Restriction map for pF1KSDA0592
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AviII       AvrII       BamHI       BbrPI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaI        BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        Eco47III    EcoT22I     FbaI        
FseI        FspI        HindIII     KpnI        KspI        
MfeI        MluI        MroI        MunI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         3050  GTAGAC               GT^[AC][GT]AC
AccII         120  CGCG                 CG^CG
AhdI         1912  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1912  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1773  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2953  CCCGGG               C^[CT]CG[GA]G
BalI         3168  TGGCCA               TGG^CCA
BbeI         3151  GGCGCC               GGCGC^C
BcgI         2484  CGATCCTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3842  GTATCC               GTATCC(6/5)
BsaWI           8  ACCGGA               [AT]^CCGG[AT]
BsoBI        2953  CCCGGG               C^[CT]CG[GA]G
BspMI        1916  GCAGGT               ACCTGC(4/8)
BsrBI          58  GAGCGG               CCGCTC(-3/-3)
BssSI         362  CACGAG               CACGAG(-5/-1)
BstEII       1548  GGTTACC              G^GT[ACGT]ACC
BstPI        1548  GGTTACC              G^GT[ACGT]ACC
BstUI         120  CGCG                 CG^CG
CfoI         3152  GCGC                 GCG^C
DraIII       3910  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1912  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1548  GGTTACC              G^GT[ACGT]ACC
EcoRI         145  GAATTC               G^AATTC
HaeII        3151  GGCGCC               [GA]GCGC^[CT]
HhaI         3152  GCGC                 GCG^C
HinP1I       3152  GCGC                 G^CGC
HpaI         2728  GTTAAC               GTT^AAC
KasI         3151  GGCGCC               G^GCGCC
MaeII        1702  ACGT                 A^CGT
MluNI        3168  TGGCCA               TGG^CCA
MscI         3168  TGGCCA               TGG^CCA
MvnI          120  CGCG                 CG^CG
NaeI           36  GCCGGC               GCC^GGC
NarI         3151  GGCGCC               GG^CGCC
NdeI         2787  CATATG               CA^TATG
NgoMIV         36  GCCGGC               G^CCGGC
PflFI        1773  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI         1819  GAGCTC               GAGCT^C
SapI         2527  GAAGAGC              GCTCTTC(1/4)
ScaI          321  AGTACT               AGT^ACT
SfoI         3151  GGCGCC               GGC^GCC
SmaI         2953  CCCGGG               CCC^GGG
SspI         1879  AATATT               AAT^ATT
TaqI          909  TCGA                 T^CGA
Tth111I      1773  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       909  TCGA                 T^CGA
XmaCI        2953  CCCGGG               C^CCGGG
XmaI         2953  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI           39  GGCGTC               G[GA]^CG[CT]C
             3151  GGCGCC              
AlwNI          25  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1217  CAGGAGCTG           
AocI          938  CCTCAGG              CC^T[ACGT]AGG
             2806  CCTCAGG             
Asp700I       989  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2758  GAATCCATTC          
AspHI        1819  GAGCTC               G[AT]GC[AT]^C
             2505  GAGCAC              
BbvCI         331  GCTGAGG              CCTCAGC(-5/-2)
             3103  GCTGAGG             
BsaBI        2650  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3478  GATGATGATC          
BsaHI          39  GGCGTC               G[GA]^CG[CT]C
             3151  GGCGCC              
BsiEI          61  CGGCCG               CG[GA][CT]^CG
              113  CGGCCG              
BsiHKAI      1819  GAGCTC               G[AT]GC[AT]^C
             2505  GAGCAC              
BsmI         3942  GAATGC               GAATGC(1/-1)
             2334  GCATTC              
BsrDI        2025  CATTGC               GCAATG(2/0)
             3132  CATTGC              
BsrFI          36  GCCGGC               [GA]^CCGG[CT]
             2968  GCCGGT              
Bsu36I        938  CCTCAGG              CC^T[ACGT]AGG
             2806  CCTCAGG             
Cfr10I         36  GCCGGC               [GA]^CCGG[CT]
             2968  GCCGGT              
EagI           61  CGGCCG               C^GGCCG
              113  CGGCCG              
EclXI          61  CGGCCG               C^GGCCG
              113  CGGCCG              
Eco52I         61  CGGCCG               C^GGCCG
              113  CGGCCG              
Eco81I        938  CCTCAGG              CC^T[ACGT]AGG
             2806  CCTCAGG             
EcoNI        3241  CCTTGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3454  CCTGCTCTAGG         
HgaI          118  GACGC                GACGC(5/10)
               40  GCGTC               
Hin1I          39  GGCGTC               G[GA]^CG[CT]C
             3151  GGCGCC              
HincII       2575  GTTGAC               GT[CT]^[GA]AC
             2728  GTTAAC              
HindII       2575  GTTGAC               GT[CT]^[GA]AC
             2728  GTTAAC              
HphI         1366  GGTGA                GGTGA(8/7)
             1450  GGTGA               
MamI         2650  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3478  GATGATGATC          
NcoI          902  CCATGG               C^CATGG
             2362  CCATGG              
PvuII        1757  CAGCTG               CAG^CTG
             3227  CAGCTG              
SmlI          325  CTCAAG               C^T[CT][GA]AG
             1573  CTCAAG              
XmnI          989  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2758  GAATCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoRV       EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XcmI        
XhoII       
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