Restriction map for pF1KSDA0608
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsgI        BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HpaI        KpnI        MamI        
MluI        NcoI        NdeI        NheI        NotI        
NsiI        NspV        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    RsrII       
SacI        SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII          9  TCCGGA               T^CCGGA
AflIII        806  ACACGT               A^C[GA][CT]GT
AlwNI         654  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AscI          718  GGCGCGCC             GG^CGCGCC
AseI         1617  ATTAAT               AT^TAAT
AsnI         1617  ATTAAT               AT^TAAT
AspHI        1001  GTGCTC               G[AT]GC[AT]^C
BalI          258  TGGCCA               TGG^CCA
BbsI         1122  GTCTTC               GAAGAC(2/6)
BbvCI        1258  GCTGAGG              CCTCAGC(-5/-2)
BpuAI        1122  GTCTTC               GAAGAC(2/6)
BsaWI           9  TCCGGA               [AT]^CCGG[AT]
BseAI           9  TCCGGA               T^CCGGA
BsiHKAI      1001  GTGCTC               G[AT]GC[AT]^C
BsmBI         169  GAGACG               CGTCTC(1/5)
BspEI           9  TCCGGA               T^CCGGA
BspMI         845  ACCTGC               ACCTGC(4/8)
BsrBI         247  CCGCTC               CCGCTC(-3/-3)
BstAPI       1428  GCAGGGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1205  CCAAATCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1982  GGATCT               [GA]^GATC[CT]
EcoRV        2167  GATATC               GAT^ATC
EcoT14I      1746  CCAAGG               C^C[AT][AT]GG
HindIII      1220  AAGCTT               A^AGCTT
MfeI         1380  CAATTG               C^AATTG
MflI         1982  GGATCT               [GA]^GATC[CT]
MluNI         258  TGGCCA               TGG^CCA
MroI            9  TCCGGA               T^CCGGA
MscI          258  TGGCCA               TGG^CCA
MunI         1380  CAATTG               C^AATTG
NruI          420  TCGCGA               TCG^CGA
NspI         1717  ACATGC               [GA]CATG^[CT]
PacI         1618  TTAATTAA             TTAAT^TAA
PflMI        2225  CCAAAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        2235  GACTCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1617  ATTAAT               AT^TAAT
PvuI          295  CGATCG               CGAT^CG
ScaI         1770  AGTACT               AGT^ACT
SmaI          911  CCCGGG               CCC^GGG
StuI         1726  AGGCCT               AGG^CCT
StyI         1746  CCAAGG               C^C[AT][AT]GG
Tsp45I       1229  GTCAC                ^GT[GC]AC
Van91I       2225  CCAAAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          996  CCATAGTGCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1982  GGATCT               [GA]^GATC[CT]
XmaCI         911  CCCGGG               C^CCGGG
XmaI          911  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Asp700I      1542  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2104  GAAGATATTC          
BbeI          268  GGCGCC               GGCGC^C
              697  GGCGCC              
BpmI          223  CTGGAG               CTGGAG(16/14)
              502  CTGGAG              
BsmAI         169  GAGAC                GTCTC(1/5)
             2276  GAGAC               
BspHI        1307  TCATGA               T^CATGA
             1683  TCATGA              
BsrDI        1761  CATTGC               GCAATG(2/0)
             1348  GCAATG              
KasI          268  GGCGCC               G^GCGCC
              697  GGCGCC              
MaeII         808  ACGT                 A^CGT
             1227  ACGT                
MaeIII        197  GTTAC                ^GT[ACGT]AC
             1229  GTCAC               
MslI         1712  CATACACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2205  CATTCAGATG          
NaeI          265  GCCGGC               GCC^GGC
              454  GCCGGC              
NarI          268  GGCGCC               GG^CGCC
              697  GGCGCC              
NgoMIV        265  GCCGGC               G^CCGGC
              454  GCCGGC              
PstI          660  CTGCAG               CTGCA^G
             2116  CTGCAG              
PvuII         220  CAGCTG               CAG^CTG
              657  CAGCTG              
RcaI         1307  TCATGA               T^CATGA
             1683  TCATGA              
SfoI          268  GGCGCC               GGC^GCC
              697  GGCGCC              
SgrAI         453  CGCCGGCG             C[GA]^CCGG[CT]G
              863  CGCCGGTG            
SspI         1336  AATATT               AAT^ATT
             1880  AATATT              
TspRI        1164  CACTG                CA[GC]TG[ACGT][ACGT]^
             1551  CAGTG               
XmnI         1542  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2104  GAAGATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApoI        AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BsaHI       BsaJI       BseRI       
BsiEI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeI        
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TthHB8I     
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