Restriction map for pF1KSDA0582
 (Linear) (Six-base) MAPSORT of: KIAA0582.seq  Check: 1303  from: 1  to: 2271



KIAA0582.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AccI GT'mk_AC

Cuts at:      0    1001    2271 
   Size:       1001    1270
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59    2062    2271 
   Size:         59    2003     209

  Fragments arranged by size: 

                2003     209      59
 
AflII C'TTAA_G

Cuts at:      0    1123    2271 
   Size:       1123    1148
 
AgeI A'CCGG_T

Cuts at:      0    1793    2271 
   Size:       1793     478
 
AleI CACnn'nnGTG

Cuts at:      0    2258    2271 
   Size:       2258      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     402     489    2040    2085    2271 
   Size:        402      87    1551      45     186

  Fragments arranged by size: 

                1551     402     186      87      45
 
ApaI G_GGCC'C

Cuts at:      0     107    1109    2271 
   Size:        107    1002    1162

  Fragments arranged by size: 

                1162    1002     107
 
AvaI C'yCGr_G

Cuts at:      0     931    1738    2271 
   Size:        931     807     533

  Fragments arranged by size: 

                 931     807     533
 
BamHI G'GATC_C

Cuts at:      0    1498    1647    2271 
   Size:       1498     149     624

  Fragments arranged by size: 

                1498     624     149
 
BanI G'GyrC_C

Cuts at:      0    1382    2271 
   Size:       1382     889
 
BanII G_rGCy'C

Cuts at:      0     107     152     234     269     559    1109    1209    1303 
   Size:        107      45      82      35     290     550     100      94

Cuts at:   1303    1552    2271 
   Size:        249     719

  Fragments arranged by size: 

                 719     550     290     249     107     100      94      82
                  45      35
 
BbeI G_GCGC'C

Cuts at:      0    1386    2271 
   Size:       1386     885
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      47     370    1888    1979    2271 
   Size:         47     323    1518      91     292

  Fragments arranged by size: 

                1518     323     292      91      47
 
BbvCI CC'TCA_GC

Cuts at:      0     140     496     938    2271 
   Size:        140     356     442    1333

  Fragments arranged by size: 

                1333     442     356     140
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1429    1463    2271 
   Size:       1429      34     808

  Fragments arranged by size: 

                1429     808      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     173    2271 
   Size:        173    2098
 
BglII A'GATC_T

Cuts at:      0     900    2271 
   Size:        900    1371
 
BlpI GC'TnA_GC

Cuts at:      0     553     572    2271 
   Size:        553      19    1699

  Fragments arranged by size: 

                1699     553      19
 
Bme1580I G_kGCm'C

Cuts at:      0     107    1109    1380    1695    2271 
   Size:        107    1002     271     315     576

  Fragments arranged by size: 

                1002     576     315     271     107
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      48     732     828    1538    2095    2271 
   Size:         48     684      96     710     557     176

  Fragments arranged by size: 

                 710     684     557     176      96      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1648    2271 
   Size:       1648     623
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     604     703    1010    1122    1584    1643    2170 
   Size:        347     257      99     307     112     462      59     527

Cuts at:   2170    2271 
   Size:        101

  Fragments arranged by size: 

                 527     462     347     307     257     112     101      99
                  59
 
Bpu10I CC'TnA_GC

Cuts at:      0     140     432     496     516     938    1312    2271 
   Size:        140     292      64      20     422     374     959

  Fragments arranged by size: 

                 959     422     374     292     140      64      20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1966    2271 
   Size:       1966     305
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     323     526     675    1749    2271 
   Size:        323     203     149    1074     522

  Fragments arranged by size: 

                1074     522     323     203     149
 
BsaBI GATnn'nnATC

Cuts at:      0    2030    2271 
   Size:       2030     241
 
BsaHI Gr'CG_yC

Cuts at:      0     795    1383    2271 
   Size:        795     588     888

  Fragments arranged by size: 

                 888     795     588
 
BsaWI w'CCGG_w

Cuts at:      0    1793    2271 
   Size:       1793     478
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     850     880    2271 
   Size:        850      30    1391

  Fragments arranged by size: 

                1391     850      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     400     663     666    1737    1859    2271 
   Size:        400     263       3    1071     122     412

  Fragments arranged by size: 

                1071     412     400     263     122       3
 
BseYI C'CCAG_C

Cuts at:      0    1307    2271 
   Size:       1307     964
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    1377    2196    2271 
   Size:        275    1102     819      75

  Fragments arranged by size: 

                1102     819     275      75
 
BsiEI CG_ry'CG

Cuts at:      0     692     861    2271 
   Size:        692     169    1410

  Fragments arranged by size: 

                1410     692     169
 
BsiHKAI G_wGCw'C

Cuts at:      0    2255    2271 
   Size:       2255      16
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     786    2271 
   Size:        786    1485
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     152     234     269     559    1109    1209    1303 
   Size:        107      45      82      35     290     550     100      94

Cuts at:   1303    1380    1552    1695    2255    2271 
   Size:         77     172     143     560      16

  Fragments arranged by size: 

                 560     550     290     172     143     107     100      94
                  82      77      45      35      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     192    1399    2271 
   Size:        192    1207     872

  Fragments arranged by size: 

                1207     872     192
 
BsrBI CCG'CTC

Cuts at:      0      84    1459    2271 
   Size:         84    1375     812

  Fragments arranged by size: 

                1375     812      84
 
BsrDI GCAATG_nn'

Cuts at:      0    1879    2252    2271 
   Size:       1879     373      19

  Fragments arranged by size: 

                1879     373      19
 
BsrFI r'CCGG_y

Cuts at:      0     687    1268    1793    2003    2271 
   Size:        687     581     525     210     268

  Fragments arranged by size: 

                 687     581     525     268     210
 
BssSI C'ACGA_G

Cuts at:      0     647    2271 
   Size:        647    1624
 
BstEII G'GTnAC_C

Cuts at:      0     625     743    1515    2271 
   Size:        625     118     772     756

  Fragments arranged by size: 

                 772     756     625     118
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      61    1642    2271 
   Size:         61    1581     629

  Fragments arranged by size: 

                1581     629      61
 
BstYI r'GATC_y

Cuts at:      0     900    1498    1639    1647    2271 
   Size:        900     598     141       8     624

  Fragments arranged by size: 

                 900     624     598     141       8
 
Bsu36I CC'TnA_GG

Cuts at:      0    1252    1652    2271 
   Size:       1252     400     619

  Fragments arranged by size: 

                1252     619     400
 
BtgI C'CryG_G

Cuts at:      0     317    1947    2237    2271 
   Size:        317    1630     290      34

  Fragments arranged by size: 

                1630     317     290      34
 
BtsI GCAGTG_nn'

Cuts at:      0    1085    1135    2271 
   Size:       1085      50    1136

  Fragments arranged by size: 

                1136    1085      50
 
DraIII CAC_nnn'GTG

Cuts at:      0     298    2271 
   Size:        298    1973
 
EaeI y'GGCC_r

Cuts at:      0     662    1798    2271 
   Size:        662    1136     473

  Fragments arranged by size: 

                1136     662     473
 
EarI CTCTTCn'nnn_

Cuts at:      0    1354    1412    1898    2271 
   Size:       1354      58     486     373

  Fragments arranged by size: 

                1354     486     373      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     655    2271 
   Size:        655    1616
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     347     604     703    1010    1122    1584    1643 
   Size:         59     288     257      99     307     112     462      59

Cuts at:   1643    2062    2170    2271 
   Size:        419     108     101

  Fragments arranged by size: 

                 462     419     307     288     257     112     108     101
                  99      59      59
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1341    2271 
   Size:       1341     930
 
EcoO109I rG'GnC_Cy

Cuts at:      0     103     727    1734    2271 
   Size:        103     624    1007     537

  Fragments arranged by size: 

                1007     624     537     103
 
EcoRV GAT'ATC

Cuts at:      0    1536    2271 
   Size:       1536     735
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     922    1846    2271 
   Size:        922     924     425

  Fragments arranged by size: 

                 924     922     425
 
HaeII r_GCGC'y

Cuts at:      0    1386    2271 
   Size:       1386     885
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     137     169     448     480     850     882    1000    1032 
   Size:        137      32     279      32     370      32     118      32

Cuts at:   1032    1285    1317    2271 
   Size:        253      32     954

  Fragments arranged by size: 

                 954     370     279     253     137     118      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     370    2271 
   Size:        370    1901
 
KasI G'GCGC_C

Cuts at:      0    1382    2271 
   Size:       1382     889
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1769    2069    2271 
   Size:       1769     300     202

  Fragments arranged by size: 

                1769     300     202
 
MscI TGG'CCA

Cuts at:      0     664    1800    2271 
   Size:        664    1136     471

  Fragments arranged by size: 

                1136     664     471
 
MslI CAynn'nnrTG

Cuts at:      0    2258    2271 
   Size:       2258      13
 
MspA1I CmG'CkG

Cuts at:      0     584     973    1899    2271 
   Size:        584     389     926     372

  Fragments arranged by size: 

                 926     584     389     372
 
NarI GG'CG_CC

Cuts at:      0    1383    2271 
   Size:       1383     888
 
NcoI C'CATG_G

Cuts at:      0     317    2237    2271 
   Size:        317    1920      34

  Fragments arranged by size: 

                1920     317      34
 
NspI r_CATG'y

Cuts at:      0    2202    2271 
   Size:       2202      69
 
PasI CC'CwG_GG

Cuts at:      0       6     100    2271 
   Size:          6      94    2171

  Fragments arranged by size: 

                2171      94       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     489    1102    2271 
   Size:        489     613    1169

  Fragments arranged by size: 

                1169     613     489
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     546     578    2271 
   Size:        546      32    1693

  Fragments arranged by size: 

                1693     546      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     727    2271 
   Size:        727    1544
 
PspOMI G'GGCC_C

Cuts at:      0     103    1105    2271 
   Size:        103    1002    1166

  Fragments arranged by size: 

                1166    1002     103
 
PstI C_TGCA'G

Cuts at:      0      79     589     989    1959    1997    2271 
   Size:         79     510     400     970      38     274

  Fragments arranged by size: 

                 970     510     400     274      79      38
 
PvuII CAG'CTG

Cuts at:      0     584     973    1899    2271 
   Size:        584     389     926     372

  Fragments arranged by size: 

                 926     584     389     372
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1898    2271 
   Size:       1898     373
 
SbfI CC_TGCA'GG

Cuts at:      0     989    2271 
   Size:        989    1282
 
ScaI AGT'ACT

Cuts at:      0     817    2271 
   Size:        817    1454
 
SfcI C'TryA_G

Cuts at:      0      75     585     985    1277    1955    1993    2271 
   Size:         75     510     400     292     678      38     278

  Fragments arranged by size: 

                 678     510     400     292     278      75      38
 
SfoI GGC'GCC

Cuts at:      0    1384    2271 
   Size:       1384     887
 
SmaI CCC'GGG

Cuts at:      0     933    2271 
   Size:        933    1338
 
SmlI C'TyrA_G

Cuts at:      0    1123    1981    2271 
   Size:       1123     858     290

  Fragments arranged by size: 

                1123     858     290
 
SrfI GCCC'GGGC

Cuts at:      0     933    2271 
   Size:        933    1338
 
StyI C'CwwG_G

Cuts at:      0     317     665    1511    1553    1635    2118    2237    2271 
   Size:        317     348     846      42      82     483     119      34

  Fragments arranged by size: 

                 846     483     348     317     119      82      42      34
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2270    2271 
   Size:       2270       1
 
TatI w'GTAC_w

Cuts at:      0     815    2271 
   Size:        815    1456
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1315    1347    2271 
   Size:       1315      32     924

  Fragments arranged by size: 

                1315     924      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     979    2271 
   Size:        979    1292
 
XbaI T'CTAG_A

Cuts at:      0     226    2271 
   Size:        226    2045
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1156    1509    1618    2244    2271 
   Size:       1156     353     109     626      27

  Fragments arranged by size: 

                1156     626     353     109      27
 
XmaI C'CCGG_G

Cuts at:      0     931    2271 
   Size:        931    1340
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AgeI     AleI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI
    HaeII    Hin4I  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NspI     PasI    PflMI     PpiI    PpuMI   PspOMI
     PstI    PvuII     SapI     SbfI     ScaI     SfcI     SfoI     SmaI
     SmlI     SrfI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AhdI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BclI    BfrBI     BglI    BmgBI     BmtI    BsaAI    BsiWI     BsmI
    BspEI    BspHI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRI     FseI
     FspI    FspAI   HincII     HpaI     KpnI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     StuI     SwaI     XhoI
     XmnI     ZraI
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