751  GAATTC               [GA]^AATT[CT]
Alw44I       1842  GTGCAC               G^TGCAC
AlwNI        1312  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1034  GGGCCC               GGGCC^C
ApaLI        1842  GTGCAC               G^TGCAC
ApoI          751  GAATTC               [GA]^AATT[CT]
AseI         1704  ATTAAT               AT^TAAT
AsnI         1704  ATTAAT               AT^TAAT
Asp700I      1260  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         275  TGCGCA               TGC^GCA
BamHI        1991  GGATCC               G^GATCC
BbeI         1691  GGCGCC               GGCGC^C
BbrPI         897  CACGTG               CAC^GTG
BbvCI         981  CCTCAGC              CCTCAGC(-5/-2)
BciVI          22  GGATAC               GTATCC(6/5)
BglI         1556  GCCTGTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         897  CACGTG               [CT]AC^GT[GA]
BsaWI        1994  TCCGGA               [AT]^CCGG[AT]
BseAI        1994  TCCGGA               T^CCGGA
BsgI         1636  CTGCAC               GTGCAG(16/14)
BsiEI         937  CGGCCG               CG[GA][CT]^CG
BspEI        1994  TCCGGA               T^CCGGA
BsrBI        1352  GAGCGG               CCGCTC(-3/-3)
BstEII       1218  GGTGACC              G^GT[ACGT]ACC
BstPI        1218  GGTGACC              G^GT[ACGT]ACC
BstUI        2013  CGCG                 CG^CG
DraIII        472  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          937  CGGCCG               C^GGCCG
EarI         1984  GAAGAG               CTCTTC(1/4)
EclXI         937  CGGCCG               C^GGCCG
Eco52I        937  CGGCCG               C^GGCCG
EcoNI         459  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1218  GGTGACC              G^GT[ACGT]ACC
EcoRI         751  GAATTC               G^AATTC
FspI          275  TGCGCA               TGC^GCA
KasI         1691  GGCGCC               G^GCGCC
Ksp632I      1984  GAAGAG               CTCTTC(1/4)
MaeII         898  ACGT                 A^CGT
MroI         1994  TCCGGA               T^CCGGA
MseI         1705  TTAA                 T^TAA
MvnI         2013  CGCG                 CG^CG
NarI         1691  GGCGCC               GG^CGCC
NheI         1296  GCTAGC               G^CTAGC
NspI           50  GCATGC               [GA]CATG^[CT]
PmaCI         897  CACGTG               CAC^GTG
PmlI          897  CACGTG               CAC^GTG
PshBI        1704  ATTAAT               AT^TAAT
SacI          184  GAGCTC               GAGCT^C
SalI          628  GTCGAC               G^TCGAC
SexAI        1289  ACCTGGT              A^CC[AT]GGT
SfoI         1691  GGCGCC               GGC^GCC
SmaI         1127  CCCGGG               CCC^GGG
SphI           50  GCATGC               GCATG^C
StuI          581  AGGCCT               AGG^CCT
Tru9I        1705  TTAA                 T^TAA
XmaCI        1127  CCCGGG               C^CCGGG
XmaI         1127  CCCGGG               C^CCGGG
XmnI         1260  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          628  GTCGAC               GT^[AC][GT]AC
              838  GTCTAC              
AocI          778  CCTGAGG              CC^T[ACGT]AGG
             1436  CCTCAGG             
AspHI         184  GAGCTC               G[AT]GC[AT]^C
             1842  GTGCAC              
BalI          479  TGGCCA               TGG^CCA
              728  TGGCCA              
BfaI          835  CTAG                 C^TAG
             1297  CTAG                
BpmI          227  CTCCAG               CTGGAG(16/14)
               14  CTGGAG              
BsiHKAI       184  GAGCTC               G[AT]GC[AT]^C
             1842  GTGCAC              
Bsp1407I      737  TGTACA               T^GTACA
             1478  TGTACA              
BsrGI         737  TGTACA               T^GTACA
             1478  TGTACA              
BstXI          47  CCAGCATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1571  CCATATTCATGG        
Bsu36I        778  CCTGAGG              CC^T[ACGT]AGG
             1436  CCTCAGG             
Eco81I        778  CCTGAGG              CC^T[ACGT]AGG
             1436  CCTCAGG             
HincII        628  GTCGAC               GT[CT]^[GA]AC
             2002  GTCAAC              
HindII        628  GTCGAC               GT[CT]^[GA]AC
             2002  GTCAAC              
MaeI          835  CTAG                 C^TAG
             1297  CTAG                
MluNI         479  TGGCCA               TGG^CCA
              728  TGGCCA              
MscI          479  TGGCCA               TGG^CCA
              728  TGGCCA              
NaeI           83  GCCGGC               GCC^GGC
              939  GCCGGC              
NgoMIV         83  GCCGGC               G^CCGGC
              939  GCCGGC              
PaeR7I        181  CTCGAG               C^TCGAG
             2053  CTCGAG              
SmlI          181  CTCGAG               C^T[CT][GA]AG
             2053  CTCGAG              
SspBI         737  TGTACA               T^GTACA
             1478  TGTACA              
XcmI          614  CCAATGTCATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1106  CCACATCACACATGG     
XhoI          181  CTCGAG               C^TCGAG
             2053  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BglII       BmyI        BsaHI       BsaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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