Restriction map for pF1KE2502
 (Linear) (Six-base) MAPSORT of: KIEE2502.seq  Check: 2825  from: 1  to: 2172



KIEE2502.orf

 With 182 enzymes: SgfI * 

                        February 16, 2012 13:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     529    1249    2172 
   Size:        529     720     923

  Fragments arranged by size: 

                 923     720     529
 
AccI GT'mk_AC

Cuts at:      0     629     839    2172 
   Size:        629     210    1333

  Fragments arranged by size: 

                1333     629     210
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     481     679    1087    1279    2172 
   Size:        469      12     198     408     192     893

  Fragments arranged by size: 

                 893     469     408     198     192      12
 
AleI CACnn'nnGTG

Cuts at:      0    1643    1933    2172 
   Size:       1643     290     239

  Fragments arranged by size: 

                1643     290     239
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1513    1807    2172 
   Size:       1513     294     365

  Fragments arranged by size: 

                1513     365     294
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1317    2172 
   Size:       1317     855
 
ApaI G_GGCC'C

Cuts at:      0    1038    2172 
   Size:       1038    1134
 
ApaLI G'TGCA_C

Cuts at:      0    1842    2172 
   Size:       1842     330
 
ApoI r'AATT_y

Cuts at:      0     751    2172 
   Size:        751    1421
 
AseI AT'TA_AT

Cuts at:      0    1705    2172 
   Size:       1705     467
 
AvaI C'yCGr_G

Cuts at:      0     181     251    1127    2053    2172 
   Size:        181      70     876     926     119

  Fragments arranged by size: 

                 926     876     181     119      70
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2121    2154    2172 
   Size:       2121      33      18

  Fragments arranged by size: 

                2121      33      18
 
BamHI G'GATC_C

Cuts at:      0    1991    2172 
   Size:       1991     181
 
BanI G'GyrC_C

Cuts at:      0     850    1122    1497    1691    2172 
   Size:        850     272     375     194     481

  Fragments arranged by size: 

                 850     481     375     272     194
 
BanII G_rGCy'C

Cuts at:      0     188    1038    1106    2172 
   Size:        188     850      68    1066

  Fragments arranged by size: 

                1066     850     188      68
 
BbeI G_GCGC'C

Cuts at:      0    1695    2172 
   Size:       1695     477
 
BbvCI CC'TCA_GC

Cuts at:      0     982    2172 
   Size:        982    1190
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      16    2172 
   Size:         16    2156
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1562    2172 
   Size:       1562     610
 
BglII A'GATC_T

Cuts at:      0     662    1001    1550    2172 
   Size:        662     339     549     622

  Fragments arranged by size: 

                 662     622     549     339
 
Bme1580I G_kGCm'C

Cuts at:      0     529    1038    1127    1502    1736    1745    1846    2172 
   Size:        529     509      89     375     234       9     101     326

  Fragments arranged by size: 

                 529     509     375     326     234     101      89       9
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     210     866    1327    1697    2172 
   Size:        210     656     461     370     475

  Fragments arranged by size: 

                 656     475     461     370     210
 
BmtI G_CTAG'C

Cuts at:      0    1300    2172 
   Size:       1300     872
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     212    2172 
   Size:         35     177    1960

  Fragments arranged by size: 

                1960     177      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     982    1485    2172 
   Size:        982     503     687

  Fragments arranged by size: 

                 982     687     503
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      61    1399    1426    2172 
   Size:         61    1338      27     746

  Fragments arranged by size: 

                1338     746      61      27
 
BsaAI yAC'GTr

Cuts at:      0     899    2172 
   Size:        899    1273
 
BsaHI Gr'CG_yC

Cuts at:      0     794    1061    1664    1692    2172 
   Size:        794     267     603      28     480

  Fragments arranged by size: 

                 794     603     480     267      28
 
BsaWI w'CCGG_w

Cuts at:      0    1994    2172 
   Size:       1994     178
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     226     256    2172 
   Size:        226      30    1916

  Fragments arranged by size: 

                1916     226      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     247     704    1765    2172 
   Size:        247     457    1061     407

  Fragments arranged by size: 

                1061     457     407     247
 
BseYI C'CCAG_C

Cuts at:      0      46     286     762     809    2172 
   Size:         46     240     476      47    1363

  Fragments arranged by size: 

                1363     476     240      47      46
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1621    2172 
   Size:       1621     551
 
BsiEI CG_ry'CG

Cuts at:      0     940    2172 
   Size:        940    1232
 
BsiHKAI G_wGCw'C

Cuts at:      0     188    1846    2172 
   Size:        188    1658     326

  Fragments arranged by size: 

                1658     326     188
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     529    1038    1106    1127    1502    1736    1745 
   Size:        188     341     509      68      21     375     234       9

Cuts at:   1745    1846    2172 
   Size:        101     326

  Fragments arranged by size: 

                 509     375     341     326     234     188     101      68
                  21       9
 
BspEI T'CCGG_A

Cuts at:      0    1994    2172 
   Size:       1994     178
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     425     529     983    1249    2172 
   Size:        425     104     454     266     923

  Fragments arranged by size: 

                 923     454     425     266     104
 
BsrBI CCG'CTC

Cuts at:      0    1354    2172 
   Size:       1354     818
 
BsrFI r'CCGG_y

Cuts at:      0      83     925     939    1472    2172 
   Size:         83     842      14     533     700

  Fragments arranged by size: 

                 842     700     533      83      14
 
BsrGI T'GTAC_A

Cuts at:      0     737    1478    2172 
   Size:        737     741     694

  Fragments arranged by size: 

                 741     737     694
 
BstEII G'GTnAC_C

Cuts at:      0    1218    2172 
   Size:       1218     954
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      54    1578    2172 
   Size:         54    1524     594

  Fragments arranged by size: 

                1524     594      54
 
BstYI r'GATC_y

Cuts at:      0     653     662    1001    1550    1991    2172 
   Size:        653       9     339     549     441     181

  Fragments arranged by size: 

                 653     549     441     339     181       9
 
Bsu36I CC'TnA_GG

Cuts at:      0     779    1437    2172 
   Size:        779     658     735

  Fragments arranged by size: 

                 779     735     658
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     342     579    2172 
   Size:        342     237    1593

  Fragments arranged by size: 

                1593     342     237
 
BtsI GCAGTG_nn'

Cuts at:      0     527    2172 
   Size:        527    1645
 
DraIII CAC_nnn'GTG

Cuts at:      0     477    2172 
   Size:        477    1695
 
EaeI y'GGCC_r

Cuts at:      0     479     728     937    1653    2172 
   Size:        479     249     209     716     519

  Fragments arranged by size: 

                 716     519     479     249     209
 
EagI C'GGCC_G

Cuts at:      0     937    2172 
   Size:        937    1235
 
EarI CTCTTCn'nnn_

Cuts at:      0    1979    2172 
   Size:       1979     193
 
EcoICRI GAG'CTC

Cuts at:      0     186    2172 
   Size:        186    1986
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     212     469     481     679    1087    1279    2172 
   Size:         35     177     257      12     198     408     192     893

  Fragments arranged by size: 

                 893     408     257     198     192     177      35      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     463    2172 
   Size:        463    1709
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     333     351    1034    1441    2089    2172 
   Size:         42     291      18     683     407     648      83

  Fragments arranged by size: 

                 683     648     407     291      83      42      18
 
EcoRI G'AATT_C

Cuts at:      0     751    2172 
   Size:        751    1421
 
FspI TGC'GCA

Cuts at:      0     277    2172 
   Size:        277    1895
 
HaeII r_GCGC'y

Cuts at:      0     241     378    1695    2172 
   Size:        241     137    1317     477

  Fragments arranged by size: 

                1317     477     241     137
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     867     899    1093    1125    1863    1895    2172 
   Size:        867      32     194      32     738      32     277

  Fragments arranged by size: 

                 867     738     277     194      32      32      32
 
HincII GTy'rAC

Cuts at:      0     630    2004    2172 
   Size:        630    1374     168

  Fragments arranged by size: 

                1374     630     168
 
KasI G'GCGC_C

Cuts at:      0    1691    2172 
   Size:       1691     481
 
MscI TGG'CCA

Cuts at:      0     481     730    2172 
   Size:        481     249    1442

  Fragments arranged by size: 

                1442     481     249
 
MslI CAynn'nnrTG

Cuts at:      0      97    1120    1216    1576    1643    1933    2172 
   Size:         97    1023      96     360      67     290     239

  Fragments arranged by size: 

                1023     360     290     239      97      96      67
 
MspA1I CmG'CkG

Cuts at:      0     290     645    1717    2172 
   Size:        290     355    1072     455

  Fragments arranged by size: 

                1072     455     355     290
 
NaeI GCC'GGC

Cuts at:      0      85     941    2172 
   Size:         85     856    1231

  Fragments arranged by size: 

                1231     856      85
 
NarI GG'CG_CC

Cuts at:      0    1692    2172 
   Size:       1692     480
 
NgoMIV G'CCGG_C

Cuts at:      0      83     939    2172 
   Size:         83     856    1233

  Fragments arranged by size: 

                1233     856      83
 
NheI G'CTAG_C

Cuts at:      0    1296    2172 
   Size:       1296     876
 
NspI r_CATG'y

Cuts at:      0      54    2172 
   Size:         54    2118
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     477    1164    1317    1914    2172 
   Size:        477     687     153     597     258

  Fragments arranged by size: 

                 687     597     477     258     153
 
PfoI T'CCnGG_A

Cuts at:      0    1157    2172 
   Size:       1157    1015
 
PmlI CAC'GTG

Cuts at:      0     899    2172 
   Size:        899    1273
 
PpuMI rG'GwC_Cy

Cuts at:      0      42     351    2089    2172 
   Size:         42     309    1738      83

  Fragments arranged by size: 

                1738     309      83      42
 
PspOMI G'GGCC_C

Cuts at:      0    1034    2172 
   Size:       1034    1138
 
PvuII CAG'CTG

Cuts at:      0     290     645    1717    2172 
   Size:        290     355    1072     455

  Fragments arranged by size: 

                1072     455     355     290
 
SacI G_AGCT'C

Cuts at:      0     188    2172 
   Size:        188    1984
 
SalI G'TCGA_C

Cuts at:      0     628    2172 
   Size:        628    1544
 
SanDI GG'GwC_CC

Cuts at:      0      42    2089    2172 
   Size:         42    2047      83

  Fragments arranged by size: 

                2047      83      42
 
SexAI A'CCwGG_T

Cuts at:      0    1289    2172 
   Size:       1289     883
 
SfoI GGC'GCC

Cuts at:      0    1693    2172 
   Size:       1693     479
 
SmaI CCC'GGG

Cuts at:      0    1129    2172 
   Size:       1129    1043
 
SmlI C'TyrA_G

Cuts at:      0     181    2053    2172 
   Size:        181    1872     119

  Fragments arranged by size: 

                1872     181     119
 
SphI G_CATG'C

Cuts at:      0      54    2172 
   Size:         54    2118
 
StuI AGG'CCT

Cuts at:      0     583    2172 
   Size:        583    1589
 
StyI C'CwwG_G

Cuts at:      0     437     866    1223    1301    2172 
   Size:        437     429     357      78     871

  Fragments arranged by size: 

                 871     437     429     357      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     124     823    2172 
   Size:        124     699    1349

  Fragments arranged by size: 

                1349     699     124
 
TatI w'GTAC_w

Cuts at:      0     590     737    1478    2129    2172 
   Size:        590     147     741     651      43

  Fragments arranged by size: 

                 741     651     590     147      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     226     258    1438    1470    2172 
   Size:        226      32    1180      32     702

  Fragments arranged by size: 

                1180     702     226      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     621    1113    2172 
   Size:        621     492    1059

  Fragments arranged by size: 

                1059     621     492
 
XhoI C'TCGA_G

Cuts at:      0     181    2053    2172 
   Size:        181    1872     119

  Fragments arranged by size: 

                1872     181     119
 
XmaI C'CCGG_G

Cuts at:      0    1127    2172 
   Size:       1127    1045
 
XmnI GAAnn'nnTTC

Cuts at:      0    1264    2172 
   Size:       1264     908
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BglI    BglII Bme1580I     BmrI     BmtI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BstEII    BstXI    BstYI   Bsu36I    BtgZI     BtsI   DraIII
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII     KasI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NheI     NspI    PflMI     PfoI     PmlI
    PpuMI   PspOMI    PvuII     SacI     SalI    SanDI    SexAI     SfoI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI     AscI    AsiSI    AvrII     BbsI     BcgI     BclI    BfrBI
     BlpI    BmgBI     BplI    BpuEI    BsaBI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI   BssHII    BssSI   BstAPI    BstBI  BstZ17I     BtgI
     ClaI    CspCI     DraI     DrdI     EciI    EcoRV     FalI     FseI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NcoI
     NdeI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SapI     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     ZraI
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