Restriction map for pF1KSDA0608
 (Linear) (Six-base) MAPSORT of: KIAA0608.seq  Check: 7096  from: 1  to: 2307



KIAA0608.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     854    2307 
   Size:        854    1453
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175    1033    2307 
   Size:        175     858    1274

  Fragments arranged by size: 

                1274     858     175
 
AflIII A'CryG_T

Cuts at:      0     806    2307 
   Size:        806    1501
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     911    1734    2307 
   Size:        911     823     573

  Fragments arranged by size: 

                 911     823     573
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2170    2202    2307 
   Size:       2170      32     105

  Fragments arranged by size: 

                2170     105      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     659    2307 
   Size:        659    1648
 
ApoI r'AATT_y

Cuts at:      0    1137    1144    2062    2307 
   Size:       1137       7     918     245

  Fragments arranged by size: 

                1137     918     245       7
 
AscI GG'CGCG_CC

Cuts at:      0     719    2307 
   Size:        719    1588
 
AseI AT'TA_AT

Cuts at:      0    1618    2307 
   Size:       1618     689
 
AvaI C'yCGr_G

Cuts at:      0      30     318     393     616     636     709     911    2307 
   Size:         30     288      75     223      20      73     202    1396

  Fragments arranged by size: 

                1396     288     223     202      75      73      30      20
            
 
BanI G'GyrC_C

Cuts at:      0      57     268     697     867    2307 
   Size:         57     211     429     170    1440

  Fragments arranged by size: 

                1440     429     211     170      57
 
BanII G_rGCy'C

Cuts at:      0     236     319     325     617     918    2307 
   Size:        236      83       6     292     301    1389

  Fragments arranged by size: 

                1389     301     292     236      83       6
 
BbeI G_GCGC'C

Cuts at:      0     272     701    2307 
   Size:        272     429    1606

  Fragments arranged by size: 

                1606     429     272
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1115    2307 
   Size:       1115    1192
 
BbvCI CC'TCA_GC

Cuts at:      0    1259    2307 
   Size:       1259    1048
 
BglI GCCn_nnn'nGGC

Cuts at:      0     180     266     314     488     736    2307 
   Size:        180      86      48     174     248    1571

  Fragments arranged by size: 

                1571     248     180     174      86      48
 
Bme1580I G_kGCm'C

Cuts at:      0     872    2307 
   Size:        872    1435
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     523    2307 
   Size:        244     279    1784

  Fragments arranged by size: 

                1784     279     244
 
Bpu10I CC'TnA_GC

Cuts at:      0    1259    2307 
   Size:       1259    1048
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586    1198    1506    2307 
   Size:        586     612     308     801

  Fragments arranged by size: 

                 801     612     586     308
 
BsaHI Gr'CG_yC

Cuts at:      0     125     172     269     302     605     698    2307 
   Size:        125      47      97      33     303      93    1609

  Fragments arranged by size: 

                1609     303     125      97      93      47      33
 
BsaWI w'CCGG_w

Cuts at:      0       9    2307 
   Size:          9    2298
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1089    1119    2307 
   Size:       1089      30    1188

  Fragments arranged by size: 

                1188    1089      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163     175     178     181     727    2307 
   Size:        163      12       3       3     546    1580

  Fragments arranged by size: 

                1580     546     163      12       3       3
 
BseYI C'CCAG_C

Cuts at:      0     312    2307 
   Size:        312    1995
 
BsiEI CG_ry'CG

Cuts at:      0     212     298     462     492     740    2307 
   Size:        212      86     164      30     248    1567

  Fragments arranged by size: 

                1567     248     212     164      86      30
 
BsiHKAI G_wGCw'C

Cuts at:      0    1005    2307 
   Size:       1005    1302
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     163    2307 
   Size:        163    2144
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     319     325     617     872     918    1005    2307 
   Size:        236      83       6     292     255      46      87    1302

  Fragments arranged by size: 

                1302     292     255     236      87      83      46       6
            
 
BspEI T'CCGG_A

Cuts at:      0       9    2307 
   Size:          9    2298
 
BspHI T'CATG_A

Cuts at:      0    1307    1683    2307 
   Size:       1307     376     624

  Fragments arranged by size: 

                1307     624     376
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     854    2307 
   Size:        854    1453
 
BsrBI CCG'CTC

Cuts at:      0     249    2307 
   Size:        249    2058
 
BsrDI GCAATG_nn'

Cuts at:      0    1355    1760    2307 
   Size:       1355     405     547

  Fragments arranged by size: 

                1355     547     405
 
BsrFI r'CCGG_y

Cuts at:      0     265     454     864    2307 
   Size:        265     189     410    1443

  Fragments arranged by size: 

                1443     410     265     189
 
BssHII G'CGCG_C

Cuts at:      0     359     448     450     719    2307 
   Size:        359      89       2     269    1588

  Fragments arranged by size: 

                1588     359     269      89       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1434    2307 
   Size:       1434     873
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1212    2307 
   Size:       1212    1095
 
BstYI r'GATC_y

Cuts at:      0    1982    2307 
   Size:       1982     325
 
BtgI C'CryG_G

Cuts at:      0      74     104     333     364     599     822     881    1449 
   Size:         74      30     229      31     235     223      59     568

Cuts at:   1449    2307 
   Size:        858

  Fragments arranged by size: 

                 858     568     235     229     223      74      59      31
                  30
 
BtsI GCAGTG_nn'

Cuts at:      0    1163    2307 
   Size:       1163    1144
 
EaeI y'GGCC_r

Cuts at:      0     209     258     354     459     489     596     737    2307 
   Size:        209      49      96     105      30     107     141    1570

  Fragments arranged by size: 

                1570     209     141     107     105      96      49      30
            
 
EagI C'GGCC_G

Cuts at:      0     209     459     489     737    2307 
   Size:        209     250      30     248    1570

  Fragments arranged by size: 

                1570     250     248     209      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     425    1401    1784    2307 
   Size:        425     976     383     523

  Fragments arranged by size: 

                 976     523     425     383
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     134    2307 
   Size:        134    2173
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     244     523    1033    2307 
   Size:        175      69     279     510    1274

  Fragments arranged by size: 

                1274     510     279     175      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0     508     570     706     892    1100    2307 
   Size:        508      62     136     186     208    1207

  Fragments arranged by size: 

                1207     508     208     186     136      62
 
EcoRV GAT'ATC

Cuts at:      0    2169    2307 
   Size:       2169     138
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1933    2307 
   Size:       1933     374
 
HaeII r_GCGC'y

Cuts at:      0     134     272     701     836    2307 
   Size:        134     138     429     135    1471

  Fragments arranged by size: 

                1471     429     138     135     134
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     162     194     541     573     810     842    1174    1206 
   Size:        162      32     347      32     237      32     332      32

Cuts at:   1206    2072    2104    2158    2190    2307 
   Size:        866      32      54      32     117

  Fragments arranged by size: 

                 866     347     332     237     162     117      54      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1220    2307 
   Size:       1220    1087
 
KasI G'GCGC_C

Cuts at:      0     268     697    2307 
   Size:        268     429    1610

  Fragments arranged by size: 

                1610     429     268
 
MfeI C'AATT_G

Cuts at:      0    1380    2307 
   Size:       1380     927
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     991    1607    2307 
   Size:        991     616     700

  Fragments arranged by size: 

                 991     700     616
 
MscI TGG'CCA

Cuts at:      0     260    2307 
   Size:        260    2047
 
MslI CAynn'nnrTG

Cuts at:      0    1716    2209    2307 
   Size:       1716     493      98

  Fragments arranged by size: 

                1716     493      98
 
MspA1I CmG'CkG

Cuts at:      0     222     256     335     366     576     601     659     687 
   Size:        222      34      79      31     210      25      58      28

Cuts at:    687     824     883    2307 
   Size:        137      59    1424

  Fragments arranged by size: 

                1424     222     210     137      79      59      58      34
                  31      28      25
 
NaeI GCC'GGC

Cuts at:      0     267     456    2307 
   Size:        267     189    1851

  Fragments arranged by size: 

                1851     267     189
 
NarI GG'CG_CC

Cuts at:      0     269     698    2307 
   Size:        269     429    1609

  Fragments arranged by size: 

                1609     429     269
 
NgoMIV G'CCGG_C

Cuts at:      0     265     454    2307 
   Size:        265     189    1853

  Fragments arranged by size: 

                1853     265     189
 
NruI TCG'CGA

Cuts at:      0     422    2307 
   Size:        422    1885
 
NspI r_CATG'y

Cuts at:      0    1721    2307 
   Size:       1721     586
 
PacI TTA_AT'TAA

Cuts at:      0    1622    2307 
   Size:       1622     685
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2231    2307 
   Size:       2231      76
 
PpuMI rG'GwC_Cy

Cuts at:      0     570     706     892    1100    2307 
   Size:        570     136     186     208    1207

  Fragments arranged by size: 

                1207     570     208     186     136
 
PshAI GACnn'nnGTC

Cuts at:      0    2239    2307 
   Size:       2239      68
 
PstI C_TGCA'G

Cuts at:      0     664    2120    2307 
   Size:        664    1456     187

  Fragments arranged by size: 

                1456     664     187
 
PvuI CG_AT'CG

Cuts at:      0     298    2307 
   Size:        298    2009
 
PvuII CAG'CTG

Cuts at:      0     222     659    2307 
   Size:        222     437    1648

  Fragments arranged by size: 

                1648     437     222
 
SacII CC_GC'GG

Cuts at:      0     336     367     602     825     884    2307 
   Size:        336      31     235     223      59    1423

  Fragments arranged by size: 

                1423     336     235     223      59      31
 
SanDI GG'GwC_CC

Cuts at:      0     706    2307 
   Size:        706    1601
 
ScaI AGT'ACT

Cuts at:      0    1772    2307 
   Size:       1772     535
 
SfcI C'TryA_G

Cuts at:      0     660    1472    1722    2116    2307 
   Size:        660     812     250     394     191

  Fragments arranged by size: 

                 812     660     394     250     191
 
SfoI GGC'GCC

Cuts at:      0     270     699    2307 
   Size:        270     429    1608

  Fragments arranged by size: 

                1608     429     270
 
SgrAI Cr'CCGG_yG

Cuts at:      0     454     864    2307 
   Size:        454     410    1443

  Fragments arranged by size: 

                1443     454     410
 
SmaI CCC'GGG

Cuts at:      0     913    2307 
   Size:        913    1394
 
SmlI C'TyrA_G

Cuts at:      0     565    1177    1521    2307 
   Size:        565     612     344     786

  Fragments arranged by size: 

                 786     612     565     344
 
SrfI GCCC'GGGC

Cuts at:      0     913    2307 
   Size:        913    1394
 
SspI AAT'ATT

Cuts at:      0    1338    1882    2307 
   Size:       1338     544     425

  Fragments arranged by size: 

                1338     544     425
 
StuI AGG'CCT

Cuts at:      0    1728    2307 
   Size:       1728     579
 
StyI C'CwwG_G

Cuts at:      0    1746    2307 
   Size:       1746     561
 
TatI w'GTAC_w

Cuts at:      0    1399    1770    2307 
   Size:       1399     371     537

  Fragments arranged by size: 

                1399     537     371
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1003    2307 
   Size:       1003    1304
 
XmaI C'CCGG_G

Cuts at:      0     911    2307 
   Size:        911    1396
 
XmnI GAAnn'nnTTC

Cuts at:      0    1546    2108    2307 
   Size:       1546     562     199

  Fragments arranged by size: 

                1546     562     199
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI     AloI    AlwNI     ApoI     AscI
     AseI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BglI
 Bme1580I     BpmI   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstXI    BstYI     BtgI
     BtsI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRV
     FalI    HaeII    Hin4I  HindIII     KasI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NruI     NspI     PacI
    PflMI    PpuMI    PshAI     PstI     PvuI    PvuII    SacII    SanDI
     ScaI     SfcI     SfoI    SgrAI     SmaI     SmlI     SrfI     SspI
     StuI     StyI     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     ApaI    ApaLI    AsiSI    AvrII     BaeI    BamHI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI     BsgI    BsiWI     BsmI    BsrGI
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI     FseI     FspI
    FspAI   HincII     HpaI     KpnI     MluI     NcoI     NdeI     NheI
     NotI     NsiI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI    RsrII     SacI     SalI     SapI
     SbfI    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     ZraI
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